Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A16 F48 I0 R1	252	171.1	8397611	93.1%	7818175	99.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,471,357	C→T	16.3%	intergenic (‑28/+43)	tufA ← / ← fusA	translation elongation factor EF‑Tu 1/protein chain elongation factor EF‑G, GTP‑binding

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,471,357	0	C	T	16.3%	443.1 / 67.0	186	intergenic (‑28/+43)	tufA/fusA	translation elongation factor EF‑Tu 1/protein chain elongation factor EF‑G, GTP‑binding
Reads supporting (aligned to +/- strand): ref base C (82/72); new base T (16/14); total (100/86)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.54e-01

Only 100 of 198 total aligned reads displayed.

ACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATA  >  NC_000913/3471258‑3471456

aCCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTcc                                                                                                     <  1:516445/100‑1 (MQ=255)

 ccGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTccc                                                                                                    <  1:3417525/100‑1 (MQ=255)

 ccGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTccc                                                                                                    >  2:4123205/1‑100 (MQ=255)

 ccGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTccc                                                                                                    >  2:505338/1‑100 (MQ=255)

   gTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCt                                                                                                  >  1:1040363/1‑100 (MQ=255)

        cAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAgg                                                                                             <  1:3040651/100‑1 (MQ=255)

        cAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAgg                                                                                             >  1:1883121/1‑100 (MQ=255)

        cAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCCCTCCCCTTCAgg                                                                                             >  1:501678/1‑100 (MQ=255)

          aCGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGgag                                                                                           >  1:3799011/1‑100 (MQ=255)

          aCGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGgag                                                                                           >  1:73535/1‑100 (MQ=255)

           cGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGgaga                                                                                          >  1:2061370/1‑100 (MQ=255)

              ggCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGagagag                                                                                       >  1:223772/1‑100 (MQ=255)

               gCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCTCCTGAAGGGGAGAGc                                                                                      <  2:2602761/100‑1 (MQ=21)

               gCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCTCCTGAAGGGGAGAGc                                                                                      <  1:1591830/100‑1 (MQ=21)

               gCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCTCCTGAAGGGGAGAGc                                                                                      <  1:2910991/100‑1 (MQ=21)

               gCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCTCCTGAAGGGGAGAGc                                                                                      <  2:3244136/100‑1 (MQ=21)

               gCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGc                                                                                      >  1:3989067/1‑100 (MQ=255)

                 cGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCTCCTGAAGGGGAGAGCAc                                                                                    <  2:3197509/100‑1 (MQ=25)

                  gATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCTCCTGAAGGGGAGAGCAct                                                                                   <  2:1987670/100‑2 (MQ=25)

                   aTAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCTCCTGAAGGGGAGAGCActa                                                                                  <  2:1252613/100‑3 (MQ=25)

                   aTAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCTCCTGAAGGGGAGAGCActa                                                                                  <  2:2239187/100‑3 (MQ=25)

                    tAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCTCCTGAAGGGGAGAGCActat                                                                                 <  1:2573994/100‑4 (MQ=25)

                    tAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACggg                                                                                 <  1:987352/100‑1 (MQ=255)

                    tAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACggg                                                                                 <  2:1798265/100‑1 (MQ=255)

                    tAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACggg                                                                                 <  1:3534531/100‑1 (MQ=255)

                    tAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACggg                                                                                 <  2:1063566/100‑1 (MQ=255)

                      gTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGATAGAGCACGGGAc                                                                               <  1:2762059/100‑1 (MQ=255)

                         ccAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACttt                                                                            >  2:2610614/1‑100 (MQ=255)

                         ccAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACttt                                                                            >  1:1349156/1‑100 (MQ=255)

                             cGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTa                                                                        >  2:2728855/1‑100 (MQ=255)

                             cGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTa                                                                        <  1:1130179/100‑1 (MQ=255)

                             cGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTa                                                                        <  1:1470551/100‑1 (MQ=255)

                             cGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTGACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTa                                                                        <  1:3384321/100‑1 (MQ=255)

                                 aaCGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTaa                                                                    <  1:3435150/100‑1 (MQ=255)

                                 aaCGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACAGGACTTTGGTATTaa                                                                    <  2:3912766/100‑1 (MQ=255)

                                   cGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAAcc                                                                  <  1:699045/100‑1 (MQ=255)

                                   cGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAAcc                                                                  >  1:1122625/1‑100 (MQ=255)

                                    gTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAAcc                                                                  >  2:3136138/1‑99 (MQ=255)

                                      gCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCtt                                                               >  1:2102558/1‑100 (MQ=255)

                                      gCGGTTTTGTACGTTAAAATTTTTCTTTAGACACGGCTTTCTTCGTTACTATAGTGCTTTCGCCTTCAGCAGAGTCTACGGGACTTTGGTTTTAAcccgt                                                               >  2:2644714/1‑98 (MQ=25)

                                       cGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTa                                                              <  2:1275791/100‑1 (MQ=255)

                                        ggTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTGCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAg                                                             >  1:2941570/1‑100 (MQ=255)

                                         gTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAgg                                                            <  2:3480705/100‑1 (MQ=255)

                                           tttGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAACACGGGACTTTGGTATTAACCCTTAGGCt                                                          <  1:3149286/100‑1 (MQ=255)

                                             tGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTa                                                        <  2:2017208/100‑1 (MQ=255)

                                             tGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTa                                                        >  2:1667063/1‑100 (MQ=255)

                                              gTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTACTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTAt                                                       <  1:2296178/100‑1 (MQ=255)

                                                aCGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTAttt                                                     <  2:2934287/100‑1 (MQ=255)

                                                aCGTGCAAATTTTTCTCTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCGGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTAttt                                                     <  2:2443893/100‑1 (MQ=255)

                                                 cGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGATAGCACGGGACTTTGGTATTAATCCTTAGGCTTATTTa                                                    >  2:3661067/1‑100 (MQ=255)

                                                 cGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTAttt                                                     <  2:106489/99‑1 (MQ=255)

                                                  gTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTAc                                                   >  2:798129/1‑100 (MQ=255)

                                                    tCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCa                                                 >  2:3648170/1‑100 (MQ=255)

                                                     cAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTTCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCAc                                                >  2:3729091/1‑100 (MQ=255)

                                                     cAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCAc                                                >  1:682105/1‑100 (MQ=255)

                                                       aaTTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACgg                                              >  1:873936/1‑100 (MQ=255)

                                                            ttCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTc                                         <  2:3989040/100‑1 (MQ=255)

                                                             tCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCa                                        <  2:3798987/100‑1 (MQ=255)

                                                              cTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCaa                                       >  2:1198779/1‑100 (MQ=255)

                                                               tttAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAAt                                      <  1:1798372/100‑1 (MQ=255)

                                                                 tAGACACGGCTCTATTCCTTACTATAGTGCTCTCCCCTTATGGAGAGAGCACGGGACTTGGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAAtt                                     <  2:1488588/99‑1 (MQ=255)

                                                                 tAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTa                                    >  2:3839299/1‑100 (MQ=255)

                                                                   gACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACg                                  <  1:3122231/100‑1 (MQ=255)

                                                                   gACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACg                                  >  1:2485197/1‑100 (MQ=255)

                                                                   gACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACg                                  >  1:552523/1‑100 (MQ=255)

                                                                     cacGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGc                                >  1:1191423/1‑100 (MQ=255)

                                                                      acGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGcc                               <  1:2151850/100‑1 (MQ=255)

                                                                      acGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGcc                               <  2:3061772/100‑1 (MQ=255)

                                                                         gCTATATTCCTTACTCTAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACGTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGa                            <  1:4131697/100‑1 (MQ=255)

                                                                          cTATATTCCTTACTATAGTGCTCTCCCCTTCGGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAg                           <  2:1916396/100‑1 (MQ=255)

                                                                          cTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAg                           >  2:1143858/1‑100 (MQ=255)

                                                                          cTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGAACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAg                           >  1:1058456/1‑100 (MQ=255)

                                                                            atatTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCa                         <  2:1116962/100‑1 (MQ=255)

                                                                              atTCCTTACTATAGTGCTCTCCCCTTCAGGCGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACGACGGGCTTCAATTACAGCCTggaca                         >  2:3377737/1‑94 (MQ=255)

                                                                                   ttACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTGCCACGGGCTTCAATTACGGCCTGAGCAACGTTAc                  <  1:2773059/100‑1 (MQ=255)

                                                                                     aCTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTc                <  2:2841746/100‑1 (MQ=255)

                                                                                     aCTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTc                >  1:2293686/1‑100 (MQ=255)

                                                                                      ctagattGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCg               <  2:3734514/94‑1 (MQ=255)

                                                                                      cTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGGCTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCg               >  2:492398/1‑100 (MQ=255)

                                                                                       tcccgTGCTCTCTCCTGAAGGGGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCgg              >  1:3208889/5‑100 (MQ=37)

                                                                                        cccgTGCTCTCTCCTGAAGGGGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGgc             >  1:3247121/4‑100 (MQ=38)

                                                                                        cccgTGCTCTCTCCTGAAGGGGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGgc             >  2:1038352/4‑100 (MQ=38)

                                                                                        ataGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGgc             >  2:1903000/1‑100 (MQ=255)

                                                                                         taGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGCGCTTCAATTACGGCCTGAGCAACGTTACTcaccg            >  2:184142/1‑96 (MQ=255)

                                                                                         ccgTGCTCTCTCCTGAAGGGGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGgcg            >  1:280041/3‑100 (MQ=38)

                                                                                          cgTGCTCTCTCCTGAAGGGGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACTGCCTGAGCAACGTTACTCGgcgc           >  2:140252/2‑100 (MQ=38)

                                                                                          cgTGCTCTCTCCTGAAGGGGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTcgccgc           >  2:1496190/2‑100 (MQ=38)

                                                                                          cgTGCTCTCTCCTGAAGGGGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGgcgc           >  1:2889877/2‑100 (MQ=38)

                                                                                          cgTGCTCTCTCCTGAAGGGGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGgcgc           >  2:3489810/2‑100 (MQ=38)

                                                                                          cgTGCTCTCTCCTGAAGGGGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGgcgc           >  2:2039712/2‑100 (MQ=38)

                                                                                           gTGCTCTCTCCTGAAGGGGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCt          >  1:1741495/1‑100 (MQ=38)

                                                                                            tGCTCTCTCCTGAAGGGGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCtt         >  1:757955/1‑100 (MQ=255)

                                                                                            tGCTCTCTCCTGAAGGGGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCtt         >  1:265367/1‑100 (MQ=255)

                                                                                             gCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTtc        <  2:143279/100‑1 (MQ=255)

                                                                                             gCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTtc        >  1:1335092/1‑100 (MQ=255)

                                                                                             gCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTtc        <  2:534738/100‑1 (MQ=255)

                                                                                              ctctCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTtca       <  2:3513015/100‑1 (MQ=255)

                                                                                              ctctCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTtc        <  1:4043292/99‑1 (MQ=255)

                                                                                                ctcCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTtcatc     >  2:3011491/1‑100 (MQ=255)

                                                                                                 tcCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTtcatca    >  2:2267140/1‑100 (MQ=255)

ACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATA  >  NC_000913/3471258‑3471456

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A