Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,910,340 |
G→A |
54.9% |
P444P (CCG→CCA) |
mgtA → |
magnesium transporter |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,910,340 | 0 | G | A | 54.9%
| 9.1
/ 57.9
| 71 | P444P (CCG→CCA) | mgtA | magnesium transporter |
| Reads supporting (aligned to +/- strand): ref base G (22/10); new base A (15/24); total (37/34) |
| Fisher's exact test for biased strand distribution p-value = 1.67e-02 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGCCAGTCGTTGGCAAAAAATTGATGAGATTCCGTTTGATTTCGAGCGTCGCCGGATGTCGGTGGTAGTGGCAGAAAATACCGAGCACC > minE/2910272‑2910395
|
cTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGCCAGTCGTTGGCAAAAAATTGATGAGATTCCg > 1:2628139/1‑69 (MQ=255)
cTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGCCAGTCGTTGGCAAAAAATTGATGAGATTCCg > 1:2967982/1‑69 (MQ=255)
cTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGCCAGTCGTTGGCAAAAAATTGATGAGATTCCg > 1:901407/1‑69 (MQ=255)
cTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGCCAGTCGTTGGCAAAAAATTGATGAGATTCCg > 1:1911409/1‑69 (MQ=255)
cTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGCCAGTCGTTGGCAAAAAATTGATGAGATTCCg > 1:185285/1‑69 (MQ=255)
cTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGCCAGTCGTTGGCAAAAAATTGATGAGATTCCg > 1:62049/1‑69 (MQ=255)
aaGAGTCAGCGCGCTCGCTGGCCAGTCGTTGGCAAAAAATTGATGAGATTCCGTTTGATTTCGAGCGTc > 1:907743/1‑69 (MQ=255)
gagTCAGCGCGCTCGCTGGCCAGTCGTTGGCAAAAAATTGATGAGATTCCGTTTGa > 1:104408/1‑56 (MQ=255)
gagTCAGCGCGCTCGCTGGCCAGTCGTTGGCAAAAAATTGATGAGATTCCGTTTGa > 1:3250561/1‑56 (MQ=255)
gagTCAGCGCGCTCGCTGGCCAGTCGTTGGCAAAAAATTGATGAGATTCCGTTTGa > 1:480978/1‑56 (MQ=255)
gagTCAGCGCGCTCGCTGGCCAGTCGTTGGCAAAAAATTGATGAGATTCCGTTTGa > 1:606981/1‑56 (MQ=255)
gagTCAGCGCGCTCGCTGGCCAGTCGTTGGCAAAAAATTGATGAGATTCCGTTTGa > 1:60761/1‑56 (MQ=255)
gagTCAGCGCGCTCGCTGGCCAGTCGTTGGCAAAAAATTGATGAGATTCCGTTTGa > 1:1783693/1‑56 (MQ=255)
gagTCAGCGCGCTCGCTGGCCAGTCGTTGGCAAAAAATTGATGAGATTCCGTTTGa > 1:629842/1‑56 (MQ=255)
gagTCAGCGCGCTCGCTGGCCAGTCGTTGGCAAAAAATTGATGAGATTCCGTTTGa > 1:663465/1‑56 (MQ=255)
gagTCAGCGCGCTCGCTGGCCAGTCGTTGGCAAAAAATTGATGAGATTCAGTTTGa > 1:2829887/1‑56 (MQ=255)
aGCGCGCTCGCTGGCCAGTCGTTGGCAAAAAATTGATGAGATTCCGTTTGa > 1:2179063/1‑51 (MQ=255)
aGCGCGCTCGCTGGCCAGTCGTTGGCAAAAAATTGATGAGATTCCGTTTGATTTCGAGCGTCGCCGGAt > 1:1823875/1‑69 (MQ=255)
aGCGCGCTCGCTGGCCAGTCGTTGGCAAAAAATTGATGAGATTCCGTTTGATTTCGAGCGTCGCCGGAt > 1:2806331/1‑69 (MQ=255)
aGCGCGCTCGCTGGCCAGTCGTTGGCAAAAAATTGATGAGATTCCGTTTGATTTCGAGCGTCGCCGGAt > 1:949883/1‑69 (MQ=255)
aGCGCGCTCGCTGGCCAGTCGTTGGCAAAAAATTGATGAGATTCCGTTTGATTTCGAGCGTCGCCGGAt > 1:940956/1‑69 (MQ=255)
aGCGCGCTCGCTGGCCAGCCGTTGGCAAAAAATTGATGAGATTCCATTTGATTTCGAGCGTCGTCGGAt < 1:2713539/69‑1 (MQ=255)
aGCGCGCTCGCTGGCCAGCCGTTGGCAAAAAATTGATGAGATTCCATTTGATTTCGAGCGTCGTCGGAt < 1:2604718/69‑1 (MQ=255)
aGCGCGCTCGCTGGCCAGCCGTTGGCAAAAAATTGATGAGATTCCATTTGATTTCGAGCGTCGTCGGAt < 1:3053384/69‑1 (MQ=255)
aGCGCGCTCGCTGGCCAGCCGTTGGCAAAAAATTGATGAGATTCCATTTGATTTCGAGCGTCGTCGGAt < 1:355498/69‑1 (MQ=255)
aGCGCGCTCGCTGGCCAGCCGTTGGCAAAAAATTGATGAGATTCCATTTGATTTCGAGCGTCGTCGGAt < 1:1366962/69‑1 (MQ=255)
aGCGCGCTCGCTGGCCAGCCGTTGGCAAAAAATTGATGAGATTCCATTTGATTTCGAGCGTCGTCGGAt < 1:1637166/69‑1 (MQ=255)
gcgcgcTCGCTGGCCAGCCGTTGGCAAAAAATTGATGAGATTCCATTTGATTTCGAGCGTCGTCGGAt < 1:32111/68‑1 (MQ=255)
cgcgcTCGCTGGCCAGCCGTTGGCAAAAAATTGATGAGATTCCATTT‑ATTTCGAGCGTCGTCGGATGTc < 1:1199823/69‑1 (MQ=255)
gcgcTCGCTGGCCAGCCGTTGGCAAAAAATTGATGAGATTCCATTTGATTTCGAGCGTCGTCGGATGTc < 1:3282355/69‑1 (MQ=255)
gcgcTCGCTGGCCAGCCGTTGGCAAAAAATTGATGAGATTCCATTTGATTTCGAGCGTCGTCGGATGTc < 1:3035407/69‑1 (MQ=255)
gcgcTCGCTGGCCAGCCGTTGGCAAAAAATTGATGAGATTCCATTTGATTTCGAGCGTCGTCGGATGTc < 1:283418/69‑1 (MQ=255)
gcgcTCGCTGGCCAGCCGTTGGCAAAAAATTGATGAGATTCCATTTGATTTCGAGCGTCGTCGGATGTc < 1:935915/69‑1 (MQ=255)
gcgcTCGCTGGCCAGCCGTTGGCAAAAAATTGATGAGATTCCATTTGATTTCGAGCGTCGTCGGATGTc < 1:1107283/69‑1 (MQ=255)
gcgcTCGCTGGCCAGCCGTTGGCAAAAAATTGATGAGATTCCATTTGATTTCGAGCGTCGTCGGATGTc < 1:636429/69‑1 (MQ=255)
gcgcTCGCTGGCCAGCCGTTGGCAAAAAATTGATGAGATTCCATTTGATTTCGAGCGTCGTCGGATGTc < 1:2730255/69‑1 (MQ=255)
gcgcTCGCTGGCCAGCCGTTGGCAAAAAATTGATGAGATTCCATTTGATTTCGAGCGTCGTCGGATGTc < 1:2688809/69‑1 (MQ=255)
gcgcTCGCTGGCCAGCCGTTGGCAAAAAATTGATGAGATTCCATTTGATTTCGAGCGTCGTCGGATGTc < 1:1377263/69‑1 (MQ=255)
gcgcTCGCTGGCCAGCCGTTGGCAAAAAATTGATGAGATTCCATTTGATTTCGAGCGTCGTCGGATGTc < 1:1667235/69‑1 (MQ=255)
gcgcTCGCTGGCCAGCCGTTGGCAAAAAATTGATGAGATTCCATTTGATTTCGAGCGTCGTCGGATGTc < 1:2113485/69‑1 (MQ=255)
gcgcTCGCTGGCCAGCCGTTGGCAAAAAATTGATGAGATTCCATTTGATTTCGAGCGTCGTCGGATGTc < 1:2181902/69‑1 (MQ=255)
gcgcTCGCTGGCCAGCCGTTGGCAAAAAATTGATGAGATTCCATTTGATTTCGAGCGTCGTCGGATGTc < 1:2262728/69‑1 (MQ=255)
gcgcTCGCTGGCCAGCCGTTGGCAAAAAATTGATGAGATTCCATTTGATTTCGAGCGTCGTCGGATGTc < 1:2595114/69‑1 (MQ=255)
cGTTGGCAAAAAATTGATGAGATTCCGTTTGATTTCGAGCGTCGCCGGATGTCGGTGGTAGt > 1:3066839/1‑62 (MQ=255)
cGTTGGCAAAAAATTGATGAGATTCCGTTTGATTTCGAGCGTCGCCGGATGTCGGTGGTAGTg > 1:2880474/1‑63 (MQ=255)
cGTTGGCAAAAAATTGATGAGATTCCGTTTGATTTCGAGCGTCGCCGGATGTCGGTGGTAGTg > 1:2910121/1‑63 (MQ=255)
cGTTGGCAAAAAATTGATGAGATTCCGTTTGATTTCGAGCGTCGCCGGATGTCGGTGGTAGTg > 1:1291749/1‑63 (MQ=255)
cGTTGGCAAAAAATTGATGAGATTCCGTTTGATTTCGAGCGTCGCCGGATGTCGGTGGTAGTg > 1:1489081/1‑63 (MQ=255)
cGTTGGCAAAAAATTGATGAGATTCCGTTTGATTTCGAGCGTCGCCGGATGTCGGTGGTAGTg > 1:550551/1‑63 (MQ=255)
cGTTGGCAAAAAATTGATGAGATTCCGTTTGATTTCGAGCGTCGCCGGATGTCGGTGGTAGTg > 1:410001/1‑63 (MQ=255)
tGGCAAAAAATTGATGAGATTCCGTTTGATTTCGAGCGTCGCCGGATGTCGGTGGTAGTGGCAGAAAAt < 1:2561078/69‑1 (MQ=255)
tGGCAAAAAATTGATGAGATTCCGTTTGATTTCGAGCGTCGCCGGATGTCGGTGGTAGTGGCAGAAAAt < 1:904131/69‑1 (MQ=255)
tGGCAAAAAATTGATGAGATTCCGTTTGATTTCGAGCGTCGCCGGATGTCGGTGGTAGTGGCAGAAAAt < 1:889771/69‑1 (MQ=255)
tGGCAAAAAATTGATGAGATTCCGTTTGATTTCGAGCGTCGCCGGATGTCGGTGGTAGTGGCAGAAAAt < 1:2205570/69‑1 (MQ=255)
tGGCAAAAAATTGATGAGATTCCGTTTGATTTCGAGCGTCGCCGGATGTCGGTGGTAGTGGCAGAAAAt < 1:3328332/69‑1 (MQ=255)
tGGCAAAAAATTGATGAGATTCCGTTTGATTTCGAGCGTCGCCGGATGTCGGTGGTAGTGGCAGAAAAt < 1:2385753/69‑1 (MQ=255)
tGGCAAAAAATTGATGAGATTCCGTTTGATTTCGAGCGTCGCCGGATGTCGGTGGTAGTGGCAGAAAAt < 1:2504214/69‑1 (MQ=255)
aaaaaaTTGATGAGATTCCGTTTGATTTCGAGCGTCGCCGGATGTCGGTGGTAGTGGCAGAAAATACCg < 1:824636/69‑1 (MQ=255)
aaaaaaTTGATGAGATTCCGTTTGATTTCGAGCGTCGCCGGATGTCGGTGGTAGTGGCAGAAAATACCg < 1:1639146/69‑1 (MQ=255)
aaaaaaTTGATGAGATTCCATTTGATTTCGAGCGTCGTCGGATGTCggtggt > 1:1128311/1‑52 (MQ=255)
aaaaaaTTGATGAGATTCCATTTGATTTCGAGCGTCGTCGGATGTCggtggt > 1:2380716/1‑52 (MQ=255)
aaaaaaTTGATGAGATTCCATTTGATTTCGAGCGTCGTCGGATGTCggtggt > 1:1155714/1‑52 (MQ=255)
aaaaaaTTGATGAGATTCCATTTGATTTCGAGCGTCGTCGGATGTCggtggt > 1:2937491/1‑52 (MQ=255)
aaaaaaTTGATGAGATTCCATTTGATTTCGAGCGTCGTCGGATGTCggtggt > 1:2694299/1‑52 (MQ=255)
aaaaaaTTGATGAGATTCCATTTGATTTCGAGCGTCGTCGGATGTCggtggt > 1:2962636/1‑52 (MQ=255)
aaaaaaTTGATGAGATTCCATTTGATTTCGAGCGTCGTCGGATGTCggtggt > 1:1465577/1‑52 (MQ=255)
aaaaaaTTGATGAGATTCCATTTGATTTCGAGCGTCGTCGGATGTCggtggt > 1:2991938/1‑52 (MQ=255)
aaaaaaTTGATGAGATTCCATTTGATTTCGAGCGTCGTCGGATGTCggtggt > 1:2720282/1‑52 (MQ=255)
aaaaaaTTGATGAGATTCCATTTGATTTCGAGCGTCGTCGGATGTCggtggt > 1:2143238/1‑52 (MQ=255)
aaaaaaTTGATGAGATTCCATTTGATTTCGAGCGTCGTCGGATGTCggtggt > 1:2381738/1‑52 (MQ=255)
aaaaaaTTGATGAGATTCCATTTGATTTCGAGCGTCGTCGGATGTCggtggt > 1:263906/1‑52 (MQ=255)
aaaaaaTTGATGAGATTCCATTTGATTTCGAGCGTCGTCGGATGTCggtgg > 1:3139104/1‑51 (MQ=255)
aaaaaaTTGATGAGATTCCATTTGATTTCGAGCGTCGTCGGATGTCgg > 1:1610016/1‑48 (MQ=255)
aaaaaaTTGATGAGATTCCATTTGATTT‑‑AGCGTCGTCGGATGTCggtggt > 1:2666274/1‑50 (MQ=255)
aTTGATGAGATTCCATTTGATTTCGAGCGTCGTCGGAt < 1:3171727/38‑1 (MQ=38)
tgatgaGATTCCGTTTGATTTCGAGCGTCGCCGGATGTCGGTGGTAGTGGCAGAAAATACCGAGCAcc < 1:2261261/68‑1 (MQ=255)
gatgaGATTCCATTTGATTTCGAGCGTCGTCGGAt < 1:2741647/35‑1 (MQ=255)
|
CTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGCCAGTCGTTGGCAAAAAATTGATGAGATTCCGTTTGATTTCGAGCGTCGCCGGATGTCGGTGGTAGTGGCAGAAAATACCGAGCACC > minE/2910272‑2910395
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
