Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,836,483 |
T→C |
66.2% |
V279V (GTA→GTG) |
yjeK ← |
predicted lysine aminomutase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,836,483 | 0 | T | C | 66.2%
| 103.8
/ 72.9
| 130 | V279V (GTA→GTG) | yjeK | predicted lysine aminomutase |
| Reads supporting (aligned to +/- strand): ref base T (31/13); new base C (33/53); total (64/66) |
| Fisher's exact test for biased strand distribution p-value = 7.70e-04 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
Only 100 of 130 total aligned reads displayed.
CACTCACCATAAAATGCGCCGCGCCCTGTACTTTATCGAGCACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCACATC > minE/2836422‑2836545
|
cactcacCATAAAATGCGCCGCGCCCTGTACTTTATCGAGCACATGCAGGTAATACGGCATTACGCCgg < 1:2430639/69‑1 (MQ=255)
cactcacCATAAAATGCGCCGCGCCCTGTACTTTATCGAGCACATGCAGGTAATACGGCATTACGCCgg < 1:31688/69‑1 (MQ=255)
cactcacCATAAAATGCGCCGCGCCCTGTACTTTATCGAGCACATGCAGGTAATACGGCATTACGCCgg < 1:1226007/69‑1 (MQ=255)
cactcacCATAAAATGCGCCGCGCCCTGTACTTTATCGAGCACATGCAGGTAATACGGCATTACGCCgg < 1:1671870/69‑1 (MQ=255)
cactcacCATAAAATGCGCCGCGCCCTGTACTTTATCGAGCACATGCAGGTAATACGGCATTACGCCgg < 1:287414/69‑1 (MQ=255)
cactcacCATAAAATGCGCCGCGCCCTGTACTTTATCGAGCACATGCAGGTAATACGGCATTACGCCgg < 1:1570523/69‑1 (MQ=255)
cactcacCATAAAATGCGCCGCGCCCTGTACTTTATCGAGCACATGCAGGTAATACGGCATTACGCCgg < 1:2958768/69‑1 (MQ=255)
aTAAAATGCGCCGCGCCCTGTACTTTATCGAGCACATGCAGGTAATACGGCATTACGCCGGCATCGaac < 1:3265966/69‑1 (MQ=255)
aaaTGCGCCGCGCCCTGTACTTTATCGAGCACATGCAGGTAATACGGCATTACGCCGGCATCGaacaa > 1:599726/1‑68 (MQ=255)
aaaTGCGCCGCGCCCTGTACTTTATCGAGCACATGCAGGTAATACGGCATTACGCCGGCATCGaacaa > 1:2038114/1‑68 (MQ=255)
aaaTGCGCCGCGCCCTGTACTTTATCGAGCACATGCAGGTAATACGGCATTACGCCGGCATCGaacaa > 1:749700/1‑68 (MQ=255)
aaaTGCGCCGCGCCCTGTACTTTATCGAGCACATGCAGGTAATACGGCATTACGCCGGCATCGaacaa > 1:1593874/1‑68 (MQ=255)
aaaTGCGCCGCGCCCTGTACTTTATCGAGAACATGCAGGTAATACGGCATTACGCCGGCATCGaac > 1:1019044/1‑66 (MQ=255)
tACTTTATCGAGCACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAgg > 1:620/1‑64 (MQ=255)
tACTTTATCGAGCACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGttt > 1:154598/1‑67 (MQ=255)
tACTTTATCGAGCACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGttt > 1:297921/1‑67 (MQ=255)
cTTTATCGAGCACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCa > 1:1674842/1‑69 (MQ=255)
cTTTATCGAGCACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCa > 1:798947/1‑69 (MQ=255)
cTTTATCGAGCACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCa > 1:1819382/1‑69 (MQ=255)
cTTTATCGAGCACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCa > 1:602290/1‑69 (MQ=255)
cGAGCACATGCAGGTAATACGGCATCACGCCCGCATCGAACAACGCATTACTCAGGTTTGCCAGCGttt > 1:434348/1‑69 (MQ=255)
cGAGCACATGCAGGTAATACGGCATCACGCCCGCATCGAACAACGCATTACTCAGGTTTGCCAGCGttt > 1:2264688/1‑69 (MQ=255)
cGAGCACATGCAGGTAATACGGCATCACGCCCGCATCGAACAACGCATTACTCAGGTTTGCCAGCGttt > 1:942999/1‑69 (MQ=255)
cGAGCACATGCAGGTAATACGGCATCACGCCCGCATCGAACAACGCATTACTCAGGTTTGCCAGCGttt > 1:3087186/1‑69 (MQ=255)
cGAGCACATGCAGGTAATACGGCATCACGCCCGCATCGAACAACGCATTACTCAGGTTTGCCAGCGttt > 1:205650/1‑69 (MQ=255)
cGAGCACATGCAGGTAATACGGCATCACGCCCGCATCGAACAACGCATTACTCAGGTTTGCCAGCGttt > 1:3330262/1‑69 (MQ=255)
cGAGCACATGCAGGTAATACGGCATCACGCCCGCATCGAACAACGCATTACTCAGGTTTGCCAGCGttt > 1:3365470/1‑69 (MQ=255)
cGAGCACATGCAGGTAATACGGCATCACGCCCGCATCGAACAACGCATTACTCAGGTTTGCCAGCGtt > 1:1241890/1‑68 (MQ=255)
aTGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGtt > 1:2439921/1‑69 (MQ=255)
aTGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGtt > 1:2969048/1‑69 (MQ=255)
aTGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGtt > 1:8713/1‑69 (MQ=255)
aTGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGtt > 1:187123/1‑69 (MQ=255)
aTGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGtt > 1:1690016/1‑69 (MQ=255)
aTGCAGGTAATACGGCATTACGCCGGCATCGAACAAAGCATTACTCAGGTTTGCCAGCGTTTGTGCGtt > 1:1223375/1‑69 (MQ=255)
ggTAATACGGCATCACGCCCGCATCGAACAACGCATTACTCAGTTTTGCCAGCGTTTGCGCGTTATCg < 1:1664241/68‑1 (MQ=255)
ggTAATACGGCATCACGCCCGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGCGCGTTATCg < 1:3189984/68‑1 (MQ=255)
ggTAATACGGCATCACGCCCGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGCGCGTTATCg < 1:3306582/68‑1 (MQ=255)
ggTAATACGGCATCACGCCCGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGCGCGTTATCg < 1:2525703/68‑1 (MQ=255)
ggTAATACGGCATCACGCCCGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGCGCGTTATCg < 1:2665164/68‑1 (MQ=255)
ggTAATACGGCATCACGCCCGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGCGCGTTATCg < 1:496475/68‑1 (MQ=255)
ggTAATACGGCATCACGCCCGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGCGCGTTATCg < 1:487827/68‑1 (MQ=255)
ggTAATACGGCATCACGCCCGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGCGCGTTATCg < 1:3040287/68‑1 (MQ=255)
ggTAATACGGCATCACGCCCGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGCGCGTTATCg < 1:1054738/68‑1 (MQ=255)
ggTAATACGGCATCACGCCCGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGCGCGTTATCg < 1:1610757/68‑1 (MQ=255)
ggTAATACGGCATCACGCCCGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGCGCGTTATCg < 1:1408956/68‑1 (MQ=255)
ggTAATACGGCATCACGCCCGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGCGCGTTATCg < 1:1405045/68‑1 (MQ=255)
ggTAATACGGCATCACGCCCGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGCGCGTTATCg < 1:1220154/68‑1 (MQ=255)
ggTAATACGGCATCACGCCCGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGCGCGTTATCg < 1:1181463/68‑1 (MQ=255)
ggTAATACGGCATCACGCCCGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGCGCGTTATCg < 1:2077750/68‑1 (MQ=255)
ggTAATACGGCATCACGCCCGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGCGCGTTATCg < 1:2083445/68‑1 (MQ=255)
ggTAATACGGCATCACGCCCGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGCCCGTTATCg < 1:3255068/68‑1 (MQ=255)
ggTAATACGGCATCACGCCCGAATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGCGCGTTATCg < 1:1650471/68‑1 (MQ=255)
gTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGtt < 1:2112080/69‑1 (MQ=255)
gTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGtt < 1:1620800/69‑1 (MQ=255)
gTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGtt < 1:3363283/69‑1 (MQ=255)
aaTACGGCATCACGCCCGCATCGAACAACGCATTACt > 1:3310230/1‑37 (MQ=38)
aaTACGGCATCACGCCCGCATCGAACAACGCATTACt > 1:1097240/1‑37 (MQ=38)
aaTACGGCATCACGCCCGCATCGAACAACGCATTACt > 1:922207/1‑37 (MQ=38)
aaTACGGCATCACGCCCGCATCGAACAACGCATTACt > 1:3252704/1‑37 (MQ=38)
aaTACGGCATCACGCCCGCATCGAACAACGCATTACt > 1:2357477/1‑37 (MQ=38)
aaTACGGCATCACGCCCGCATCGAACAACGCATTACt > 1:899429/1‑37 (MQ=38)
aaTACGGCATCACGCCCGCATCGAACAACGCATTACt > 1:1538619/1‑37 (MQ=38)
aaTACGGCATCACGCCCGCATCGAACAACGCATTACt > 1:1576160/1‑37 (MQ=38)
aaTACGGCATCACGCCCGCATCGAACAACGCATTACt > 1:2861450/1‑37 (MQ=38)
aaTACGGCATCACGCCCGCATCGAACAACGCATTACt > 1:2798753/1‑37 (MQ=38)
aaTACGGCATCACGCCCGCATCGAACAACGCATTACt > 1:48579/1‑37 (MQ=38)
aaTACGGCATCACGCCCGCATCGAACAACGCATTACt > 1:865259/1‑37 (MQ=38)
aaTACGGCATCACGCCCGCATCGAACAACGCATTACt > 1:2667918/1‑37 (MQ=38)
aaTACGGCATCACGCCCGCATCGAACAACGCATTACt > 1:520911/1‑37 (MQ=38)
aaTACGGCATCACGCCCGCATCGAACAACGCATTACt > 1:2204730/1‑37 (MQ=38)
aaTACGGCATCACGCCCGCATCGAACAACGCATTACt > 1:2282212/1‑37 (MQ=38)
aaTACGGCATCACGCCCGCATCGAACAACGCATTACTCAGGTTTGCCAGCGt < 1:862962/52‑1 (MQ=255)
aaTACGGCATCACGCCCGCATCGAACAACGCATTACTCAGGTTTGCCAGCGt < 1:630055/52‑1 (MQ=255)
aaTACGGCATCACGCCCGCATCGAACAACGCATTACTCAGGTTTGCCAGCGt < 1:968321/52‑1 (MQ=255)
aaTACGGCATCACGCCCGCATCGAACAACGCATTACTCAGGTTTGCCAGCGt < 1:3192648/52‑1 (MQ=255)
aaTACGGCATCACGCCCGCATCGAACAACGCATTACTCAGGTTTGCCAGCGt < 1:1035051/52‑1 (MQ=255)
aaTACGGCATCACGCCCGCATCGAACAACGCATTACTCAGGTTTGCCAGCGt < 1:1286156/52‑1 (MQ=255)
aaTACGGCATCACGCCCGCATCGAACAACGCATTACTCAGGTTTGCCAGCGt < 1:1357578/52‑1 (MQ=255)
aaTACGGCATCACGCCCGCATCGAACAACGCATTACTCAGGTTTGCCAGCGt < 1:1439721/52‑1 (MQ=255)
aaTACGGCATCACGCCCGCATCGAACAACGCATTACTCAGGTTTGCCAGCGt < 1:1716713/52‑1 (MQ=255)
aaTACGGCATCACGCCCGCATCGAACAACGCATTACTCAGGTTTGCCAGCGt < 1:2169912/52‑1 (MQ=255)
aaTACGGCATCACGCCCGCATCGAACAACGCATTACTCAGGTTTGCCAGCGt < 1:253241/52‑1 (MQ=255)
aaTACGGCATCACGCCCGCATCGAACAACGCATTACTCAGGTTTGCCAGCGt < 1:2569465/52‑1 (MQ=255)
aaTACGGCATCACGCCCGCATCGAACAACGCATTACTCAGGTTTGCCAGCGt < 1:3285503/52‑1 (MQ=255)
aaTACGGCATCACGCCCGCATCGAACAACGCATTACTCAGGTTTGCCAGCGt < 1:2968190/52‑1 (MQ=255)
aaTACGGCATCACGCCCGCATCGAACAACGCATTACTCAGGTTTGCCAGCGt < 1:2886750/52‑1 (MQ=255)
aaTACGGCATCACGCCCGCATCGAACAACGCATTACTCAGGTTTGCCAGCGt < 1:2774197/52‑1 (MQ=255)
aaTACGGCATCACGCCCGCATCGAACAACGCATTACTCAGGTTTGCCAGCGt < 1:281635/52‑1 (MQ=255)
aaTACGGCATCACGCCCGCATCGAACAACGCATTACTCAGGTTAGCCAGCGt < 1:1552424/52‑1 (MQ=255)
aaTACGGCATCACGCCCGCATCGAACAACGAATTACt > 1:1939076/1‑37 (MQ=25)
aTACGGCATCACGCCCGCATCGAACAACGCATTACTCAGGTTTGCCAGCGt < 1:579266/51‑1 (MQ=255)
aTACGGCATCACGCCCGCATCGAACAACGCATTACTCAGGTTTGCCAGCGt < 1:230221/51‑1 (MQ=255)
aCGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCACAt > 1:241497/1‑69 (MQ=255)
aCGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCACAt > 1:3026657/1‑69 (MQ=255)
aCGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCACAt > 1:963285/1‑69 (MQ=255)
cGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCACATc > 1:1741336/1‑69 (MQ=255)
cGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCACATc > 1:2011956/1‑69 (MQ=255)
cGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCACATc > 1:2729465/1‑69 (MQ=255)
cGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCACATc > 1:271876/1‑69 (MQ=255)
ggCATCACGCCCGCATCGAACAACGCATTACTCAGGTTTGCCAGCGt < 1:918620/47‑1 (MQ=39)
|
CACTCACCATAAAATGCGCCGCGCCCTGTACTTTATCGAGCACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCACATC > minE/2836422‑2836545
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
