Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F1 I0 R1 4577 57.4 3436498 77.7% 2670158 63.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,881,261 T→C 44.1% H269R (CAC→CGC)  ytfG ← NAD(P)H:quinone oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,881,2610TC44.1% 21.4 / 82.8 102H269R (CAC→CGC) ytfGNAD(P)H:quinone oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (32/25);  new base C (9/36);  total (41/61)
Fisher's exact test for biased strand distribution p-value = 2.46e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.87e-01

Only 100 of 102 total aligned reads displayed.

TTTAATTAACTAGTTATTAACATTAAAAAGATGGCTTACGCTTTCGGCTAACGTTGTCGTTGGGTGGCCAATCAATTTGCTAAGTGTTTTGCTGTCATCAAACAGACCGCCTTTCGATGCGCC  >  minE/2881197‑2881319
                                                                |                                                          
tttAATTAACTAGTTATTAACATTAAAAAGATGGCTTACGCTTTCGGCTAACGTTGTCGTTGGGCGGcc                                                        <  1:2656289/69‑1 (MQ=255)
tttAATTAACTAGTTATTAACATTAAAAAGATGGCTTACGCTTTCGGCTAACGTTGTCGTTGGGCGGcc                                                        <  1:968316/69‑1 (MQ=255)
tttAATTAACTAGTTATTAACATTAAAAAGATGGCTTACGCTTTCGGCTAACGTTGTCGTTGGGCGGcc                                                        <  1:903878/69‑1 (MQ=255)
tttAATTAACTAGTTATTAACATTAAAAAGATGGCTTACGCTTTCGGCTAACGTTGTCGTTGGGCGGcc                                                        <  1:615784/69‑1 (MQ=255)
tttAATTAACTAGTTATTAACATTAAAAAGATGGCTTACGCTTTCGGCTAACGTTGTCGTTGGGCGGcc                                                        <  1:522004/69‑1 (MQ=255)
tttAATTAACTAGTTATTAACATTAAAAAGATGGCTTACGCTTTCGGCTAACGTTGTCGTTGGGCGGcc                                                        <  1:2833104/69‑1 (MQ=255)
tttAATTAACTAGTTATTAACATTAAAAAGATGGCTTACGCTTTCGGCTAACGTTGTCGTTGGGCGGcc                                                        <  1:2559649/69‑1 (MQ=255)
tttAATTAACTAGTTATTAACATTAAAAAGATGGCTTACGCTTTCGGCTAACGTTGTCGTTGGGCGGcc                                                        <  1:2338718/69‑1 (MQ=255)
tttAATTAACTAGTTATTAACATTAAAAAGATGGCTTACGCTTTCGGCTAACGTTGTCGTTGGGCGGcc                                                        <  1:2255080/69‑1 (MQ=255)
tttAATTAACTAGTTATTAACATTAAAAAGATGGCTTACGCTTTCGGCTAACGTTGTCGTTGGGCGGcc                                                        <  1:1521454/69‑1 (MQ=255)
tttAATTAACTAGTTATTAACATTAAAAAGATGGCTTACGCTTTCGGCTAACGTTGTCGTTGGGCGGcc                                                        <  1:1006051/69‑1 (MQ=255)
tttAATTAACTAGTTATTAACATTAAAAAGATGGCTTACGCTTTCGGCTAACGTTGTCGTTGGGCGGcc                                                        <  1:1222409/69‑1 (MQ=255)
tttAATTAACTAGTTATTAACATTAAAAAGATGGCTTACGCTTTCGGCTAACGTTGTCGTTGGGCGGcc                                                        <  1:1180477/69‑1 (MQ=255)
tttAATTAACTAGTTATTAACATTAAAAAGATGGCTTACGCTTTCGGCTAACGTTGTCGTTGGGCGGcc                                                        <  1:1006543/69‑1 (MQ=255)
tttAATTAACTAGTTATTAACATTAAAAAGATGGCTTACGCTTTCGGCTAACGTTGTCGTTGGGCGGcc                                                        <  1:1148144/69‑1 (MQ=255)
 ttaattaaCTAGTTATTAACATTAAAAAGATGGCTTACGCTTTCGGCTAACGTTGTCGTTGGGTGGcc                                                        <  1:2871100/68‑1 (MQ=255)
  taattaaCTAGTTATTAACATTAAAAAGATGGCTTACGCTTTCGGCTAACGTTGTCGTTGGGTGGCca                                                       >  1:1337909/1‑68 (MQ=255)
  taattaaCTAGTTATTAACATTAAAAAGATGGCTTACGCTTTCGGCTAACGTTGTCGTTGGGTGGCca                                                       >  1:2778428/1‑68 (MQ=255)
  taattaaCTAGTTATTAACATTAAAAAGATGGCTTACGCTTTCGGCTAACGTTGTCGTTGGGTGGCca                                                       >  1:2885190/1‑68 (MQ=255)
  taattaaCTAGTTATTAACATTAAAAAGATGGCTTACGCTTTCGGCTAACGTTGTCGTTGGGTGGCca                                                       >  1:3055838/1‑68 (MQ=255)
      taaCTAGTTATTAACATTAAAAAGATGGCTTACGCTTTCGGCTAACGTTGTCGTTGGGTGGCcaatcaa                                                  >  1:2813755/1‑69 (MQ=255)
      taaCTAGTTATTAACATTAAAAAGATGGCTTACGCTTTCGGCTAACGTTGTCGTTGGGTGGCcaatcaa                                                  >  1:2131744/1‑69 (MQ=255)
      taaCTAGTTATTAACATTAAAAAGATGGCTTACGCTTTCGGCTAACGTTGTCGTTGGGTGGCcaatcaa                                                  >  1:1966736/1‑69 (MQ=255)
               attaACATTAAAAAGATGGCTTACGCTTTCGGCTAACGTTGTCGTTGGGTGGCCAATCAATTTGCTAAg                                         >  1:1941419/1‑69 (MQ=255)
               attaACATTAAAAAGATGGCTTACGCTTTCGGCTAACGTTGTCGTTGGGTGGCCAATCAATTTGCTAAg                                         >  1:2963953/1‑69 (MQ=255)
               attaACATTAAAAAGATGGCTTACGCTTTCGGCTAACGTTGTCGTTGGGTGGCCAATCAATTTGCTAAg                                         >  1:2384449/1‑69 (MQ=255)
                  aaCATTAAAAAGATGGCTTACGCTTTCGGCTAACGTTGTCGTTGGGTGGCCAATCAATTTGCTAAgtgt                                      >  1:1964758/1‑69 (MQ=255)
                  aaCATTAAAAAGATGGCTTACGCTTTCGGCTAACGTTGTCGTTGGGTGGCCAATCAATTTGCTAAgtgt                                      >  1:2483372/1‑69 (MQ=255)
                  aaCATTAAAAAGATGGCTTACGCTTTCGGCTAACGTTGTCGTTGGGTGGCCAATCAATTTGCTAAgtgt                                      >  1:1266355/1‑69 (MQ=255)
                  aaCATTAAAAAGATGGCTTACGCTTTCGGCTAACGTTGTCGTTGGGTGGCCAATCAATTTGCTAAgtgt                                      >  1:1539446/1‑69 (MQ=255)
                  aaCATTAAAAAGATGGCTTACGCTTTCGGCTAACGTTGTCGTTGGGTGGCCAATCAATTTGCTAAgtgt                                      >  1:3234526/1‑69 (MQ=255)
                  aaCATTAAAAAGATGGCTTACGCTTTCGGCTAACGTTGTCGTTGGGTGGCCAATCAATTTGCTAAgtgt                                      >  1:3434820/1‑69 (MQ=255)
                       tAAAAAGATGGCTTACGCTTTCGGCTAACGTTGTCGTTGGGTGGCcaatcaa                                                  >  1:2158424/1‑52 (MQ=255)
                       tAAAAAGATGGCTTACGCTTTCGGCTAACGTTGTCGTTGGGTGGCcaatcaa                                                  >  1:3136427/1‑52 (MQ=255)
                       tAAAAAGATGGCTTACGCTTTCGGCTAACGTTGTCGTTGGGTGGCcaatcaa                                                  >  1:2839245/1‑52 (MQ=255)
                       tAAAAAGATGGCTTACGCTTTCGGCTAACGTTGTCGTTGGGTGGCcaatcaa                                                  >  1:943945/1‑52 (MQ=255)
                       tAAAAAGATGGCTTACGCTTTCGGCTAACGTTGTCGTTGGGTGGCcaa                                                      >  1:1510349/1‑48 (MQ=255)
                       tAAAAAGATGGCTTACGCTTTCGGCTAACGTTGTCGTT‑GGTGGCcaatcaa                                                  >  1:3287561/1‑51 (MQ=255)
                        aaaaaGATGGCTTACGCTTTCGGCTAACGTTGTCGTTGGGTGGCCAATCAATTTGCTAAGTGTTTTGCt                                >  1:3283921/1‑69 (MQ=255)
                        aaaaaGATGGCTTACGCTTTCGGCTAACGTTGTCGTTGGGTGGCCAATCAATTTGCTAAGTGTTTTGCt                                >  1:2829744/1‑69 (MQ=255)
                        aaaaaGATGGCTTACGCTTTCGGCTAACGTTGTCGTTGGGTGGCCAATCAATTTGCTAAGTGTTTTGCt                                >  1:967269/1‑69 (MQ=255)
                        aaaaaGATGGCTTACGCTTTCGGCTAACGTTGTCGTTGGGTGGCCAATCAATTTGCTAAGTGTTTTGCt                                <  1:2833647/69‑1 (MQ=255)
                        aaaaaGATGGCTTACGCTTTCGGCTAACGTTGTCGTTGGGTGGCCAATCAATTTGCTAAGTGTTTTGCt                                >  1:1519622/1‑69 (MQ=255)
                        aaaaaGATGGCTTACGCTTTCGGCTAACGTTGTCGTTGGGTGGCCAATCAATTTGCTAAGTGTTTTGCt                                >  1:1240370/1‑69 (MQ=255)
                        aaaaaGATGGCTTACGCTTTCGGCTAACGTTGTCGTTGGGTGGCCAATCAATTTGCTAAGTGTTTTGCt                                <  1:2543927/69‑1 (MQ=255)
                        aaaaaGATGGCTTACGCTTTCGGCTAACGTTGTCGTTGGGTGGCCAATCAATTTGCTAAGTGTTTTGCt                                <  1:2538848/69‑1 (MQ=255)
                        aaaaaGATGGCTTACGCTTTCGGCTAACGTTGTCGTTGGGTGGCCAATCAATTTGCTAAGTGTTTTGCt                                <  1:84750/69‑1 (MQ=255)
                        aaaaaGATGGCTTACGCTTTCGGCTAACGTTGTCGTTGGGTGGCCAATCAATTTGCTAAGTGTTTTGCt                                <  1:2902255/69‑1 (MQ=255)
                        aaaaaGATGGCTTACGCTTTCGGCTAACGTTGTCGTTGGGTGGCCAATCAATTTGCTAAGTGTTTTGCt                                >  1:873588/1‑69 (MQ=255)
                        aaaaaGATGGCTTACGCTTTCGGCTAACGTTGTCGTTGGGTGGCCAATCAATTTGCTAAGTGTTTTGCt                                <  1:1657006/69‑1 (MQ=255)
                        aaaaaGATGGCTTACGCTTTCGGCTAACGTTGTCGTTGGGTGGCCAATCAATTTGCTAAGTGTTTTGCt                                <  1:910367/69‑1 (MQ=255)
                        aaaaaGATGGCTTACGCTTTCGGCTAACGTTGTCGTTGGGTGGCCAATCAATTTGCTAAGTGTTTTGCt                                <  1:2256125/69‑1 (MQ=255)
                        aaaaaGATGGCTTACGCTTTCGGCTAACGTTGTCGTTGGGTGGCCAATCAATTTGCTAAGTGTTTTGCt                                <  1:3115603/69‑1 (MQ=255)
                        aaaaaGATGGCTTACGCTTTCGGCTAACGTTGTCGTTGGGTGGCCAATCAATTTGCTAAGTGTTTTGCt                                <  1:2243208/69‑1 (MQ=255)
                        aaaaaGATGGCTTACGCTTTCGGCTAACGTTGTCGTTGGGTGGCCAATCAATTTACTAAGTGTTTTGCt                                <  1:1423000/69‑1 (MQ=255)
                        aaaaaGATGGCTTACGCGTTCGGCTAACGTTGTCGTTGGGTGGCCAATCAATTTGCTAAGTGTTTTGCt                                >  1:639236/1‑69 (MQ=255)
                           aaGATGGCTTACGCTTTCGGCTAACGTTGTCGTTGGGTGGCCAATCAATTTGCTAAGTGTTTTGCTGt                              <  1:3177228/68‑1 (MQ=255)
                           aaGATGGCTTACGCTTTCGGCTAACGTTGTCGTTGGGTGGCCAATCAATTTGCTAAGTGTTTTGCTGt                              <  1:1285777/68‑1 (MQ=255)
                           aaGATGGCTTACGCTTTCGGCTAACGTTGTCGTTGGGTGGCCAATCAATTTGCTAAGTGTTTTGCTGt                              <  1:2426176/68‑1 (MQ=255)
                              aTGGCTTACGCTTTCGGCTAACGTTGACGTTGGGTGGCCAATCAATTTGCTAAGTGTTTTGCTGt                              <  1:3038188/65‑1 (MQ=255)
                               tGGCTTACGCTTTCGGCTAACGTTGTCGTTGGGTGGCCAATCAATTTGCTAAGTGTTTTGCTGtcatca                         >  1:3189002/1‑69 (MQ=255)
                               tGGCTTACGCTTTCGGCTAACGTTGTCGTTGGGTGGCCAATCAATTTGCTAAGTGTTTTGCTGtcatca                         >  1:1327316/1‑69 (MQ=255)
                                 gCTTACGCTTTCGGCTAACGTTGTCGTTGGGTGGCCAATCAATTTGCTAAGTGTTTTGCt                                <  1:2611303/60‑1 (MQ=255)
                                  cTTACGCTTTCGGCTAACGTTGTCGTTGGGCGGcc                                                        <  1:2794839/35‑1 (MQ=255)
                                   ttACGCTTTCGGCTAACGTTGTCGTTGGGTGGCCAATCAATTTGCTAAGTGTTTTGCTGTCATCAAACa                     <  1:557123/69‑1 (MQ=255)
                                   ttACGCTTTCGGCTAACGTTGTCGTTGGGTGGCCAATCAATTTGCTAAGTGTTTTGCTGTCATCAAACa                     <  1:2101019/69‑1 (MQ=255)
                                       gCTTTCGGCTAACGTTGTCGTTGGGCGGCCAATCAATTTGCTAAGCGTTTTGCTGTCATCAAACAGa                   <  1:2032113/67‑1 (MQ=255)
                                       gCTTTCGGCTAACGTTGTCGTTGGGCGGCCAATCAATTTGCTAAGCGTTTTGCTGTCATCAAACAGa                   <  1:1935271/67‑1 (MQ=255)
                                       gCTTTCGGCTAACGTTGTCGTTGGGCGGCCAATCAATTTGCTAAGCGTTTTGCTGTCATCAAACAGa                   <  1:163399/67‑1 (MQ=255)
                                       gCTTTCGGCTAACGTTGTCGTTGGGCGGCCAATCAATTTGCTAAGCGTTTTGCTGTCATCAAACAGa                   <  1:575424/67‑1 (MQ=255)
                                       gCTTTCGGCTAACGTTGTCGTTGGGCGGCCAATCAATTTGCTAAGCGTTTTGCTGTCATCAAACAGa                   <  1:2145921/67‑1 (MQ=255)
                                       gCTTTCGGCTAACGTTGTCGTTGGGCGGCCAATCAATTTGCTAAGCGTTTTGCTGTCATCAAACAGa                   <  1:952473/67‑1 (MQ=255)
                                       gCTTTCGGCTAACGTTGTCGTTGGGCGGCCAATCAATTTGCTAAGCGTTTTGCTGTCATCAAACAGa                   <  1:2205630/67‑1 (MQ=255)
                                       gCTTTCGGCTAACGTTGTCGTTGGGCGGCCAATCAATTTGCTAAGCGTTTTGCTGTCATCAAACAGa                   <  1:3247463/67‑1 (MQ=255)
                                       gCTTTCGGCTAACGTTGTCGTTGGGCGGCCAATCAATTTGCTAAGCGTTTTGCTGTCATCAAACAGa                   <  1:2825308/67‑1 (MQ=255)
                                       gCTTTCGGCTAACGTTGTCGTTGGGCGGCCAATCAATTTGCTAAGCGTTTTGCTGTCATCAAACAGa                   <  1:1435135/67‑1 (MQ=255)
                                       gCTTTCGGCTAACGTTGTCGTTGGGCGGCCAATCAATTTGCTAAGCGTTTTGCTGTCATCAAACAGa                   <  1:2206490/67‑1 (MQ=255)
                                       gCTTTCGGCTAACGTTGTCGTTGGGCGGCCAATCAATTTGCTAAGCGTTTTGCTGTCATCAAACAGa                   <  1:2957210/67‑1 (MQ=255)
                                       gCTTTCGGCTAACGTTGTCGTTGGGCGGCCAATCAATTTGCTAAGCGTTTTGCTGTCATCAAACAGa                   <  1:2716951/67‑1 (MQ=255)
                                       gCTTTCGGCTAACGTTGTCGTTGGGCGGCCAATCAATTTGCTAAGCGTTTTGCTGTCATCAAACAGa                   <  1:1006005/67‑1 (MQ=255)
                                       gCTTTCGGCTAACGTTGTCGTTGGGCGGCCAATCAATTTGCTAAGCGTTTTGCTGTCATCAAACAGa                   <  1:2601532/67‑1 (MQ=255)
                                       gCTTTCGGCTAACGTTGTCGTTGGGCGGCCAATCAATTTGCTAAGCGTTTTGCTGTCATCAAACAGa                   <  1:229318/67‑1 (MQ=255)
                                       gCTTTCGGCTAACGTTGTCGTTGGGCGGCCAATCAATTTGCTAAGCGTTTTGCTGTCATCAAACAGa                   <  1:3059897/67‑1 (MQ=255)
                                       gCTTTCGGCTAACGTTGTCGTTGGGCGGCCAATCAATTTGCTAAGCGTTTTGCTGTCATCAAACAGa                   <  1:3062822/67‑1 (MQ=255)
                                       gCTTTCGGCTAACGTTCTCGTTGGGCGGCCAATCAATTTGCTAAGCGTTTTGCTGTCATCAAACAGa                   <  1:3243289/67‑1 (MQ=255)
                                                      tGTCGTTGGGTGGCCAATCAATTTGCTAAGTGTTTTGCTGTCATCAAACAGACCGCCTTTCGATGCGcc  <  1:568035/69‑1 (MQ=255)
                                                      tGTCGTTGGGTGGCCAATCAATTTGCTAAGTGTTTTGCTGTCATCAAACAGACCGCCTTTCGATGCGcc  <  1:756782/69‑1 (MQ=255)
                                                      tGTCGTTGGGTGGCCAATCAATTTGCTAAGTGTTTTGCTGTCATCAAACAGACCGCCTTTCGATGCGcc  <  1:289223/69‑1 (MQ=255)
                                                      tGTCGTTGGGTGGCCAATCAATTTGCTAAGTGTTTTGCTGTCATCAAACAGACCGCCTTTCGATGCGcc  <  1:1776959/69‑1 (MQ=255)
                                                      tGTCGTTGGGTGGCCAATCAATTTGCTAAGTGTTTTGCTGTCATCAAACAGACCGCCTTTCGATGCGcc  <  1:3138485/69‑1 (MQ=255)
                                                      tGTCGTTGGGCGGCCAATCAATTTGCTAAGCGTTTTGCTGTCATCAAACAGACCGCCTTTCGATGCGcc  >  1:2103427/1‑69 (MQ=255)
                                                      tGTCGTTGGGCGGCCAATCAATTTGCTAAGCGTTTTGCTGTCATCAAACAGACCGCCTTTCGATGCGcc  >  1:3294191/1‑69 (MQ=255)
                                                      tGTCGTTGGGCGGCCAATCAATTTGCTAAGCGTTTTGCTGTCATCAAACAGACCGCCTTTCGATGCGcc  >  1:3097512/1‑69 (MQ=255)
                                                      tGTCGTTGGGCGGCCAATCAATTTGCTAAGCGTTTTGCTGTCATCAAACAGACCGCCTTTCGATGCGcc  >  1:3194786/1‑69 (MQ=255)
                                                      tGTCGTTGGGCGGCCAATCAATTTGCTAAGCGTTTTGCTGTCATCAAACAGACCGCCTTTCGATGCGcc  >  1:2454034/1‑69 (MQ=255)
                                                      tGTCGTTGGGCGGCCAATCAATTTGCTAAGCGTTTTGCTGTCATCAAACAGACCGCCTTTCGATGCGcc  >  1:1848569/1‑69 (MQ=255)
                                                      tGTCGTTGGGCGGCCAATCAATTTGCTAAGCGTTTTGCTGTCATCAAACAGACCGCCTTTCGATGCGcc  >  1:2507691/1‑69 (MQ=255)
                                                      tGTCGTTGGGCGGCCAATCAATTTGCTAAGCGTTTTGCTGTCATCAAACAGACCGCCTTTCGATGCGcc  >  1:2224804/1‑69 (MQ=255)
                                                      tGTCGTTGGGCGGCCAATCAATTTGCTAAGCGTTTTGCTGTCATCAAACAGACCGCCTTTCGATGCGcc  >  1:1441300/1‑69 (MQ=255)
                                                             gggCGGCCAATCAATTTGCTAAGCGTTTTGCTGTCATCAAACAGa                   <  1:949319/45‑1 (MQ=255)
                                                                |                                                          
TTTAATTAACTAGTTATTAACATTAAAAAGATGGCTTACGCTTTCGGCTAACGTTGTCGTTGGGTGGCCAATCAATTTGCTAAGTGTTTTGCTGTCATCAAACAGACCGCCTTTCGATGCGCC  >  minE/2881197‑2881319

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A