Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F1 I0 R1 4577 57.4 3436498 77.7% 2670158 63.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,956,162 G→A 59.2% E153E (GAG→GAA)  prfC → peptide chain release factor RF‑3

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,956,1620GA59.2% 47.9 / 91.4 125E153E (GAG→GAA) prfCpeptide chain release factor RF‑3
Reads supporting (aligned to +/- strand):  ref base G (41/10);  new base A (50/24);  total (91/34)
Fisher's exact test for biased strand distribution p-value = 1.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.57e-01

Only 100 of 126 total aligned reads displayed.

TGCGCGACACGCCGATCCTCACCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAGCTGCTCGATGAAGTTGAGAACGAGCTGAAAATCGGCTGTGCGCCGATCACCTGG  >  minE/2956098‑2956216
                                                                |                                                      
tGCGCGACACGCCGATCCTCACCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAgctgc                                                    >  1:2325312/1‑69 (MQ=255)
    cgACACGCCGATCCTCACCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAACTGCTCg                                                 >  1:1438270/1‑68 (MQ=255)
    cgACACGCCGATCCTCACCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAACTGCTCg                                                 >  1:2049366/1‑68 (MQ=255)
    cgACACGCCGATCCTCACCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAACTGCTCg                                                 >  1:180290/1‑68 (MQ=255)
    cgACACGCCGATCCTCACCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAACTGCTCg                                                 >  1:210960/1‑68 (MQ=255)
    cgACACGCCGATCCTCACCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAACTGCTCg                                                 >  1:2164001/1‑68 (MQ=255)
    cgACACGCCGATCCTCACCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAACTGCTCg                                                 >  1:2172805/1‑68 (MQ=255)
    cgACACGCCGATCCTCACCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAACTGCTCg                                                 >  1:348522/1‑68 (MQ=255)
    cgACACGCCGATCCTCACCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAACTGCTCg                                                 >  1:3417336/1‑68 (MQ=255)
    cgACACGCCGATCCTCACCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAACTGCTCg                                                 >  1:2277480/1‑68 (MQ=255)
    cgACACGCCGATCCTCACCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAACTGCTCg                                                 >  1:558473/1‑68 (MQ=255)
    cgACACGCCGATCCTCACCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAACTGCTCg                                                 >  1:2373902/1‑68 (MQ=255)
    cgACACGCCGATCCTCACCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAACTGCTCg                                                 >  1:241642/1‑68 (MQ=255)
    cgACACGCCGATCCTCACCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAACTGCTCg                                                 >  1:2492902/1‑68 (MQ=255)
    cgACACGCCGATCCTCACCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAACTGCTCg                                                 >  1:2593257/1‑68 (MQ=255)
    cgACACGCCGATCCTCACCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAACTGCTCg                                                 >  1:1303186/1‑68 (MQ=255)
    cgACACGCCGATCCTCACCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAACTGCTCg                                                 >  1:3213859/1‑68 (MQ=255)
    cgACACGCCGATCCTCACCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAACTGCTCg                                                 >  1:2729726/1‑68 (MQ=255)
    cgACACGCCGATCCTCACCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAACTGCTCg                                                 >  1:905105/1‑68 (MQ=255)
    cgACACGCCGATCCTCACCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAACTGCTCg                                                 >  1:1215602/1‑68 (MQ=255)
    cgACACGCCGATCCTCACCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAACTGCTCg                                                 >  1:2732642/1‑68 (MQ=255)
    cgACACGCCGATCCTCACCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAACTGCTCg                                                 >  1:2896373/1‑68 (MQ=255)
    cgACACGCCGATCCTCACCTTTATGAACAAACTTGACCGTGATATACGCGACCCGATGGAACTGCTCg                                                 >  1:1127097/1‑68 (MQ=255)
         cGCCGATCCTCACCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAGCTGCTCGATGAAg                                           <  1:99864/69‑1 (MQ=255)
         cGCCGATCCTCACCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAGCTGCTCGATGAAg                                           <  1:2789931/69‑1 (MQ=255)
         cGCCGATCCTCACCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAGCTGCTCGATGAAg                                           <  1:3091262/69‑1 (MQ=255)
              aTCCTCACCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAGCTGCTCGATGAAGTTgag                                      <  1:3029414/69‑1 (MQ=255)
              aTCCTCACCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAGCTGCTCGATGAAGTTgag                                      <  1:925117/69‑1 (MQ=255)
              aTCCTCACCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAGCTGCTCGATGAAGTTgag                                      <  1:111815/69‑1 (MQ=255)
              aTCCTCACCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAACTTCTCGATGAAGTTgag                                      <  1:1460777/69‑1 (MQ=255)
              aTCCTCACCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAACTGCTCGATGAAGTTgag                                      <  1:2580151/69‑1 (MQ=255)
              aTCCTCACCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAACTGCTCGATGAAGTTgag                                      <  1:3091052/69‑1 (MQ=255)
              aTCCTCACCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAACTGCTCGATGAAGTTgag                                      <  1:283176/69‑1 (MQ=255)
              aTCCTCACCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAACTGCTCGATGAAGTTgag                                      <  1:3128402/69‑1 (MQ=255)
              aTCCTCACCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAACTGCTCGATGAAGTTgag                                      <  1:104154/69‑1 (MQ=255)
              aTCCTCACCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAACTGCTCGATGAAGTTgag                                      <  1:3132758/69‑1 (MQ=255)
              aTCCTCACCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAACTGCTCGATGAAGTTgag                                      <  1:1337266/69‑1 (MQ=255)
              aTCCTCACCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAACTGCTCGATGAAGTTgag                                      <  1:1919858/69‑1 (MQ=255)
              aTCCTCACCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAACTGCTCGATGAAGTTgag                                      <  1:3296750/69‑1 (MQ=255)
              aTCCTCACCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAACTGCTCGATGAAGTTgag                                      <  1:2363790/69‑1 (MQ=255)
              aTCCTCACCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAACTGCTCGATGAAGTTgag                                      <  1:165021/69‑1 (MQ=255)
              aTCCTCACCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAACTGCTCGATGAAGTTgag                                      <  1:1808727/69‑1 (MQ=255)
              aTCCTCACCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAACTGCTCGATGAAGTTgag                                      <  1:3406171/69‑1 (MQ=255)
              aTCCTCACCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAACTGCTCGATGAAGTTgag                                      <  1:1710838/69‑1 (MQ=255)
              aTCCTCACCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAACTGCTCGATGAAGTTgag                                      <  1:406449/69‑1 (MQ=255)
              aTCCTCACCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAACTGCTCGATGAAGTTgag                                      <  1:3313701/69‑1 (MQ=255)
              aTCCTCACCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAACTGCTCGATGAAGTTgag                                      <  1:475584/69‑1 (MQ=255)
                ccTCACCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAGCTGCTCGATGAAGTTgaga                                     >  1:2449340/1‑68 (MQ=255)
                ccTCACCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAGCTGCTCGATGAAGTTgaga                                     >  1:2861569/1‑68 (MQ=255)
                ccTCACCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAGCTGCTCGATGAAGTTgaga                                     >  1:3280861/1‑68 (MQ=255)
                ccTCACCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAGCTGCTCGATGAAGTTgaga                                     >  1:2749038/1‑68 (MQ=255)
                 cTCACCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAACTGCTCGATGAAGTTgag                                      <  1:393032/66‑1 (MQ=255)
                 cTCACCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAACTGCTCGATGAAGTTgag                                      <  1:2525115/66‑1 (MQ=255)
                   cACCTTTATGAACATACTTGACCGTGATATCCGCGACCCGATGGAGCTGCTCGATGAAGTTGAGAACGa                                 >  1:3163758/1‑69 (MQ=255)
                   cACCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAGCTGCTCGATGAAGTTGAGAACGa                                 >  1:1273817/1‑69 (MQ=255)
                   cACCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAGCTGCTCGATGAAGTTGAGAACGa                                 >  1:1787222/1‑69 (MQ=255)
                   cACCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAGCTGCTCGATGAAGTTGAGAACGa                                 >  1:35476/1‑69 (MQ=255)
                   cACCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAGCTGCTCGATGAAGTTGAGAACGa                                 >  1:286468/1‑69 (MQ=255)
                   cACCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAGCTGCTCGATGAAGTTGAGAACGa                                 >  1:1794564/1‑69 (MQ=255)
                   cACCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAGCTGCTCGATGAAGTTGAGAACGa                                 >  1:2973510/1‑69 (MQ=255)
                   cACCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAGCTGCTCGATGAAGTTGAGAACGa                                 >  1:697292/1‑69 (MQ=255)
                   cACCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAGCTGCTCGATGAAGTTGAGAACGa                                 >  1:693712/1‑69 (MQ=255)
                   cACCTTTATGAACAAACTTGACAGAGATATCCGCGACCCGATGGAGCTGCTCGa                                                >  1:2953552/1‑54 (MQ=255)
                     ccTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAACTGCTCGATGAAGTTgag                                      <  1:3090997/62‑1 (MQ=255)
                       tttATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAGCTGCTCGATGAAGTTGAGAACGAGCt                              >  1:2202038/1‑68 (MQ=255)
                       tttATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAGCTGCTCGATGAAGTTGAGAACGAGCt                              >  1:2786965/1‑68 (MQ=255)
                       tttATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAGCTGCTCGATGAAGTTGAGAACGAGCt                              >  1:821044/1‑68 (MQ=255)
                       tttATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAGCTGCTCGATGAAGTTGAGAACGAGCt                              >  1:1683390/1‑68 (MQ=255)
                        ttATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAGCTGCTCGATGAAGTTGAGAACGAGCTg                             >  1:2688263/1‑68 (MQ=255)
                            gAACAAACTTGACCGTGATATCCGCGACCCGATGGAACTGCTCGATGAAGTTGAGAACGAGCTGAAAAt                        >  1:651283/1‑69 (MQ=255)
                            gAACAAACTTGACCGTGATATCCGCGACCCGATGGAACTGCTCGATGAAGTTGAGAACGAGCTGAAAAt                        >  1:822087/1‑69 (MQ=255)
                            gAACAAACTTGACCGTGATATCCGCGACCCGATGGAACTGCTCGATGAAGTTGAGAACGAGCTGAAAAt                        >  1:3426439/1‑69 (MQ=255)
                                aaaCTTGACCGTGATATCCGCGACCCGATGGAACTGCTc                                                  >  1:1410977/1‑39 (MQ=255)
                                aaaCTTGACCGTGATATCCGCGACCCGATGGAACTGCTc                                                  >  1:1596091/1‑39 (MQ=255)
                                aaaCTTGACCGTGATATCCGCGACCCGATGGAACTGCTc                                                  >  1:1752510/1‑39 (MQ=255)
                                aaaCTTGACCGTGATATCCGCGACCCGATGGAACTGCTc                                                  >  1:834777/1‑39 (MQ=255)
                                aaaCTTGACCGTGATATCCGCGACCCGATGGAACTGCTc                                                  >  1:1957584/1‑39 (MQ=255)
                                aaaCTTGACCGTGATATCCGCGACCCGATGGAACTGCTc                                                  >  1:2143746/1‑39 (MQ=255)
                                aaaCTTGACCGTGATATCCGCGACCCGATGGAACTGCTc                                                  >  1:2258769/1‑39 (MQ=255)
                                aaaCTTGACCGTGATATCCGCGACCCGATGGAACTGCTc                                                  >  1:841223/1‑39 (MQ=255)
                                aaaCTTGACCGTGATATCCGCGACCCGATGGAACTGCTc                                                  >  1:3355429/1‑39 (MQ=255)
                                aaaCTTGACCGTGATATCCGCGACCCGATGGAACTGCTc                                                  >  1:2361131/1‑39 (MQ=255)
                                aaaCTTGACCGTGATATCCGCGACCCGATGGAACTGCTc                                                  >  1:257002/1‑39 (MQ=255)
                                aaaCTTGACCGTGATATCCGCGACCCGATGGAACTGCTc                                                  >  1:121876/1‑39 (MQ=255)
                                aaaCTTGACCGTGATATCCGCGACCCGATGGAACTGCTc                                                  >  1:2753484/1‑39 (MQ=255)
                                aaaCTTGACCGTGATATCCGCGACCCGATGGAACTGCTc                                                  >  1:2831452/1‑39 (MQ=255)
                                                  cgcgACCCGATGGAGCTGCTCGATGAAGTTGAGAACGAGCTGAAAATCGGCTGTGCGCCGAt         >  1:2249635/1‑62 (MQ=255)
                                                  cgcgACCCGATGGAGCTGCTCGATGAAGTTGAGAACGAGCTGAAAATCGGCTGTGCGCCGATCACCTgg  >  1:1038206/1‑69 (MQ=255)
                                                  cgcgACCCGATGGAGCTGCTCGATGAAGTTGAGAACGAGCTGAAAATCGGCTGTGCGCCGATCACCTgg  >  1:2904385/1‑69 (MQ=255)
                                                  cgcgACCCGATGGAGCTGCTCGATGAAGTTGAGAACGAGCTGAAAATCGGCTGTGCGCCGATCACCTgg  >  1:1282770/1‑69 (MQ=255)
                                                  cgcgACCCGATGGAGCTGCTCGATGAAGTTGAGAACGAGCTGAAAATCGGCTGTGCGCCGATCACCTgg  >  1:727404/1‑69 (MQ=255)
                                                  cgcgACCCGATGGAGCTGCTCGATGAAGTTGAGAACGAGCTGAAAATCGGCTGTGCGCCGATCACCTgg  >  1:1017378/1‑69 (MQ=255)
                                                  cgcgACCCGATGGAGCTGCTCGATGAAGTTGAGAACGAGCTGAAAATCGGCTGTGCGCCGATCACCTgg  >  1:1473138/1‑69 (MQ=255)
                                                  cgcgACCCGATGGAGCTGCTCGATGAAGTTGAGAACGAGCTGAAAATCGGCTGTGCGCCGATCACCTgg  >  1:622200/1‑69 (MQ=255)
                                                  cgcgACCCGATGGAGCTGCTCGATGAAGTTGAGAACGAGCTGAAAATCGGCTGTGCGCCGATCACCTgg  >  1:450544/1‑69 (MQ=255)
                                                  cgcgACCCGATGGAGCTGCTCGATGAAGTTGAGAACGAGCTGAAAATCGGCTGTGCGCCGATCACCTgg  >  1:1760327/1‑69 (MQ=255)
                                                  cgcgACCCGATGGAGCTGCTCGATGAAGTTGAGAACGAGCTGAAAATCGGCTGTGCGCCGATCACCTgg  >  1:2299347/1‑69 (MQ=255)
                                                  cgcgACCCGATGGAGCTGCTCGATGAAGTTGAGAACGAGCTGAAAATCGGCTGTGCGCCGATCACCTgg  >  1:2600239/1‑69 (MQ=255)
                                                  cgcgACCCGATGGAGCTGCTCGATGAAGTTGAGAACGAGCTGAAAATCGGCTGTGCGCCGATCACCTgg  >  1:1002262/1‑69 (MQ=255)
                                                  cgcgACCCGATGGAGCTGCTCGATGAAGTTGAGAACGAGCTGAAAATCGGCTGTGCGCCGATCACCTg   >  1:2770599/1‑68 (MQ=255)
                                                                |                                                      
TGCGCGACACGCCGATCCTCACCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAGCTGCTCGATGAAGTTGAGAACGAGCTGAAAATCGGCTGTGCGCCGATCACCTGG  >  minE/2956098‑2956216

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A