Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F8 I0 R1
|
82 |
77.9 |
2158605 |
86.8% |
1873669 |
62.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,407,773 |
Δ1 bp |
100% |
coding (42/1590 nt) |
yejF → |
fused predicted oligopeptide transporter subunits and ATP‑binding components of ABC superfamily |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,407,773 | 0 | T | . | 100.0%
| 108.6
/ NA
| 25 | coding (42/1590 nt) | yejF | fused predicted oligopeptide transporter subunits and ATP‑binding components of ABC superfamily |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base . (25/0); total (25/0) |
| Rejected as polymorphism: Frequency below/above cutoff threshold. |
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
GCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAA > minE/1407722‑1407789
|
gCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGG‑TTTCGCCATCAGCaaa > 1:1883556/1‑67 (MQ=255)
gCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGG‑TTTCGCCATCAGCaaa > 1:982474/1‑67 (MQ=255)
gCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGG‑TTTCGCCATCAGCaaa > 1:783178/1‑67 (MQ=255)
gCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGG‑TTTCGCCATCAGCaaa > 1:530199/1‑67 (MQ=255)
gCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGG‑TTTCGCCATCAGCaaa > 1:442261/1‑67 (MQ=255)
gCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGG‑TTTCGCCATCAGCaaa > 1:431475/1‑67 (MQ=255)
gCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGG‑TTTCGCCATCAGCaaa > 1:296427/1‑67 (MQ=255)
gCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGG‑TTTCGCCATCAGCaaa > 1:2090346/1‑67 (MQ=255)
gCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGG‑TTTCGCCATCAGCaaa > 1:1976091/1‑67 (MQ=255)
gCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGG‑TTTCGCCATCAGCaaa > 1:1949007/1‑67 (MQ=255)
gCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGG‑TTTCGCCATCAGCaaa > 1:1005886/1‑67 (MQ=255)
gCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGG‑TTTCGCCATCAGCaaa > 1:1817581/1‑67 (MQ=255)
gCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGG‑TTTCGCCATCAGCaaa > 1:1729671/1‑67 (MQ=255)
gCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGG‑TTTCGCCATCAGCaaa > 1:1725387/1‑67 (MQ=255)
gCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGG‑TTTCGCCATCAGCaaa > 1:1711980/1‑67 (MQ=255)
gCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGG‑TTTCGCCATCAGCaaa > 1:1562103/1‑67 (MQ=255)
gCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGG‑TTTCGCCATCAGCaaa > 1:1450923/1‑67 (MQ=255)
gCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGG‑TTTCGCCATCAGCaaa > 1:1290378/1‑67 (MQ=255)
gCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGG‑TTTCGCCATCAGCaaa > 1:1185492/1‑67 (MQ=255)
gCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGG‑TTTCGCCATCAGCaaa > 1:1160837/1‑67 (MQ=255)
gCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGG‑TTTCGCCATCAGCaaa > 1:1028988/1‑67 (MQ=255)
gCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGG‑TTTCGCCATCAGCaaa > 1:1026540/1‑67 (MQ=255)
gCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGG‑TTTCGCCATCAGCaa > 1:1976890/1‑66 (MQ=255)
gCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGG‑TTTCGCCATCAGCaa > 1:385614/1‑66 (MQ=255)
gCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGG‑TTTCGCCATCAGCaa > 1:98214/1‑66 (MQ=255)
|
GCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAA > minE/1407722‑1407789
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
