Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F8 I0 R1
|
82 |
77.9 |
2158605 |
86.8% |
1873669 |
62.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,743,517:1 |
+TTG |
100% |
coding (61/255 nt) |
rpsQ → |
30S ribosomal subunit protein S17 |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,743,517 | 1 | . | T | 81.3%
| 226.2
/ 48.9
| 75 | coding (61/255 nt) | rpsQ | 30S ribosomal subunit protein S17 |
| Reads supporting (aligned to +/- strand): ref base . (0/14); new base T (61/0); total (61/14) |
| Fisher's exact test for biased strand distribution p-value = 1.78e-15 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01 |
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
| * | minE | 2,743,517 | 2 | . | T | 81.3%
| 221.8
/ 48.9
| 75 | coding (61/255 nt) | rpsQ | 30S ribosomal subunit protein S17 |
| Reads supporting (aligned to +/- strand): ref base . (0/14); new base T (61/0); total (61/14) |
| Fisher's exact test for biased strand distribution p-value = 1.78e-15 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.07e-01 |
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
| * | minE | 2,743,517 | 3 | . | G | 81.3%
| 234.3
/ 43.9
| 75 | coding (61/255 nt) | rpsQ | 30S ribosomal subunit protein S17 |
| Reads supporting (aligned to +/- strand): ref base . (0/14); new base G (61/0); total (61/14) |
| Fisher's exact test for biased strand distribution p-value = 1.78e-15 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01 |
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
ATAAAATCCGTACTCTGCAAGGTCGCGTTGTTAGCGACAAAATGGAGAAATCCA‑‑‑TTGTTGTTGCTATCGAACGTTTTGTGAAACACCCGATCTACGGTAAATTCATCAAGCGTACGAC > minE/2743464‑2743581
|||
aTAAAATCCGTACTCTGCAAGGTCGCGTTGTTAGCGACAAAATGGAGAAATCCA‑‑‑TTGTTGTTGCTAt < 1:1041644/67‑1 (MQ=255)
aTAAAATCCGTACTCTGCAAGGTCGCGTTGTTAGCGACAAAATGGAGAAATCCA‑‑‑TTGTTGTTGCTAt < 1:1732514/67‑1 (MQ=255)
aTAAAATCCGTACTCTGCAAGGTCGCGTTGTTAGCGACAAAATGGAGAAATCCA‑‑‑TTGTTGTTGCTAt < 1:900719/67‑1 (MQ=255)
aTAAAATCCGTACTCTGCAAGGTCGCGTTGTTAGCGACAAAATGGAGAAATCCA‑‑‑TTGTTGTTGCTAt < 1:1370233/67‑1 (MQ=255)
aTAAAATCCGTACTCTGCAAGGTCGCGTTGTTAGCGACAAAATGGAGAAATCCA‑‑‑TTGTTGTTGCTAt < 1:2040781/67‑1 (MQ=255)
aTAAAATCCGTACTCTGCAAGGTCGCGTTGTTAGCGACAAAATGGAGAAATCCA‑‑‑TTGTTGTTGCTAt < 1:2123027/67‑1 (MQ=255)
aagTCGCGTTGTTAGCGACAAAATGGAGAAATCCATTGTTGTTGTTGCTATCGAACGTTTTGTGaaa > 1:2100938/3‑67 (MQ=255)
aGGTCGCGTTGTTAGCGACGAAATGGAGAAATCCATTGTTGTTGTTGCTATCGAACGTTTTGTGaaa > 1:608992/1‑67 (MQ=255)
aGGTCGCGTTGTTAGCGACAAAATTGAGAAATCCATTGTTGTTGTTGCTATCGAACGTTTTGTGcaa > 1:1137160/1‑67 (MQ=255)
aGGTCGCGTTGTTAGCGACAAAATGGAGAAATCCATTGTTGTTGTTGCTATCGATCGTTTTGTGaaa > 1:216235/1‑67 (MQ=255)
aGGTCGCGTTGTTAGCGACAAAATGGAGAAATCCATTGTTGTTGTTGCTATCGAACGTTTTGTGaaa > 1:1936198/1‑67 (MQ=255)
aGGTCGCGTTGTTAGCGACAAAATGGAGAAATCCATTGTTGTTGTTGCTATCGAACGTTTTGTGaaa > 1:23914/1‑67 (MQ=255)
aGGTCGCGTTGTTAGCGACAAAATGGAGAAATCCATTGTTGTTGTTGCTATCGAACGTTTTGTGaaa > 1:220132/1‑67 (MQ=255)
aGGTCGCGTTGTTAGCGACAAAATGGAGAAATCCATTGTTGTTGTTGCTATCGAACGTTTTGTGaaa > 1:2151458/1‑67 (MQ=255)
aGGTCGCGTTGTTAGCGACAAAATGGAGAAATCCATTGTTGTTGTTGCTATCGAACGTTTTGTGaaa > 1:2105167/1‑67 (MQ=255)
aGGTCGCGTTGTTAGCGACAAAATGGAGAAATCCATTGTTGTTGTTGCTATCGAACGTTTTGTGaaa > 1:518008/1‑67 (MQ=255)
aGGTCGCGTTGTTAGCGACAAAATGGAGAAATCCATTGTTGTTGTTGCTATCGAACGTTTTGTGaaa > 1:1928881/1‑67 (MQ=255)
aGGTCGCGTTGTTAGCGACAAAATGGAGAAATCCATTGTTGTTGTTGCTATCGAACGTTTTGTGaaa > 1:191553/1‑67 (MQ=255)
aGGTCGCGTTGTTAGCGACAAAATGGAGAAATCCATTGTTGTTGTTGCTATCGAACGTTTTGTGaaa > 1:1911306/1‑67 (MQ=255)
aGGTCGCGTTGTTAGCGACAAAATGGAGAAATCCATTGTTGTTGTTGCTATCGAACGTTTTGTGaaa > 1:1881638/1‑67 (MQ=255)
aGGTCGCGTTGTTAGCGACAAAATGGAGAAATCCATTGTTGTTGTTGCTATCGAACGTTTTGTGaaa > 1:1852108/1‑67 (MQ=255)
aGGTCGCGTTGTTAGCGACAAAATGGAGAAATCCATTGTTGTTGTTGCTATCGAACGTTTTGTGaaa > 1:492584/1‑67 (MQ=255)
aGGTCGCGTTGTTAGCGACAAAATGGAGAAATCCATTGTTGTTGTTGCTATCGAACGTTTTGTGaaa > 1:503390/1‑67 (MQ=255)
aGGTCGCGTTGTTAGCGACAAAATGGAGAAATCCATTGTTGTTGTTGCTATCGAACGTTTTGTGaaa > 1:185829/1‑67 (MQ=255)
aGGTCGCGTTGTTAGCGACAAAATGGAGAAATCCATTGTTGTTGTTGCTATCGAACGTTTTGTGaaa > 1:546934/1‑67 (MQ=255)
aGGTCGCGTTGTTAGCGACAAAATGGAGAAATCCATTGTTGTTGTTGCTATCGAACGTTTTGTGaaa > 1:653919/1‑67 (MQ=255)
aGGTCGCGTTGTTAGCGACAAAATGGAGAAATCCATTGTTGTTGTTGCTATCGAACGTTTTGTGaaa > 1:662641/1‑67 (MQ=255)
aGGTCGCGTTGTTAGCGACAAAATGGAGAAATCCATTGTTGTTGTTGCTATCGAACGTTTTGTGaaa > 1:796673/1‑67 (MQ=255)
aGGTCGCGTTGTTAGCGACAAAATGGAGAAATCCATTGTTGTTGTTGCTATCGAACGTTTTGTGaaa > 1:809940/1‑67 (MQ=255)
aGGTCGCGTTGTTAGCGACAAAATGGAGAAATCCATTGTTGTTGTTGCTATCGAACGTTTTGTGaaa > 1:831957/1‑67 (MQ=255)
aGGTCGCGTTGTTAGCGACAAAATGGAGAAATCCATTGTTGTTGTTGCTATCGAACGTTTTGTGaaa > 1:851946/1‑67 (MQ=255)
aGGTCGCGTTGTTAGCGACAAAATGGAGAAATCCATTGTTGTTGTTGCTATCGAACGTTTTGTGaaa > 1:96981/1‑67 (MQ=255)
aGGTCGCGTTGTTAGCGACAAAATGGAGAAATCCATTGTTGTTGTTGCTATCGAACGTTTTGTGaaa > 1:970569/1‑67 (MQ=255)
aGGTCGCGTTGTTAGCGACAAAATGGAGAAATCCATTGTTGTTGTTGCTATCGAACGTTTTGTGaaa > 1:971147/1‑67 (MQ=255)
aGGTCGCGTTGTTAGCGACAAAATGGAGAAATCCATTGTTGTTGTTGCTATCGAACGTTTTGTGaaa > 1:972126/1‑67 (MQ=255)
aGGTCGCGTTGTTAGCGACAAAATGGAGAAATCCATTGTTGTTGTTGCTATCGAACGTTTTGTGaaa > 1:975007/1‑67 (MQ=255)
aGGTCGCGTTGTTAGCGACAAAATGGAGAAATCCATTGTTGTTGTTGCTATCGAACGTTTTGTGaaa > 1:1502058/1‑67 (MQ=255)
aGGTCGCGTTGTTAGCGACAAAATGGAGAAATCCATTGTTGTTGTTGCTATCGAACGTTTTGTGaaa > 1:1120339/1‑67 (MQ=255)
aGGTCGCGTTGTTAGCGACAAAATGGAGAAATCCATTGTTGTTGTTGCTATCGAACGTTTTGTGaaa > 1:1130198/1‑67 (MQ=255)
aGGTCGCGTTGTTAGCGACAAAATGGAGAAATCCATTGTTGTTGTTGCTATCGAACGTTTTGTGaaa > 1:1182326/1‑67 (MQ=255)
aGGTCGCGTTGTTAGCGACAAAATGGAGAAATCCATTGTTGTTGTTGCTATCGAACGTTTTGTGaaa > 1:1201528/1‑67 (MQ=255)
aGGTCGCGTTGTTAGCGACAAAATGGAGAAATCCATTGTTGTTGTTGCTATCGAACGTTTTGTGaaa > 1:1277199/1‑67 (MQ=255)
aGGTCGCGTTGTTAGCGACAAAATGGAGAAATCCATTGTTGTTGTTGCTATCGAACGTTTTGTGaaa > 1:10973/1‑67 (MQ=255)
aGGTCGCGTTGTTAGCGACAAAATGGAGAAATCCATTGTTGTTGTTGCTATCGAACGTTTTGTGaaa > 1:1312412/1‑67 (MQ=255)
aGGTCGCGTTGTTAGCGACAAAATGGAGAAATCCATTGTTGTTGTTGCTATCGAACGTTTTGTGaaa > 1:1337145/1‑67 (MQ=255)
aGGTCGCGTTGTTAGCGACAAAATGGAGAAATCCATTGTTGTTGTTGCTATCGAACGTTTTGTGaaa > 1:1350593/1‑67 (MQ=255)
aGGTCGCGTTGTTAGCGACAAAATGGAGAAATCCATTGTTGTTGTTGCTATCGAACGTTTTGTGaaa > 1:1374279/1‑67 (MQ=255)
aGGTCGCGTTGTTAGCGACAAAATGGAGAAATCCATTGTTGTTGTTGCTATCGAACGTTTTGTGaaa > 1:109045/1‑67 (MQ=255)
aGGTCGCGTTGTTAGCGACAAAATGGAGAAATCCATTGTTGTTGTTGCTATCGAACGTTTTGTGaaa > 1:1384908/1‑67 (MQ=255)
aGGTCGCGTTGTTAGCGACAAAATGGAGAAATCCATTGTTGTTGTTGCTATCGAACGTTTTGTGaaa > 1:1395111/1‑67 (MQ=255)
aGGTCGCGTTGTTAGCGACAAAATGGAGAAATCCATTGTTGTTGTTGCTATCGAACGTTTTGTGaaa > 1:1481199/1‑67 (MQ=255)
aGGTCGCGTTGTTAGCGACAAAATGGAGAAATCCATTGTTGTTGTTGCTATCGAACGTTTTGTGaaa > 1:1059672/1‑67 (MQ=255)
aGGTCGCGTTGTTAGCGACAAAATGGAGAAATCCATTGTTGTTGTTGCTATCGAACGTTTTGTGaaa > 1:180812/1‑67 (MQ=255)
aGGTCGCGTTGTTAGCGACAAAATGGAGAAATCCATTGTTGTTGTTGCTATCGAACGTTTTGTGaaa > 1:1730545/1‑67 (MQ=255)
aGGTCGCGTTGTTAGCGACAAAATGGAGAAATCCATTGTTGTTGTTGCTATCGAACGTTTTGTGaaa > 1:1707221/1‑67 (MQ=255)
aGGTCGCGTTGTTAGCGACAAAATGGAGAAATCCATTGTTGTTGTTGCTATCGAACGTTTTGTGaaa > 1:1706683/1‑67 (MQ=255)
aGGTCGCGTTGTTAGCGACAAAATGGAGAAATCCATTGTTGTTGTTGCTATCGAACGTTTTGTGaaa > 1:1647995/1‑67 (MQ=255)
aGGTCGCGTTGTTAGCGACAAAATGGAGAAATCCATTGTTGTTGTTGCTATCGAACGTTTTGTGaaa > 1:1639830/1‑67 (MQ=255)
aGGTCGCGTTGTTAGCGACAAAATGGAGAAATCCATTGTTGTTGTTGCTATCGAACGTTTTGTGaaa > 1:1560526/1‑67 (MQ=255)
aGGTCGCGTTGTTAGCGACAAAATGGAGAAATCCATTGTTGTTGTTGCTATCGAACGTTTTGTGaaa > 1:1608113/1‑67 (MQ=255)
aGGTCGCGTTGTTAGCGACAAAATGGAGAAATCCATTGTTGTTGTTGCTATCGAACGTTTTGTGaaa > 1:1603455/1‑67 (MQ=255)
aGGTCGCGTTGTTAGCGACAAAATGGAGAAATCCATTGTTGTTGTTGCTATCGAACGTTTTGTGaaa > 1:1602085/1‑67 (MQ=255)
aGGTCGCGTTGTTAGCGACAAAATGGAGAAATCCATTGTTGTTGTTGCTATCGAACGTTTTGTGaaa > 1:1594214/1‑67 (MQ=255)
aGGTCGCGTTGTTAGCGACAAAATGGAGAAATCCATTGTTGTTGTTGCTATCGAACGTTTTGTGaaa > 1:1585350/1‑67 (MQ=255)
aGGTCGCGTTGTTAGCGACAAAATGGAGAAATCCATTGTTGTTGTTGCTATCGAACGTTTTGTGaaa > 1:1569830/1‑67 (MQ=255)
aGGTCGCGTTGTTAGCGACAAAATGGAGAAATCCATTGTTGTTGTTGCTATCGAACGTTTTGTGaa > 1:732458/1‑66 (MQ=255)
aGGTCGCGTTGTTAGCGACAAAATGGAGAAATCCATTGTTGTTGTTGCTATCGAACGTTTTGTGaa > 1:828545/1‑66 (MQ=255)
ccA‑‑‑TTGTTGTTGCTATCGAACGTTTTGTGAAACACCCGATCTACGGTAAATTCATCAAGCGTACGAc < 1:1082645/67‑1 (MQ=255)
ccA‑‑‑TTGTTGTTGCTATCGAACGTTTTGTGAAACACCCGATCTACGGTAAATTCATCAAGCGTACGAc < 1:101772/67‑1 (MQ=255)
ccA‑‑‑TTGTTGTTGCTATCGAACGTTTTGTGAAACACCCGATCTACGGTAAATTCATCAAGCGTACGAc < 1:92148/67‑1 (MQ=255)
ccA‑‑‑TTGTTGTTGCTATCGAACGTTTTGTGAAACACCCGATCTACGGTAAATTCATCAAGCGTACGAc < 1:746645/67‑1 (MQ=255)
ccA‑‑‑TTGTTGTTGCTATCGAACGTTTTGTGAAACACCCGATCTACGGTAAATTCATCAAGCGTACGAc < 1:543138/67‑1 (MQ=255)
ccA‑‑‑TTGTTGTTGCTATCGAACGTTTTGTGAAACACCCGATCTACGGTAAATTCATCAAGCGTACGAc < 1:2039938/67‑1 (MQ=255)
ccA‑‑‑TTGTTGTTGCTATCGAACGTTTTGTGAAACACCCGATCTACGGTAAATTCATCAAGCGTACGAc < 1:2014831/67‑1 (MQ=255)
ccA‑‑‑TTGTTGTTGCTATCGAACGTTTTGTGAAACACCCGATCTACGGTAAATTCATCAAGCGTACGAc < 1:1001083/67‑1 (MQ=255)
|||
ATAAAATCCGTACTCTGCAAGGTCGCGTTGTTAGCGACAAAATGGAGAAATCCA‑‑‑TTGTTGTTGCTATCGAACGTTTTGTGAAACACCCGATCTACGGTAAATTCATCAAGCGTACGAC > minE/2743464‑2743581
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
