Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F8 I0 R1
|
82 |
77.9 |
2158605 |
86.8% |
1873669 |
62.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,520,182 |
Δ1 bp |
100% |
coding (707/813 nt) |
truA ← |
pseudouridylate synthase I |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,520,182 | 0 | G | . | 100.0%
| 11.7
/ NA
| 4 | coding (707/813 nt) | truA | pseudouridylate synthase I |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base . (0/4); total (0/4) |
| Rejected as polymorphism: Frequency below/above cutoff threshold. |
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
CCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGC > minE/1520157‑1520224
|
cccTTCCGCTTTTGCCGTTGCTGCC‑CCAGCTTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGc < 1:1883359/67‑1 (MQ=255)
cccTTCCGCTTTTGCCGTTGCTGCC‑CCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGc < 1:1110294/67‑1 (MQ=255)
cccTTCCGCTTTTGCCGTTGCTGCC‑CCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGc < 1:756035/67‑1 (MQ=255)
ccTTCCGCTTTTGCCGTTGCTGCC‑CCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGc < 1:367982/66‑1 (MQ=255)
|
CCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGC > minE/1520157‑1520224
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
