Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A13 F52 I0 R1 129 152.3 7640533 93.0% 7105695 99.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 657,533 A→T 18.3% intergenic (+32/+22) cspE → / ← flc constitutive cold shock family transcription antitermination protein; negative regulator of cspA transcription; RNA melting protein; ssDNA‑binding protein/fluoride efflux channel, dual topology membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913657,5330AT18.3% 334.6 / 68.5 142intergenic (+32/+22)cspE/flcconstitutive cold shock family transcription antitermination protein; negative regulator of cspA transcription; RNA melting protein; ssDNA‑binding protein/fluoride efflux channel, dual topology membrane protein
Reads supporting (aligned to +/- strand):  ref base A (49/67);  new base T (5/21);  total (54/88)
Fisher's exact test for biased strand distribution p-value = 4.28e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.77e-01

Only 100 of 143 total aligned reads displayed.

GCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAAC  >  NC_000913/657435‑657632
                                                                                                  |                                                                                                   
gcgcGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAAt                                                                                                    >  1:2261709/1‑100 (MQ=255)
 ctcgTAGAGTTCTAAATCATTAACAGTGCCAAAGTCCATTCTGCTGCAAACGTAATCGCTCTGTAAAACACGTCAGCAAGAATTCAAAACCCGCTTAATc                                                                                                   <  1:647798/98‑1 (MQ=255)
   cgTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAg                                                                                                 <  1:1790227/100‑1 (MQ=255)
       gagTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCggg                                                                                             <  1:2026934/100‑1 (MQ=255)
       gagTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCggg                                                                                             >  1:1793256/1‑100 (MQ=255)
       gagTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCggg                                                                                             <  1:2648339/100‑1 (MQ=255)
       gagTTCAAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCggg                                                                                             >  2:3505727/1‑100 (MQ=255)
         gTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGtt                                                                                           >  1:3247116/1‑100 (MQ=255)
         gTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGtt                                                                                           >  2:2933175/1‑100 (MQ=255)
         gTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGtt                                                                                           >  2:2132664/1‑100 (MQ=255)
         gTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGtt                                                                                           >  2:549176/1‑100 (MQ=255)
         gTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGt                                                                                            >  1:1275216/1‑99 (MQ=255)
         gTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCCCTGTAAGCTACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCggg                                                                                             >  1:460098/1‑98 (MQ=255)
          ttCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTGATTAAGCGGGttt                                                                                          <  1:2909926/100‑1 (MQ=255)
           tCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTGATTAAGCGGGtttt                                                                                         <  1:3811660/100‑1 (MQ=255)
           tCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTGATTAAGCGGGtttt                                                                                         <  2:3265742/100‑1 (MQ=255)
           tCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTGATTAAGCGGGtttt                                                                                         <  2:1059452/100‑1 (MQ=255)
            cGAAATCACTAACGGTGCCAAAGGTCCTTCTGCTCCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTGATTAAGCGGGttttg                                                                                        <  2:2632871/100‑2 (MQ=38)
            cGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGttttt                                                                                        >  2:3715936/1‑100 (MQ=255)
            cGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTGATTAAGCGGGttttg                                                                                        <  1:1621246/100‑2 (MQ=39)
             gAAATCACTAACGGTGCCAAAGGCCCTTCTGCTTCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTGATTAAGCGGGttttga                                                                                       <  1:87426/100‑3 (MQ=38)
             gAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGtttttt                                                                                       >  1:3699965/1‑100 (MQ=255)
             gAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTGATTAAGCGGGttttga                                                                                       <  2:2714487/100‑3 (MQ=38)
              aaaaCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACATCAGCAAGAATTCAAAACCCGCTGATTAAGCGGGttttgaa                                                                                      <  1:1199033/100‑4 (MQ=38)
              aaaTCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTGATTAAGCGGGttttgaa                                                                                      <  2:2250295/100‑4 (MQ=38)
              aaaTCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTGATTAAGCGGGttttgaa                                                                                      <  2:665183/100‑4 (MQ=39)
               aaTCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGtttttttt                                                                                     <  1:3003211/100‑1 (MQ=255)
               aaTCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTGATTAAGCGGGttttgaat                                                                                     <  2:2195036/100‑5 (MQ=38)
               aaTCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTGATTAAGCGGGttttgaat                                                                                     <  2:2190544/100‑5 (MQ=38)
               aaTCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTGATTAAGCGGGttttgaat                                                                                     <  1:3423952/100‑5 (MQ=38)
               aaTCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTGATTAAGCGGGttttgaat                                                                                     <  1:1537282/100‑5 (MQ=38)
                aTCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTg                                                                                    <  1:2867589/100‑1 (MQ=255)
                 tCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTGAATCAGCGGGTTTTTTTTgg                                                                                   <  1:1050419/100‑1 (MQ=255)
                  cACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGt                                                                                  >  2:1897550/1‑100 (MQ=255)
                  cACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGt                                                                                  >  1:271663/1‑100 (MQ=255)
                    cTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCt                                                                                >  1:1008449/1‑100 (MQ=255)
                      aaCGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCttt                                                                              <  1:3267193/100‑1 (MQ=255)
                      aaCGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCttt                                                                              <  2:1398764/100‑1 (MQ=255)
                       aCGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTa                                                                             <  1:405539/100‑1 (MQ=255)
                          gTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAgtg                                                                          <  1:486860/100‑1 (MQ=255)
                            gCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGtgtg                                                                        <  2:2093273/100‑1 (MQ=255)
                            gCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGtgtg                                                                        >  2:3599707/1‑100 (MQ=255)
                            gCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGtgtg                                                                        >  2:866190/1‑100 (MQ=255)
                             ccAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGc                                                                       <  2:719578/100‑1 (MQ=255)
                              cAAAGGCCCTTCTGATGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGtttttttttttttt                                                                               >  1:2247349/1‑85 (MQ=255)
                               aaaGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCgg                                                                     <  1:810635/100‑1 (MQ=255)
                                aaGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGt                                                                    <  1:2885512/100‑1 (MQ=255)
                                  ggCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTg                                                                  <  2:398391/100‑1 (MQ=255)
                                   gCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGa                                                                 <  2:2476063/100‑1 (MQ=255)
                                    cccTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAg                                                                <  1:836563/100‑1 (MQ=255)
                                    cccTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAg                                                                <  2:623006/100‑1 (MQ=255)
                                    cccTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAg                                                                >  1:923040/1‑100 (MQ=255)
                                     ccTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAgg                                                               <  1:1296363/100‑1 (MQ=255)
                                     ccTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAgg                                                               <  1:1296404/100‑1 (MQ=255)
                                       ttCTGCTGCAAACGTAACCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGcc                                                             >  1:731126/1‑100 (MQ=255)
                                           gctgcAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGaaa                                                         <  1:561699/100‑1 (MQ=255)
                                           gctgcAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGaaa                                                         >  1:3588324/1‑100 (MQ=255)
                                           gctgcAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCAGGTTTTTTTTGGTCTTTAGTGTGCGGATGAGGTCGaaa                                                         >  1:3227507/1‑100 (MQ=255)
                                             tgcAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAAc                                                       >  2:938896/1‑100 (MQ=255)
                                             tgcAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAAc                                                       >  2:3080887/1‑100 (MQ=255)
                                              gcAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACa                                                      <  2:3365818/100‑1 (MQ=255)
                                              gcAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACa                                                      <  1:1897541/100‑1 (MQ=255)
                                               cAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAg                                                     <  2:3809176/100‑1 (MQ=255)
                                                aaaCGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACACGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGc                                                    >  1:2440114/1‑100 (MQ=255)
                                                 aaCGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGcc                                                   <  2:3458742/100‑1 (MQ=255)
                                                 aaCGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGcc                                                   <  1:468011/100‑1 (MQ=255)
                                                    gTAATCGCTGTGTAAGATACGTCAGCAAGAAGGGAAAGCCCGCTTGATCAGCGGGTTTTGTTTGGGCTTTAGTGTGCGGTTGAGGCCGGAAACAGCCAg                                                 <  1:888232/99‑1 (MQ=255)
                                                         cGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGcc                                           <  1:3818462/100‑1 (MQ=255)
                                                           ctctGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTGTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAg                                         <  1:1859508/100‑1 (MQ=255)
                                                              tGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGc                                      <  2:2491991/100‑1 (MQ=255)
                                                              tGTAAGATACGTCAGCAAGAATTAAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTAAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGc                                      >  2:2685182/1‑100 (MQ=255)
                                                               gTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCg                                     >  2:2424131/1‑100 (MQ=255)
                                                                tAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCgg                                    <  1:74562/100‑1 (MQ=255)
                                                                 aaGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGt                                   <  2:1467808/100‑1 (MQ=255)
                                                                  aGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTc                                  <  2:549342/100‑1 (MQ=255)
                                                                       cGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGc                             <  2:13877/100‑1 (MQ=255)
                                                                       cGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGc                             >  2:1525833/1‑100 (MQ=255)
                                                                         tCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCaa                           >  2:1357887/1‑100 (MQ=255)
                                                                          cAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCaaa                          <  1:1357879/100‑1 (MQ=255)
                                                                           aGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGTTCTTTAGTGTGCGGTTGAAGCCGAAAACAGCCAGCATGGCAGTGCGGTCATGGCacaa                         >  1:1406659/1‑100 (MQ=255)
                                                                           aGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGTCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCaaaa                         >  2:452077/1‑100 (MQ=255)
                                                                            gCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAg                        <  2:1051219/100‑1 (MQ=255)
                                                                            gCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAg                        <  2:364616/100‑1 (MQ=255)
                                                                            gCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAg                        >  2:1641018/1‑100 (MQ=255)
                                                                             cAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCaagatc                       >  2:608447/1‑96 (MQ=255)
                                                                               aGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGAcc                     >  2:934993/1‑100 (MQ=255)
                                                                                gAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGAccc                    <  2:1498635/100‑1 (MQ=255)
                                                                                 aaTTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGAcccc                   <  1:1027332/100‑1 (MQ=255)
                                                                                  aaaaaaaaCCCGCTGATTAAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCa                  >  2:3543053/5‑100 (MQ=38)
                                                                                  aTTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCa                  <  2:3543233/100‑1 (MQ=255)
                                                                                  aTTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCa                  >  2:1572823/1‑100 (MQ=255)
                                                                                  aTTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCa                  <  2:3588346/100‑1 (MQ=255)
                                                                                   aaaaaaaCCCGCTGATTAAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAg                 >  2:589705/4‑100 (MQ=38)
                                                                                    aaaaaaCCCGCTGATTAAGCGGGTTTTTTTTGGTCTTTAGTGTTCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACACCAGa                >  1:3042732/3‑100 (MQ=38)
                                                                                      aaaaCCCGCTGATTAAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAg              >  2:3275517/1‑100 (MQ=255)
                                                                                         aCCCGCTGATTAAGCGGGTTTTTTTTGGTTTTTAGTGCTCGGTTGAGGCCGAAAACAGCAAGAATGTCAGTGCGGTCATGGCCCAAGACCCCAGaaag             >  1:408990/1‑96 (MQ=255)
                                                                                          cccGCTTAATCAGCGGGTTTTTTTTTTTTTTTTTTTTTCTGTTGAGGCCGAAATCAGCCAGAATGCCATTGCGGTCATGGCAAAAGACCCCAGAAGGtt           >  1:2720117/1‑99 (MQ=255)
                                                                                           ccGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGa         <  2:68153/100‑1 (MQ=255)
                                                                                            cGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGAc        <  1:1463186/100‑1 (MQ=255)
                                                                                                  aTCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTAATGGCAAAAGACCCCAGAAGGTTGACGAAAAc  >  2:2382558/1‑100 (MQ=255)
                                                                                                  |                                                                                                   
GCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAAC  >  NC_000913/657435‑657632

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A