Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,848,531 |
C→T |
45.3% |
V834V (GTG→GTA) |
yjeP ← |
predicted mechanosensitive channel |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,848,531 | 0 | C | T | 45.3%
| 16.5
/ 69.3
| 86 | V834V (GTG→GTA) | yjeP | predicted mechanosensitive channel |
| Reads supporting (aligned to +/- strand): ref base C (11/36); new base T (27/12); total (38/48) |
| Fisher's exact test for biased strand distribution p-value = 2.77e-05 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
TCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACA > minE/2848466‑2848595
|
tCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAgcac > 1:3394587/1‑68 (MQ=255)
tCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAgcac > 1:3093044/1‑68 (MQ=255)
gcgggcggGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGTACCGCACCGAg > 1:2540/1‑68 (MQ=255)
gcgGGCAGGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGTACCGCACCGa > 1:2521609/1‑67 (MQ=255)
gcgGGCAGGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGTACCGCACCGAg > 1:2605357/1‑68 (MQ=255)
gcgGGCAGGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGTACCGCACCGAg > 1:147849/1‑68 (MQ=255)
gcgGGCAGGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGTACCGCACCGAg > 1:1563571/1‑68 (MQ=255)
gcgGGCAGGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGTACCGCACCGAg > 1:1580682/1‑68 (MQ=255)
gcgGGCAGGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGTACCGCACCGAg > 1:160038/1‑68 (MQ=255)
gcgGGCAGGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGTACCGCACCGAg > 1:164836/1‑68 (MQ=255)
gcgGGCAGGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGTACCGCACCGAg > 1:2049023/1‑68 (MQ=255)
gcgGGCAGGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGTACCGCACCGAg > 1:2085673/1‑68 (MQ=255)
gcgGGCAGGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGTACCGCACCGAg > 1:2432070/1‑68 (MQ=255)
gcgGGCAGGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGTACCGCACCGAg > 1:1017290/1‑68 (MQ=255)
gcgGGCAGGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGTACCGCACCGAg > 1:2815643/1‑68 (MQ=255)
gcgGGCAGGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGTACCGCACCGAg > 1:2905661/1‑68 (MQ=255)
gcgGGCAGGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGTACCGCACCGAg > 1:2913996/1‑68 (MQ=255)
gcgGGCAGGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGTACCGCACCGAg > 1:3086624/1‑68 (MQ=255)
gcgGGCAGGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGTACCGCACCGAg > 1:3367930/1‑68 (MQ=255)
gcgGGCAGGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGTACCGCACCGAg > 1:899463/1‑68 (MQ=255)
gcgGGCAGGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGTACCGCACCGAg > 1:916327/1‑68 (MQ=255)
gcgGGCAGGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGTACCGCACCGAg > 1:133252/1‑68 (MQ=255)
gcgGGCAGGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGTACCGCACCGAg > 1:1242471/1‑68 (MQ=255)
gcgGGCAGGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGTACCGCACCGAg > 1:1043184/1‑68 (MQ=255)
gcgGGCAGGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGTACCGCACCGAg > 1:1461870/1‑68 (MQ=255)
ggCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTa < 1:1288726/69‑1 (MQ=255)
ggCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTa < 1:1049679/69‑1 (MQ=255)
tGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGTACCGCACCGAGGGTAATCGGc > 1:3409575/1‑68 (MQ=255)
tGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGTACCGCACCGAGGGTAATCGGc > 1:1002674/1‑68 (MQ=255)
tGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGTACCGCACCGAGGGTAATCGGc > 1:1186792/1‑68 (MQ=255)
tGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGTACCGCACCGAGGGTAATCGGc > 1:360464/1‑68 (MQ=255)
aCCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGa < 1:2755/69‑1 (MQ=255)
aCCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGa < 1:1798601/69‑1 (MQ=255)
aCCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGa < 1:651918/69‑1 (MQ=255)
aCCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGa < 1:807179/69‑1 (MQ=255)
aCCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGa < 1:3209838/69‑1 (MQ=255)
aCCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGa < 1:2509663/69‑1 (MQ=255)
aCCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGa < 1:3196451/69‑1 (MQ=255)
aCCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGa < 1:2492933/69‑1 (MQ=255)
cAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGACt < 1:105877/69‑1 (MQ=255)
cAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGACt < 1:2614914/69‑1 (MQ=255)
cAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGACt < 1:109800/69‑1 (MQ=255)
cAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGACt < 1:567886/69‑1 (MQ=255)
cAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGACt < 1:647648/69‑1 (MQ=255)
aGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGACt < 1:1411105/68‑1 (MQ=255)
gCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAgtgt < 1:1156966/49‑1 (MQ=255)
gCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGt < 1:3201034/49‑1 (MQ=255)
gCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGt < 1:952259/49‑1 (MQ=255)
gCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGt < 1:3380309/49‑1 (MQ=255)
gCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGt < 1:1602552/49‑1 (MQ=255)
gCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGt < 1:2573449/49‑1 (MQ=255)
gatgatAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTg > 1:196470/1‑69 (MQ=255)
atAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTAcc > 1:789629/1‑69 (MQ=255)
atAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTAcc > 1:3224917/1‑69 (MQ=255)
aaaCACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGt < 1:812738/69‑1 (MQ=255)
aaaCACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGt < 1:448923/69‑1 (MQ=255)
aaaCACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGt < 1:2145824/69‑1 (MQ=255)
aaaCACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGt < 1:2250571/69‑1 (MQ=255)
aaaCACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGt < 1:225535/69‑1 (MQ=255)
aaaCACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGt < 1:2839088/69‑1 (MQ=255)
gAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGa < 1:3175436/69‑1 (MQ=255)
tGGCTATCAGTACCGCACCGAGGGTAATCGGCTCCAGGCTTTc < 1:483355/43‑1 (MQ=25)
tGGCAATCAGTACCGCACCGAGGGTAATCGGCTCCAGGCTTTc < 1:1939040/43‑1 (MQ=37)
tGGCAATCAGTACCGCACCGAGGGTAATCGGCTCCAGGCTTTc < 1:2584699/43‑1 (MQ=37)
tGGCAATCAGTACCGCACCGAGGGTAATCGGCTCCAGGCTTTc < 1:1609684/43‑1 (MQ=37)
tGGCAATCAGTACCGCACCGAGGGTAATCGGCTCCAGGCTTTc < 1:1347142/43‑1 (MQ=37)
tGGCAATCAGTACCGCACCGAGGGTAATCGGCTCCAGGCTTTc < 1:62344/43‑1 (MQ=37)
tGGCAATCAGTACCGCACCGAGGGTAATCGGCTCCAGGCTTTc < 1:50455/43‑1 (MQ=37)
tGGCAATCAGTACCGCACCGAGGGTAATCGGCTCCAGGCTTTc < 1:391969/43‑1 (MQ=37)
tGGCAATCAGTACCGCACCGAGGGTAATCGGCTCCAGGCTTTc < 1:3355190/43‑1 (MQ=37)
tGGCAATCAGTACCGCACCGAGGGTAATCGGCTCCAGGCTTTc < 1:2287889/43‑1 (MQ=37)
tGGCAATCAGTACCGCACCGAGGGTAATCGGCTCCAGGCTTTc < 1:3086932/43‑1 (MQ=37)
cAATCAGTACCGCACCGAGGGTAATCGGCTCCAGGCTTTc < 1:1271763/40‑1 (MQ=37)
cAGCACCGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCcaca > 1:1335482/1‑68 (MQ=255)
cAGCACCGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCcaca > 1:1878512/1‑68 (MQ=255)
cAGCACCGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCcaca > 1:1404143/1‑68 (MQ=255)
cAGCACCGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCcaca > 1:1400146/1‑68 (MQ=255)
cAGCACCGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCcaca > 1:3222526/1‑68 (MQ=255)
cAGCACCGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCcaca > 1:1160019/1‑68 (MQ=255)
cAGCACCGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCcaca > 1:1290977/1‑68 (MQ=255)
cAGCACCGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCcaca > 1:2641474/1‑68 (MQ=255)
aGCACCGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTTACa < 1:1840017/60‑1 (MQ=255)
aGCACCGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACa < 1:1527349/60‑1 (MQ=255)
aGCACCGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACa < 1:897231/60‑1 (MQ=255)
aGCACCGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACa < 1:2338328/60‑1 (MQ=255)
aGCACCGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACa < 1:117581/60‑1 (MQ=255)
aGCACCGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACa < 1:2640119/60‑1 (MQ=255)
aGCACCGCACCGAGGGTAATTGGCTCCAGACTTTCTACACCCTGTACCGTGGAGGTGACa < 1:2784814/60‑1 (MQ=255)
|
TCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACA > minE/2848466‑2848595
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
