Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F113 I0 R1 46 122.5 6222771 91.8% 5712503 99.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 657,531 T→G 15.3% intergenic (+30/+24) cspE → / ← flc constitutive cold shock family transcription antitermination protein; negative regulator of cspA transcription; RNA melting protein; ssDNA‑binding protein/fluoride efflux channel, dual topology membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913657,5310TG15.3% 268.0 / 38.1 102intergenic (+30/+24)cspE/flcconstitutive cold shock family transcription antitermination protein; negative regulator of cspA transcription; RNA melting protein; ssDNA‑binding protein/fluoride efflux channel, dual topology membrane protein
Reads supporting (aligned to +/- strand):  ref base T (45/41);  new base G (7/9);  total (52/50)
Fisher's exact test for biased strand distribution p-value = 5.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 104 total aligned reads displayed.

TCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACG  >  NC_000913/657432‑657627
                                                                                                   |                                                                                                
tCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTGTGTAAGATACGTCAGCAAGAATTCAAAACCCGCtt                                                                                                  <  1:1267836/100‑1 (MQ=255)
tCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCtt                                                                                                  <  2:2975739/100‑1 (MQ=255)
 cAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTa                                                                                                 >  2:1647109/1‑100 (MQ=255)
  aGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTCAGATACGTCAGCAAGACTTCACAACCCGCCTaa                                                                                                >  2:764467/1‑100 (MQ=255)
    cgcgTAGAGTTCGAAATCACTAACGGTGCCAATGGCCCTCCTACTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATc                                                                                              <  2:2184135/100‑1 (MQ=255)
        tatagTTCGAAATCACGAACGATAACAAAGGCCCTTCTGCTGCAAGCGTAATCGCACTGTAAGATACGTCAGCAAGAATTCAAAACTCGCTTAATCAGCg                                                                                          <  2:1280888/97‑1 (MQ=255)
        tAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGcn                                                                                          <  1:484149/100‑2 (MQ=255)
        tAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCg                                                                                          >  2:343448/1‑100 (MQ=255)
        tAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCg                                                                                          <  2:1785357/100‑1 (MQ=255)
        tAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCg                                                                                          <  1:1326942/100‑1 (MQ=255)
        ctgagTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCg                                                                                          <  1:737115/98‑1 (MQ=255)
          gagTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCggg                                                                                        <  2:1804242/100‑1 (MQ=255)
          gagTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCgag                                                                                        >  2:737113/1‑98 (MQ=255)
           agTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGt                                                                                       <  1:892013/100‑1 (MQ=255)
              tCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTGATTAAGCGGGtttt                                                                                    <  1:1059483/100‑1 (MQ=255)
              tCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTGATTAAGCGGGtttt                                                                                    <  2:314348/100‑1 (MQ=255)
              tCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTGATTAAGCGGGtttt                                                                                    <  1:2460355/100‑1 (MQ=255)
               cGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTGATTAAGCGGGttttg                                                                                   <  1:586382/100‑2 (MQ=39)
               cGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTGATTAAGCGGGttttg                                                                                   <  2:1213732/100‑2 (MQ=39)
               cGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTGATTAAGCGGGttttg                                                                                   <  2:2813248/100‑2 (MQ=39)
               cGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTGATTAAGCGGGttttg                                                                                   <  2:463011/100‑2 (MQ=39)
                gAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGtttttt                                                                                  >  1:2388622/1‑100 (MQ=255)
                gAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTGATTAAGCGGGttttga                                                                                  <  1:83483/100‑3 (MQ=38)
                   aTCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGtttttttt                                                                                >  2:2244068/1‑99 (MQ=255)
                   aTCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTg                                                                               <  1:2274598/100‑1 (MQ=255)
                         aaCGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCttt                                                                         >  1:1430373/1‑100 (MQ=255)
                           cGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAg                                                                       <  2:2914670/100‑1 (MQ=255)
                           cGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAg                                                                       <  2:586936/100‑1 (MQ=255)
                             gTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAgtg                                                                     <  1:2781333/100‑1 (MQ=255)
                               gCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGtgtg                                                                   >  1:1206796/1‑100 (MQ=255)
                                ccAACGGCCCTTGTGAAGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGc                                                                  <  1:1231674/100‑1 (MQ=255)
                                ccAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGc                                                                  >  2:1290802/1‑100 (MQ=255)
                                 cAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCg                                                                 >  2:2140505/1‑100 (MQ=255)
                                  aaaGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCgg                                                                <  1:2833720/100‑1 (MQ=255)
                                    aGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGtt                                                              >  2:2170260/1‑100 (MQ=255)
                                       cccTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAg                                                           >  1:71518/1‑100 (MQ=255)
                                        ccTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAgg                                                          <  1:2910322/100‑1 (MQ=255)
                                         cTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAg                                                           >  1:2392239/1‑98 (MQ=255)
                                           tCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCg                                                       <  2:1354086/100‑1 (MQ=255)
                                              gctgcAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGaaa                                                    >  1:1304681/1‑100 (MQ=255)
                                              gctgcAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGaaa                                                    <  2:1150525/100‑1 (MQ=255)
                                               ctgcAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAAGCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGaaaa                                                   >  1:3111088/1‑100 (MQ=255)
                                               ctgcAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGaaaa                                                   >  1:557629/1‑100 (MQ=255)
                                                tgcAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAAc                                                  <  2:2457583/100‑1 (MQ=255)
                                                  cAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAg                                                >  1:2318943/1‑100 (MQ=255)
                                                  cAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAg                                                >  1:584053/1‑100 (MQ=255)
                                                  cAAACGGAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAg                                                >  1:1341352/1‑100 (MQ=255)
                                                   aaaCGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGc                                               <  1:2515864/100‑1 (MQ=255)
                                                    aaCGTAATCGCTCTGTAAGATACGTCAGCAAGAAGTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGcc                                              <  2:717076/100‑1 (MQ=255)
                                                     aCGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCa                                             <  1:83181/100‑1 (MQ=255)
                                                      cGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAg                                            <  2:1681768/100‑1 (MQ=255)
                                                      cGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAg                                            >  2:60802/1‑100 (MQ=255)
                                                         aaTCGCTCTGTAAGATACGTCAGCAAGAGTTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAAt                                         >  1:1250942/1‑100 (MQ=255)
                                                          aTCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATg                                        >  2:2522237/1‑100 (MQ=255)
                                                            cGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGcc                                      >  2:71053/1‑100 (MQ=255)
                                                            cGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGcc                                      <  2:1407833/100‑1 (MQ=255)
                                                                  ntAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCg                                >  1:2591857/2‑100 (MQ=255)
                                                                  gTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCg                                >  2:2915460/1‑100 (MQ=255)
                                                                   tAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCgg                               >  2:1198793/1‑100 (MQ=255)
                                                                    aaGATACGTCAGCAAGAATTCAAACCCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCgg                               <  1:1855580/99‑1 (MQ=255)
                                                                     aGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTc                             >  1:1293439/1‑100 (MQ=255)
                                                                      gATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCa                            <  1:2269673/100‑1 (MQ=255)
                                                                      gATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCa                            <  1:2170279/100‑1 (MQ=255)
                                                                          cGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGGGGATGAGTCAGAAAACAGCCAGAATGCCAGTGCGGTCATGGc                        >  2:2183038/1‑100 (MQ=255)
                                                                          cGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGc                        <  2:1007443/100‑1 (MQ=255)
                                                                          cGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTGTGTTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGc                        <  1:1033096/100‑1 (MQ=255)
                                                                           gTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCa                       <  1:1114318/100‑1 (MQ=255)
                                                                           gTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCa                       <  1:736174/100‑1 (MQ=255)
                                                                            tCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCaa                      <  1:2404045/100‑1 (MQ=255)
                                                                             cggCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCaaa                     >  1:772504/3‑100 (MQ=255)
                                                                             cAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGTAAACAGCCAGAATGCCAGTGCGGTCATGGCaaa                     <  2:2025898/100‑1 (MQ=255)
                                                                             cAGCAAGAATTCAAAACCCGCTTAATCAGAGGGATTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAAAAGACAGAATGCCAGTGCGGTCATGGCaaa                     >  2:212348/1‑100 (MQ=255)
                                                                               gCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAg                   >  1:894842/1‑100 (MQ=255)
                                                                                cAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGa                  >  1:2384999/1‑100 (MQ=255)
                                                                                 aaGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGAc                 >  1:2424648/1‑100 (MQ=255)
                                                                                  accaaaaaaaaCCCGCTGATTAAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGAcc                >  1:1101510/8‑100 (MQ=37)
                                                                                  accaaaaaaaaCCCGCTGATTAAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGAcc                >  1:2494074/8‑100 (MQ=37)
                                                                                  aGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTCGTATGCGGATGCAGCCGAAAACAGCCAAAATGCCAGTGCGGTAATGGCcaa                     >  1:256994/1‑95 (MQ=255)
                                                                                  aGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGAcc                <  1:1290811/100‑1 (MQ=255)
                                                                                  aGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGAcc                >  1:2065861/1‑100 (MQ=255)
                                                                                   ccaaaaaaaaCCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGAccc               >  1:1460965/7‑100 (MQ=255)
                                                                                   ccaaaaaaaaCCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGAccc               >  1:14655/7‑100 (MQ=255)
                                                                                    caaaaaaaaCCCGCTTAATCAGCGGGTTTTTCTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGAcccc              <  2:2090783/95‑1 (MQ=255)
                                                                                    aaTTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGAcccc              <  2:1293430/100‑1 (MQ=255)
                                                                                    aaTTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGAcccc              >  2:2497238/1‑100 (MQ=255)
                                                                                    aaTTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGAcccc              <  1:2522262/100‑1 (MQ=255)
                                                                                     aaaaaaaaCCCGCTGATTAAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCTAGTGCGGTCATGGCAAAAGACCCCa             >  2:165039/5‑100 (MQ=38)
                                                                                     aaaaaaaaCCCGCTGATTAAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCa             >  1:1940302/5‑100 (MQ=38)
                                                                                     aaaaaaaaCCCGCTGATTAAGCGGGTTTTTTTTGGTCTTAAGTGTGCGGTTGAGGCCGAAAATAGCGAGAATGCCAGTGCGGTCATGGCAAAAGACACCa             >  2:2219809/5‑100 (MQ=25)
                                                                                     aTTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCa             >  1:2547170/1‑100 (MQ=255)
                                                                                      ttCAAAACCCGCTTAATCAGCGGGTTTTTTTTTTTTTTTTTTGTGCGGTTGAGGCCGAAAACAGCCTGAATTCCCTTGCGGTCATGGCAAAAGACTCCAg            >  2:2884896/1‑100 (MQ=255)
                                                                                       tCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTTTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGa           >  1:478068/1‑100 (MQ=255)
                                                                                       tCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGa           >  1:487885/1‑100 (MQ=255)
                                                                                         aaaaCCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAg         <  1:2455677/100‑1 (MQ=255)
                                                                                         aaaaCCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAg         <  1:2366367/100‑1 (MQ=255)
                                                                                         aaaaCCCGCTGATTAAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAg         >  1:637246/1‑100 (MQ=255)
                                                                                           aaCCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGt       <  1:2913130/100‑1 (MQ=255)
                                                                                            aCCCGCTTAATCAGCGGGTTTTTTTTGGTTTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGtt      >  2:1778879/1‑100 (MQ=255)
                                                                                                gCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACg  <  1:524342/100‑1 (MQ=255)
                                                                                                gCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACg  >  2:1731927/1‑100 (MQ=255)
                                                                                                   |                                                                                                
TCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACG  >  NC_000913/657432‑657627

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A