Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A8 F50 I0 R1 26 138.6 6843716 94.5% 6467311 99.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,810,760 T→C 100% intergenic (+81/‑10) ygaH → / → mprA putative L‑valine exporter, norvaline resistance protein/transcriptional repressor of microcin B17 synthesis and multidrug efflux

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,810,7600TC99.3% 466.7 / ‑4.7 137intergenic (+81/‑10)ygaH/mprAputative L‑valine exporter, norvaline resistance protein/transcriptional repressor of microcin B17 synthesis and multidrug efflux
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (72/64);  total (73/64)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.58e-01

Only 100 of 137 total aligned reads displayed.

GATGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGA  >  NC_000913/2810664‑2810857
                                                                                                |                                                                                                 
gATGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCAt                                                                                                >  1:1156923/1‑100 (MQ=255)
 aTGGCGATTATATAACCCACAAGAATCATTTTTCTAATACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATa                                                                                               >  1:344527/1‑100 (MQ=255)
  tGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATAc                                                                                              >  2:1794337/1‑100 (MQ=255)
  tGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATAc                                                                                              <  1:965512/100‑1 (MQ=255)
  tGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATAc                                                                                              <  1:889699/100‑1 (MQ=255)
   ggCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATAcc                                                                                             <  1:1753996/100‑1 (MQ=255)
    gCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATAccc                                                                                            <  2:2370987/100‑1 (MQ=255)
     cGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCa                                                                                           >  2:2351823/1‑100 (MQ=255)
       aTTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCaaa                                                                                         >  2:777792/1‑100 (MQ=255)
         tatataACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATg                                                                                       <  2:649724/100‑1 (MQ=255)
         tatataACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATg                                                                                       <  2:210158/100‑1 (MQ=255)
         tatataACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATg                                                                                       <  1:1004959/100‑1 (MQ=255)
            ataACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGAt                                                                                    <  1:738574/100‑1 (MQ=255)
               aCCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTATCGTTACTATATCGTCTGAAATTAATGAGGCCATACCCAAATGGATAGt                                                                                 >  1:2367078/1‑100 (MQ=255)
                cccACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGtt                                                                                >  1:442714/1‑100 (MQ=255)
                cccACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGtt                                                                                >  2:3156986/1‑100 (MQ=255)
                cccACAAGAAGCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGtt                                                                                >  1:2405982/1‑100 (MQ=255)
                  cacaAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCg                                                                              >  2:1104976/1‑100 (MQ=255)
                  cacaAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCg                                                                              >  2:1199591/1‑100 (MQ=255)
                   acaAGAATCATTTTGCTAAAACAATACATTGACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGt                                                                             <  1:2613243/100‑1 (MQ=255)
                     aaGTATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCATTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGttt                                                                           <  1:331120/100‑1 (MQ=255)
                      aGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTa                                                                          >  2:92587/1‑100 (MQ=255)
                       gAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTAc                                                                         >  2:1661851/1‑100 (MQ=255)
                          tCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGcc                                                                      <  1:578943/100‑1 (MQ=255)
                             tttttCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCAt                                                                   >  1:2950481/1‑100 (MQ=255)
                              ttttCTAAAACAATATATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCa                                                                    <  1:2037926/98‑1 (MQ=255)
                               tttCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTg                                                                 <  2:917560/100‑1 (MQ=255)
                               tttCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTg                                                                 >  1:3195739/1‑100 (MQ=255)
                               tttCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTg                                                                 <  1:2163732/100‑1 (MQ=255)
                                   tAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACa                                                             >  2:3057331/1‑100 (MQ=255)
                                   tAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACa                                                             <  1:1101280/100‑1 (MQ=255)
                                    aaaaCAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACaa                                                            >  2:1270045/1‑100 (MQ=255)
                                       aCAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAAt                                                          >  1:218198/1‑99 (MQ=255)
                                       aCAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATg                                                         <  1:1033580/100‑1 (MQ=255)
                                         aaTACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCt                                                       <  2:2001692/100‑1 (MQ=255)
                                          aTACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTa                                                      >  2:879561/1‑100 (MQ=255)
                                            aCATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTaaa                                                    <  1:423605/100‑1 (MQ=255)
                                             cATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTaaaa                                                   <  2:820350/100‑1 (MQ=255)
                                             cATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTaaaa                                                   >  2:1208770/1‑100 (MQ=255)
                                             cATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTaaaa                                                   >  2:3033125/1‑100 (MQ=255)
                                             cATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTaaaa                                                   >  2:1110222/1‑100 (MQ=255)
                                             cATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACACAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTaaaa                                                   >  1:3115443/1‑100 (MQ=255)
                                              aTTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAAt                                                  >  2:233502/1‑100 (MQ=255)
                                              aTTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAAt                                                  >  2:51944/1‑100 (MQ=255)
                                              aTTTACTTTATTTGTAACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCAAAACAACATGTATAGTCTGTTTACTCCGATTGAATAAATGCTaaa                                                    >  1:3178100/1‑98 (MQ=255)
                                                 tACTTTATTTGTCACTGTCGATACGATATCGGCTGCAATTTATGAGGCCATACCAAAATTGATATATAGGTTGCGCCCATTGACCACATTGTAAAATATc                                               >  1:1434752/1‑100 (MQ=255)
                                                  aCTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTcg                                              <  2:442716/100‑1 (MQ=255)
                                                  aCTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTcg                                              <  1:3255530/100‑1 (MQ=255)
                                                  aCTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTcg                                              >  2:1726503/1‑100 (MQ=255)
                                                   cTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTcgc                                             <  2:2950499/100‑1 (MQ=255)
                                                   cTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTcgc                                             >  1:2986123/1‑100 (MQ=255)
                                                   cTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTcg                                              <  1:1577759/99‑1 (MQ=255)
                                                   cTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATAGATAGTTCGTGTACGCCCATTGAACAAACGCTAAAATTTccc                                             <  2:2308950/100‑3 (MQ=255)
                                                     ttATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCgcgc                                           >  2:1962757/1‑100 (MQ=255)
                                                     ttATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCgcgc                                           >  1:1270318/1‑100 (MQ=255)
                                                       aTTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCa                                         >  2:2652706/1‑100 (MQ=255)
                                                        tttGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAg                                        >  1:2544644/1‑100 (MQ=255)
                                                          tGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAgcc                                      >  2:2254841/1‑100 (MQ=255)
                                                          tGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAgcc                                      >  1:2912492/1‑100 (MQ=255)
                                                          tGTCACTGTCGTTACTATATCGGCTGAAATTAATACGGCCATACCCAAATGGATCGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAgcc                                      >  1:324734/1‑100 (MQ=255)
                                                               cTGTCGTTACTATATCGACTGAAATTAATGAAGCCATACCCAAATGGATAGTGCGTTTACGACCATTGAACAAATGCTAAAAATTCGCGCCAgccgc                                    >  1:1207206/1‑97 (MQ=255)
                                                                tGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACg                                >  1:2815917/1‑100 (MQ=255)
                                                                 gTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGa                               >  1:2777913/1‑100 (MQ=255)
                                                                   ngTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAg                             >  2:563306/2‑100 (MQ=255)
                                                                    gTTACTATATCGGCTGAAATTAATGATGCCATACCCTTATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGa                            <  2:1136992/100‑1 (MQ=255)
                                                                    gTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGa                            >  2:810291/1‑100 (MQ=255)
                                                                     ttACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGAt                           <  2:2519380/100‑1 (MQ=255)
                                                                     ttACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGAt                           >  1:764872/1‑100 (MQ=255)
                                                                     ttACTATATCGGCTGAAATTAATGACGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCTGCCGCCACGAAGa                            <  1:2111630/99‑1 (MQ=255)
                                                                      tACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGAtt                          <  1:2912638/100‑1 (MQ=255)
                                                                       aCTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGAttt                         <  2:875237/100‑1 (MQ=255)
                                                                       aCTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGAttt                         <  1:2429378/100‑1 (MQ=255)
                                                                        cTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGAtttt                        <  1:568617/100‑1 (MQ=255)
                                                                         tataTCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGAtttt                        <  2:1484434/99‑1 (MQ=255)
                                                                         tataTCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTc                       >  2:411025/1‑100 (MQ=255)
                                                                          atatCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTcc                      >  2:813969/1‑100 (MQ=255)
                                                                          atatCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTcc                      <  2:1928688/100‑1 (MQ=255)
                                                                           tatCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCt                     >  1:1188437/1‑100 (MQ=255)
                                                                            atCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCtt                    >  1:3364330/1‑100 (MQ=255)
                                                                             tCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTa                   <  1:369203/100‑1 (MQ=255)
                                                                              cGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTAt                  >  2:435478/1‑100 (MQ=255)
                                                                               ggCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATc                 >  1:1224949/1‑100 (MQ=255)
                                                                               ggCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATc                 <  2:3270340/100‑1 (MQ=255)
                                                                                gCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCa                <  1:2671627/100‑1 (MQ=255)
                                                                                gCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCa                <  1:2464484/100‑1 (MQ=255)
                                                                                 cTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAg               >  2:754380/1‑100 (MQ=255)
                                                                                 cTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAg               <  1:297972/100‑1 (MQ=255)
                                                                                 cTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAg               <  1:1019703/100‑1 (MQ=255)
                                                                                  tGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAgg              <  2:2910338/100‑1 (MQ=255)
                                                                                  tGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAgg              >  2:2571428/1‑100 (MQ=255)
                                                                                   gAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGga             <  1:92587/100‑1 (MQ=255)
                                                                                     aaTTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGgaga           <  2:992189/100‑1 (MQ=255)
                                                                                      aTTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGAt          <  1:2796789/100‑1 (MQ=255)
                                                                                      aTTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGAt          <  1:2315507/100‑1 (MQ=255)
                                                                                         aaTGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATACt       <  1:1871009/100‑1 (MQ=255)
                                                                                            gAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCt    <  2:2843422/100‑1 (MQ=255)
                                                                                            gAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCt    <  2:1998715/100‑1 (MQ=255)
                                                                                            gAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCt    <  2:1389191/100‑1 (MQ=255)
                                                                                             aGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTg   <  1:744487/100‑1 (MQ=255)
                                                                                              ggTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGa  >  2:3331804/1‑100 (MQ=255)
                                                                                                |                                                                                                 
GATGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGA  >  NC_000913/2810664‑2810857

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A