Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F1 I0 R1 4577 57.4 3436498 77.7% 2670158 63.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,928,253 T→C 58.7% L218L (TTG→CTG)  yjgP → conserved inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,928,2530TC58.7% 65.3 / 108.9 150L218L (TTG→CTG) yjgPconserved inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (34/28);  new base C (51/37);  total (85/65)
Fisher's exact test for biased strand distribution p-value = 7.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 158 total aligned reads displayed.

TAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCATTGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTG  >  minE/2928185‑2928318
                                                                    |                                                                 
tAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAt                                                                   >  1:1377827/1‑69 (MQ=255)
tAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAt                                                                   >  1:755155/1‑69 (MQ=255)
tAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAt                                                                   >  1:3256550/1‑69 (MQ=255)
tAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGACACTGCAt                                                                   >  1:2987965/1‑69 (MQ=255)
         tGCGCGACGCCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCATTGTTACGTg                                                          <  1:84361/69‑1 (MQ=255)
                     cccAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCACTGTTACGTTATTTCCGCATTa                                              <  1:1547517/69‑1 (MQ=255)
                     cccAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCACTGTTACGTGATTTCCGCATTa                                              <  1:2689905/69‑1 (MQ=255)
                     cccAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCACTGTTACGTGATTTCCGCATTa                                              <  1:3004132/69‑1 (MQ=255)
                     cccAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCACTGTTACGTGATTTCCGCATTa                                              <  1:2881693/69‑1 (MQ=255)
                     cccAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCACTGTTACGTGATTTCCGCATTa                                              <  1:1841453/69‑1 (MQ=255)
                     cccAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCACTGTTACGTGATTTCCGCATTa                                              <  1:2726935/69‑1 (MQ=255)
                     cccAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCACTGTTACGTGATTTCCGCATTa                                              <  1:2367436/69‑1 (MQ=255)
                     cccAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCACTGTTACGTGATTTCCGCATTa                                              <  1:1901888/69‑1 (MQ=255)
                     cccAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCACTGTTACGTGATTTCCGCATTa                                              <  1:2048689/69‑1 (MQ=255)
                     cccAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCACTGTTACGTGATTTCCGCATTa                                              <  1:2631191/69‑1 (MQ=255)
                     cccAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCACTGTTACGTGATTTCCGCATTa                                              <  1:240470/69‑1 (MQ=255)
                     cccAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCACTGTTACGTGATTTCCGCATTa                                              <  1:2401269/69‑1 (MQ=255)
                     cccAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCACTGTTACGTGATTTCCGCATTa                                              <  1:855017/69‑1 (MQ=255)
                     cccAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCACTGTTACGTGATTTCCGCATTa                                              <  1:3252804/69‑1 (MQ=255)
                     cccAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCACTGTTACGTGATTTCCGCATTa                                              <  1:3108836/69‑1 (MQ=255)
                        aGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCATTGTTACGTGATTTGCGCATTACg                                            <  1:3153668/68‑1 (MQ=255)
                        aGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCATTGTTACGTGATTTCCGCATTACgg                                           <  1:175616/69‑1 (MQ=255)
                        aGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCATTGTTACGTGATTTCCGCATTACgg                                           <  1:3251941/69‑1 (MQ=255)
                        aGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCATTGTTACGTGATTTCCGCATTACgg                                           <  1:3411935/69‑1 (MQ=255)
                        aGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCATTGTTACGTGATTTCCGCATTACgg                                           <  1:2586601/69‑1 (MQ=255)
                        aGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCATTGTTACGTGATTTCCGCATTACgg                                           <  1:767524/69‑1 (MQ=255)
                        aGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCATTGTTACGTGATTTCCGCATTACgg                                           <  1:1104137/69‑1 (MQ=255)
                        aGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCATTGTTACGTGATTTCCGCATTACg                                            <  1:2667228/68‑1 (MQ=255)
                            cgtcACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCATTGTTACGTTATTTCCGCATTACGGACt                                        >  1:1063106/1‑68 (MQ=255)
                            cgtcACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCATTGTTACGTGATTTCCGCATTACGGACt                                        >  1:1282367/1‑68 (MQ=255)
                                aCTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCATTGTTACGTGATTTCCGCATTACGGACTTCCAg                                   <  1:1211664/69‑1 (MQ=255)
                                aCTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCATTGTTACGTGATTTCCGCATTACGGACTTCCAg                                   <  1:2030566/69‑1 (MQ=255)
                                aCTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCATTGTTACGTGATTTCCGCATTACGGACTTCCAg                                   <  1:1747245/69‑1 (MQ=255)
                                   ctcAACCAGGGAACGCGCTTCGAAGGCACTGCATTGTTACGTGATTTCCGCATTACGGACTTCCAGGAt                                <  1:2844681/69‑1 (MQ=255)
                                        ccAGGGAACGCGCTTCGAAGGCACTGCACTGTTACGTGATTTCCGCATTACGGATTTCCa                                    >  1:2376978/1‑60 (MQ=255)
                                        ccAGGGAACGCGCTTCGAAGGCACTGCACTGTTACGTGATTTCCGCATTACGGATTTCCAGGATTAt                             >  1:1020749/1‑67 (MQ=255)
                                        ccAGGGAACGCGCTTCGAAGGCACTGCACTGTTACGTGATTTCCGCATTACGGATTTCCAGGATTATCa                           >  1:296372/1‑69 (MQ=255)
                                        ccAGGGAACGCGCTTCGAAGGCACTGCACTGTTACGTGATTTCCGCATTACGGATTTCCAGGATTATCa                           >  1:1817771/1‑69 (MQ=255)
                                        ccAGGGAACGCGCTTCGAAGGCACTGCACTGTTACGTGATTTCCGCATTACGGATTTCCAGGATTATCa                           >  1:2158023/1‑69 (MQ=255)
                                        ccAGGGAACGCGCTTCGAAGGCACTGCACTGTTACGTGATTTCCGCATTACGGATTTCCAGGATTATCa                           >  1:2782598/1‑69 (MQ=255)
                                        ccAGGGAACGCGCTTCGAAGGCACTGCACTGTTACGTGATTTCCGCATTACGGATTTCCAGGATTATCa                           >  1:2761475/1‑69 (MQ=255)
                                        ccAGGGAACGCGCTTCGAAGGCACTGCACTGTTACGTGATTTCCGCATTACGGATTTCCAGGATTATCa                           >  1:687140/1‑69 (MQ=255)
                                        ccAGGGAACGCGCTTCGAAGGCACTGCACTGTTACGTGATTTCCGCATTACGGATTTCCAGGATTATCa                           >  1:1629151/1‑69 (MQ=255)
                                        ccAGGGAACGCGCTTCGAAGGCACTGCACTGTTACGTGATTTCCGCATTACGGATTTCCAGGATTATCa                           >  1:1305856/1‑69 (MQ=255)
                                        ccAGGGAACGCGCTTCGAAGGCACTGCACTGTTACGTGATTTCCGCATTACGGATTTAGAGGATTATCa                           >  1:2811875/1‑69 (MQ=255)
                                              aaCGCGCTTCGAAGGCACTGCATTGTTACGTGAtt                                                       >  1:2538867/1‑35 (MQ=255)
                                              aaCGCGCTTCGAAGGCACTGCATTGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCa                           >  1:3387847/1‑63 (MQ=255)
                                              aaCGCGCTTCGAAGGCACTGCATTGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGAt                     >  1:977225/1‑69 (MQ=255)
                                              aaCGCGCTTCGAAGGCACTGCATTGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGAt                     >  1:2943134/1‑69 (MQ=255)
                                              aaCGCGCTTCGAAGGCACTGCATTGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGAt                     >  1:1430282/1‑69 (MQ=255)
                                              aaCGCGCTTCGAAGGCACTGCATTGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGAt                     >  1:2662091/1‑69 (MQ=255)
                                              aaCGCGCTTCGAAGGCACTGCATTGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGAt                     >  1:752144/1‑69 (MQ=255)
                                              aaCGCGCTTCGAAGGCACTGCATTGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGAt                     >  1:1072498/1‑69 (MQ=255)
                                              aaCGCGCTTCGAAGGCACTGCACTGTTACGTGATTTCCGCATTACGGATTTCCAGGATTATCAGGCGa                      >  1:2433104/1‑68 (MQ=255)
                                              aaCGCGCTTCGAAGGCACTGCACTGTTACGTGATTTCCGCATTACGGATTTCCAGGATTATCAGGCGAt                     >  1:3294262/1‑69 (MQ=255)
                                              aaCGCGCTTCGAAGGCACTGCACTGTTACGTGATTTCCGCATTACGGATTTCCAGGATTATCAGGCGAt                     >  1:3344131/1‑69 (MQ=255)
                                              aaCGCGCTTCGAAGGCACTGCACTGTTACGTGATTTCCGCATTACGGATTTCCAGGATTATCAGGCGAt                     >  1:523670/1‑69 (MQ=255)
                                              aaCGCGCTTCGAAGGCACTGCACTGTTACGTGATTTCCGCATTACGGATTTCCAGGATTATCAGGCGAt                     >  1:1388902/1‑69 (MQ=255)
                                              aaCGCGCTTCGAAGGCACTGCACTGTTACGTGATTTCCGCATTACGGATTTCCAGGATTATCAGGCGAt                     >  1:594761/1‑69 (MQ=255)
                                              aaCGCGCTTCGAAGGCACTGCACTGTTACGTGATTTCCGCATTACGGATTTCCAGGATTATCAGGCGAt                     >  1:2237554/1‑69 (MQ=255)
                                              aaCGCGCTTCGAAGGCACTGCACTGTTACGTGATTTCCGCATTACGGATTTCCAGGATTATCAGGCGAt                     >  1:630493/1‑69 (MQ=255)
                                              aaCGCGCTTCGAAGGCACTGCACTGTTACGTGATTTCCGCATTACGGATTTCCAGGATTATCAGGCGAt                     >  1:173895/1‑69 (MQ=255)
                                              aaCGCGCTTCGAAGGCACTGCACTGTTACGTGATTTCCGCATTACGGATTTCCAGGATTATCAGGCGAt                     >  1:710866/1‑69 (MQ=255)
                                              aaCGCGCTTCGAAGGCACTGCACTGTTACGTGATTTCCGCATTACGGATTTCCAGGATTATCAGGCGAt                     >  1:1162131/1‑69 (MQ=255)
                                              aaCGCGCTTCGAAGGCACTGCACTGTTACGTGATTTCCGCATTACGGATTTCCAGGATTATCAGGCGAt                     >  1:3051428/1‑69 (MQ=255)
                                              aaCGCGCTTCGAAGGCACTGCACTGTTACGTGATTTCCGCATTACGGATTTCCAGGATTATCAGGCGAt                     >  1:2750275/1‑69 (MQ=255)
                                              aaCGCGCTTCGAAGGCACTGCACTGTTACGTGATTTCCGCATTACGGATTTCCAGGATTATCAGGCGAt                     >  1:2737430/1‑69 (MQ=255)
                                              aaCGCGCTTCGAAGGCACTGCACTGTTACGTGATTTCCGCATTACGGATTTCCAGGATTATCAGGCGAt                     >  1:1841482/1‑69 (MQ=255)
                                              aaCGCGCTTCGAAGGCACTGCACTGTTACGTGATTTCCGCATTACGGATTTCCAGGATTATCAGGCGAt                     >  1:2411178/1‑69 (MQ=255)
                                              aaCGCGCTTCGAAGGCACTGCACTGTTACGTGATTTCCGCATTACGGATTTCCAGGATTATCAGGCGAt                     >  1:1783229/1‑69 (MQ=255)
                                              aaCGCGCTTCGAAGGCACTGCACTGTTACGTGATTTCCGCATTACGGATTTCCAGGATTATCAGGCGAt                     >  1:2061272/1‑69 (MQ=255)
                                              aaCGCGCTTCGAAGGCACTGCACTGTTACGTGATTTCCGCATTACGGATTTCCAGGATTATCAGGCGAt                     >  1:2457912/1‑69 (MQ=255)
                                              aaCGCGCTTCGAAGGCACTGCACTGTTACGTGATTTCCGCATTACGGATTTCCAGGATTATCAGGCGAt                     >  1:2199766/1‑69 (MQ=255)
                                              aaCGCGCTTCGAAGGCACTGCACTGTTACGTGATTTCCGCATTACGGATTTCCAGGATTATCAGGCGAt                     >  1:2648081/1‑69 (MQ=255)
                                               aCGCGCTTCGAAGGCACTGCATTGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATc                    <  1:1234938/69‑1 (MQ=255)
                                                   gcTTCGAAGGCACTGCATTGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTg                <  1:752172/69‑1 (MQ=255)
                                                   gcTTCGAAGGCACTGCATTGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTg                <  1:839/69‑1 (MQ=255)
                                                     ttCGAAGGCACTGCATTGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGt              <  1:627744/69‑1 (MQ=255)
                                                      tCGAAGGCACTGCATTGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATGATTGGt              <  1:1202348/68‑1 (MQ=255)
                                                               cTGCACTGTTACGTGATTTCCGCATTACGGATTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCgg    <  1:3313261/69‑1 (MQ=255)
                                                               cTGCACTGTTACGTGATTTCCGCATTACGGATTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCgg    <  1:2204523/69‑1 (MQ=255)
                                                               cTGCACTGTTACGTGATTTCCGCATTACGGATTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCgg    <  1:2637877/69‑1 (MQ=255)
                                                               cTGCACTGTTACGTGATTTCCGCATTACGGATTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCgg    <  1:869248/69‑1 (MQ=255)
                                                               cTGCACTGTTACGTGATTTCCGCATTACGGATTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCgg    <  1:2100801/69‑1 (MQ=255)
                                                               cTGCACTGTTACGTGATTTCCGCATTACGGATTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCgg    <  1:1345777/69‑1 (MQ=255)
                                                               cTGCACTGTTACGTGATTTCCGCATTACGGATTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCgg    <  1:3229095/69‑1 (MQ=255)
                                                               cTGCACTGTTACGTGATTTCCGCATTACGGATTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCgg    <  1:1871463/69‑1 (MQ=255)
                                                               cTGCACTGTTACGTGATTTCCGCATTACGGATTTCCAGGAATATCAGGCGATCATTGGTCACCAGGCgg    <  1:3330668/69‑1 (MQ=255)
                                                                 gCATTGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGt   >  1:703303/1‑68 (MQ=255)
                                                                 gCATTGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTg  >  1:1016396/1‑69 (MQ=255)
                                                                 gCATTGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTg  >  1:774518/1‑69 (MQ=255)
                                                                 gCATTGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTg  >  1:2295181/1‑69 (MQ=255)
                                                                 gCATTGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTg  >  1:2165957/1‑69 (MQ=255)
                                                                 gCATTGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTg  >  1:2487879/1‑69 (MQ=255)
                                                                 gCATTGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTg  >  1:2599046/1‑69 (MQ=255)
                                                                 gCATTGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTg  >  1:371101/1‑69 (MQ=255)
                                                                 gCATTGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTg  >  1:2608995/1‑69 (MQ=255)
                                                                 gCATTGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTg  >  1:1341350/1‑69 (MQ=255)
                                                                 gCATTGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTg  >  1:3119339/1‑69 (MQ=255)
                                                                 gCATTGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATAATTGGTCACCAGGCGGTg  >  1:2091443/1‑69 (MQ=255)
                                                                    |                                                                 
TAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCATTGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTG  >  minE/2928185‑2928318

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A