Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F69 I1 R1 15 111.3 5655542 93.7% 5299242 99.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,810,760 T→C intergenic (+81/‑10) ygaH → / → mprA putative L‑valine exporter, norvaline resistance protein/transcriptional repressor of microcin B17 synthesis and multidrug efflux

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,810,7600TC97.6% 433.2 / ‑2.7 123intergenic (+81/‑10)ygaH/mprAputative L‑valine exporter, norvaline resistance protein/transcriptional repressor of microcin B17 synthesis and multidrug efflux
Reads supporting (aligned to +/- strand):  ref base T (1/2);  new base C (59/61);  total (60/63)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 123 total aligned reads displayed.

GTGATGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTG  >  NC_000913/2810662‑2810856
                                                                                                  |                                                                                                
gTGATGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGTc                                                                                                 <  2:855903/100‑1 (MQ=255)
 tGATGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCa                                                                                                >  2:1758151/1‑100 (MQ=255)
  gATGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCAt                                                                                               >  2:1762404/1‑100 (MQ=255)
    tGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATAc                                                                                             >  2:2636565/1‑100 (MQ=255)
       cGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCa                                                                                          <  1:2313900/100‑1 (MQ=255)
       cGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCa                                                                                          >  1:2072988/1‑100 (MQ=255)
       cGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCa                                                                                          <  2:1018583/100‑1 (MQ=255)
        gATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCaa                                                                                         >  2:531327/1‑100 (MQ=255)
        gATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCaa                                                                                         >  1:13595/1‑100 (MQ=255)
          ttATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGTCATACCCAAAt                                                                                       <  1:1696588/100‑1 (MQ=255)
          ttATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAAt                                                                                       <  2:2636102/100‑1 (MQ=255)
           tatataACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATg                                                                                      <  2:2299743/100‑1 (MQ=255)
            atataACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATgg                                                                                     <  1:2743192/100‑1 (MQ=255)
             tataACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGa                                                                                    >  1:1382170/1‑100 (MQ=255)
              ataACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGAt                                                                                   <  2:1170261/100‑1 (MQ=255)
              ataACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGAt                                                                                   >  2:1274787/1‑100 (MQ=255)
               taACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATa                                                                                  >  1:277396/1‑100 (MQ=255)
               taACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATa                                                                                  >  1:73439/1‑100 (MQ=255)
                aaCCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAg                                                                                 >  2:1706418/1‑100 (MQ=255)
                 aCCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGt                                                                                >  2:1204738/1‑100 (MQ=255)
                  cccACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGtt                                                                               >  1:834600/1‑100 (MQ=255)
                  cccACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGtt                                                                               >  2:676677/1‑100 (MQ=255)
                    cacaAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTc                                                                              <  2:154527/99‑1 (MQ=255)
                    cacaAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTc                                                                              >  2:2234719/1‑99 (MQ=255)
                    cacaAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCg                                                                             >  2:1107856/1‑100 (MQ=255)
                    cacaAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCg                                                                             >  2:2343269/1‑100 (MQ=255)
                    cacaAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCg                                                                             <  2:2753255/100‑1 (MQ=255)
                    cacaAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCg                                                                             <  1:531330/100‑1 (MQ=255)
                    cacaAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACACAAATGGATAGTTCg                                                                             >  2:16099/1‑100 (MQ=255)
                    cacaAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATAGGCTGAAATTAATGAGGTCATACCCAAATGGATAGt                                                                                >  2:121126/1‑97 (MQ=255)
                      caAGAATCATTTTTCTAAAACAATACATTTCCTTTATTTGTCACTGTCGTTAATATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGtt                                                                           <  2:937878/100‑1 (MQ=255)
                      caAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGtt                                                                           >  1:1580079/1‑100 (MQ=255)
                        aGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATCTCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTa                                                                         <  1:1626753/100‑1 (MQ=255)
                        aGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTa                                                                         <  2:1096426/100‑1 (MQ=255)
                         gAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTan                                                                        <  2:2801281/100‑2 (MQ=255)
                         gAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTAc                                                                        >  1:2066063/1‑100 (MQ=255)
                           aTCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGc                                                                      <  2:508902/100‑1 (MQ=255)
                            tCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGcc                                                                     <  1:1234090/100‑1 (MQ=255)
                              aTTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGcc                                                                     >  2:1878491/1‑98 (MQ=255)
                               tttttCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCAt                                                                  <  1:2479298/100‑1 (MQ=255)
                               tttttCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGACCAt                                                                  >  2:2805373/1‑100 (MQ=255)
                                ttttCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCAtt                                                                 >  1:928311/1‑100 (MQ=255)
                                 tttCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTg                                                                >  1:2464923/1‑100 (MQ=255)
                                  ttCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGa                                                               <  1:546946/100‑1 (MQ=255)
                                   tCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGaa                                                              <  1:649925/100‑1 (MQ=255)
                                   tCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGaa                                                              <  2:560428/100‑1 (MQ=255)
                                     tAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACa                                                            >  2:2605615/1‑100 (MQ=255)
                                     tAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACa                                                            >  1:2590186/1‑100 (MQ=255)
                                       aaaCAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACaaa                                                          <  2:2430028/100‑1 (MQ=255)
                                       aaaCAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACaaa                                                          >  1:1198457/1‑100 (MQ=255)
                                        aaCAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAAt                                                         <  1:1015458/100‑1 (MQ=255)
                                         aCAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACa                                                            >  2:1374394/1‑96 (MQ=255)
                                         aCAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATg                                                        <  2:1353307/100‑1 (MQ=255)
                                         aCAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATg                                                        >  1:893003/1‑100 (MQ=255)
                                         aCAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATg                                                        <  2:1970680/100‑1 (MQ=255)
                                         aCAATACATTCACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCACATCGATAGTTCGTTTACGCCCATTGAACAAATg                                                        <  2:1850932/100‑1 (MQ=255)
                                          cAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGc                                                       >  2:798904/1‑100 (MQ=255)
                                          cAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGc                                                       <  1:602979/100‑1 (MQ=255)
                                          cAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGc                                                       <  1:676680/100‑1 (MQ=255)
                                                aTTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAAt                                                 >  2:725904/1‑100 (MQ=255)
                                                    aCTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTcg                                             <  2:2075460/100‑1 (MQ=255)
                                                     cTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTcgc                                            <  1:1706424/100‑1 (MQ=255)
                                                       ttATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCgcgc                                          >  2:1611751/1‑100 (MQ=255)
                                                        tATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGcc                                         <  2:2072977/100‑1 (MQ=255)
                                                         aTTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCa                                        >  1:75611/1‑100 (MQ=255)
                                                           ttGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAgc                                      >  2:2024905/1‑100 (MQ=255)
                                                            tGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAgcc                                     >  1:2818156/1‑100 (MQ=255)
                                                                aCTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCa                                 <  2:2770548/100‑1 (MQ=255)
                                                                 cTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCAc                                <  1:2576983/100‑1 (MQ=255)
                                                                  tGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACg                               <  1:904377/100‑1 (MQ=255)
                                                                   gTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGa                              >  2:2216882/1‑100 (MQ=255)
                                                                     cGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAg                            <  1:1567507/100‑1 (MQ=255)
                                                                     cGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAg                            >  2:2483775/1‑100 (MQ=255)
                                                                      gTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGa                           >  1:1057979/1‑100 (MQ=255)
                                                                       ttACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGAt                          >  1:1038921/1‑100 (MQ=255)
                                                                       ttACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGAt                          >  2:2780344/1‑100 (MQ=255)
                                                                          cTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGAtttt                       >  1:2471815/1‑100 (MQ=255)
                                                                          cTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGAtttt                       >  2:33986/1‑100 (MQ=255)
                                                                            atatcttcggAAATTAATGAGGCCATACCCAAATTGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTcc                     <  1:1222574/91‑1 (MQ=255)
                                                                            atatCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTcc                     <  2:2316212/100‑1 (MQ=255)
                                                                            atatCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTcc                     <  1:903114/100‑1 (MQ=255)
                                                                            atatCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTcc                     <  2:2464911/100‑1 (MQ=255)
                                                                            atatCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTcc                     <  2:2542589/100‑1 (MQ=255)
                                                                            atatCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTcc                     <  1:10772/100‑1 (MQ=255)
                                                                                   cTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAg              <  2:1402777/100‑1 (MQ=255)
                                                                                   cTGAAATTAATGAGACCATACCCAAATGGATAGTTCGCTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACCAAGaatatcacta                  >  2:2457126/1‑87 (MQ=255)
                                                                                    tGAAATTAATGAGGCCATACCCAAATGGTTAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAgg             <  1:252830/100‑1 (MQ=255)
                                                                                    tGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAgg             >  2:657397/1‑100 (MQ=255)
                                                                                     gAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGga            >  2:1588213/1‑100 (MQ=255)
                                                                                     gAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATCTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGga            >  1:2690589/1‑100 (MQ=255)
                                                                                      aaaTTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGgag           <  1:2780356/100‑1 (MQ=255)
                                                                                      aaaTTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGgag           >  2:429906/1‑100 (MQ=255)
                                                                                      aaaTTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGgag           >  1:1933298/1‑100 (MQ=255)
                                                                                        aTTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGAt         >  2:1475831/1‑100 (MQ=255)
                                                                                          tAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATcc       <  1:590898/100‑1 (MQ=255)
                                                                                          tAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATcc       >  2:1891869/1‑100 (MQ=255)
                                                                                             tGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTc    <  2:1625507/100‑1 (MQ=255)
                                                                                             tGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTc    <  1:2480787/100‑1 (MQ=255)
                                                                                              gAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCt   <  1:2780971/100‑1 (MQ=255)
                                                                                               aGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTg  <  2:2719591/100‑1 (MQ=255)
                                                                                                  |                                                                                                
GTGATGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTG  >  NC_000913/2810662‑2810856

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A