Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F8 I0 R1
|
82 |
77.9 |
2158605 |
86.8% |
1873669 |
62.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,111,527 |
G→A |
100% |
C287Y (TGT→TAT) |
ydiD → |
short chain acyl‑CoA synthetase, anaerobic |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,111,527 | 0 | G | A | 100.0%
| 275.3
/ NA
| 74 | C287Y (TGT→TAT) | ydiD | short chain acyl‑CoA synthetase, anaerobic |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (0/74); total (0/74) |
| Rejected as polymorphism: Frequency below/above cutoff threshold. |
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
TTGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTGTCTCGCGCTGCTTGAGCAGCAGCGTTGCACCTGTATGCTCGGCGCAACGCCGTTTG > minE/1111482‑1111583
|
ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTTagca < 1:31229/64‑1 (MQ=255)
ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca < 1:396183/64‑1 (MQ=255)
ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca < 1:1863089/64‑1 (MQ=255)
ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca < 1:1868881/64‑1 (MQ=255)
ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca < 1:1879787/64‑1 (MQ=255)
ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca < 1:1922353/64‑1 (MQ=255)
ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca < 1:1924862/64‑1 (MQ=255)
ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca < 1:2004253/64‑1 (MQ=255)
ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca < 1:2047295/64‑1 (MQ=255)
ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca < 1:2057719/64‑1 (MQ=255)
ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca < 1:2083148/64‑1 (MQ=255)
ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca < 1:2114738/64‑1 (MQ=255)
ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca < 1:216167/64‑1 (MQ=255)
ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca < 1:264622/64‑1 (MQ=255)
ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca < 1:315513/64‑1 (MQ=255)
ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca < 1:348691/64‑1 (MQ=255)
ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca < 1:1018169/64‑1 (MQ=255)
ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca < 1:403148/64‑1 (MQ=255)
ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca < 1:408906/64‑1 (MQ=255)
ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca < 1:431766/64‑1 (MQ=255)
ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca < 1:452696/64‑1 (MQ=255)
ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca < 1:457625/64‑1 (MQ=255)
ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca < 1:46729/64‑1 (MQ=255)
ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca < 1:472396/64‑1 (MQ=255)
ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca < 1:557053/64‑1 (MQ=255)
ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca < 1:619956/64‑1 (MQ=255)
ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca < 1:701390/64‑1 (MQ=255)
ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca < 1:73532/64‑1 (MQ=255)
ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca < 1:739301/64‑1 (MQ=255)
ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca < 1:749166/64‑1 (MQ=255)
ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca < 1:873604/64‑1 (MQ=255)
ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca < 1:925147/64‑1 (MQ=255)
ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca < 1:1096228/64‑1 (MQ=255)
ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca < 1:1247611/64‑1 (MQ=255)
ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca < 1:1308357/64‑1 (MQ=255)
ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca < 1:1314987/64‑1 (MQ=255)
ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca < 1:1323618/64‑1 (MQ=255)
ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca < 1:132742/64‑1 (MQ=255)
ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca < 1:1365642/64‑1 (MQ=255)
ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca < 1:1383481/64‑1 (MQ=255)
ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca < 1:140104/64‑1 (MQ=255)
ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca < 1:1220288/64‑1 (MQ=255)
ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca < 1:1152348/64‑1 (MQ=255)
ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca < 1:1140463/64‑1 (MQ=255)
ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca < 1:1411631/64‑1 (MQ=255)
ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca < 1:1445662/64‑1 (MQ=255)
ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca < 1:1093331/64‑1 (MQ=255)
ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca < 1:1075945/64‑1 (MQ=255)
ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca < 1:1550037/64‑1 (MQ=255)
ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca < 1:105290/64‑1 (MQ=255)
ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca < 1:1041879/64‑1 (MQ=255)
ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca < 1:1592644/64‑1 (MQ=255)
ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca < 1:159734/64‑1 (MQ=255)
ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca < 1:1616152/64‑1 (MQ=255)
ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca < 1:1007409/64‑1 (MQ=255)
ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca < 1:1766468/64‑1 (MQ=255)
ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca < 1:1775458/64‑1 (MQ=255)
ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTCagca < 1:4130/64‑1 (MQ=255)
ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTAGagca < 1:944171/64‑1 (MQ=255)
ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCATGATGCGTATCTCGCGCTGCTTGagca < 1:15048/64‑1 (MQ=255)
ttGGCGCTCGCAGCGTGTTGGTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca < 1:1403959/64‑1 (MQ=255)
tGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTTCTTGagca < 1:1580631/63‑1 (MQ=255)
tGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca < 1:271020/63‑1 (MQ=255)
tGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca < 1:2100637/63‑1 (MQ=255)
tGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca < 1:722918/63‑1 (MQ=255)
tGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca < 1:203185/63‑1 (MQ=255)
tGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca < 1:1024035/63‑1 (MQ=255)
tGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca < 1:89012/63‑1 (MQ=255)
cGTGTTGTTAGATATTTTCACTCCTGATGGGTATCTGGCGCTGCTTGagca < 1:1224066/51‑1 (MQ=255)
tgttAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca < 1:2139193/46‑1 (MQ=255)
gttAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca < 1:1630011/45‑1 (MQ=255)
aGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca < 1:298546/42‑1 (MQ=255)
ccTGATGCGTATCTCGCGCTGCTTGAGCAGCAGCGTTGCACCTGTATGCTCGGCGCAACGCCGTTTg < 1:84477/67‑1 (MQ=255)
ccTGATGCGTATCTCGCGCTGCTTGAGCAGCAGCGTTGCACCTGTATGCTCGGCGCAACGCCGTTTg < 1:177205/67‑1 (MQ=255)
|
TTGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTGTCTCGCGCTGCTTGAGCAGCAGCGTTGCACCTGTATGCTCGGCGCAACGCCGTTTG > minE/1111482‑1111583
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
