Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A16 F125 I0 R1 327 181.4 8809959 94.1% 8290171 99.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 657,531 T→G 20.7% intergenic (+30/+24) cspE → / ← flc constitutive cold shock family transcription antitermination protein; negative regulator of cspA transcription; RNA melting protein; ssDNA‑binding protein/fluoride efflux channel, dual topology membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913657,5310TG20.7% 375.9 / 106.4 174intergenic (+30/+24)cspE/flcconstitutive cold shock family transcription antitermination protein; negative regulator of cspA transcription; RNA melting protein; ssDNA‑binding protein/fluoride efflux channel, dual topology membrane protein
Reads supporting (aligned to +/- strand):  ref base T (61/77);  new base G (16/20);  total (77/97)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.61e-01

Only 100 of 176 total aligned reads displayed.

TCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGA  >  NC_000913/657432‑657628
                                                                                                   |                                                                                                 
tCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCtt                                                                                                   <  1:2858467/100‑1 (MQ=255)
 cAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTa                                                                                                  <  1:2919545/100‑1 (MQ=255)
  aGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTaa                                                                                                 <  1:1524667/100‑1 (MQ=255)
  aGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTaa                                                                                                 <  2:1843091/100‑1 (MQ=255)
  aGCGCGTAGAGTTCGAAAGCACTAACGGTGCCAAAGCCCCTTCTGCTGCAAACGTAATCGCGCTGTAAGATACGTCAGCAAGAATTTAAAACCCGCTTaa                                                                                                 <  1:2721932/100‑1 (MQ=255)
   gcgcGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAAt                                                                                                >  2:3396089/1‑100 (MQ=255)
    cgcgTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATc                                                                                               <  2:2094297/100‑1 (MQ=255)
      cgTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAg                                                                                             >  2:3500261/1‑100 (MQ=255)
       gTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGc                                                                                            <  1:3500243/100‑1 (MQ=255)
        tAGAGTTCGAAATCACTAACGGTGCCAAAGGCTCGTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCTCTTCATCAGCg                                                                                           <  1:2310058/100‑1 (MQ=255)
        tAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCg                                                                                           <  2:2897831/100‑1 (MQ=255)
        tAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCg                                                                                           <  1:1991323/100‑1 (MQ=255)
           agTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTGATTAAGCGGGt                                                                                        <  1:847327/100‑1 (MQ=255)
            gTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTGATTAAGCGGGtt                                                                                       <  2:1651494/100‑1 (MQ=255)
              tCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGtttt                                                                                     <  2:625226/100‑1 (MQ=255)
              tCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTGATTAAGCGGGtttt                                                                                     <  2:3509379/100‑1 (MQ=255)
               cGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTGATTAAGCGGGttttg                                                                                    <  1:1248029/100‑2 (MQ=39)
               cGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTGATTAAGCGGGttttg                                                                                    <  1:1594849/100‑2 (MQ=39)
               cGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTGATTAAGCGGGttttg                                                                                    <  1:598775/100‑2 (MQ=39)
                gAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGtttttt                                                                                   >  1:335850/1‑100 (MQ=255)
                gAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTGATTAAGCGGGttttga                                                                                   <  1:3877124/100‑3 (MQ=38)
                gAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTGATTAAGCGGGttttga                                                                                   <  1:3559352/100‑3 (MQ=38)
                  aaTCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGtttttttt                                                                                 <  2:3498445/100‑1 (MQ=255)
                  aaTCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTGATTAAGCGGGttttgaat                                                                                 <  1:4398553/100‑5 (MQ=38)
                  aaTCACTAACGGTGCCAAAAGCCCTTCTTCTGTAAACGTAATCGATCTGTAAGATACGTCAGCAAGAATTCAACCCCCGCTGATTAAGCGGGttttgaat                                                                                 <  2:3615597/100‑5 (MQ=255)
                   aTCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTg                                                                                <  2:1240814/100‑1 (MQ=255)
                   aTCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTg                                                                                <  2:2726843/100‑1 (MQ=255)
                   aTCACTAACGGTCGCATAGGCCCTTCAGCTGCAAACGCAATCGTTCTGTAATATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTg                                                                                <  1:3908054/100‑1 (MQ=255)
                    tCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTCTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTg                                                                                <  2:1495169/99‑1 (MQ=255)
                       cTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCACGAATTCAAAACCCGCTTAATCAGCGGGtttttttttttct                                                                            >  1:2773909/1‑95 (MQ=255)
                       cTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCt                                                                            >  2:2405190/1‑100 (MQ=255)
                       cTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCt                                                                            <  2:2139648/100‑1 (MQ=255)
                           cGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAg                                                                        <  1:1051985/100‑1 (MQ=255)
                             gTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAgtg                                                                      <  2:4107344/100‑1 (MQ=255)
                                    aGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGtt                                                               <  1:1770268/100‑1 (MQ=255)
                                       cccTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAg                                                            <  1:3172016/100‑1 (MQ=255)
                                       cccTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAg                                                            <  2:3069598/100‑1 (MQ=255)
                                         cTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGc                                                          <  2:1859350/100‑1 (MQ=255)
                                          ttCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGcc                                                         <  1:1424236/100‑1 (MQ=255)
                                          ttCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGcc                                                         >  2:3139182/1‑100 (MQ=255)
                                           tCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCg                                                        <  2:4275492/100‑1 (MQ=255)
                                            ctgctgCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGa                                                       <  1:306457/100‑1 (MQ=255)
                                              gctgcAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGaaa                                                     <  1:2905392/100‑1 (MQ=255)
                                              gctgcAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGATTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGaaa                                                     >  2:525305/1‑100 (MQ=255)
                                               ctgcAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGaaaa                                                    >  2:1425103/1‑100 (MQ=255)
                                                tgcAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAAc                                                   >  2:1884214/1‑100 (MQ=255)
                                                tgcAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAAc                                                   >  2:1884902/1‑100 (MQ=255)
                                                tgcAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAAc                                                   >  2:1777741/1‑100 (MQ=255)
                                                tgcAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAAc                                                   <  1:2226593/100‑1 (MQ=255)
                                                  cAAACGTAATCGCTCTGTAAGATACGTCATCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAg                                                 <  1:1338888/100‑1 (MQ=255)
                                                  cAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAg                                                 <  2:242241/100‑1 (MQ=255)
                                                    aaCGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCCAAAACAGcc                                               <  2:3689408/100‑1 (MQ=255)
                                                        tAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGaa                                           >  2:585126/1‑100 (MQ=255)
                                                            cGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGcc                                       >  2:4316301/1‑100 (MQ=255)
                                                            cGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGcc                                       >  2:736865/1‑100 (MQ=255)
                                                             gCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAACACAGCCAGAATGCCa                                      >  1:259805/1‑100 (MQ=255)
                                                             gCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCa                                      >  1:351349/1‑100 (MQ=255)
                                                               tctGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGt                                    <  2:375261/100‑1 (MQ=255)
                                                                ctGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTTTTCTTTAGTTTGCGTTTTAGGCCGAAAACAGCCATAATGCCa                                      >  1:3168310/1‑97 (MQ=255)
                                                                ctGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTg                                   >  1:2460130/1‑100 (MQ=255)
                                                                ctGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTg                                   <  2:4274815/100‑1 (MQ=255)
                                                                ctGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTg                                   >  2:27011/1‑100 (MQ=255)
                                                                  gTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTTCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGc                                  >  1:2098166/1‑99 (MQ=255)
                                                                  gTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCg                                 <  2:3325796/100‑1 (MQ=255)
                                                                   tAAGATACGTCAGCAAGAATTCAAAACCCGATTAATCAGCGGGGTTTTTTTGGTATTTAATGTGCGGTTGAAGACGAAAACAGCCAGAATGCCAATGCg                                 >  1:1602038/1‑99 (MQ=255)
                                                                        tACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATg                           <  1:3139165/100‑1 (MQ=255)
                                                                        tACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATg                           <  1:913664/100‑1 (MQ=255)
                                                                         aCGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATgg                          <  2:328369/100‑1 (MQ=255)
                                                                         aCGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATgg                          <  1:3287767/100‑1 (MQ=255)
                                                                          cGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGc                         >  2:2532370/1‑100 (MQ=255)
                                                                          cGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGc                         <  2:739959/100‑1 (MQ=255)
                                                                           gTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCa                        >  2:786228/1‑100 (MQ=255)
                                                                            tCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTTTTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGTGGTTATggt                         >  1:2585665/1‑97 (MQ=255)
                                                                            tCAGCAAGAATTCAAAACATGCTTAATCAGCGGGATTTTTCTGGTCTTTAGTGTGCGGTTGAGACAGAACACAGTCAGAGTGCCAGTGTGGTGATGGcga                       >  1:4367235/1‑98 (MQ=255)
                                                                              aGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCaaaa                     <  2:495651/100‑1 (MQ=255)
                                                                              aGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCaaaa                     >  2:3239210/1‑100 (MQ=255)
                                                                              aGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGACGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCa                        >  1:1201029/1‑97 (MQ=255)
                                                                                cAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGACTGCCAGTGCGGTCATGGCAAAAGa                   <  2:198866/100‑1 (MQ=255)
                                                                                agaccaaaaaaaaCCCGCTGTTTAAGCGTATTTTTTTTGGCCTTTACAGTGCGGATGAGGCCGGAAACCGCCAGAATGTCAGTGCGGTCATGGCAAAAGa                   >  1:1459143/10‑100 (MQ=255)
                                                                                  accaaaaaaaaCCCGCTGATTAAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGAcc                 >  2:4205732/8‑100 (MQ=37)
                                                                                   gAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGAccc                <  1:1413396/100‑1 (MQ=255)
                                                                                   ccaaaaaaaaCCCGCTGATTAAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGAccc                >  2:2265114/7‑100 (MQ=37)
                                                                                   ccaaaaaaaaCCCGCTGATTAAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTAATGGCAAAAGAacc                >  1:236624/7‑100 (MQ=37)
                                                                                   ccaaaaaaaaCCCGCTGATTAAGCGGGTTTTTTTTGGTCTTTAGTGTGCGCTTGAGGCCTTAAACAGCCAGAATACCACTGCGGTCATGGCAAAAGcccc                >  1:1807411/7‑100 (MQ=21)
                                                                                     aTTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCa              >  2:2121322/1‑100 (MQ=255)
                                                                                     aTTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCa              >  1:4319671/1‑100 (MQ=255)
                                                                                      ttCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAg             >  2:1407980/1‑100 (MQ=255)
                                                                                      ttCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAg             >  2:2490063/1‑100 (MQ=255)
                                                                                      aaaaaaaCCCGCTGATTAAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAg             >  2:1345114/4‑100 (MQ=38)
                                                                                        cAAACCCCGCTTAATCAGCGGGTTTGTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGa            <  1:3770139/99‑1 (MQ=255)
                                                                                         aaaaCCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAg          <  2:706765/100‑1 (MQ=255)
                                                                                         aaaaCCCGCTGATTAAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAg          >  2:1793952/1‑100 (MQ=255)
                                                                                           aaCCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGt        <  2:1918194/100‑1 (MQ=255)
                                                                                            aCCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGtt       >  2:4299471/1‑100 (MQ=255)
                                                                                            aCCCGCTGATTAAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGtt       >  1:1923767/1‑100 (MQ=255)
                                                                                             cccGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTg      >  2:102597/1‑100 (MQ=255)
                                                                                             cccGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTg      <  1:2420996/100‑1 (MQ=255)
                                                                                              ccGCTTAAGCAGCGGGTGTTTTTTGGTCTTTATTGTGCGGTTGAGGCCGAAAAAAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAATGt        <  1:511224/97‑1 (MQ=255)
                                                                                                gCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACg   <  1:3965523/100‑1 (MQ=255)
                                                                                                gCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACg   <  2:886264/100‑1 (MQ=255)
                                                                                                   |                                                                                                 
TCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGA  >  NC_000913/657432‑657628

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A