Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A16 F48 I0 R1 252 171.1 8397611 93.1% 7818175 99.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,051,262 A→C 20.0% intergenic (‑497/‑85) yihA ← / → yihI cell division GTP‑binding protein/activator of Der GTPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,051,2620AC20.0% 438.2 / 111.4 194intergenic (‑497/‑85)yihA/yihIcell division GTP‑binding protein/activator of Der GTPase
Reads supporting (aligned to +/- strand):  ref base A (85/70);  new base C (21/18);  total (106/88)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.02e-01

Only 100 of 199 total aligned reads displayed.

GGATTGTTCCGGCTAAAGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCATTATGAAACCATCATCT  >  NC_000913/4051163‑4051361
                                                                                                   |                                                                                                   
  aTTGTTCCGGCTAAAGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATc                                                                                                   >  1:1234016/1‑100 (MQ=255)
       tCCGGCTAAAGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCg                                                                                              <  1:2445431/100‑1 (MQ=255)
       tCCGGCTAAAGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCg                                                                                              <  2:2978860/100‑1 (MQ=255)
        ccGGCTAAAGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGc                                                                                             <  2:2934776/100‑1 (MQ=255)
        ccGGCTAAAGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGAACCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGGAATCTGt                                                                                                >  2:3588545/1‑97 (MQ=255)
         cGGCTAAAGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGATTACTCTGTCGcc                                                                                            <  1:3597347/100‑1 (MQ=255)
         cGGCTAAAGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGATTACTCTGTCGcc                                                                                            <  1:3931376/100‑1 (MQ=255)
         cGGCTAAAGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGATTACTCTGTCGcc                                                                                            <  1:443963/100‑1 (MQ=255)
         cGGCTAAAGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGcc                                                                                            <  1:1869804/100‑1 (MQ=255)
          ggCTAAAGGAAAAACAGGGTGTGTTGGTGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGATTACTCTGTCGCCt                                                                                           <  2:2418543/100‑1 (MQ=38)
           gCTAAAGGAAAAACAGGGTGTGTTGGCGGTTTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGATTACTCTGTCGCCtt                                                                                          <  1:3689202/100‑1 (MQ=38)
           gCTAAAGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGATTACTCTGTCGCCtt                                                                                          <  1:163882/100‑1 (MQ=255)
           gCTAAAGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGATTACTCTGTCGCCtt                                                                                          <  1:1130321/100‑1 (MQ=255)
            cTAAAGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGATTACTCTGTCGCCttt                                                                                         <  1:1921652/100‑1 (MQ=255)
             tAAAGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCtttt                                                                                        >  2:1894821/1‑100 (MQ=255)
             naaaGGAAAAACAGGGTGTGTTGGCGGCCTGCCAGGCTTGTAAGACCCGTTAAGGGTAATGAGTCCGGCAAAAAGGCGACAAAGCAATCTGTCGCCtt                                                                                          >  2:1619858/2‑98 (MQ=255)
              aaaGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCttttt                                                                                       >  2:3074999/1‑100 (MQ=255)
              aaaGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCttttt                                                                                       >  1:4111164/1‑100 (MQ=255)
               aaGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCtttttt                                                                                      >  2:2719358/1‑100 (MQ=255)
               aaGGAAAAACAGGGGGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAAGAAAAAAGGCGACAGATTACTCTGTCGCCtt                                                                                          <  2:3411338/96‑1 (MQ=38)
                aGGAAAAACGGGGTGTGTTGGCGGCCTGCAAGGAGTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATGTGTCGCCTTTTTTc                                                                                     >  2:3960658/1‑100 (MQ=255)
                aGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGATTACTCTGTCGCCTTTTTTc                                                                                     <  1:3667354/100‑1 (MQ=255)
                aGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTc                                                                                     >  1:3102890/1‑100 (MQ=255)
                aGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTc                                                                                     >  2:3729547/1‑100 (MQ=255)
                 ggAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCt                                                                                    >  1:2592377/1‑100 (MQ=255)
                  gAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGATTACTCTGTCGCCTTTTTTcct                                                                                   <  2:1671846/100‑3 (MQ=255)
                   aaaaaCAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCttt                                                                                  >  1:823886/1‑100 (MQ=255)
                    aaaaCAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTttg                                                                                 <  1:2327407/100‑1 (MQ=255)
                      aaCAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTttgct                                                                               <  2:3531887/100‑1 (MQ=255)
                      aaCAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTttgct                                                                               <  1:1026373/100‑1 (MQ=255)
                         aGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGCGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTttgcttgc                                                                            <  2:4181158/100‑1 (MQ=255)
                           ggTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTgcttgctt                                                                          <  1:3662228/100‑1 (MQ=255)
                               tgtTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTg                                                                      <  1:943898/100‑1 (MQ=255)
                                gtTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGt                                                                     <  1:2272511/100‑1 (MQ=255)
                                  tGGCGGCCTGTAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGTTTTCTGTTa                                                                   <  2:905473/100‑1 (MQ=255)
                                  tGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTa                                                                   <  1:2564911/100‑1 (MQ=255)
                                  tGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTa                                                                   >  1:2854942/1‑100 (MQ=255)
                                  tGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTa                                                                   >  1:2134217/1‑100 (MQ=255)
                                   ggcggcCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTa                                                                   <  2:3132941/99‑1 (MQ=255)
                                     ctgcCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGATTCAGGTAAAAAGGCGACTGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAg                                                                  <  2:1864162/96‑1 (MQ=39)
                                         cTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTccg                                                            >  1:3456953/1‑100 (MQ=255)
                                          tGCACGGATTGTAAGACCCGTTCAGGGTCATCAGTCAGGAAACAACGCGACACAGTAATCTGTCGCCTTTTCTCCTTGCCTGCTTTCTGTTAGATTcct                                                            >  2:861950/1‑98 (MQ=255)
                                          tGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTccgc                                                           >  1:3000591/1‑100 (MQ=255)
                                           gCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTccgcc                                                          >  2:4123108/1‑100 (MQ=255)
                                           gCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTccgcc                                                          >  1:3992437/1‑100 (MQ=255)
                                           gCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTccgcc                                                          >  1:1783757/1‑100 (MQ=255)
                                            cAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTccgccg                                                         >  1:1725635/1‑100 (MQ=255)
                                               ggATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCaa                                                      >  1:3538898/1‑100 (MQ=255)
                                               ggATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCaa                                                      >  1:3814736/1‑100 (MQ=255)
                                                  ttGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATc                                                   <  1:2905611/100‑1 (MQ=255)
                                                  ttGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATc                                                   <  1:97277/100‑1 (MQ=255)
                                                  ttGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATc                                                   >  1:3220136/1‑100 (MQ=255)
                                                    gTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCta                                                 >  1:2670893/1‑100 (MQ=255)
                                                    gTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCta                                                 >  2:1978940/1‑100 (MQ=255)
                                                     tAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGCCAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATGta                                                 >  2:1689636/1‑99 (MQ=255)
                                                     tAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCtat                                                <  1:2264759/100‑1 (MQ=255)
                                                       aGACCTGTTAAGGGTTATGAGTTAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCtat                                                <  2:638077/98‑1 (MQ=255)
                                                       aGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCGTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCtata                                               >  2:2830669/1‑99 (MQ=255)
                                                       aGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATAc                                              <  2:1998886/100‑1 (MQ=255)
                                                       aGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATAc                                              <  1:3135448/100‑1 (MQ=255)
                                                         aCCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTg                                            >  1:1956214/1‑100 (MQ=255)
                                                         aCCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTg                                            >  2:1107047/1‑100 (MQ=255)
                                                          cccGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGa                                           >  1:1554129/1‑100 (MQ=255)
                                                            cGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAAt                                         >  1:3230181/1‑100 (MQ=255)
                                                             gTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATa                                        <  1:1014845/100‑1 (MQ=255)
                                                              ttAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATa                                        <  2:1922965/99‑1 (MQ=255)
                                                                  gggTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGaaagatcgg                                   >  2:2873249/1‑93 (MQ=255)
                                                                   ggTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACgg                                  >  1:2817688/1‑100 (MQ=255)
                                                                   ggTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACgg                                  <  1:4063452/100‑1 (MQ=255)
                                                                    gTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGc                                 <  1:1359745/100‑1 (MQ=255)
                                                                        caagcaaagAAAAAAGGCGACAGATTACTCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATACAACg                                   >  2:4075471/9‑94 (MQ=21)
                                                                          aGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAg                           <  1:2291824/100‑1 (MQ=255)
                                                                           gTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGACTAAAACGGCTAAAAg                           >  2:1649093/1‑99 (MQ=255)
                                                                           gTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGcagatcgg                          >  1:1086567/1‑93 (MQ=255)
                                                                           gTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGa                          >  1:2800821/1‑100 (MQ=255)
                                                                            caaagAAAAAAGGCGACAGATTACTCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGACGCAAATCTATACTGAATAAAACGCATAAAacac                         >  2:1266521/5‑100 (MQ=25)
                                                                             cAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAATGGCTAAAAGACg                        >  2:3480665/1‑100 (MQ=255)
                                                                             cAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACg                        >  1:3348661/1‑100 (MQ=255)
                                                                               ggAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGaa                      >  1:764019/1‑100 (MQ=255)
                                                                                    aaaGGCGACAGATTACTCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCAtt                 >  1:3624970/1‑100 (MQ=255)
                                                                                     aaGGCGACAGAGTAATCTGTCGCCTTTTTTTTTTTCTTTCTTTCTGTTAGATTCCGCCGCAACTCTATACTGAATCACACGGCTAAAAGACGAAc                     >  2:1030532/1‑95 (MQ=255)
                                                                                      aGGCGACAGAGTAATCTGTCTCCTTTTTTCTTTGCTTGTTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCATTAt               <  2:1171112/100‑1 (MQ=255)
                                                                                       ggCGACAGATTACTCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCATTATg              >  1:3299072/1‑100 (MQ=255)
                                                                                       ggCGACAGATTACTCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCATTATg              >  1:3567879/1‑100 (MQ=255)
                                                                                       ggCGACAGATTACTCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCATTATg              >  1:1422921/1‑100 (MQ=255)
                                                                                       ggCGACAGATTACTCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCATTATg              >  1:951542/1‑100 (MQ=255)
                                                                                       ggCGACAGATTACTCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCATTATg              >  1:274890/1‑100 (MQ=255)
                                                                                         cGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCATTATGaa            >  1:3945434/1‑100 (MQ=255)
                                                                                         cGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCATTATGaa            <  2:2099549/100‑1 (MQ=255)
                                                                                            cAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCATTATGAAAcc         <  1:2060661/100‑1 (MQ=255)
                                                                                             agagTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCATTATGAAACca        <  2:226047/100‑1 (MQ=255)
                                                                                              gagTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCATTATGAAACcat       >  2:58702/1‑100 (MQ=255)
                                                                                               agTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCATTATGAAACcatc      <  2:2813096/100‑1 (MQ=255)
                                                                                               agTAATCTGTCGCCTTTTTTCTGTGCTTGCTTTCTGATAGATACCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCATTATGAAACcat       <  2:1455133/99‑1 (MQ=255)
                                                                                                gTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCATTATGAAACcatca     <  2:3344846/100‑1 (MQ=255)
                                                                                                gTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCATTATGAAACcatca     <  1:1615533/100‑1 (MQ=255)
                                                                                                gTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCATTATGAAACcatca     <  1:1490140/100‑1 (MQ=255)
                                                                                                gTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCATTATGAAACcatca     <  2:1509049/100‑1 (MQ=255)
                                                                                                  aaTCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCATTATGAAACCatcatc   <  1:1472596/100‑1 (MQ=255)
                                                                                                   aTCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCATTATGAAACCATCATCt  <  1:924437/100‑1 (MQ=255)
                                                                                                   |                                                                                                   
GGATTGTTCCGGCTAAAGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCATTATGAAACCATCATCT  >  NC_000913/4051163‑4051361

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A