Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A10 F44 I1 R1
|
19 |
65.8 |
3201541 |
96.2% |
3079882 |
99.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,955,062 |
A→T |
D314E (GAT→GAA) |
recB ← |
exonuclease V (RecBCD complex), beta subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,955,062 | 0 | A | T | 98.3%
| 207.0
/ NA
| 60 | D314E (GAT→GAA) | recB | exonuclease V (RecBCD complex), beta subunit |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base T (33/26); minor base C (0/1); total (33/27) |
| Fisher's exact test for biased strand distribution p-value = 4.50e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CACGGCGGCGTTTTTCACGCGCTACTGTTTCGCGGATCTCAGCCAATGCGCGGGTGATCACCAGATCGCGGATCGACAATGGTTCTGCAAGCAGTTGATCGATCGCCTCAAACAGTGGATGTCGCGGGGTTTCCCCCCCGGCCTTCGTGCGATCTTCTAAGAAACGCTGGGAGAATTTTTCCAGCGACTCCGG > NC_000913/2954965‑2955157
|
cACGGCGGCGTTTTTCACGCGCTACTGTTTCGCGGATCTCAGCCAATGCGCGGGTGATCACCAGATCGCGGATCGACAATGGTTCTGCAAGCAGTTgttc < 1:1038114/100‑1 (MQ=255)
ggcggcGTTTTTCACGCGCTACTGTTTCGCGGATCTCAGCCAATGCGCGGGTGATCACCAGATCGCGGATCGACAATGGTTCTGCAAGCAGTGgttcgat < 2:840341/100‑1 (MQ=255)
gcgTTTTTCACGCGCTACTGTTTCGCGGATCTCAGCCAATGCGCGGGTGATCACCAGATCGCGGATCGACAATGGTTCTGCAAGCAGTTGTTCGATCGcc < 2:142665/100‑1 (MQ=255)
gcgTTTTTCACGCGCTACTGTTTCGCGGATCTCAGCCAATGCGCGGGTGATCACCAGATCGCGGATCGACAATGGTTCTGCAAGCAGTTGTTCGATCGcc > 1:1303565/1‑100 (MQ=255)
cgTTTTTCACGCGCTACTGTTTCGCGGATCTCAGCCAATGCGCGGGTGATCACCAGATCGCGGATCGACAATGGTTCTGCAAGCAGTTGTTCGATCGCCt > 1:355089/1‑100 (MQ=255)
ttCACGCGCTACTGTTTCGCGGATCTCAGCCAATGCGCGGGTGATCACCAGATCGCGGATCGACAATGGTTCTGCAAGCAGTTGTTCGATCGCCTCAAAc > 1:352755/1‑100 (MQ=255)
ttCACGCGCTACTGTTTCGCGGATCTCAGCCAATGCGCGGGTGATCACCAGATCGCGGATCGACAATGGTTCTGCAAGCAGTTGTTCGATCGCCTCAAAc < 2:1174322/100‑1 (MQ=255)
cgcgCTACTGTTTCGCGGATCTCAGCCAATGCGCGGGTGATCACCAGATCGCGGATCGACAATGGTTCTGCAAGCAGTTGTTCGATCGCCTCAAACAGTg > 1:1425094/1‑100 (MQ=255)
cgcgCTACTGTTTCGCGGATCTCAGCCAATGCGCGGGTGATCACCAGATCGCGGATCGACAATGGTTCTGCAAGCAGTTGTTCGATCGCCTCAAACAGTg > 2:249/1‑100 (MQ=255)
cgcTACTGTTTCGCGGATCTCAGCCAATGCGCGGGTGATCACCAGATCGCGGATCGACAATGGTTCTGCAAGCAGTTGTTCGATCGCCTCAAACAGTGGa > 2:413443/1‑100 (MQ=255)
aCTGTTTCGCGGATCTCAGCCAATGCGCGGGTGATCACCAGATCGCGGATCGACAATGGTTCTGCAAGCAGTTGTTCGATCGCCTCAAACAGTGGATGTc > 2:1383847/1‑100 (MQ=255)
cTGTTTCGCGGATCTCAGCCAATGCGCGGGTGATCACCAGATCGCGGATCGACAATGGTTCTGCAAGCAGTTGTTCGATCGCCTCAAACAGTGGATGTcg > 2:315711/1‑100 (MQ=255)
tCGCGGATCTCAGCCAATGCGCGGGTGATCACCAGATCGCGGATCGACAATGGTTCTGCAAGCAGTTGTTCGATCGCCTCAAACAGTGGATGTCGCgggg > 1:1329379/1‑100 (MQ=255)
cgcgGATCTCAGCCAATGCGCGGGTGATCACCAGATCGCGGATCGACAATGGTTCTGCAAGTAGTTGTTCGATCGCCTCAAACAGTGGATGTCGCGGGGt < 1:984886/100‑1 (MQ=255)
cgcgGATCTCAGCCAATGCGCGGGTGATCACCAGATCGCGGATCGACAATGGTTCTGCAAGCAGTTGTTCGATCGCCTCAAACAGTGGATGTCGCGGGGt > 2:509377/1‑100 (MQ=255)
cgGATCTCAGCCAATGCGCGGGTGATCGCCAGATCGCGGATCGACAATGGTTCTGCAAGCAGTTGTTCGATCGCCTCAAACAGTGGATGTCGAGGGGttt < 1:521508/100‑1 (MQ=255)
ggATCTCAGCCAATGCGCGGGTGATCACCAGATCGCGGATCGACAATGGTTCTGCAAGCAGTTGTTCGATCGCCTCAAACAGTGGATGTCGCGGGGTTTc < 2:497206/100‑1 (MQ=255)
ggATCTCAGCCAATGCGCGGGTGATCACCAGATCGCGGATCGACAATGGTTCTGCAAGCAGTTGTTCGATCGCCTCAAACAGTGGATGTCGCGGGGTTTc > 2:844394/1‑100 (MQ=255)
gATCTCAGCCAATGCTCGGGTGATCACCAGATCGCGGATCGACAATGGTTCTGCAAGCAGTTGTTCGATCGCCTCAAACAGTGGATGTCGCGGGGTTTcc < 2:1578084/100‑1 (MQ=255)
gATCTCAGCCAATGCGCGGGTGATCACCAGATCGCGGATCGACAATGGTTCTGCAAGCAGTTGTTCGATCGCCTCAAACAGTGGATGTCGCGGGGTTTcc > 2:1047154/1‑100 (MQ=255)
gATCTCAGCCAATGCGCGGGTGATCACCAGATCGCGGATCGACAATGGTTCTGCAAGCAGTTGTTCGATCGCCTCAAACAGTGGATGTCGCGGGGTTTcc < 1:1383833/100‑1 (MQ=255)
aTCTCAGCCAATGCGCGGGTGATCACCAGATCGCGGATCGACAATGGTTCTGCAAGCAGTTGTTCGATCGCCTCAAACAGTGGATGTCGCGGGGTTTccc > 2:1063021/1‑100 (MQ=255)
tctcAGCCAATGCGCGGGTGATCACCAGATCGCGGATCGACAATGGTTCTGCAAGCAGTTGTTCGATCGCCTCAAACAGTGGATGTCGCGGGGTTTcccc < 2:607466/100‑1 (MQ=255)
tcAGCCAATGCGCGGGTGATCACCAGATCGCGGATCGACAATGGTTCTGCAAGCAGTTGTTCGATCGCCTCAAACAGTGGATGTCGCGGGGTTTcccccc > 2:540479/1‑100 (MQ=255)
cAGCCAATGCGCGGGTGATCACCAGATCGCGGATCGACAATGGTTCTGCAAGCAGTTGTTCGATCGCCTCAAACAGTGGATGTCGCGGGGTTTccccccc > 1:221116/1‑100 (MQ=255)
cAGCCAATGCGCGGGTGATCACCAGATCGCGGATCGACAATGGTTCTGCAAGCAGTTGTTCGATCGCCTCAAACAGTGGATGTCGCGGGGTTTccccccc > 1:1154775/1‑100 (MQ=255)
cAGCCAATGCGCGGGTGATCACCAGACCGCGGATCGACAATGGTTCTGCAAGCAGTTGTTCGATCGCCTCAAACAGTGGATGTCGCGGGGTTTccccccc > 1:266133/1‑100 (MQ=255)
aGCCAATGCGCGGGTGATCACCAGATCGCGGATCGACAATGGTTCTGCAAGCAGTTGTTCGATCGCCTCAAACAGTGGATGTCGCGGGGTTTCCCCCCCg > 2:1147230/1‑100 (MQ=255)
aGCCAATGCGCGGGTGATCACCAGATCGCGGATCGACAATGGTTCTGCAAGCAGTTGTTCGATCGCCTCAAACAGTGGATGTCGCGGGGTTTCCCCCCCg < 2:1550957/100‑1 (MQ=255)
cAATGCGCGGGTGATCACCAGATCGCGGATCGACAATGGTTCTGCAAGCAGTTGTTCGATCGCCTCAAACAGTGGATGTCGAGGGGTTTCCCCCCCGGcc < 1:179685/100‑1 (MQ=255)
tGCGCGGGTGATCACCAGATCGCGGATCGACAATGGTTCTGCAAGCAGTTGTTCGATCGCCTCAATCAGTGGATGTCGCGGGGTTTCCCCCCCGGCCTTc < 2:222265/100‑1 (MQ=255)
gcgcGGGTGATCACCAGATCGCGGATCGACAATGGTTCTGCAAGCAGTTGTTCGATCGCCTCAAACCGTGGATGTCGCGGGGTTTCCCCCCCGGCCTTCg > 1:391184/1‑100 (MQ=255)
cgcgGGTGATCACCAGATCGCGGATCGACAATGGTTCTGCAAGCAGTTGTTCGATCGCCTCAAACAGTGGATGTCGCGGGGTTTCCCCCCCGGCCTTCGt > 1:180438/1‑100 (MQ=255)
gcgGGTGATCACCAGATCGCGGATCGACAATGGTTCTGCAAGCAGTTGTTCGATCGCCTCAAACAGTGGATGTCGCGGGGTTTCCCCCCCGGCCTTCGTg > 2:482734/1‑100 (MQ=255)
gggTGATCACCAGATCGCGGATCGACAATGGTTCTGCAAGCAGTTGTTCGATCGCCTCAAACAGTGGATGTCGCGGGGTTTCCCCCCCGGCCTTCGTGCg < 2:1372856/100‑1 (MQ=255)
gggTGATCACCAGATCGCGGATCGACAATGGTTCTGCAAGCAGTTGTTCGATCGCCTCAAACAGTGGATGTCGCGGGGTTTCCCCCCCGGCCTTCGTGCg < 1:1145361/100‑1 (MQ=255)
ggTGATCACCAGATCGCGGATCGACAATGGTTCTGCAAGCAGTTGTTCGATCGCCTCAAACAGTGGATGTCGCGGGGTTTCCCCCCCGGCCTTCGTGCGa > 2:1223311/1‑100 (MQ=255)
gTGATCACCAGATCGCGGATCGACAATGGTTCTGCAAGCAGTTGTTCGATCGCCTCAAACAGTGGATGTCGCGGGGTTTCCCCCCCGGCCTTCGTGCGAt > 2:1456177/1‑100 (MQ=255)
cACCAGATCGCGGATCGACAATGGTTCTGCAAGCAGTTGTTCGATCGCCTCAAACAGTGGATGTCGCGGGGTTTCCCCCCCGGCCTTCGTGCGAtcttct > 1:669221/1‑100 (MQ=255)
aCCAGATCGCGGATCGACAATGGTTCTGCAAGCAGTTGTTCGATCGCCTCAAACAGTGGATGTCGCGGGGTTTCCCCCCCGGCCTTCGTGCGATCTTCTa < 1:482730/100‑1 (MQ=255)
aCCAGATCGCGGATCGACAATGGTTCTGCAAGCAGTTGTTCGATCGCCTCAAACAGTGGATGTCGCGGGGTTTCCCCCCCGGCCTTCGTGCGATCTTCTa < 2:409299/100‑1 (MQ=255)
cAGATCGCGGATCGACAATGGTTCTGCAAGCAGTTGTTCGATCGCCTCAAACAGTGGATGTCGCGGGGTTTCCCCCCCGGCCTTCGTGCGATCTTCTAAg < 2:727749/100‑1 (MQ=255)
aTCGCGGATCGACAATGGTTCTGCAAGCAGTTGTTCGATCGCCTCAAACAGTGGATGTCGCGGGGTTTCCCCCCCGGCCTTCGTGCGATCTTCTAAGaaa > 2:49901/1‑100 (MQ=255)
gATCGACAATGGTTCTGCAAGCAGTTGTTCGATCGCCTCAAACAGTGGATGTCGCGGGGTTTCCCCCCCGGCCTTCGTGCGATCTTCTAAGAAACGCTgg > 2:1197410/1‑100 (MQ=255)
aTCGACAATGGTTCTGCAAGCAGTTGTTCGATCGCCTCAAACAGTGGATGTCGCGGGGTTTCCCCCCCGGCCTTCGTGCGATCTTCTAAGAAACGCTggg < 2:1010667/100‑1 (MQ=255)
aTCGACAATGGTTCTGCAAGCAGTTGTTCGATCGCCTCAAACAGTGGATGTCGCGGGGTTTCCCCCCCGGCCTTCGTGCGATCTTCTAAGAAACGCTggg < 1:413439/100‑1 (MQ=255)
cGACAATGGTTCTGCAAGCAGTTGTTCGATCGCCTCAAACAGTGGATGTCGCGGGGTTTCCCCCCCGGCCTTCGTGCGATCTTCTAAGAAACGCTGGgag > 1:1044034/1‑100 (MQ=255)
cGACAATGGTTCTGCAAGCAGTTGTTCGATCGCCTCAAACAGTGGATGTCGCGGGGTTTCCCCCCCGGCCTTCGTGCGATCTTCTAAGAAACGCTGGgag < 1:844387/100‑1 (MQ=255)
cGACAATGGTTCTGCAAGCAGTTGTTCGATCGCCTCAAACAGTGGATGTCGCGGGGTTTCCCCCCCGGCCTTCGTGCGATCTTCTAAGAAACGCTGGgag > 1:1543197/1‑100 (MQ=255)
cGACAATGGTTCTGCAAGCAGTTGTTCGATCGCCTCAAACAGTGGATGTCGCGGGGTTTCCCCCCCGGCCTTCGTGCGATCTTCTAAGAAACGCTGGgag > 1:1238324/1‑100 (MQ=255)
ggTTCTGCAAGCAGTTGTTCGATCGCCTCAAACAGTGGATGTCGCGGGGTTTCCCCCCCGGCCTTCGTGCGATCTTCTAAGAAACGCTGGGAGAAttttt < 2:1425108/100‑1 (MQ=255)
ggTTCTGCAAGCAGTTGTTCGATCGCCTCAAACAGTGGATGTCGCGGGGTTTCCCCCCCGGCCTTCGTGCGATCTTCTAAGAAACGCTGGGAGAAttttt < 2:526644/100‑1 (MQ=255)
gTTCTGCAAGCAGTTGTTCGATCGCCTCAAACAGTGGATGTCGCGGGGTTTCCCCCCCGGCCTTCGTGCGATCTTCTAAGAAACGCTGGGAGAATTTTTc < 2:894690/100‑1 (MQ=255)
ttCTGCAAGCAGTTGCTCGATCGCCTCAAACAGTGGATGTAGCGGGGGTTCCCCCCCGGCCTTTGTGCGATCTTCTAAGAAACGCTGGGAGAATTTTTcc < 1:152867/100‑1 (MQ=255)
tccTGCAAGCAGTTGTTCGATCGCCTCAAACAGTGGATGTCGCGGGGTTTCCCCCCCGGCCTTCGTGCGATCTTCTAAGAAACGCTGGGAGAATTTTTcc > 2:1082578/3‑100 (MQ=255)
gCAAGCAGTTGTTCGATCGCCTCAAACAGTGGATGTCGCGGGGTTTCCCCCCCGGCCTTCGTGCGATCTTCTAAGAAACGCTGGGAGAATTTTTCCAGCg < 2:1303579/100‑1 (MQ=255)
gCAAGCAGTTGTTCGATCGCCTCAAACAGTGGATGTCGCGGGGTTTCCCCCCCGGCCTTCGTGCGATCTTCTAAGAAACGCTGGGAGAATTTTTCCAGCg > 1:996391/1‑100 (MQ=255)
gCAAGCAGTTGTTCGATCGCCTCAAACAGTGGATGTCGCGGGGTTTCCCCCCCGGCCTTCGTGCGATCTTCTAAGAAACGCTGGGAGAATTTTTCCAGCg < 1:1545143/100‑1 (MQ=255)
gCAAGCAGTTGTTCGATCGCCTCAAACAGTGGATGTCGCGGGGTTTCCCCCCCGGCCTTCGTGCGATCTTCTAAGAAACGCTGGGAGAATTTTTCCAGCg < 1:1063010/100‑1 (MQ=255)
cAGTTGTTCGATCGCCTCAAACAGTGGATGTCGCGGGGTTTCCCCCCCGGCCTTCGTGCGATCTTCTAAGAAACGCTGGGAGAATTTTTCCAGCGACTcc < 2:1212974/100‑1 (MQ=255)
aGTTGTTCGATCGCCTCAAACAGTGGATGTCGCGGGGTTTCCCCCCCGGCCTTCGTGCGATCTTCTAAGAAACGCTGGGAGCATTTTTCCCGCGACTCCg > 1:1300197/1‑100 (MQ=255)
aGTTGTTCGATCGCCTCAAACAGTGGATGTCGCGGGGTTTCCCCCCCGGCCTTCGTGCGATCTTCTAAGAAACGCTGGGAGAATTTTTCCAGCGACTCCg > 2:499035/1‑100 (MQ=255)
gttgttCGATCGCCTCAAACAGTGGAGGTCGCGGGGTTTCCCCCCCGCCCTTCGTGCGATCTTCGAAGAAACGCTGGGAGAATTTTTCCAGCGACTCCgg < 1:1448006/100‑1 (MQ=255)
|
CACGGCGGCGTTTTTCACGCGCTACTGTTTCGCGGATCTCAGCCAATGCGCGGGTGATCACCAGATCGCGGATCGACAATGGTTCTGCAAGCAGTTGATCGATCGCCTCAAACAGTGGATGTCGCGGGGTTTCCCCCCCGGCCTTCGTGCGATCTTCTAAGAAACGCTGGGAGAATTTTTCCAGCGACTCCGG > NC_000913/2954965‑2955157
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
