Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,879,789 |
G→A |
48.0% |
I79I (ATC→ATT) |
ytfE ← |
predicted regulator of cell morphogenesis and cell wall metabolism |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,879,789 | 0 | G | A | 48.0%
| ‑0.3
/ 41.5
| 50 | I79I (ATC→ATT) | ytfE | predicted regulator of cell morphogenesis and cell wall metabolism |
| Reads supporting (aligned to +/- strand): ref base G (21/5); new base A (12/12); total (33/17) |
| Fisher's exact test for biased strand distribution p-value = 3.59e-02 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.64e-01 |
CGACTTTAGTCGCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGTAGCGCACGATGATATGGTCGATGATTTCTGCCAGCGGGGCGCTACGCCAGTCTTTCTCAATCGGTTGTTCAGCGAGC > minE/2879722‑2879855
|
cGACTTTAGTCGCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGTAGCGCACgatga < 1:2563164/69‑1 (MQ=255)
cGACTTTAGTCGCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGTAGCGCACgatga < 1:250593/69‑1 (MQ=255)
cGACTTTAGTCGCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGTAGCGCACgatga < 1:2456059/69‑1 (MQ=255)
cGACTTTAGTCGCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGTAGCGCACgatga < 1:2922079/69‑1 (MQ=255)
cGACTTTAGTCGCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGTAGCGCACgatga < 1:1340547/69‑1 (MQ=255)
cGACTTTAGTCGCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGTAGCGCACgatga < 1:703820/69‑1 (MQ=255)
cGACTTTAGTCGCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGTAGCGCACgatga < 1:1706051/69‑1 (MQ=255)
gTCGCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGTAGCGCACGATGATATGGTc < 1:1204648/68‑1 (MQ=255)
gTCGCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGTAGCGCACGATGATATGGTc < 1:181041/68‑1 (MQ=255)
gTCGCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGTAGCGCACGATGATATGGTc < 1:1607015/68‑1 (MQ=255)
gTCGCATGCAGAATCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGTAGCGCACGATGATATGGTc < 1:1725413/68‑1 (MQ=255)
gCTTGCAGGATCAGCTCCGGCAGCTGCTCGCGATGGCGATCGTGATAGCGCACAATAATGTGGTCgatg < 1:1497352/69‑1 (MQ=255)
gCTTGCAGGATCAGCTCCGGCAGCTGCTCGCGATGGCGATCGTGATAGCGCACAATAATGTGGTCgatg < 1:1617320/69‑1 (MQ=255)
gCTTGCAGGATCAGCTCCGGCAGCTGCTCGCGATGGCGATCGTGATAGCGCACAATAATGTGGTCgatg < 1:1645400/69‑1 (MQ=255)
gCTTGCAGGATCAGCTCCGGCAGCTGCTCGCGATGGCGATCGTGATAGCGCACAATAATGTGGTCgatg < 1:1438679/69‑1 (MQ=255)
gCTTGCAGGATCAGCTCCGGCAGCTGCTCGCGATGGCGATCGTGATAGCGCACAATAATGTGGTCgatg < 1:2549177/69‑1 (MQ=255)
gCTTGCAGGATCAGCTCCGGCAGCTGCTCGCGATGGCGATCGTGATAGCGCACAATAATGTGGTCgatg < 1:2673742/69‑1 (MQ=255)
gCTTGCAGGATCAGCTCCGGCAGCTGCTCGCGATGGCGATCGTGATAGCGCACAATAATGTGGTCgatg < 1:616340/69‑1 (MQ=255)
gCTTGCAGGATCAGCTCCGGCAGCTGCTCGCGATGGCGATCGTGATAGCGCACAATAATGTGGTCgatg < 1:575671/69‑1 (MQ=255)
gCTTGCAGGATCAGCTCCGGCAGCTGCTCGCGATGGCGATCGTGATAGCGCACAATAATGTGGTCgatg < 1:1329618/69‑1 (MQ=255)
gCTTGCAGGATCAGCTCCGGCAGCTGCTCGCGATGGCGATCGTGATAGCGCACAATAATGTGGTCgatg < 1:3159589/69‑1 (MQ=255)
gCTTGCAGGATCAGCTCCGGCAGCTGCTCGCGATGGCGATCGTGATAGCGCACAATAATGTGGTCgatg < 1:1281608/69‑1 (MQ=255)
gCTTGCAGGATCAGCTCCGGCAGCTGCTCGCGATGGCGATCGTGATAGCGCACAATAATGTGGTCgatg < 1:1299579/69‑1 (MQ=255)
gCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGTAGCGCACGATGATATGGTCgatg > 1:875324/1‑69 (MQ=255)
gCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGTAGCGCACGATGATATGGTCgatg > 1:2886438/1‑69 (MQ=255)
gCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGTAGCGCACGATGATATGGTCgatg > 1:2189597/1‑69 (MQ=255)
gCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGTAGCGCACGATGATATGGTCgatg > 1:1324964/1‑69 (MQ=255)
gCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGTAGCGCACGATGATATGGTCgatg > 1:2950904/1‑69 (MQ=255)
gCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGTAGCGCACGATGATATGGTCgatg > 1:3035726/1‑69 (MQ=255)
gCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGTAGCGCACGATGATATGGTCgatg > 1:3146866/1‑69 (MQ=255)
ttGCAGAATCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGTAGCGCACGATGATATGGTCgatgat < 1:3149656/69‑1 (MQ=255)
tCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGTAGCGCACGATGATATGGTCGATGATTTCTGcc > 1:1527682/1‑68 (MQ=255)
tCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGTAGCGCACGATGATATGGTCGATGATTTCTGCCa > 1:1199181/1‑69 (MQ=255)
tCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGTAGCGCACGATGATATGGTCGATGATTTCTGCCa > 1:826492/1‑69 (MQ=255)
tCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGTAGCGCACGATGATATGGTCAATGATTTCTGCCa > 1:305662/1‑69 (MQ=255)
aGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGTAGCGCACgatg < 1:1043562/45‑1 (MQ=255)
aGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGTAGCGCACgatg < 1:1903781/45‑1 (MQ=255)
aGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGTAGCGCACgatg < 1:2089307/45‑1 (MQ=255)
aGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGTAGCGCACgatg < 1:631589/45‑1 (MQ=255)
aGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGTAGCGCACgatg < 1:3092552/45‑1 (MQ=255)
gCTCCTGCAGTTGCTCGCGGTGACGATCGTGGTAGCGCACgatg < 1:584639/44‑1 (MQ=255)
tGCTCGCGGTGACGATCGTGGTAGCGCACGATGATATGGTCGATGATTTCTGCCAGCGGGGCGCTAc > 1:2548437/1‑67 (MQ=255)
tGCTCGCGGTGACGATCGTGGTAGCGCACGATGATATGGTCGATGATTTCTGCCAGCGGGGCGCTAc > 1:1721792/1‑67 (MQ=255)
tGCTCGCGGTGACGATCGTGGTAGCGCACGATGATATGGTCGATGATTTCTGCCAGCGGGGCGCTAc > 1:444233/1‑67 (MQ=255)
tGCTCGCGGTGACGATCGTGGTAGCGCACGATGATATGGTCGATGATTTCTGCCAGCGGGGCGCTAc > 1:3073388/1‑67 (MQ=255)
cgcgATGGCGATCGTGATAGCGCACAATAATGTGGTCGATGATTTCCGCCAGCGGGGCGCTACGCCAGt > 1:3342438/1‑69 (MQ=255)
cgcgATGGCGATCGTGATAGCGCACAATAATGTGGTCGATGATTTCCGCCAGCGGGGCGCTACGCCAGt > 1:979180/1‑69 (MQ=255)
cgcgATGGCGATCGTGATAGCGCACAATAATGTGGTCGATGATTTCCGCCAGCGGGGCGCTACGCCAGt > 1:1786053/1‑69 (MQ=255)
cgcgATGGCGATCGTGATAGCGCACAATAATGTGGTCGATGATTTCCGCCAGCGGGGCGCTACGCCAGt > 1:1782076/1‑69 (MQ=255)
cgcgATGGCGATCGTGATAGCGCACAATAATGTGGTCGATGATTTCCGCCAGCGGGGCGCTACGCCAGt > 1:733574/1‑69 (MQ=255)
cgcgATGGCGATCGTGATAGCGCACAATAATGTGGTCGATGATTTCCGCCAGCGGGGCGCTACGCCAGt > 1:1410127/1‑69 (MQ=255)
cgcgATGGCGATCGTGATAGCGCACAATAATGTGGTCGATGATTTCCGCCAGCGGGGCGCTACGCCAGt > 1:3308865/1‑69 (MQ=255)
cgcgATGGCGATCGTGATAGCGCACAATAATGTGGTCGATGATTTCCGCCAGCGGGGCGCTACGCCAGt > 1:303441/1‑69 (MQ=255)
cgcgATGGCGATCGTGATAGCGCACAATAATGTGGTCGATGATTTCCGCCAGCGGGGCGCTACGCCAGt > 1:2108688/1‑69 (MQ=255)
cgcgATGGCGATCGTGATAGCGCACAATAATGTGGTCGATGATTTCCGCCAGCGGGGCGCTACGCCAGt > 1:291917/1‑69 (MQ=255)
cgcgATGGCGATCGTGATAGCGCACAATAATGTGGTCGATGATTTCCGCCAGCGGGGCGCTACGCCAGt > 1:2381560/1‑69 (MQ=255)
cgcgATGGCGATCGTGATAGCGCACAATAATGTGGTCGATGATTTCCGCCAGCGGGGCGCTACGCCAGt > 1:2678012/1‑69 (MQ=255)
aTCGTGGTAGCGCACGATGATATGGTCGATGATTTCTGCCAGCGGGGCGCTACGc > 1:2763736/1‑55 (MQ=255)
aTCGTGGTAGCGCACGATGATATGGTCGATGATTTCTGCCAGCGGGGCGCTACGCCAGTCTTTCTCAAt > 1:3395280/1‑69 (MQ=255)
aTCGTGGTAGCGCACGATGATATGGTCGATGATTTCTGCCAGCGGGGCGCTACGCCAGTCTTTCTCAAt > 1:199337/1‑69 (MQ=255)
aTCGTGGTAGCGCACGATGATATGGTCGATGATTTCTGCCAGCGGGGCGCTACGCCAGTCTTTCTCAAt > 1:3081606/1‑69 (MQ=255)
aTCGTGGTAGCGCACGATGATATGGTCGATGATTTCTGCCAGCGGGGCGCTACGCCAGTCTTTCTCAAt > 1:2032793/1‑69 (MQ=255)
cGTGGTAGCGCACGATGATATGGTCGATGATTTCTGCCAGCGGGGCGCTACGCCAGTCTTTCTCAATCg > 1:200412/1‑69 (MQ=255)
atgatATGGTCGATGATTTCTGCCAGCGGGGCGCTACGCCAGTCTTTCTCAATCGGTTGTTCAGCGAGc > 1:739419/1‑69 (MQ=255)
|
CGACTTTAGTCGCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGTAGCGCACGATGATATGGTCGATGATTTCTGCCAGCGGGGCGCTACGCCAGTCTTTCTCAATCGGTTGTTCAGCGAGC > minE/2879722‑2879855
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
