Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A16 F48 I0 R1	252	171.1	8397611	93.1%	7818175	99.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,471,361	T→G	16.2%	intergenic (‑32/+39)	tufA ← / ← fusA	translation elongation factor EF‑Tu 1/protein chain elongation factor EF‑G, GTP‑binding

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,471,361	0	T	G	16.2%	473.2 / 82.6	194	intergenic (‑32/+39)	tufA/fusA	translation elongation factor EF‑Tu 1/protein chain elongation factor EF‑G, GTP‑binding
Reads supporting (aligned to +/- strand): ref base T (87/75); new base G (17/15); total (104/90)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.69e-01

Only 100 of 194 total aligned reads displayed.

GGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACT  >  NC_000913/3471263‑3471458

ggTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTc                                                                                                  >  1:1443336/1‑100 (MQ=255)

  tCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGGACGTTCAACTTTTTCTTTAGACACGGCTATATTCCTTACTATAGTTCTCTCCCCTTCAg                                                                                                >  2:3650634/1‑100 (MQ=255)

   cAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCCCTCCCCTTCAgg                                                                                               >  1:501678/1‑100 (MQ=255)

     aCGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGgag                                                                                             >  1:73535/1‑100 (MQ=255)

     aCGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGgag                                                                                             <  2:1269427/100‑1 (MQ=255)

     aCGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATATGTCTTTAGACACGGCTATATTCCTTACTATAGCGCTCTCCCCTTCAGgag                                                                                             <  2:1031497/100‑1 (MQ=255)

      cGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGgag                                                                                             >  1:2061556/1‑99 (MQ=255)

       gTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGgaga                                                                                            >  2:1640523/1‑99 (MQ=255)

       gTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTGCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGgagag                                                                                           <  2:1738000/100‑1 (MQ=255)

         ggCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGagagag                                                                                         <  1:1348679/100‑1 (MQ=255)

         ggCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGagagag                                                                                         >  1:223772/1‑100 (MQ=255)

          gCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCTCCTGAAGGGGAGAGc                                                                                        <  1:1591830/100‑1 (MQ=21)

          gCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCTCCTGAAGGGGAGAGc                                                                                        <  2:2602761/100‑1 (MQ=21)

          gCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCTCCTGAAGGGGAGAGc                                                                                        <  2:3244136/100‑1 (MQ=21)

          gCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGc                                                                                        >  1:1068608/1‑100 (MQ=255)

          gCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGc                                                                                        >  1:3989067/1‑100 (MQ=255)

            cGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCTCCTGAAGGGGAGAGCAc                                                                                      <  1:1295590/100‑1 (MQ=25)

            cGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCTCCTGAAGGGGAGAGCAc                                                                                      <  2:3197509/100‑1 (MQ=25)

              aTAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCTCCTGAAGGGGAGAGCActa                                                                                    <  2:2239187/100‑3 (MQ=25)

              aTAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACgg                                                                                    <  1:2904722/100‑1 (MQ=255)

               tAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCTCCTGAAGGGGAGAGCActat                                                                                   <  1:2573994/100‑4 (MQ=25)

               tAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACggg                                                                                   <  2:1063566/100‑1 (MQ=255)

                aGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCTCCTGAAGGGGAGAGCActata                                                                                  <  1:2295693/100‑5 (MQ=25)

                aGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCTCCTGAAGGGGAGAGCActata                                                                                  <  1:3100711/100‑5 (MQ=25)

                aGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGa                                                                                  <  1:3402328/100‑1 (MQ=255)

                 gTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGAc                                                                                 >  2:3509962/1‑100 (MQ=255)

                 gTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGAc                                                                                 >  1:1779008/1‑100 (MQ=255)

                 gTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGAc                                                                                 >  1:3176618/1‑100 (MQ=255)

                    ccAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACttt                                                                              >  2:2610614/1‑100 (MQ=255)

                       aCGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGt                                                                           >  1:2935567/1‑100 (MQ=255)

                        cGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGt                                                                           <  2:3575944/99‑1 (MQ=255)

                        cGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTa                                                                          <  2:110589/100‑1 (MQ=255)

                        cGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTa                                                                          >  2:2728855/1‑100 (MQ=255)

                          ttAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTAtt                                                                        <  1:1152455/100‑1 (MQ=255)

                          ttAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTAtt                                                                        <  2:181053/100‑1 (MQ=255)

                            aaCGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACAGGACTTTGGTATTaa                                                                      <  2:3912766/100‑1 (MQ=255)

                              cGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAAcc                                                                    <  1:699045/100‑1 (MQ=255)

                              cGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAAcc                                                                    <  2:3665567/100‑1 (MQ=255)

                               gTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAAccc                                                                   <  1:1815726/100‑1 (MQ=255)

                               gTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAAcc                                                                    >  2:3136138/1‑99 (MQ=255)

                                 gCGGTTTTGTACGTTAAAATTTTTCTTTAGACACGGCTTTCTTCGTTACTATAGTGCTTTCGCCTTCAGCAGAGTCTACGGGACTTTGGTTTTAAcccgt                                                                 >  2:2644714/1‑98 (MQ=25)

                                  cGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTa                                                                >  2:1946967/1‑100 (MQ=255)

                                        tGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTa                                                          <  2:2008564/100‑1 (MQ=255)

                                        tGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTa                                                          <  2:2017208/100‑1 (MQ=255)

                                           aCGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTAttt                                                       <  2:2934287/100‑1 (MQ=255)

                                           aCGTGCAAATTTTTCTCTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCGGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTAttt                                                       <  2:2443893/100‑1 (MQ=255)

                                            cGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTa                                                      <  1:3504284/100‑1 (MQ=255)

                                            cGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTa                                                      >  2:3484753/1‑100 (MQ=255)

                                             gTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTAc                                                     >  2:798129/1‑100 (MQ=255)

                                                cAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCAc                                                  >  1:682105/1‑100 (MQ=255)

                                                  aaTTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACgg                                                >  2:104478/1‑100 (MQ=255)

                                                  aaTTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACgg                                                >  1:873936/1‑100 (MQ=255)

                                                      tttCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCtt                                            >  2:3733131/1‑100 (MQ=255)

                                                       ttCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTc                                           <  2:3989040/100‑1 (MQ=255)

                                                         cTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCaa                                         >  1:3662570/1‑100 (MQ=255)

                                                         cTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCaa                                         >  2:1198779/1‑100 (MQ=255)

                                                         cTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCaa                                         >  1:295340/1‑100 (MQ=255)

                                                              gACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACg                                    >  2:1394400/1‑100 (MQ=255)

                                                              gACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACg                                    <  2:1913438/100‑1 (MQ=255)

                                                                cacGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGc                                  >  1:27661/1‑100 (MQ=255)

                                                                 acGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGcc                                 <  1:2151850/100‑1 (MQ=255)

                                                                 acGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGcc                                 <  2:3061772/100‑1 (MQ=255)

                                                                   ggCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTg                               >  1:3128632/1‑100 (MQ=255)

                                                                    gCTATATTCCTTACTCTAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACGTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGa                              <  1:4131697/100‑1 (MQ=255)

                                                                    gCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGa                              <  1:2434563/100‑1 (MQ=255)

                                                                     cTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAg                             <  2:1412257/100‑1 (MQ=255)

                                                                     cTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAg                             >  2:1143858/1‑100 (MQ=255)

                                                                     cTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAg                             <  1:3420350/100‑1 (MQ=255)

                                                                     cTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGAACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAg                             >  1:1058456/1‑100 (MQ=255)

                                                                      tataTTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGc                            >  1:1124019/1‑100 (MQ=255)

                                                                         atTCCTTACTATAGTGCTCTCCCCTTCAGGCGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACGACGGGCTTCAATTACAGCCTggaca                           >  2:3377737/1‑94 (MQ=255)

                                                                         atTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAAc                         >  2:3750840/1‑100 (MQ=255)

                                                                          ttCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACg                        <  2:53090/100‑1 (MQ=255)

                                                                             cTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGtt                      >  2:3799271/1‑99 (MQ=255)

                                                                             cTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTa                     >  2:1870897/1‑100 (MQ=255)

                                                                                aCTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTc                  >  1:2293686/1‑100 (MQ=255)

                                                                                aCTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTc                  <  2:2841746/100‑1 (MQ=255)

                                                                                 cTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGGCTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCg                 >  2:492398/1‑100 (MQ=255)

                                                                                 cTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCg                 >  1:3271659/1‑100 (MQ=255)

                                                                                   cccgTGCTCTCTCCTGAAGGGGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGgc               >  1:2775656/4‑100 (MQ=38)

                                                                                    taGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGgcg              <  1:980530/100‑1 (MQ=255)

                                                                                     cgTGCTCTCTCCTGAAGGGGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGgcgc             >  1:3816939/2‑100 (MQ=38)

                                                                                     cgTGCTCTCTCCTGAAGGGGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGgcgc             >  2:2039712/2‑100 (MQ=38)

                                                                                     cgTGCTCTCTCCTGAAGGGGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATCTACCACGGGCTTCAATTACGGCCTGAGCACCGTTACGCGgagc             >  2:1489394/2‑100 (MQ=37)

                                                                                      gTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCt            <  2:1477338/100‑1 (MQ=255)

                                                                                       tGCTCTCTCCTGAAGGGGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCtt           >  1:265367/1‑100 (MQ=255)

                                                                                       tGCTCTCTCCTGAAGGGGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCtt           >  1:757955/1‑100 (MQ=255)

                                                                                       tGCTCTCTCCTGAAGGGGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTAAGCAACGTTACTCGGCGCtt           >  1:2868817/1‑100 (MQ=255)

                                                                                        gCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTtc          >  1:2035885/1‑100 (MQ=255)

                                                                                        gCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTtc          >  1:3222983/1‑100 (MQ=255)

                                                                                         ctctCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTtc          <  1:4043292/99‑1 (MQ=255)

                                                                                          tctcCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTtcat        <  2:3399808/100‑1 (MQ=255)

                                                                                           ctcCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTtcatc       >  1:527073/1‑100 (MQ=255)

                                                                                           ctcCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTtcatc       <  1:2544897/100‑1 (MQ=255)

                                                                                           ctcCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTtcatc       >  2:3011491/1‑100 (MQ=255)

                                                                                            tcCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTtcatca      >  2:2267140/1‑100 (MQ=255)

                                                                                              cccTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATa    >  1:212329/1‑100 (MQ=255)

                                                                                               ccTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATAc   <  1:1708992/100‑1 (MQ=255)

                                                                                               ccTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATAc   <  2:4118109/100‑1 (MQ=255)

                                                                                               ccTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATAc   <  2:682104/100‑1 (MQ=255)

GGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACT  >  NC_000913/3471263‑3471458

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A