Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F70 I0 R1 14 86.3 4154692 96.9% 4025896 99.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,187,550 C→T 100% A734V (GCG→GTG)  rpoC → RNA polymerase, beta prime subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,187,5500CT95.6% 280.0 / 1.3 88A734V (GCG→GTG) rpoCRNA polymerase, beta prime subunit
Reads supporting (aligned to +/- strand):  ref base C (3/1);  new base T (44/39);  total (48/40)
Fisher's exact test for biased strand distribution p-value = 6.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAG  >  NC_000913/4187456‑4187645
                                                                                              |                                                                                               
aCTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGTGGcac                                                                                            <  2:819769/100‑1 (MQ=255)
 cTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGTGGcaca                                                                                           >  2:457162/1‑100 (MQ=255)
 cTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACAGGATGGCCGACTTCGGTGCGCGTGGTTCTGTGGcaca                                                                                           >  2:78807/1‑100 (MQ=255)
    aaaCCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGTGGCACAGAt                                                                                        >  1:1943875/1‑100 (MQ=255)
    aaaCCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGTGGCACAGAt                                                                                        <  1:1387305/100‑1 (MQ=255)
     aaCCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGTGGCACAGAtt                                                                                       <  2:2055183/100‑1 (MQ=255)
     aaCCGTGATGAACCGTGACGGTCAGGAAGAGAAGCAAGTTTCCATGAACAGCATCTACATGATGGCCGACTCCGGGGCGCGTGGTTCTGTGGCACAGa                                                                                         >  2:1833173/1‑98 (MQ=255)
      aCCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGTGGCACAGATTc                                                                                      >  1:1081405/1‑100 (MQ=255)
       ccGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGTGGCACAGATTCg                                                                                     >  2:609899/1‑100 (MQ=255)
        cGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGTGGCACAGATTCGt                                                                                    >  2:2071846/1‑100 (MQ=255)
         gTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGTGGCACAGATTCGTc                                                                                   <  1:1900159/100‑1 (MQ=255)
         atGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGTGGCACAGATTCGTc                                                                                   <  1:712317/99‑1 (MQ=255)
          tGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGTGGCACAGATTCGTCa                                                                                  >  1:1154153/1‑100 (MQ=255)
          tGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGTGGCACAGATTCGTCa                                                                                  <  2:2052443/100‑1 (MQ=255)
          cgatctaCCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCa                                                                                  <  2:1936218/94‑1 (MQ=255)
           gATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGTGGCACAGATTCGTCAg                                                                                 >  2:1915861/1‑100 (MQ=255)
            aTTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGAACGCGTGGTTCTGCGGCACAGATTCGTCAg                                                                                 >  1:1095533/1‑99 (MQ=255)
             ttAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGTGGCACAGATTCGTCAGCt                                                                               >  2:1963341/1‑100 (MQ=255)
              tAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGTGGCACAGATTCGTCAGCtt                                                                              >  2:1097966/1‑100 (MQ=255)
              tAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGTGGCACAGATTCGTCAGCtt                                                                              >  1:1541812/1‑100 (MQ=255)
               aaCCGTGACGGTCAGGAAGCGCAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGTGGCACAGATTCGTCAGCTTg                                                                             >  2:80236/1‑100 (MQ=255)
               aaCCGTGACGGCCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCCACATGATCGCCGGCACGGGTGCGCGTGGTCCGGTGTTACAAATACTTCAGCTTg                                                                             >  2:1036430/1‑100 (MQ=255)
                aCCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGTGGCACAGATTCGTCAGCTTGc                                                                            >  1:679115/1‑100 (MQ=255)
                aCCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGTGGCACAGATTCGTCAGCTTGc                                                                            <  2:1425346/100‑1 (MQ=255)
                aCCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCaagatcg                                                                            >  1:1936234/1‑94 (MQ=255)
                   gTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGTGGCACAGATTCGTCAGCTTGCTgn                                                                         <  1:1675163/100‑2 (MQ=255)
                   gTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGTGGCACAGATTCGTCAGCTTGCTgg                                                                         <  2:1323410/100‑1 (MQ=255)
                   gTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGTGGCACAGATTCGTCAGCTTGCTgg                                                                         >  1:448181/1‑100 (MQ=255)
                     gACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGTGGCACAGATTCGTCAGCTTGCTGGTa                                                                       <  2:207604/100‑1 (MQ=255)
                       cGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGTGGCACAGATTCGTCAGCTTGCTGGTATg                                                                     <  2:1790867/100‑1 (MQ=255)
                         gTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGTGGCACAGATTCGTCAGCTTGCTGGTATGCg                                                                   <  1:281256/100‑1 (MQ=255)
                         gTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGTGGCACAGATTCGTCAGCTTGCTGGTATGCg                                                                   <  1:1316943/100‑1 (MQ=255)
                         gTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGTGGCACAGATTCGTCAGCTTGCTGGTATGCg                                                                   >  1:1083284/1‑100 (MQ=255)
                         cgatctaaGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGTGGCACAGATTCGTCAGCTTGCTGGTATGCg                                                                   <  2:454311/94‑1 (MQ=255)
                             ggAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGTGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGt                                                               >  2:588717/1‑100 (MQ=255)
                             ggAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGTGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGt                                                               >  2:1018036/1‑100 (MQ=255)
                              gAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTGAGCTTGCTGGTATGCGTGGTc                                                              >  1:1990884/1‑100 (MQ=255)
                               aaGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGTGGCACAGATTCGTCAGCTTGCTGGTATGCgagatcg                                                             >  1:454315/1‑94 (MQ=255)
                                agagAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGTGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTg                                                            >  1:836869/1‑100 (MQ=255)
                                 gagaAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGTGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGa                                                           <  2:744862/100‑1 (MQ=255)
                                  agaAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGTGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGAt                                                          >  2:1896895/1‑100 (MQ=255)
                                  agaAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGTGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGAt                                                          >  2:917932/1‑100 (MQ=255)
                                  agaAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGTGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGAt                                                          >  2:2067006/1‑100 (MQ=255)
                                   gaAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGTGGCACAGATTCGTCCGCTTGCTGGTATGCGTGGTCTGATg                                                         >  2:388786/1‑100 (MQ=255)
                                   gaAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGTGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATg                                                         <  2:1746464/100‑1 (MQ=255)
                                    aaGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGTGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATgg                                                        <  2:627653/100‑1 (MQ=255)
                                    aaGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGTGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATgg                                                        <  1:1896911/100‑1 (MQ=255)
                                     aGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGTGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGc                                                       >  1:1949816/1‑100 (MQ=255)
                                     aGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGTGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGc                                                       <  1:1744360/100‑1 (MQ=255)
                                      gCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTGCTGTGGCACAGATTCGACAGCTTGCTGGTATGCGTGGTCTGATGGc                                                       <  2:2052324/99‑1 (MQ=255)
                                       cAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGTGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGa                                                     <  2:1813183/100‑1 (MQ=255)
                                        aGGTTTCCTTCAACAGCATCTACATGCTGGCCGACTCCGGTGCGCGTGGTTCTGTGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGa                                                     >  2:1663839/1‑99 (MQ=255)
                                        aGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGAGGAACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGaa                                                    >  2:1664128/1‑100 (MQ=255)
                                         ggTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGTGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAg                                                   >  1:882178/1‑100 (MQ=255)
                                          gTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGTGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGc                                                  >  2:1512263/1‑100 (MQ=255)
                                             tCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGTGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCgg                                               <  2:388562/100‑1 (MQ=255)
                                               cTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGTGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGAt                                             >  1:2002913/1‑100 (MQ=255)
                                               cTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGTGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGAt                                             >  1:1560671/1‑100 (MQ=255)
                                                  cAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGTGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGc                                          >  2:499591/1‑100 (MQ=255)
                                                   aaCAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGTGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCt                                         <  2:944073/100‑1 (MQ=255)
                                                    aCAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGTGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTc                                        >  1:757788/1‑100 (MQ=255)
                                                     cAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGTGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTcc                                       <  2:2000623/100‑1 (MQ=255)
                                                      aGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGTGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCca                                      <  1:153168/100‑1 (MQ=255)
                                                      aGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGTGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCca                                      >  2:144679/1‑100 (MQ=255)
                                                       gCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGTGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCcat                                     <  1:411354/100‑1 (MQ=255)
                                                          tCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGTGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCcatcat                                  <  1:1288407/100‑1 (MQ=255)
                                                          tCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGTGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCcatcat                                  >  1:1244435/1‑100 (MQ=255)
                                                           cTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGTGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCatcatc                                 <  2:506970/100‑1 (MQ=255)
                                                           cTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGTGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCatcatc                                 <  1:1691264/100‑1 (MQ=255)
                                                            tACATGATGGCCGACTCCGGTGCGCGTGGTTCTGTGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCg                                <  1:1815158/100‑1 (MQ=255)
                                                             aCATGATGGCCGACTCCGGTGCGCGTGGTTCTGTGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGa                               >  2:1586030/1‑100 (MQ=255)
                                                             aCATGATGGCCGACTCCGGTGCGCGTGGTTCTGTGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGa                               >  1:1798469/1‑100 (MQ=255)
                                                                  atgGCCGACTCCGGTGCGCGTGGTTCTGTGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGc                          >  1:1873808/1‑100 (MQ=255)
                                                                    ggCCGACTCCGGTGCGCGTGGTTCTGTGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCa                        <  1:859928/100‑1 (MQ=255)
                                                                      ccGACTCCGGTGCGCGTGGTTCTGTGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAAt                      <  1:457166/100‑1 (MQ=255)
                                                                      ccGACTCCGGTGCGCGTGGTTCTGTGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAAt                      >  1:485136/1‑100 (MQ=255)
                                                                      ccGACTCCGGTGCGCGTGGTTCTGTGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAAt                      >  1:939761/1‑100 (MQ=255)
                                                                       gatcTCCGGTGCGCGTGGTTCTGTGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATc                     <  1:1475298/97‑1 (MQ=255)
                                                                       cGACTCCGGTGCGCGTGGTTCTGTGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATc                     <  1:922413/100‑1 (MQ=255)
                                                                          cTCCGGTGCGCGTGGTTCTGTGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCAcn                  <  2:1481989/100‑2 (MQ=255)
                                                                          cTCCGGTGCGCGTGGTTCTGTGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCAcc                  >  2:366615/1‑100 (MQ=255)
                                                                            ccGGTGCGCGTGGTTCTGTGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCagatc                >  2:1475288/1‑96 (MQ=255)
                                                                            ccGGTGCGCGTGGTTCTGTGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACcgc                >  2:958052/1‑100 (MQ=255)
                                                                                      tGGTTCTGTGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGt      >  1:1466906/1‑100 (MQ=255)
                                                                                      tGGTTCTGTGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGt      <  1:1933584/100‑1 (MQ=255)
                                                                                      tGGTTCTGTGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGt      <  1:606972/100‑1 (MQ=255)
                                                                                         ttCTGTGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGaa   <  1:499595/100‑1 (MQ=255)
                                                                                          tCTGTGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAg  <  1:1664565/100‑1 (MQ=255)
                                                                                              |                                                                                               
ACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAG  >  NC_000913/4187456‑4187645

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A