Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F1 I0 R1	4577	57.4	3436498	77.7%	2670158	63.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	2,854,683	T→G	52.8%	I349I (ATA→ATC)	yjeS ←	predicted Fe‑S electron transport protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,854,683	0	T	G	52.8%	24.1 / 74.9	76	I349I (ATA→ATC)	yjeS	predicted Fe‑S electron transport protein
Reads supporting (aligned to +/- strand): ref base T (9/27); new base G (9/31); total (18/58)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCGACTATGCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTACGA  >  minE/2854617‑2854743

aaTCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCGACTATGCACGCATTTCGTCTCTCTa                                                             <  1:1865837/68‑1 (MQ=255)

aaTCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCGACTATGCACGCATTTCGTCTCTCTa                                                             <  1:1891284/68‑1 (MQ=255)

aaTCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCGACTATGCACGCATTTCGTCTCTCTa                                                             <  1:794428/68‑1 (MQ=255)

aaTCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCGACTATGCACGCATTTCGTCTCTCTa                                                             <  1:2116875/68‑1 (MQ=255)

aaTCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCGACTATGCACGCATTTCGTCTCTCTa                                                             <  1:1158844/68‑1 (MQ=255)

aaTCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCGACTATGCACGCATTTCGTCTCTCTa                                                             <  1:123378/68‑1 (MQ=255)

aaTCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCGACTATGCACGCATTTCGTCTCTCTa                                                             <  1:2418921/68‑1 (MQ=255)

aaTCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCGACTATGCACGCATTTCGTCTCTCTa                                                             <  1:1603856/68‑1 (MQ=255)

aaTCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCGACTATGCACGCATTTCGTCTCTCTa                                                             <  1:2552945/68‑1 (MQ=255)

 aTCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCGACTATGCACGCATTTCGTCTCTCTa                                                             <  1:1127586/67‑1 (MQ=255)

 aTCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCGACTATGCACGCATTTCGTCTCTCTa                                                             <  1:1586240/67‑1 (MQ=255)

 aTCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCGACTATGCACGCATTTCGTCTCTCTa                                                             <  1:835764/67‑1 (MQ=255)

  tCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCGACTATGCACGCATTTCGTCTCTCTAttt                                                          >  1:777116/1‑69 (MQ=255)

    aCCCGAACCAGACGCTGTTTTTTCGGTAATTGCACTTCGACCACGCACGCATTTCGCCTCTCGATTtgc                                                        >  1:2642364/1‑69 (MQ=255)

    aCCCGAACCAGACGCTGTTTTTTCGGTAATTGCACTTCGACCACGCACGCATTTCGCCTCTCGATTtgc                                                        >  1:2648339/1‑69 (MQ=255)

    aCCCGAACCAGACGCTGTTTTTTCGGTAATTGCACTTCGACCACGCACGCATTTCGCCTCTCGATTtgc                                                        >  1:2472206/1‑69 (MQ=255)

    aCCCGAACCAGACGCTGTTTTTTCGGTAATTGCACTTCGACCACGCACGCATTTCGCCTCTCGATTtgc                                                        >  1:2653249/1‑69 (MQ=255)

    aCCCGAACCAGACGCTGTTTTTTCGGTAATTGCACTTCGACCACGCACGCATTTCGCCTCTCGATTtgc                                                        >  1:2766498/1‑69 (MQ=255)

    aCCCGAACCAGACGCTGTTTTTTCGGTAATTGCACTTCGACCACGCACGCATTTCGCCTCTCGATTtgc                                                        >  1:296791/1‑69 (MQ=255)

    aCCCGAACCAGACGCTGTTTTTTCGGTAATTGCACTTCGACCACGCACGCATTTCGCCTCTCGATTtgc                                                        >  1:2258613/1‑69 (MQ=255)

    aCCCGAACCAGACGCTGTTTTTTCGGTAATTGCACTTCGACCACGCACGCATTTCGCCTCTCGATTtgc                                                        >  1:2568991/1‑69 (MQ=255)

    aCCCGAACCAGACGCTGTTTTTTCGGTAATTGCACTTCGACCACGCACGCATTTCGCCTCTCGATTtgc                                                        >  1:131832/1‑69 (MQ=255)

         aaCCAGACGCTGTTTTTTCGGCAATTGCACTTCGACTATGCACGCATTTCGTCTCTCTa                                                             <  1:2068060/59‑1 (MQ=255)

            cAGACGCTGTTTTTTCGGCAATTGCACTTCGACTATGCACGCATTTCGTCTCTCTATTTGCTGCGCAAt                                                <  1:1848239/69‑1 (MQ=255)

                 gCTGTTTTTTCGGCAATTGCACTTCGACTATGCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGcc                                            >  1:239483/1‑68 (MQ=255)

                             gtaaTTGCACTTCGACCACGCACGCATTTCGCCTCTCGATTTGCTGCGCAATCGCCCACGCTATGTGc                                <  1:2751448/66‑1 (MQ=255)

                             gtaaTTGCACTTCGACCACGCACGCATTTCGCCTCTCGATTTGCTGCGCAATCGCCCACGCTATGTGc                                <  1:1874225/66‑1 (MQ=255)

                             gtaaTTGCACTTCGACCACGCACGCATTTCGCCTCTCGATTTGCTGCGCAATCGCCCACGCTATGTGc                                <  1:2751734/66‑1 (MQ=255)

                             gtaaTTGCACTTCGACCACGCACGCATTTCGCCTCTCGATTTGCTGCGCAATCGCCCACGCTATGTGc                                <  1:2755457/66‑1 (MQ=255)

                             gtaaTTGCACTTCGACCACGCACGCATTTCGCCTCTCGATTTGCTGCGCAATCGCCCACGCTATGTGc                                <  1:2801920/66‑1 (MQ=255)

                             gtaaTTGCACTTCGACCACGCACGCATTTCGCCTCTCGATTTGCTGCGCAATCGCCCACGCTATGTGc                                <  1:2808574/66‑1 (MQ=255)

                             gtaaTTGCACTTCGACCACGCACGCATTTCGCCTCTCGATTTGCTGCGCAATCGCCCACGCTATGTGc                                <  1:2832643/66‑1 (MQ=255)

                             gtaaTTGCACTTCGACCACGCACGCATTTCGCCTCTCGATTTGCTGCGCAATCGCCCACGCTATGTGc                                <  1:3150039/66‑1 (MQ=255)

                             gtaaTTGCACTTCGACCACGCACGCATTTCGCCTCTCGATTTGCTGCGCAATCGCCCACGCTATGTGc                                <  1:3172502/66‑1 (MQ=255)

                             gtaaTTGCACTTCGACCACGCACGCATTTCGCCTCTCGATTTGCTGCGCAATCGCCCACGCTATGTGc                                <  1:3333228/66‑1 (MQ=255)

                             gtaaTTGCACTTCGACCACGCACGCATTTCGCCTCTCGATTTGCTGCGCAATCGCCCACGCTATGTGc                                <  1:3420124/66‑1 (MQ=255)

                             gtaaTTGCACTTCGACCACGCACGCATTTCGCCTCTCGATTTGCTGCGCAATCGCCCACGCTATGTGc                                <  1:760022/66‑1 (MQ=255)

                             gtaaTTGCACTTCGACCACGCACGCATTTCGCCTCTCGATTTGCTGCGCAATCGCCCACGCTATGTGc                                <  1:1145575/66‑1 (MQ=255)

                             gtaaTTGCACTTCGACCACGCACGCATTTCGCCTCTCGATTTGCTGCGCAATCGCCCACGCTATGTGc                                <  1:777811/66‑1 (MQ=255)

                             gtaaTTGCACTTCGACCACGCACGCATTTCGCCTCTCGATTTGCTGCGCAATCGCCCACGCTATGTGc                                <  1:866135/66‑1 (MQ=255)

                             gtaaTTGCACTTCGACCACGCACGCATTTCGCCTCTCGATTTGCTGCGCAATCGCCCACGCTATGTGc                                <  1:978742/66‑1 (MQ=255)

                             gtaaTTGCACTTCGACCACGCACGCATTTCGCCTCTCGATTTGCTGCGCAATCGCCCACGCTATGTGc                                <  1:994408/66‑1 (MQ=255)

                             gtaaTTGCACTTCGACCACGCACGCATTTCGCCTCTCGATTTGCTGCGCAATCGCCCACGCTATGTGc                                <  1:998628/66‑1 (MQ=255)

                             gtaaTTGCACTTCGACCACGCACGCATTTCGCCTCTCGATTTGCTGCGCAATCGCCCACGCTATGTGc                                <  1:1873320/66‑1 (MQ=255)

                             gtaaTTGCACTTCGACCACGCACGCATTTCGCCTCTCGATTTGCTGCGCAATCGCCCACGCTATGTGc                                <  1:1639766/66‑1 (MQ=255)

                             gtaaTTGCACTTCGACCACGCACGCATTTCGCCTCTCGATTTGCTGCGCAATCGCCCACGCTATGTGc                                <  1:2133725/66‑1 (MQ=255)

                             gtaaTTGCACTTCGACCACGCACGCATTTCGCCTCTCGATTTGCTGCGCAATCGCCCACGCTATGTGc                                <  1:1429939/66‑1 (MQ=255)

                             gtaaTTGCACTTCGACCACGCACGCATTTCGCCTCTCGATTTGCTGCGCAATCGCCCACGCTATGTGc                                <  1:2399941/66‑1 (MQ=255)

                             gtaaTTGCACTTCGACCACGCACGCATTTCGCCTCTCGATTTGCTGCGCAATCGCCCACGCTATGTGc                                <  1:1612705/66‑1 (MQ=255)

                             gtaaTTGCACTTCGACCACGCACGCATTTCGCCTCTCGATTTGCTGCGCAATCGCCCACGCTATGTGc                                <  1:1579033/66‑1 (MQ=255)

                             gtaaTTGCACTTCGACCACGCACGCATTTCGCCTCTCGATTTGCTGCGCAATCGCCCACGCTATGTGc                                <  1:2516528/66‑1 (MQ=255)

                             gtaaTTGCACTTCGACCACGCACGCATTTCGCCTCTCGATTTGCTGCGCAATCGCCCACGCTATGTGc                                <  1:194372/66‑1 (MQ=255)

                             gtaaTTGCACTTCGACCACGCACGCATTTCGCCTCTCGATTTGCTGCGCAATCGCCCACGCTATGTGc                                <  1:1074309/66‑1 (MQ=255)

                             gtaaTTGCACTTCGACCACGCACGCATTTCGCCTCTCGATTTGCTGCGCAATCGCCCACGCTATGTGc                                <  1:2598956/66‑1 (MQ=255)

                             gtaaTTGCACTTCGACCACGCACGCATTTCGCCTCTCGATTTGCTGCGCAATCGCCCACGCTATGTGc                                <  1:2095978/66‑1 (MQ=255)

                             gtaaTTGCACTTCGACCACGCACGCATTTCGCCTCTCGATTTGCTGCGCAATCGCCCACGCTATGTGc                                <  1:1919569/66‑1 (MQ=255)

                              cAATTGCACTTCGACTATGCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTc                              <  1:1523506/69‑1 (MQ=255)

                                  tGCACTTCGACTATGCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGc                                       >  1:1139063/1‑56 (MQ=255)

                                  tGCACTTCGACTATGCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGc                                       >  1:779006/1‑56 (MQ=255)

                                      cTTCGACTATGCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCgaga                       <  1:2974026/68‑1 (MQ=255)

                                      cTTCGACTATGCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCgaga                       <  1:773294/68‑1 (MQ=255)

                                             tATGCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTg               <  1:846820/69‑1 (MQ=255)

                                               tGCACGCATTTCTTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCt             >  1:941051/1‑69 (MQ=255)

                                               tGCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGc              >  1:1402848/1‑68 (MQ=255)

                                               tGCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCt             >  1:629692/1‑69 (MQ=255)

                                                 cACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTc            <  1:659666/68‑1 (MQ=255)

                                                 cACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTc            <  1:652520/68‑1 (MQ=255)

                                                 cACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTc            <  1:2272015/68‑1 (MQ=255)

                                                 cACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTc            <  1:3038407/68‑1 (MQ=255)

                                                 cACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTc            <  1:1129183/68‑1 (MQ=255)

                                                 cACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTc            <  1:2646612/68‑1 (MQ=255)

                                                        ttCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCgaga                       <  1:1721678/50‑1 (MQ=255)

                                                         tCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTACg   >  1:359246/1‑69 (MQ=255)

                                                         tCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTACg   >  1:1479686/1‑69 (MQ=255)

                                                           gTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCgaga                       <  1:1538250/47‑1 (MQ=255)

                                                           gTCTCTATATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTACGa  <  1:271564/68‑1 (MQ=255)

AATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCGACTATGCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTACGA  >  minE/2854617‑2854743

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A