Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F50 I0 R1	26	138.6	6843716	94.5%	6467311	99.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	757,838	Δ1 bp	24.5%	coding (150/717 nt)	sdhB →	succinate dehydrogenase, FeS subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	757,838	0	G	.	24.5%	283.8 / 101.3	106	coding (150/717 nt)	sdhB	succinate dehydrogenase, FeS subunit
Reads supporting (aligned to +/- strand): ref base G (34/46); new base . (13/13); total (47/59)
Fisher's exact test for biased strand distribution p-value = 6.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

Only 100 of 108 total aligned reads displayed.

TCCGCGTATGCAGGATTACACCCTGGAAGCGGATGAAGGTCGCGACATGATGCTGCTGGATGCGCTTATCCAGCTAAAAGAGAAAGATCCCAGCCTGTCGTTCCGCCGCTCCTGCCGTGAAGGTGTGTGCGGTTCCGACGGTCTGAACATGAACGGCAAGAATGGTCTGGCCTGTATTACCCCGATTTCGGCACTCAAC  >  NC_000913/757739‑757937

tCCGCGTATGCAGGATTACACCCTGGAAGCGGATGAAGGTCGCGACATGATGCTGCTGGATGCGCTTATCCAGCTAAAAGAGAAAGATCCCAGCCTGTCg                                                                                                     <  1:1489006/100‑1 (MQ=255)

  cgcgTATGCAGGATTACACCCTGGAAGCGGATGAAGGTCGCGACATGATGCTGCTGGATGCGCTTATCCAGCTAAAAGAGAAAGATCCCAGCCTGTCGtt                                                                                                   <  1:2518378/100‑1 (MQ=255)

         gCAGGATTACACCCTGGAAGCGGATGAAGGTCGCGACATGATGCTGCTGGATGCGCTTATCCAGCTAAAAGAGAAAGATCCCAGCCTGTCGTTCcgccgc                                                                                            <  2:1117494/100‑1 (MQ=255)

         gCAGGATTACACCCTGGAAGCGGATGAAGGTCGCGACATGATGCTGCTGGATGCGCTTATCCAGCTAAAAGAGAAAGATCCCAGCCTGTCGTTCcgccgc                                                                                            <  2:3285821/100‑1 (MQ=255)

          cAGGATTACACCCTGGAAGCGGATGAAGGTCGCGACATGATGCTGCTGGATGCGCTTATCCAGCTAAAAGAGAAAGATCCCAGCCTGTCGTTCcgccgc                                                                                            >  2:1339270/1‑99 (MQ=255)

          cAGGATTACACCCTGGAAGCGGATGAAGGTCGCGACATGATGCTGCTGGATGCGCTTATCCAGCTAAAAGAGAAAGATCCCAGCCTGTCGTTCCGCCGCt                                                                                           <  2:1095139/100‑1 (MQ=255)

          cAGGATTACACCCTGGAAGCGGATGAAGGTCGCGACATGATGCTGCTGGATGCGCTTATCCAGCTAAAAGAGAAAGATCCCAGCCTGTCGTTCCGCCGCt                                                                                           >  2:3334619/1‑100 (MQ=255)

          cAGGATTACACCCTGGAAGCGGATGAAGGTCGCGACATGATGCTGCTGGATGCGCTTATCCAGCTAAAAGAGAAAGATCCCAGCCTGTCGTTCCGCCGCt                                                                                           <  2:3302291/100‑1 (MQ=255)

          cAGGATTACACCCTGGAAGCGGATGAAGGTCGCGACATGATGCTGCTGGATGCGCTTATCCAGCTAAAAGAGAAAGATCCCAGCCTGTC‑TTCCGCCGCTc                                                                                          >  1:277037/1‑100 (MQ=255)

               ttACACCCTGGAAGCGGATGAAGGTCGCGACATGATGCTGCTGGATGCGCTTATCCAGCTAAAAGAGAAAGATCCCACCCTGTCGTTCCGCCGCTCCTGc                                                                                      <  1:1984789/100‑1 (MQ=255)

                tACACCCTGGAAGCGGATGAAGGTCGCGACATGATGCTGCTGGATGCGCTTATCCAGCTAAAAGAGAAAGATCCCAGCCTGTCGTTCCGCCGCTCCTGcc                                                                                     <  2:3028140/100‑1 (MQ=255)

                 acacCCTGGAAGCGGATGAAGGTCGCGACATGATGCTGCTGGATGCGCTTATCCAGCTAAAAGAGAAAGATCCCAGCCTGTCGTTCCGCCGCTCCTGCCg                                                                                    <  2:1943997/100‑1 (MQ=255)

                 acacCCTGGAAGCGGATGAAGGTCGCGAAATGATGCTGCTGGATGAGATTATACAGCTAAAAGAGAAAGATCCCAGCCTGTC‑TTCCGACGCTCCTg                                                                                       >  1:3110209/1‑96 (MQ=255)

                  cacCCTGGAAGCGGATGAAGGTCGCGACATGATGCTGCTGGATGCGCTTATCCAGCTAAAAGAGAAAGATCCCAGCCTGTCGTTCCGCCGCTCCTGCCg                                                                                    <  1:72804/99‑1 (MQ=255)

                  cacCCTGGAAGCGGATGAAGGTCGCGACATGATGCTGCTGGATGCGCTTATCCAGCTAAAAGAGAAAGATCCCAGCCTGTCGTTCCGCCGCTCCTGCCGt                                                                                   <  2:488678/100‑1 (MQ=255)

                  cacCCTGGAAGCGGATGAAGGTCGCGACATGATGCTGCTGGATGCGCTTATCCAGCTAAAAGAGAAAGATCCCAGCCTGTCGTTCCGCCGCTCCTGCCGt                                                                                   >  2:441399/1‑100 (MQ=255)

                  cacCCTGGAAGCGGATGAAGGTCGCGACATGATGCTGCTGGATGCGCTTATCCAGCTAAAAGAGAAAGATCCCAGCCTGTCGTTCCGCCGCTCCTGCCGt                                                                                   >  1:236326/1‑100 (MQ=255)

                    ccttGGAAGCGGATGATGGTCGCGACATGATGCTGCTGGATGCGCTTATCCAGCTAAAAGAGAAAGATCCTAGCCTGTCGTTCCGCCGCTTCTGCCGTg                                                                                  <  1:1734804/96‑1 (MQ=255)

                    cccTGGAAGCGGATGAAGGTCGCGACATGATGCTGCTGGATGCGCTTATCCAGCTAAAAGAGAAAGATCCCAGCCTGTCGTTCCGCCGCTCCTGCCGTGa                                                                                 >  2:954670/1‑100 (MQ=255)

                    cccTGGAAGCGGATGAAGGTCGCGACATGATGCTGCTGGATGCGCTTATCCAGCTAAAAGAGAAAGATCCCAGCCTGTCGTTCCGCCGCTCCTGCCGTGa                                                                                 >  2:376897/1‑100 (MQ=255)

                    cccTGGAAGCGGATGAAGGTCGCGACATGATGCTGCTGGATGCGCTTATCCAGCTAAAAGAGAAAGATCCCAGCCTGTCGTTCCGCCGCTCCTGCCGTGa                                                                                 >  2:196112/1‑100 (MQ=255)

                    cccTGGAAGCGGATGAAGGTCGCGACATGATGCTGCTGGATGCGCTTATCCAGCTAAAAGAGAAAGATCCCAGCCTGTC‑TTCCGCCGCTCCTGCCGTGaa                                                                                <  2:2801291/100‑1 (MQ=255)

                    cccTGGAAGCGGATGAAGGTCGCGACATGATGCTGCTGGATGCGCTTATCCAGCTAAAAGAGAAAGATCCCAGCCTGTC‑TTCCGCCGCTCCTGCCGTGaa                                                                                >  2:2081317/1‑100 (MQ=255)

                    cccTGGAAGCGGATGAAGGTCGCGACATGATGCTGCTGGATGCGCTTATCCAGCTAAAAGAGAAAGATCCCAGCCTGTC‑TTCCGCCGCTCCTGCCGTGaa                                                                                >  2:2457536/1‑100 (MQ=255)

                     ccTGGAAGCGGATGAAGGTCGCGACATGATGCTGCTGGATGCGCTTATCCAGCTAAAAGAGAAAGATCCCAGCCTGTC‑TTCCGCCGCTCCTGCCGTGAAg                                                                               >  1:2044981/1‑100 (MQ=255)

                        ggAAGCGGATGAAGGTCGCGACATGATGCTGCTGGATGCGCTTATCCAGCTAAAAGAGAAAGATCCCAGCCTGTCGTTCCGCCGCTCCTGCCGTGAAgg                                                                              <  1:2862923/99‑1 (MQ=255)

                        ggAAGCGGATGAAGGTCGCGACATGATGCTGCTGGATGCGCTTATCCAGCTAAAAGAGAAAGATCCCAGCCTGTC‑TTCCGCCGCTCCTGCCGTGAAGgtg                                                                            <  2:2492167/100‑1 (MQ=255)

                           aGCGGATGAAGGTCGCGACATGATGCTGCTGGATGCGCTTATCCAGCTAAAAGAGAAAGATCCCAGCCTGTCGTTCCGCCGCTCCTGCCGTGAAGgtgtg                                                                          <  2:1416575/100‑1 (MQ=255)

                           aGCGGATGAAGGTCGCGACATGATGCTGCTGGATGCGCTTATCCAGCTAAAAGAGAAAGATCCCAGCCTGTCGTTCCGCCGCTCCTGCCGTGAAGgtgtg                                                                          <  1:1351752/100‑1 (MQ=255)

                             cGGATGAAGGTCGCGACATGATGCTGCTGGATGCGCTTATCCAGCTAAAAGAGAAAGATCCCAGCCTGTCGTTCCGCCGCTCCTGCCGTGAAGGtgtgtg                                                                        <  1:1056017/100‑1 (MQ=255)

                             cGGATGAAGGTCGCGACATGATGCTGCTGGATGCGCTTATCCAGCTAAAAGAGAAAGATCCCAGCCTGTCGTTCCGCCGCTCCTGCCGTGAAGGtgtgtg                                                                        <  1:329401/100‑1 (MQ=255)

                              ggATGAAGGTCGCGACATGATGCTGCTGGATGCGCTTATCCAGCTAAAAGAGAAAGATCCCAGCCTGTCGTTCCGCCGCTCCTGCCGTGAAGGTGTGTGc                                                                       <  1:2180586/100‑1 (MQ=255)

                              ggATGAAGGTCGCGACATGATGCTGCTGGATGCGCTTATCCAGCTAAAAGAGAAAGATCCCAGCCTGTC‑TTCCGCCGCTCCTGCCGTGAAGGTGTGTGCg                                                                      >  2:3231455/1‑100 (MQ=255)

                                    aGGTCGCGACATGATGCTGCTGGATGCGCTTATCCAGCTAAAAGAGAAAGATCCCAGCCTGTCGTTCCGCCGCTCCTGCCGTGAAGGTGTGTGCGGTTcc                                                                 <  2:236328/100‑1 (MQ=255)

                                     ggTCGCGACATGATGCTGCTGGATGCGCTTATCCAGCTAAAAGAGAAAGATCCCAGCCTGTCGTTCCGCCGCTCCTGCCGTGAAGGTGTGTGCGGTTCCg                                                                >  2:2637520/1‑100 (MQ=255)

                                     ggTCGCGACATGATGCTGCTGGATGCGCTTATCCAGCTAAAAGAGAAAGATCCCAGCCTGTC‑TTCCGCCGCTCCTGCCGTGAAGGTGTGTGCGGTTCCGa                                                               <  2:2422899/100‑1 (MQ=255)

                                      gTCGCGACATGATGCTGCTGGATGCGCTTATCCAGCTAAAAGAGAAAGATCCCAGCCTGTCGTTCCGCCGCTCCTGCCGTGAAGGTGTGTGCGGTTCCGa                                                               >  1:36434/1‑100 (MQ=255)

                                      gTCGCGACATGATACTGCTGGATGCGCTTATCCAGCTAAAAGAGAAAGATCCCAGCCTGTCGTTCCGCCGCTCCTGCCGTGAAGGTGTGTGCGATTCCGa                                                               >  2:1135585/1‑100 (MQ=255)

                                       tCGCGACATGATGCTGCTGGATGCGCTTATCCAGCTAAAAGAGAAAGATCCCAGCCTGTCGTTCCGCCGCTCCTGCCGTGAAGGTGTGTGCGGTTCCGAc                                                              <  2:2315916/100‑1 (MQ=255)

                                        cgcgACATGATGCTGCTGGATGCGCTTATCCAGCTAAAAGAGAAAGATCCCAGCCTGTC‑TTCCGCCGCTCCTGCCGTGAAGGTGTGTGCGGTTCCGACg                                                             <  2:1094664/99‑1 (MQ=255)

                                          cgACATGATGCTGCTGGATGCGCTTATCCAGCTAAAAGAGAAAGATCCCAGCCTGTCGTTCCGCCGCTCCTGCCGTGAAGGTGTGTGCGGTTCCGACGGt                                                           >  2:1423127/1‑100 (MQ=255)

                                          cgACATGATGCTGCTGGATGCGCTTATCCAGCTAAAAGAGAAAGATCCCAGCCTGTC‑TTCCGCCGCTCCTGCCGTGAAGGTGTGTGCGGTTCCGACGGTc                                                          >  1:1930312/1‑100 (MQ=255)

                                           gACATGATGCTGCTGGATGCGCTTATCCAGCTAAAAGAGAAAGATCCCAGCCTGTCGTTCCGCCGCTCCTGCCGTGAAGGTGTGTGCGGTTCCGACGGTc                                                          <  2:1315635/100‑1 (MQ=255)

                                               tgatgCTGCTGGATGCGCTTATCCAGCTAAAAGAGAAAGATCCCAGCCTGTCGTTCCGCCGCTCCTGCCGTGAAGGTGTGTGCGGTTCCGACGGTCTGaa                                                      <  1:2806651/100‑1 (MQ=255)

                                               tgatgCTGCTGGATGCGCTTATCCAGCTAAAAGAGAAAGATCCCAGCCTGTCGTTCCGCCGCTCCTGCCGTGAAGGTGTGTGCGGTTCCGACGGTCTGaa                                                      >  1:3113766/1‑100 (MQ=255)

                                                gatgCTGCTGGATGCGCTTATCCAGCTAAAAGAGAAAGATCCCAGCCTGTCGTTCCGCCGCTCCTGCCGTGAAGGTGTGTGCGGTTCCGACGGTCTGAAc                                                     <  2:2804512/100‑1 (MQ=255)

                                                  tgcttcTGGATGCGCTTATCCAGCTAAAAGAGAAAGATCCCAGCCTGTC‑TTCCGCCGCTCCTGCCGTGAAGGTGTGTGCGGTTCCGACGGTCTGAACATg                                                  <  1:1263665/100‑1 (MQ=255)

                                                  tgctgcTGGATGCGCTTATCCAGCTAAAAGAGAAAGATCCCAGCCTGTCGTTCCGCCGCTCCTGCCGTGAAGGTGTGTGCGGTTCCGACGGTCTGAACAt                                                   >  2:654174/1‑100 (MQ=255)

                                                  tactgctgGATGCGCTTATACCGCTACAAGAGACCGATCCCCGGCTGTCGTTCCGCCGCTCCTGCCGTGAAGGTGTGTGCGGTTCCGACGGTCTGAACa                                                    <  1:1135581/97‑1 (MQ=255)

                                                   gctgctGGATGCGCTTATCCAGCTAAAAGAGAAAGATCCCAGCCTGTCGTTCCGCCGCTCCTGCCGTGAAGGTGTGTGCGGTTCCGACGGTCTGAACATg                                                  <  2:3127105/100‑1 (MQ=255)

                                                   gctgctGGATGCGCTTATCCAGCTAAAAGAGAAAGATCCCAGCCTGTCGTTCCGCCGCTCCTGCCGTGAAGGTGTGTGCGGTTCCGACGGTCTGAACATg                                                  >  1:2015687/1‑100 (MQ=255)

                                                    ctgctgGATGCGCTTATCCAGCTAAAAGAGAAAGATCCCAGCCTGTCGTTCCGCCGCTCCTGCCGTGAAGGTGTGTGCGGTTCCGACGGTCTGAACATGa                                                 >  1:2198551/1‑100 (MQ=255)

                                                    ctgctgGATGCGCTTATCCAGCTAAAAGAGAAAGATCCCAGCCTGTCGTTCCGCCGCTCCTGCCGTGAAGGTGTGTGCGGTTCCGACGGCCTGAACATGa                                                 >  1:2041347/1‑100 (MQ=255)

                                                      gctgGATGCGCTTATCCAGCTAAAAGAGAAAGATCCCAGCCTGTCGTTCCGCCGCTCCTGCCGTGAAGGTGTGTGCGGTTCCGACGGTCTGAACATGAAc                                               <  1:244746/100‑1 (MQ=255)

                                                      gctgGATGCGCTTATCCAGCTAAAAGAGAAAGATCCCAGCCTGTC‑TTCCGCCGCTCCTGCCGTGAAGGTGTGTGCGGTTCCGACGGTCTGAACATGAACg                                              <  2:112492/100‑1 (MQ=255)

                                                       ctgGATGCGCTTATCCAGCTAAAAGAGAAAGATCCCAGCCTGTCGTTCCGCCGCTCCTGCCGTGAAGGTGTGTGCGGTTCCGACGGTCTGAACATGAACg                                              >  2:566931/1‑100 (MQ=255)

                                                         ggATGCGCTTATCCAGCTAAAAGAGAAAGATCCCAGCCTGTCGTTCCGCCGCTCCTGCCGTGAAGGTGTGTGCGGTTCCGACGGTCTGAACATGAACgtc                                            <  2:2315733/100‑3 (MQ=255)

                                                         ggATGCGCTTATCCAGCTAAAAGAGAAAGATCCCAGCCTGTC‑TTCCGCCGCTCCTGCCGTGAAGGTGTGTGCGGTTCCGACGGTCTGAACATGAACGGCa                                           >  2:767981/1‑100 (MQ=255)

                                                         ggATGCGCTTATCCAGCTAAAAGAGAAAGAACCCAGCCTGTC‑TTCCGCCGCTCCAGCCGTGAAGGTGTGTGCGGTTCCGACGGTCTGAACATGAACGCCa                                           >  1:832483/1‑100 (MQ=255)

                                                          atctcgcTTATCCAGCTAAAAGAGAAAGATCCCAGCCTGTCGTTCCGCCGCTCCTGCCGTGAAGGTGTGTGCGGTTCCGACGGTCTGAACATGAACGGCa                                           <  2:3072379/96‑1 (MQ=255)

                                                           aTGCGCTTATCCAGCTAAAAGAGAAAGATCCCAGCCTGTCGTTCCGCCGCTCCTGCCGTGAAGGTGTGTGCGGTTCCGACGGTCTGAACATGAACGGCaa                                          >  2:1316129/1‑100 (MQ=255)

                                                             gcgcTTATCCAGCTAAAAGAGAAAGATCCCAGCCTGTCGTTCCGCCGCTCCTGCCGTGAAGGTGTGTGCGGTTCCGACGGTCTGAACATGAACGGCAAGa                                        <  1:82603/100‑1 (MQ=255)

                                                             gcgcTTATCCAGCTAAAAGAGAAAGATCCCAGCCTGTCGTTCCGCCGCTCCTGCCGTGAAGGTGTGTGCGGTTCCGACGGTCTGAACATGAACGGCAAGa                                        >  2:2535856/1‑100 (MQ=255)

                                                              cgcTTATCCAGCTAAAAGAGAAAGATCCCAGCCTGTCGTTCCGCCGCTCCTGCCGTGAAGGTGTGTGCGGTTCCGACGGTCTGAACATGAACGGCAAGat                                       >  1:3072362/1‑99 (MQ=255)

                                                              cgcTTATCCAGCTAAAAGAGAAAGATCCCAGCCTGTCGTTCCGCCGCTCCTGCCGTGAAGGTGTGTGCGGTTCCGACGGTCTGAACATGAACGGCAAGaa                                       >  2:2493907/1‑100 (MQ=255)

                                                              cgcCTATCCAGCTAAAAGAGAAAGATCCCAGCCTGTC‑TTCCGCCGCTCCTGCCGTGAAGGTGTGTGCGGTTCCGACGGTCTGAACATGAACGGCAAGAAt                                      >  2:1338761/1‑100 (MQ=255)

                                                                cTTATCCAGCTAAAAGAGAAAGATCCCAGCCTGTCGTTCCGCCGCTCCTGCCGTGAAGGTGTGTGCGGTTCCGACGGTCTGAACATGAACGGCAAGAATg                                     <  2:910145/100‑1 (MQ=255)

                                                                    tCCAGCTAAAAGAGAAAGATCCCAGCCTGTCGTTCCGCCGCTCCTGCCGTGAAGGTGTGTGCGGTTCCGACGGTCTGAACATGAACGGCAAGAATGGTCt                                 >  1:1604249/1‑100 (MQ=255)

                                                                    tCCAGCTAAAAGAGAAAGATCCCAGCCTGTC‑TTCCGCCGCTCCTGCCGTGAAGGTGTGTGCGGTTCCGACGGTCTGAACATGAACGGCAAGAATGGTCTg                                <  2:2979996/100‑1 (MQ=255)

                                                                     ccAGCTAAAAGAGAAAGATCCCAGCCTGTCGTTCCGCCGCTCCTGCCGTGAAGGTGTGTGCGGTTCCGACGGTCTGAACATGAACGGCAAGAATGGTCTg                                <  2:2154534/100‑1 (MQ=255)

                                                                     ccAGCTAAAAGAGAAAGATCCCAGCCTGTCGTTCCGCCGCTCCTGCCGTGAAGGTGTGTGCGGTTCCGACGGTCTGAACATGAACGGCAAGAATGGTCTg                                >  1:2374799/1‑100 (MQ=255)

                                                                      cAGCTAAAAGAGAAAGATCCCAGCCTGTCGTTCCGCCGCTCCTGCCGTGAAGGTGTGTGCGGTTCCGACGGTCTGAACATGAACGGCAAGAATGGTCTgg                               <  1:1459020/100‑1 (MQ=255)

                                                                      cAGCTAAAAGAGAAAGATCCCAGCCTGTCGTTCCGCCGCTCCTGCCGTGAAGGTGTGTGCGGTTCCGACGGTCTGAACATGAACGGCAAGAATGGTCTg                                >  2:1381700/1‑99 (MQ=255)

                                                                       aGCTAAAAGAGAAAGATCCCAGCCTGTC‑TTCCGCCGCTCCTGCCGTGAAGGTGTGTGCGGTTCCGACGGTCTGAACATGAACGGCAAGAATGGTCTGGcc                             >  2:1495304/1‑100 (MQ=255)

                                                                       aGCTAAAAGAGAAAGATCCCAGCCTGTC‑TTCCGCCGCTCCTGCCGTGAAGGTGTGTGCGGTTCCGACGGTCTGAACATGAACGGCAAGAATGGTCTGGcc                             <  2:1860548/100‑1 (MQ=255)

                                                                          tAAAAGAGAAAGATCCCAGCCTGTCGTTCCGCCGCTCCTGCCGTGAAGGTGTGTGCGGTTCCGACGGTCTGAACATGAACGGCAAGAATGGTCTGGCCTg                           >  2:792225/1‑100 (MQ=255)

                                                                           aaaaGAGAAAGATCCCAGCCTGTCGTTCCGCCGCTCCTGCCGTGAAGGTGTGTGCGGTTCCGACGGTCTGAACATGAACGGCAAGAATGGTCTGGCCTGt                          >  1:3202378/1‑100 (MQ=255)

                                                                            aaaGAGAAAGATCCCAGCCTGTCGTTCCGCCGCTCCTGCCGTGAAGGTGTGTGCGGTTCCGACGGTCTGAACATGAACGGCAAGAATGGTCTGGCCTGTa                         <  2:1605794/100‑1 (MQ=255)

                                                                             atgagaAAGATCCCAGCCTGTC‑TTCCGCCGCTCCTGCCGTGAAGGTGTGTGCGGTTCCGACGGTCTGAACATGAACGGCCAGAATGGTCTGGCCTGTAtt                       <  1:2081306/98‑1 (MQ=255)

                                                                             aaGAGAAAGTTCCTAGCCTTTCGTTTCTTCTCTCCTGCCGTGAAGGTGTGTGCGGTGCCGACGGTCTGAACATTAACGGCAATAATGGTCTGGCCTGTAt                        <  1:173433/100‑1 (MQ=255)

                                                                             aaGAGAAAGATCCCAGCCTGTCGTTCCGCCGCTCCTGCCGTGAAGGTGTGTGCGGTTCCGACGGTCTGAACATGAACGGCAAGAATGGTCTGGCCTGTAt                        >  1:2904930/1‑100 (MQ=255)

                                                                              agagAAAGATCCCAGCCTGTCGTTCCGCCGCTCCTGCCGTGAAGGTGTGTGCGGTTCCGACGGTCTGAACATGAACGGCAAGAATGGTCTGGCCTGTAt                        >  1:692472/1‑99 (MQ=255)

                                                                               gagaAAGATCCCAGCCTGTC‑TTCCGCCGCTCCTGCCGTGAAGGTGTGTGCGGTTCCGACGGTCTGAACATGAACGGCAAGAATGGTCTGGCCTGTATTAc                     >  2:1777737/1‑100 (MQ=255)

                                                                                agaAAGATCCCAGCCTGTCGTTCCGCCGCTCCTGCCGTGAAGGTGTGTGCGGTTCCGACGGTCTGAACATGAACGGCAAGAATGGTCTGGCCTGTATTAc                     <  2:898105/100‑1 (MQ=255)

                                                                                 gaAAGATCCCAGCCTGTCGTTCCGCCGCTCCTGGCGTGAAGGTGTGTGCGGTTCCGACGGTCTGAACATGAACGGCAAGAATGGTCTGGACTGTATTAcc                    >  1:2242874/1‑100 (MQ=255)

                                                                                 gaAAGATCCCAGCCTGTCGTTCCGCCGCTCCTGCCGTGAAGGTGTGTGCGGTTCCGACGGTCTGAACATGAACGGCAAGAATGGTCTGGCCTGTATTAcc                    >  1:1080707/1‑100 (MQ=255)

                                                                                     gATCCCAGCCTGTCGTTCCGCCGCTCCTGCCGTGAAGGTGTGTGCGGTTCCGACGGTCTGAACATGAACGGCAAGAATGGTCTGGCCTGTATTACCCCGa                <  1:672325/100‑1 (MQ=255)

                                                                                     gATCCCAGCCTGTC‑TTCCGCCGCTCCTGCCGTGAAGGTGTGTGCGGTTCCGACGGTCTGAACATGAACGGCAAGAATGGTCTGGCCTGTATTACCCCGAt               >  2:1955918/1‑100 (MQ=255)

                                                                                       tCCCAGCCTGTCGTTCCGCCGCTCCTGCCGTGAAGGTGTGTGCGGTTCCGACGGTCTGAACATGAACGGCAAGAATGGTCTGGCCTGTATTACCCCGAtt              <  1:234922/100‑1 (MQ=255)

                                                                                       tCCCAGCCTGTCGTTCCGCCGCTCCTGCCGTGAAGGTGTGTGCGGTTCCGACGGTCTGAACATGAACGGCAAGAATGGTCTGGCCTGTATTACCCCGAtt              >  2:5695/1‑100 (MQ=255)

                                                                                          cAGCCTGTC‑TTCCGCCGCTCCTGCCGTGAAGGTGTGTGCGGTTCCGACGGTCTGAACATGAACGGCAAGAATGGTCTGGCCTGTATTACCCCGATTTCgg          <  1:737791/100‑1 (MQ=255)

                                                                                           aGCCTGTCGTTCCGCCGCTCCTGCCGTGAAGGTGTGTGCGGTTCCGACGGTCTGAACATGAACGGCAAGAATGGTCTGGCCTGTATTACCCCGATTTCgg          <  2:3140602/100‑1 (MQ=255)

                                                                                           aGCCTGTCGTTCCGCCGCTCCTGCCGTGAAGGTGTGTGCGGTTCCGACGGTCTGAACATGAACGGCAAGAATGGTCTGGCCTGTATTACCCCGATTTCgg          <  2:514976/100‑1 (MQ=255)

                                                                                           aGCCTGTCGTTCCGCCGCTCCTGCCGTGAAGGTGTGTGCGGTTCCGACGGCCTGAACATGAACGGCAAGAATGGTCTGGCCTGTATTACCCCGATTTCgg          <  2:2041358/100‑1 (MQ=255)

                                                                                           aGCCTGTC‑TTCCGCCGCTCCTGCCGTGAAGGTGTGTGCGGTTCCGACGGTCTGAACATGAACGGCAAGAATGGTCTGGCCTGTATTACCCCGATTTCGGc         <  2:1582360/100‑1 (MQ=255)

                                                                                            gCCTGTCGTTCCGCCGCTCCTGCCGTGAAGGTGTGTGCGGTTCCGACGGTCTGAACATGAACGGCAAGAATGGTCTGGCCTGTATTACCCCGATTTCg           <  1:2031707/98‑1 (MQ=255)

                                                                                            gCCTGTCGTTCCGCCGCTCCTGCCGTGAAGGTGTGTGCGGTTCCGACGGTCTGAACATGAACGGCAAGAATGGTCTGGCCTGTATTACCCCGATTTCGGc         <  2:2764997/100‑1 (MQ=255)

                                                                                            gCCTGTCGTTCCGCCGCTCCTGCCGGGAAGGTGTGTGCGGTTCCGACGGTCTGAACATGATCGGCAAGAATGGTCTGGCCTGTATTACCCCGATTTCgn          <  1:1321541/99‑2 (MQ=255)

                                                                                             ccTGTCGTTCCGCCGCTCCTGCCGTGAAGGTGTGTGCGGTTCCGACGGTCTGAACATGAACGGCAAGAATGGTCTGGCCTGTATTACCCCGATTTCGGCa        <  1:792223/100‑1 (MQ=255)

                                                                                                   gTTCCGCCGCTCCTGCCGTGAAGGTGTGTGCGGTTCCGACGGTCTGAACATGAACGGCAAGAATGGTCTGGCCTGTATTACCCCGATTTCGGCACTCAAc  <  2:2273753/100‑1 (MQ=255)

TCCGCGTATGCAGGATTACACCCTGGAAGCGGATGAAGGTCGCGACATGATGCTGCTGGATGCGCTTATCCAGCTAAAAGAGAAAGATCCCAGCCTGTCGTTCCGCCGCTCCTGCCGTGAAGGTGTGTGCGGTTCCGACGGTCTGAACATGAACGGCAAGAATGGTCTGGCCTGTATTACCCCGATTTCGGCACTCAAC  >  NC_000913/757739‑757937

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A