Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A15 F55 I0 R1
|
245 |
145.6 |
7293262 |
93.0% |
6782733 |
99.8 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
NC_000913 |
3,427,065:1 |
+A |
100% |
intergenic (‑32/+11) |
alaU ← / ← ileU |
tRNA‑Ala/tRNA‑Ile |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,427,065 | 1 | . | A | 50.0%
| ‑6.4
/ 2.8
| 4 | intergenic (‑32/+11) | alaU/ileU | tRNA‑Ala/tRNA‑Ile |
| Reads supporting (aligned to +/- strand): ref base . (0/2); new base A (0/2); total (0/4) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CGAACCGCAGACCTCCTGCGTGCAAAGCAGGCGCTCTCCCAGCTGAGCTATAGCCCCATAACATGTAGTTAAAACCTCTTCAAATTTGC‑CGTGCAAATTTGGTAGGCCTGAGTGGACTTGAACCACCGACCTCACCCTTATCAGGGGTGCGCTCTAACCACCTGA > NC_000913/3426977‑3427141
|
cGAACCGCAGACCTCCTGCGTGCAAAGCAGGCGCTCTCCCAGCTGAGCTATAGCCCCATAACATGTAGTTAAAACCTCTTCAAATTTGCACG‑GCAAAttt < 1:2438495/100‑1 (MQ=1)
cGAACCGCAGACCTCCTGCGTGCAAAGCAGGCGCTCTCCCAGCTGAGCTATAGCCCCATAACATGTAGTTAAAACCTCTTCAAATTTGCACG‑GCAAAttt < 2:2591058/100‑1 (MQ=1)
tgtaaaGCAGGCTCTCTCCCAGGTGAGCTATAGCCTCATTACATGTAGTTAGAACCTCTTCAAATTTGC‑CGTGCACCTTTGGTAGGCCTGAGTGGACTTg < 2:3214565/97‑1 (MQ=1)
gtgaaaaCCTCTTCAAATTTGC‑CGTGCAAATTTGGTAGGCCTGAGTGGACTTGAACCACCGACCTCACCCTTAGCAGGGGTGCGCTCTAACCACCTGa < 2:3019782/95‑1 (MQ=0)
|
CGAACCGCAGACCTCCTGCGTGCAAAGCAGGCGCTCTCCCAGCTGAGCTATAGCCCCATAACATGTAGTTAAAACCTCTTCAAATTTGC‑CGTGCAAATTTGGTAGGCCTGAGTGGACTTGAACCACCGACCTCACCCTTATCAGGGGTGCGCTCTAACCACCTGA > NC_000913/3426977‑3427141
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
