Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,906,404 |
T→A |
39.3% |
A387A (GCA→GCT) |
treB ← |
fused trehalose(maltose)‑specific enzyme IIBC component of PTS |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,906,404 | 0 | T | A | 39.3%
| 72.8
/ 111.2
| 117 | A387A (GCA→GCT) | treB | fused trehalose(maltose)‑specific enzyme IIBC component of PTS |
| Reads supporting (aligned to +/- strand): ref base T (33/38); new base A (23/23); total (56/61) |
| Fisher's exact test for biased strand distribution p-value = 8.50e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.57e-01 |
Only 100 of 117 total aligned reads displayed.
ACCAGAACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGGAGATAGCGGCAGGCACGGAGATCTCGCGTTCATTGTG > minE/2906344‑2906469
|
accagaACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTc < 1:471320/69‑1 (MQ=255)
accagaACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTc < 1:3187436/69‑1 (MQ=255)
accagaACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTc < 1:1232862/69‑1 (MQ=255)
ccagaACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTc < 1:2973255/68‑1 (MQ=255)
atcGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCaa > 1:3228806/1‑67 (MQ=255)
atcGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCaa > 1:1838635/1‑67 (MQ=255)
atcGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCaa > 1:1956625/1‑67 (MQ=255)
atcGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCaa > 1:666157/1‑67 (MQ=255)
atcGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCaa > 1:1022409/1‑67 (MQ=255)
cgcgCACAGCATCGGGAAGCGATATTTCAGGTTGATGCCGTACATAGCAGGCTCAGTGACTCCAAGcc > 1:1303382/1‑68 (MQ=255)
cgcgCACAGCATCGGGAAGCGATATTTCAGGTTGATGCCGTACATAGCAGGCTCAGTGACTCCAAGCCa > 1:674280/1‑69 (MQ=255)
cgcgCACAGCATCGGGAAGCGATATTTCAGGTTGATGCCGTACATAGCAGGCTCAGTGACTCCAAGCCa > 1:2258712/1‑69 (MQ=255)
cgcgCACAGCATCGGGAAGCGATATTTCAGGTTGATGCCGTACATAGCAGGCTCAGTGACTCCAAGCCa > 1:2317671/1‑69 (MQ=255)
cgcgCACAGCATCGGGAAGCGATATTTCAGGTTGATGCCGTACATAGCAGGCTCAGTGACTCCAAGCCa > 1:328803/1‑69 (MQ=255)
cgcgCACAGCATCGGGAAGCGATATTTCAGGTTGATGCCGTACATAGCAGGCTCAGTGACTCCAAGCCa > 1:1568588/1‑69 (MQ=255)
cgcgCACAGCATCGGGAAGCGATATTTCAGGTTGATGCCGTACATAGCAGGCTCAGTGACTCCAAGCCa > 1:457436/1‑69 (MQ=255)
cgcgCACAGCATCGGGAAGAGATATTTCAGGTTGATGCCGTACATAGCAGGCTCAGTGACTCCAAGCCa > 1:1019005/1‑69 (MQ=255)
cacaGCATCGGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCg < 1:1531533/69‑1 (MQ=255)
cacaGCATCGGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCg < 1:2362312/69‑1 (MQ=255)
acaGCATCGGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCg < 1:8956/68‑1 (MQ=255)
caGCATCGGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGga < 1:3215992/69‑1 (MQ=255)
caGCATCGGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGga < 1:1287043/69‑1 (MQ=255)
aGCATCGGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGga < 1:874449/68‑1 (MQ=255)
gCATCGGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGga > 1:2778165/1‑67 (MQ=255)
gCATCGGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGga > 1:3297178/1‑67 (MQ=255)
cATCGGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGgaga > 1:603789/1‑68 (MQ=255)
cATCGGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGGAGAt > 1:2621181/1‑69 (MQ=255)
cATCGGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGGAGAt > 1:1772158/1‑69 (MQ=255)
cATCGGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGGAGAt > 1:1437456/1‑69 (MQ=255)
cATCGGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGGAGAt < 1:10319/69‑1 (MQ=255)
aTCGGGAAGCGATATTTCAGGTTGATGCCGTACATAGCAGGCTCAGTGACTCCAAGCCAGGCGGAGATa > 1:402579/1‑69 (MQ=255)
aTCGGGAAGCGATATTTCAGGTTGATGCCGTACATAGCAGGCTCAGTGACTCCAAGCCAGGCGGAGATa > 1:2466910/1‑69 (MQ=255)
aTCGGGAAGCGATATTTCAGGTTGATGCCGTACATAGCAGGCTCAGTGACTCCAAGCCAGGCGGAGATa > 1:2320863/1‑69 (MQ=255)
aTCGGGAAGCGATATTTCAGGTTGATGCCGTACATAGCAGGCTCAGTGACTCCAAGCCAGGCGGAGATa > 1:2911731/1‑69 (MQ=255)
aTCGGGAAGCGATATTTCAGGTTGATGCCGTACATAGCAGGCTCAGTGACTCCAAGCCAGGCGGAGATa > 1:2266500/1‑69 (MQ=255)
aTCGGGAAGCGATATTTCAGGTTGATGCCGTACATAGCAGGCTCAGTGACTCCAAGCCAGGCGGAGATa > 1:1442231/1‑69 (MQ=255)
aTCGGGAAGCGATATTTCAGGTTGATGCCGTACATAGCAGGCTCAGTGACTCCAAGCCAGGCGGAGATa > 1:3143102/1‑69 (MQ=255)
aTCGGGAAGCGATATTTCAGGTTGATGCCGTACATAGCAGGCTCAGTGACTCCAAGCCAGGCGGAGATa > 1:849153/1‑69 (MQ=255)
aTCGGGAAGCGATATTTCAGGTTGATGCCGTACATAGCAGGCTCAGTGACTCCAAGCCAGGCGGAGATa > 1:404390/1‑69 (MQ=255)
aTCGGGAAGCGATATTTCAGGTTGATGCCGTACATAGCAGGCTCAGTGACTCCAAGCCAGGCGGAGATa > 1:555006/1‑69 (MQ=255)
aTCGGGAAGCGATATTTCAGGTTGATGCCGTACATAGCAGGCTCAGTGACTCCAAGCCAGGCGGAGATa > 1:1762985/1‑69 (MQ=255)
aTCGGGAAGCGATATTTCAGGTTGATGCCGTACATAGCAGGCTCAGTGACTCCAAGCCAGGCGGAGATa > 1:1717678/1‑69 (MQ=255)
gggAAGCGATATTTCAGGTTGATGCCGTACATTGc > 1:546982/1‑35 (MQ=255)
gggAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGc > 1:3005774/1‑54 (MQ=255)
gggAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGc < 1:534425/54‑1 (MQ=255)
gggAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGc < 1:3264554/54‑1 (MQ=255)
gggAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGc > 1:3277111/1‑54 (MQ=255)
gggAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGc > 1:2845550/1‑54 (MQ=255)
gggAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGc > 1:2794604/1‑54 (MQ=255)
gggAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGc < 1:3418075/54‑1 (MQ=255)
gggAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGc < 1:2657429/54‑1 (MQ=255)
gggAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGc > 1:1718341/1‑54 (MQ=255)
gggAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGc < 1:1372707/54‑1 (MQ=255)
gggAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGc < 1:107721/54‑1 (MQ=255)
gggAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGc > 1:2108906/1‑54 (MQ=255)
gggAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGc < 1:2357954/54‑1 (MQ=255)
gggAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGGAGATAGCg < 1:2184681/69‑1 (MQ=255)
gggAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGGAGATAGCg < 1:1426276/69‑1 (MQ=255)
gggAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGGAGATAGCg < 1:3108588/69‑1 (MQ=255)
gggAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGGAGATAGCg < 1:2209225/69‑1 (MQ=255)
gggAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGGAGATAGCg < 1:966132/69‑1 (MQ=255)
gggAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGGAGATAGCg < 1:2726469/69‑1 (MQ=255)
gggAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGGAGATAGCg < 1:2569471/69‑1 (MQ=255)
atatTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGc < 1:2044144/46‑1 (MQ=255)
tatTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGga < 1:1245686/53‑1 (MQ=255)
tatTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGga < 1:1832175/53‑1 (MQ=255)
tCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGGAGATAGCGGCAGGCACGgag < 1:2936930/68‑1 (MQ=255)
tCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGGAGATAGCGGCAGGCACGgag < 1:2854057/68‑1 (MQ=255)
tCAGGTTGATGCCCTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGGAGATAGCGGCAGGCACGgag < 1:3145955/68‑1 (MQ=255)
gTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGGAGATAGCGGCAGGCACGGAGAtct < 1:1057133/68‑1 (MQ=255)
gTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGGAGATAGCGGCAGGCACGGAGAtct < 1:1053174/68‑1 (MQ=255)
ttGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGGAGATAGCGGCAGGCACGGAGATCTcg > 1:2726201/1‑69 (MQ=255)
ttGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGGAGATAGCGGCAGGCACGGAGATCTcg > 1:1565207/1‑69 (MQ=255)
ttGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGGAGATAGCGGCAGGCACGGAGATCTcg > 1:1750821/1‑69 (MQ=255)
ttGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGGAGATAGCGGCAGGCACGGAGATCTcg > 1:1452155/1‑69 (MQ=255)
ttGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGGAGATAGCGGCAGGCACGGAGATCTcg > 1:2237150/1‑69 (MQ=255)
tGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGGAGATAGCGGCAGGCACGGAGATCTcgc > 1:2981947/1‑69 (MQ=255)
tGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGGAGATAGCGGCAGGCACGGAGATCTcgc > 1:2268195/1‑69 (MQ=255)
tGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGGAGATAGAGGCAGGCACGGAGATCTcgc > 1:1455829/1‑69 (MQ=255)
tACATAGCAGGCTCAGTGACTCCAAGCCAGGCGGAGATAGCGGCAGGCACGGAGATCTCGCGTTCATtg < 1:1449906/69‑1 (MQ=255)
tACATAGCAGGCTCAGTGACTCCAAGCCAGGCGGAGATAGCGGCAGGCACGGAGATCTCGCGTTCATtg < 1:867190/69‑1 (MQ=255)
tACATAGCAGGCTCAGTGACTCCAAGCCAGGCGGAGATAGCGGCAGGCACGGAGATCTCGCGTTCATtg < 1:414113/69‑1 (MQ=255)
tACATAGCAGGCTCAGTGACTCCAAGCCAGGCGGAGATAGCGGCAGGCACGGAGATCTCGCGTTCATtg < 1:1615740/69‑1 (MQ=255)
tACATAGCAGGCTCAGTGACTCCAAGCCAGGCGGAGATAGCGGCAGGCACGGAGATCTCGCGTTCATtg < 1:1786529/69‑1 (MQ=255)
tACATAGCAGGCTCAGTGACTCCAAGCCAGGCGGAGATAGCGGCAGGCACGGAGATCTCGCGTTCATtg < 1:3431678/69‑1 (MQ=255)
tACATAGCAGGCTCAGTGACTCCAAGCCAGGCGGAGATAGCGGCAGGCACGGAGATCTCGCGTTCATtg < 1:3423736/69‑1 (MQ=255)
tACATAGCAGGCTCAGTGACTCCAAGCCAGGCGGAGATAGCGGCAGGCACGGAGATCTCGCGTTCATtg < 1:3421443/69‑1 (MQ=255)
tACATAGCAGGCTCAGTGACTCCAAGCCAGGCGGAGATAGCGGCAGGCACGGAGATCTCGCGTTCATtg < 1:3371411/69‑1 (MQ=255)
tACATAGCAGGCTCAGTGACTCCAAGCCAGGCGGAGATAGCGGCAGGCACGGAGATCTCGCGTTCATtg < 1:3364368/69‑1 (MQ=255)
tACATAGCAGGCTCAGTGACTCCAAGCCAGGCGGAGATAGCGGCAGGCACGGAGATCTCGCGTTCATtg < 1:3347156/69‑1 (MQ=255)
tACATAGCAGGCTCAGTGACTCCAAGCCAGGCGGAGATAGCGGCAGGCACGGAGATCTCGCGTTCATtg < 1:2686109/69‑1 (MQ=255)
tACATAGCAGGCTCAGTGACTCCAAGCCAGGCGGAGATAGCGGCAGGCACGGAGATCTCGCGTTCATtg < 1:1876738/69‑1 (MQ=255)
tACATAGCAGGCTCAGTGACTCCAAGCCAGGCGGAGATAGCGGCAGGCACGGAGATCTCGCGTTCATtg < 1:1990295/69‑1 (MQ=255)
tACATAGCAGGCTCAGTGACTCCAAGCCAGGCGGAGATAGCGGCAGGCACGGAGATCTCGCGTTCATtg < 1:2071896/69‑1 (MQ=255)
tACATAGCAGGCTCAGTGACTCCAAGCCAGGCGGAGATAGCGGCAGGCACGGAGATCTCGCGTTCATtg < 1:3068068/69‑1 (MQ=255)
tACATAGCAGGCTCAGTGACTCCAAGCCAGGCGGAGATAGCGGCAGGCACGGAGATCTCGCGTTCATtg < 1:2963934/69‑1 (MQ=255)
tACATAGCAGGCTCAGTGACTCCAAGCCAGGCGGAGATAGCGGCAGGCACGGAGATCTCGCGTTCATtg < 1:2501151/69‑1 (MQ=255)
tACATAGCAGGCTCAGTGACTCCAAGCCAGGCGGAGATAGCGGCAGGCACGGAGATCTCGCGTTCATtg < 1:2704103/69‑1 (MQ=255)
cATTGCAGGCTCAGTGACCCCAAGCCAGGCGGAGATAGCGGCAGGCACGGAGATCTCGCGTTTATtgtg < 1:440542/69‑1 (MQ=255)
cATTGCAGGCTCAGTGACCCCAAGCCAGGCGGAGATAGCGGCAGGCACGGAGATCTCGCGTTCATtgtg < 1:1378048/69‑1 (MQ=255)
|
ACCAGAACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGGAGATAGCGGCAGGCACGGAGATCTCGCGTTCATTGTG > minE/2906344‑2906469
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
