Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A16 F48 I0 R1	252	171.1	8397611	93.1%	7818175	99.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,471,362	C→A	16.2%	intergenic (‑33/+38)	tufA ← / ← fusA	translation elongation factor EF‑Tu 1/protein chain elongation factor EF‑G, GTP‑binding

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,471,362	0	C	A	16.2%	518.8 / 74.7	192	intergenic (‑33/+38)	tufA/fusA	translation elongation factor EF‑Tu 1/protein chain elongation factor EF‑G, GTP‑binding
Reads supporting (aligned to +/- strand): ref base C (85/74); new base A (16/15); total (102/90)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.93e-01

Only 100 of 198 total aligned reads displayed.

GGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCA  >  NC_000913/3471263‑3471461

ggTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTCCGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTCCTATAGTGCTCTCCCCTTc                                                                                                     >  2:2915326/1‑100 (MQ=255)

ggTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTc                                                                                                     >  1:1443336/1‑100 (MQ=255)

  tCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGGACGTTCAACTTTTTCTTTAGACACGGCTATATTCCTTACTATAGTTCTCTCCCCTTCAg                                                                                                   >  2:3650634/1‑100 (MQ=255)

   cAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAgg                                                                                                  <  1:3040651/100‑1 (MQ=255)

    aaCGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGga                                                                                                 <  2:2599274/100‑1 (MQ=255)

     aCGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGgag                                                                                                >  1:2624723/1‑100 (MQ=255)

     aCGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGgag                                                                                                >  1:73535/1‑100 (MQ=255)

     aCGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGgag                                                                                                <  2:1269427/100‑1 (MQ=255)

     aCGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATATGTCTTTAGACACGGCTATATTCCTTACTATAGCGCTCTCCCCTTCAGgag                                                                                                <  2:1031497/100‑1 (MQ=255)

      cGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGgag                                                                                                >  1:2061556/1‑99 (MQ=255)

       gTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGgaga                                                                                               >  2:1640523/1‑99 (MQ=255)

       gTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTGCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGgagag                                                                                              <  2:1738000/100‑1 (MQ=255)

         ggCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGagagag                                                                                            <  1:1348679/100‑1 (MQ=255)

         ggCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGagagag                                                                                            >  1:223772/1‑100 (MQ=255)

          gCCGCTAGTACCTACGTTAACGTGCGGTGTTGTACGTTCAGATTTGTCTTTATACACGGCTATATTCCTTACTATAGTGCTCTCTCCTGAAGGGGAGAGc                                                                                           <  2:2633774/100‑1 (MQ=21)

          gCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCTCCTGAAGGGGAGAGc                                                                                           <  1:1591830/100‑1 (MQ=21)

          gCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCTCCTGAAGGGGAGAGc                                                                                           <  1:2910991/100‑1 (MQ=21)

          gCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGc                                                                                           >  1:3989067/1‑100 (MQ=255)

          gCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGc                                                                                           >  1:1068608/1‑100 (MQ=255)

            cGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCTCCTGAAGGGGAGAGCAc                                                                                         <  1:1295590/100‑1 (MQ=25)

             gATAGTACCAACGTTAACGTGCGGTTTTGTCCGGTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGCAGAGAGCACg                                                                                        >  2:2762052/1‑100 (MQ=255)

               tcgTACCAACGTTAACGTGCGGTTTTGTACTTTCAAATTTTTCTTTAGACACGGCTATATTCCTTGCTATATTGCTCTCCCCTTCAGGAGAGAGCACggg                                                                                      <  2:2607713/98‑1 (MQ=37)

               tAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACggg                                                                                      <  1:3534531/100‑1 (MQ=255)

               tAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACggg                                                                                      <  2:1063566/100‑1 (MQ=255)

                aGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGa                                                                                     <  1:3402328/100‑1 (MQ=255)

                 gTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGATAGAGCACGGGAc                                                                                    <  1:2762059/100‑1 (MQ=255)

                 gTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGAc                                                                                    >  1:1779008/1‑100 (MQ=255)

                 gTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGAc                                                                                    >  2:3509962/1‑100 (MQ=255)

                  tACCAACGTTAACGTGCGGTTTACTACGTTCAATTTTTTCTTTAGACACGGCTATGTACCTTACTATAGTGCTCTCATCTTGCGGAGAGAGTAcagaac                                                                                    >  2:2934854/1‑94 (MQ=21)

                        cGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGt                                                                              <  2:3575944/99‑1 (MQ=255)

                        cGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTa                                                                             <  2:110589/100‑1 (MQ=255)

                          ttAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTAtt                                                                           <  1:1152455/100‑1 (MQ=255)

                          ttAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTAtt                                                                           <  2:181053/100‑1 (MQ=255)

                            aaCGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTaa                                                                         <  1:3435150/100‑1 (MQ=255)

                            aaCGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACAGGACTTTGGTATTaa                                                                         <  2:3912766/100‑1 (MQ=255)

                             aCGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAAc                                                                        <  1:690044/100‑1 (MQ=255)

                             aCGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAAc                                                                        >  2:2651068/1‑100 (MQ=255)

                              cGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAAcc                                                                       <  2:3665567/100‑1 (MQ=255)

                               gTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAAccc                                                                      <  1:1815726/100‑1 (MQ=255)

                               gTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAAccc                                                                      >  2:3324396/1‑100 (MQ=255)

                                  cGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTa                                                                   >  2:1946967/1‑100 (MQ=255)

                                   ggTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTGCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAg                                                                  >  1:2941570/1‑100 (MQ=255)

                                      tttGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAACACGGGACTTTGGTATTAACCCTTAGGCt                                                               <  1:3149286/100‑1 (MQ=255)

                                        tGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTa                                                             >  2:2257953/1‑100 (MQ=255)

                                        tGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTa                                                             <  2:2008564/100‑1 (MQ=255)

                                         gTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTACTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTAt                                                            <  1:2296178/100‑1 (MQ=255)

                                            cGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTa                                                         >  2:3484753/1‑100 (MQ=255)

                                             gTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTAc                                                        >  2:798129/1‑100 (MQ=255)

                                                  aaTTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACgg                                                   >  1:873936/1‑100 (MQ=255)

                                                    tttttCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTGCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGc                                                 <  1:515411/100‑1 (MQ=255)

                                                     ttttttttCGACACGGCTATGTTCCTTACTCTAGTGCTCTCCCCGTGTGCAGATAGAACGGGACGTCGTTATTAACCCTTAGGCTTATTTACCACGGGCt                                                <  2:3091265/100‑1 (MQ=255)

                                                      tttCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCtt                                               >  2:3733131/1‑100 (MQ=255)

                                                       ttCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTc                                              <  2:3989040/100‑1 (MQ=255)

                                                         cTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCaa                                            >  1:3662570/1‑100 (MQ=255)

                                                         cTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCaa                                            >  2:1198779/1‑100 (MQ=255)

                                                              gACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACg                                       <  2:1913438/100‑1 (MQ=255)

                                                              gACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACg                                       >  1:2485197/1‑100 (MQ=255)

                                                              gACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACg                                       >  2:1394400/1‑100 (MQ=255)

                                                              gACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACg                                       <  1:3122231/100‑1 (MQ=255)

                                                              gACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACg                                       >  1:552523/1‑100 (MQ=255)

                                                                 acGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGcc                                    <  1:2151850/100‑1 (MQ=255)

                                                                    gCTATATTCCTTACTCTAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACGTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGa                                 <  1:4131697/100‑1 (MQ=255)

                                                                     cTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAg                                <  2:1412257/100‑1 (MQ=255)

                                                                     cTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAg                                >  2:2030009/1‑100 (MQ=255)

                                                                     cTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAg                                >  2:1143858/1‑100 (MQ=255)

                                                                     cTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGAACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAg                                >  1:1058456/1‑100 (MQ=255)

                                                                      tataTTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGc                               >  1:1124019/1‑100 (MQ=255)

                                                                         atTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAAc                            >  2:3750840/1‑100 (MQ=255)

                                                                         atTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAAc                            >  1:496440/1‑100 (MQ=255)

                                                                          ttCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACg                           <  2:3025586/100‑1 (MQ=255)

                                                                          ttCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACg                           <  2:53090/100‑1 (MQ=255)

                                                                             cTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGtt                         >  2:3799271/1‑99 (MQ=255)

                                                                             cTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTa                        >  2:1870897/1‑100 (MQ=255)

                                                                              ttACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTACCCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTAc                       >  2:2069876/1‑100 (MQ=255)

                                                                              ttACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTGCCACGGGCTTCAATTACGGCCTGAGCAACGTTAc                       <  1:2773059/100‑1 (MQ=255)

                                                                                aCTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTc                     >  1:2293686/1‑100 (MQ=255)

                                                                                aCTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTCTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTc                     <  2:2279757/100‑1 (MQ=255)

                                                                                 cTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGGCTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCg                    >  2:492398/1‑100 (MQ=255)

                                                                                 cTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCg                    >  1:3271659/1‑100 (MQ=255)

                                                                                   cccgTGCTCTCTCCTGAAGGGGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGgc                  >  2:1038352/4‑100 (MQ=38)

                                                                                   ataGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGgc                  >  2:3207734/1‑100 (MQ=255)

                                                                                    taGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGgcg                 <  1:980530/100‑1 (MQ=255)

                                                                                    ccgTGCTCTCTCCTGAAGGGGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGgcg                 >  1:280041/3‑100 (MQ=38)

                                                                                     cgTGCTCTCTCCTGAAGGGGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGgcgc                >  1:3816939/2‑100 (MQ=38)

                                                                                     cgTGCTCTCTCCTGAAGGGGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGgcgc                >  1:2889877/2‑100 (MQ=38)

                                                                                     cgTGCTCTCTCCTGAAGGGGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATCTACCACGGGCTTCAATTACGGCCTGAGCACCGTTACGCGgagc                >  2:1489394/2‑100 (MQ=37)

                                                                                      gTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCt               <  2:1477338/100‑1 (MQ=255)

                                                                                       tGCTCTCTCCTGAAGGGGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCtt              >  1:757955/1‑100 (MQ=255)

                                                                                        gCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTtc             >  1:2035885/1‑100 (MQ=255)

                                                                                        gCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTtc             >  1:3222983/1‑100 (MQ=255)

                                                                                         ctctCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTtc             <  1:4043292/99‑1 (MQ=255)

                                                                                          tctcCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTtcat           <  2:3399808/100‑1 (MQ=255)

                                                                                             ccccTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTcatcat        >  2:3156300/1‑100 (MQ=255)

                                                                                              cccTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATa       >  1:212329/1‑100 (MQ=255)

                                                                                               ccTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATAc      <  1:1708992/100‑1 (MQ=255)

                                                                                               ccTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATAc      <  2:4118109/100‑1 (MQ=255)

                                                                                               ccTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATAc      <  2:682104/100‑1 (MQ=255)

                                                                                               ccTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATAc      >  1:256819/1‑100 (MQ=255)

                                                                                                   cAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCa  <  1:3180426/100‑1 (MQ=255)

                                                                                                   cAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCa  <  1:722665/100‑1 (MQ=255)

GGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCA  >  NC_000913/3471263‑3471461

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A