Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F8 I0 R1
|
82 |
77.9 |
2158605 |
86.8% |
1873669 |
62.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,057,769 |
Δ1 bp |
100% |
coding (992/4617 nt) |
lhr → |
predicted ATP‑dependent helicase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,057,769 | 0 | C | . | 100.0%
| 39.5
/ NA
| 10 | coding (992/4617 nt) | lhr | predicted ATP‑dependent helicase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base . (10/0); total (10/0) |
| Rejected as polymorphism: Frequency below/above cutoff threshold. |
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
CTTTACGCCGCACGCTTACAGCGTTCCCCGTCTATCGCCGTTGATGCGGCCCATTTCGAGTCGACCTC > minE/1057720‑1057787
|
cTTTACGCCGCACGCTTACAGCGTTCCCCGTCTATCGCCGTTGATGCGG‑CCATTTCGAGTCGACCTc > 1:1066396/1‑67 (MQ=255)
cTTTACGCCGCACGCTTACAGCGTTCCCCGTCTATCGCCGTTGATGCGG‑CCATTTCGAGTCGACCTc > 1:1068511/1‑67 (MQ=255)
cTTTACGCCGCACGCTTACAGCGTTCCCCGTCTATCGCCGTTGATGCGG‑CCATTTCGAGTCGACCTc > 1:1119002/1‑67 (MQ=255)
cTTTACGCCGCACGCTTACAGCGTTCCCCGTCTATCGCCGTTGATGCGG‑CCATTTCGAGTCGACCTc > 1:1225871/1‑67 (MQ=255)
cTTTACGCCGCACGCTTACAGCGTTCCCCGTCTATCGCCGTTGATGCGG‑CCATTTCGAGTCGACCTc > 1:1881695/1‑67 (MQ=255)
cTTTACGCCGCACGCTTACAGCGTTCCCCGTCTATCGCCGTTGATGCGG‑CCATTTCGAGTCGACCTc > 1:192854/1‑67 (MQ=255)
cTTTACGCCGCACGCTTACAGCGTTCCCCGTCTATCGCCGTTGATGCGG‑CCATTTCGAGTCGACCTc > 1:2051234/1‑67 (MQ=255)
cTTTACGCCGCACGCTTACAGCGTTCCCCGTCTATCGCCGTTGATGCGG‑CCATTTCGAGTCGACCTc > 1:2086486/1‑67 (MQ=255)
cTTTACGCCGCACGCTTACAGCGTTCCCCGTCTATCGCCGTTGATGCGG‑CCATTTCGAGTCGACCTc > 1:626928/1‑67 (MQ=255)
cTTTACGCCGCACGCTTACAGCGTTCCCCGTCTATCGCCGTTGATGCGG‑CCATTTCGAGTCGACCTc > 1:851252/1‑67 (MQ=255)
|
CTTTACGCCGCACGCTTACAGCGTTCCCCGTCTATCGCCGTTGATGCGGCCCATTTCGAGTCGACCTC > minE/1057720‑1057787
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
