Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F54 I1 R1	44	120.8	6135323	93.2%	5718121	99.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,767,507	(C)_7→8	intergenic (+152/‑203)	rnlB → / → yfjP	CP4‑57 prophage; uncharacterized protein/CP4‑57 prophage; 50S ribosome‑binding GTPase family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,767,500	1	.	C	95.6%	391.0 / 12.0	117	intergenic (+145/‑210)	rnlB/yfjP	CP4‑57 prophage; uncharacterized protein/CP4‑57 prophage; 50S ribosome‑binding GTPase family protein
Reads supporting (aligned to +/- strand): ref base . (2/3); new base C (52/56); total (57/60)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.30e-01

Only 100 of 131 total aligned reads displayed.

TGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCTCACAACGTC‑CTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTT‑‑CCCCCCC‑TTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCT  >  NC_000913/2767402‑2767594

tGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCTCACAACGTC‑CTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTT‑‑c                                                                                                <  2:2324301/100‑1 (MQ=255)

  atatTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCTCACAACGTC‑CTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTT‑‑ccc                                                                                              <  2:2252083/100‑1 (MQ=255)

  atatTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCTCACAACGTC‑CTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTT‑‑ccc                                                                                              <  2:3008624/100‑1 (MQ=255)

   tatTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCTCACAACGTG‑CCGGCTCAGGAAAACGTCTTCCCCAGTACACCCCCGACCGTATTT‑‑cccc                                                                                             >  2:1260529/1‑100 (MQ=255)

    atTAACTTGTCCAATATGATCAAATAGCATTAACTCCCCCTCACAATATA‑CTGCATAAGGAACACATATTCCCCTGTGCACCCACGACTAAATTT‑‑ccc                                                                                              >  1:2223171/1‑98 (MQ=255)

     ttAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCTCACAACGTC‑CTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTT‑‑cccccc                                                                                           <  2:971509/100‑1 (MQ=255)

     ttAACTTGTCCAATATGATCAAATAGCATTAACACCCGCTCAAAGCGTC‑GTGCAGAGGAGCCACGTTATCGACAGAGCACCAAAGACTAAAGTT‑‑ccacca                                                                                           >  2:1167476/1‑99 (MQ=255)

       aaCTTGTCCAATATGATCAAATAGCATTAACCCCCCCTCACAACGTC‑CTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTT‑‑cccccccc                                                                                         <  2:2995909/100‑2 (MQ=255)

           tGTCCAATATGATCAAATAGCATTAACCCCCCCTCCCAACGTC‑CTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTT‑‑CCCCCCC‑ctt                                                                                      <  1:70545/98‑1 (MQ=255)

              ccAATATGATCAAATAGCATTAACCCCCCCTCACAACGTC‑CTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTC‑CCCCCCC‑TTTAAAc                                                                                  <  2:2514851/100‑1 (MQ=255)

              ccAATATGATCAAATAGCATTAACCCCCCCTCACAACGTC‑CTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTC‑CCCCCCC‑TTTAAAc                                                                                  <  2:417735/100‑1 (MQ=255)

              ccAATATGATCAAATAGCATTAACCCCCCCTCACAACGTC‑CTGCATAGGGAACACATTTTCCCCTGTGCACAAACGACTACATAT‑‑cccaaatcccaa                                                                                     >  2:2095921/1‑88 (MQ=255)

              ccAATATGATCAAATAGCATTAACACCCCCTCCCAA‑GTCACTGCATAGGACTCCCCCTTTCCCCTGTGCATACACAACTAACTTTA‑CCTTCCC‑TTTaact                                                                                  >  1:2262983/1‑98 (MQ=255)

                   atGATCAAATAGCATTAACCCCCCCTCACAACGTC‑CTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTC‑CCCCCCC‑TTTAAACTtctc                                                                             <  1:1013660/100‑1 (MQ=255)

                    tGATCAAATAGCATTAACCCCCCCTCACAACGTC‑CTGCATAAGCAACACGTTTCCCCCTGTGCATCCACGCCCAAATTTC‑CCCCCCC‑TTTAAACTtctc                                                                             >  1:2822159/1‑99 (MQ=255)

                     gATCAAATAGCATTAACCCCCCCTCACAACGTC‑CTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTC‑CCCCCCC‑TTTAAACTTCTCaa                                                                           <  2:2634571/100‑1 (MQ=255)

                     gATCAAATAGCATTAACCCCCCCTCACAACGTC‑CTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTC‑CCCCCCC‑TTTAAACTTCTCaa                                                                           <  1:3058832/100‑1 (MQ=255)

                           aTAGCATTAACCCCCCCTCACAACGTC‑CTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTC‑CCCCCCC‑TTTAAACTTCTCAATAATGt                                                                     <  2:296831/100‑1 (MQ=255)

                            tAGCATTAACCCCCCCTCACAACGTC‑CTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTC‑CCCCCCC‑TTTAAACTTCTCAATAATGTc                                                                    <  2:3039757/100‑1 (MQ=255)

                              gCATTAACCCCCCCTTACAACGTC‑CTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTC‑CCCCCCC‑TTTAAACTTCTCAATAATGTCAc                                                                  <  2:3009636/100‑1 (MQ=255)

                                aTTAACCCCCCCTCACAACGTC‑CTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTC‑CCCCCCC‑TTTAAACTTCTCAATAATGTCACCa                                                                <  2:1155251/100‑1 (MQ=255)

                                     cccccgcTGACAACGTC‑CTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTC‑CCCCCCC‑TTTTAACTTCTCAATTACGGCACCAAtttt                                                           >  1:310509/1‑100 (MQ=255)

                                        ccccTCACAACGTC‑CTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTC‑CCCCCCC‑TTTAAACTTCTCAATAATGTCACCAATTTTCTg                                                        <  2:1870040/100‑1 (MQ=255)

                                        ccccTCACAACGTC‑CTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTC‑CCCCCCC‑TTTAAACTTCTCAATAATGTCACCAATTTTCTg                                                        <  2:495383/100‑1 (MQ=255)

                                          ccTCACAACGTC‑CTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTC‑CCCCCCC‑TTTAAACTTCTCAATAATGTCACCAATTTTCTGGa                                                      <  2:458724/100‑1 (MQ=255)

                                           cTCACAACGTC‑CTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTC‑CCCCCCC‑TTTAAACTTCTCAATAATGTCACCAATTTTCTGGaa                                                     >  1:1616923/1‑100 (MQ=255)

                                            tCACAACGTC‑CTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTC‑CCCCCCC‑TTTAAACTTCTCAATAATGTCACCAATTTTCTGGaaa                                                    >  2:87715/1‑100 (MQ=255)

                                             cacaACGTC‑CTGCATAGGGAACACGTTTTCCCCTGTGGACCCACGACTAAATTTC‑CCCCCCC‑TTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAAt                                                   >  1:1640276/1‑100 (MQ=255)

                                              acaACGTC‑CTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTC‑CCCCCCC‑TTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATc                                                  <  2:650520/100‑1 (MQ=255)

                                               caACGTC‑CTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTC‑CCCCCCC‑TTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATcc                                                 <  2:551181/100‑1 (MQ=255)

                                                aaCGTC‑CTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTTAATTTC‑CCCCCCC‑TTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCt                                                <  1:18277/100‑1 (MQ=255)

                                                 aCGTC‑CTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTTAATTTC‑CCCCCCC‑TTTAAACTTCTCAATTATGTCACCAATTTTCTGGAAATCCTa                                               <  1:1193962/100‑1 (MQ=255)

                                                  cGTC‑CTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTC‑CCCCCCC‑TTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTat                                              >  1:2500297/1‑100 (MQ=255)

                                                  cGTC‑CTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAACTTC‑CCCCCCC‑TTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTa                                               <  2:2635767/99‑1 (MQ=255)

                                                   gatcttGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTC‑CCCCCCC‑TTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTat                                              <  2:1796090/95‑1 (MQ=255)

                                                   gTC‑CTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTC‑CCCCCCC‑TTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTatg                                             >  1:3011688/1‑100 (MQ=255)

                                                    tC‑CTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTC‑CCCCCCC‑TTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTatga                                            <  2:88025/100‑1 (MQ=255)

                                                       cTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTC‑CCCCCCC‑TTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTatgatg                                          >  1:1683206/1‑100 (MQ=255)

                                                       cTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTC‑CCCCCCC‑TTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTatgatg                                          >  1:541166/1‑100 (MQ=255)

                                                       cTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTC‑CCCCCCC‑TTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTatg                                             <  1:1381274/97‑1 (MQ=255)

                                                       cTGCATAGCGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTC‑CCCCCCC‑TTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTatgatg                                          <  1:2808878/100‑1 (MQ=255)

                                                        tGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTC‑CCCCCCC‑TTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGc                                         >  1:391664/1‑100 (MQ=255)

                                                        tGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTC‑CCCCCCC‑TTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGc                                         <  2:392909/100‑1 (MQ=255)

                                                        tGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTC‑CCCCCCC‑TTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGc                                         >  2:36802/1‑100 (MQ=255)

                                                        tGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTC‑CCCCCCC‑TTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGc                                         <  1:2951075/100‑1 (MQ=255)

                                                        tGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTC‑CCCCCCC‑TTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGc                                         <  2:333752/100‑1 (MQ=255)

                                                        tGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTA‑CCCCCCC‑TTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGc                                         <  1:2951846/100‑1 (MQ=255)

                                                         gCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTC‑CCCCCCC‑TTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGcc                                        <  2:268694/100‑1 (MQ=255)

                                                         gCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTC‑CCCCCCC‑TTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGcc                                        <  2:268972/100‑1 (MQ=255)

                                                            tAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTC‑CCCCCCC‑TTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCttt                                     >  1:457907/1‑100 (MQ=255)

                                                            tAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTC‑CCCCCCC‑TTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCttt                                     >  2:929004/1‑100 (MQ=255)

                                                             aGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTC‑CCCCCCC‑TTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTc                                    >  1:1060917/1‑100 (MQ=255)

                                                             aGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTC‑CCCCCCC‑TTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTc                                    >  2:1564978/1‑100 (MQ=255)

                                                             aGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTC‑CCCCCCC‑TTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTc                                    >  1:1802376/1‑100 (MQ=255)

                                                              gggAACACGTTTTCCCCTGTGCACCCACGACTAAATTTC‑CCCCCCC‑TTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTcc                                   >  2:1978522/1‑100 (MQ=255)

                                                              gggAACACGTTTTCCCCTGTGCACCCACGACTAAATTTC‑CCCCCCC‑TTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTcc                                   >  2:2579551/1‑100 (MQ=255)

                                                              gggAACACGTTTTCCCCTGTGCACCCACGACTAAATTTC‑CCCCCCC‑TTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTcc                                   >  2:137128/1‑100 (MQ=255)

                                                               ggAACACGTTTTCCCCTGTGCACCCACGACTAAATTTC‑CCCCCCC‑TTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTccc                                  >  1:128763/1‑100 (MQ=255)

                                                               ggAACACGTTTTCCCCTGTGCACCCACGACTAAATTTC‑CCCCCCC‑TTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTccc                                  <  1:927559/100‑1 (MQ=255)

                                                                gAACACGTTTTCCCCTGTGCACCCACGACTAAATTGCCCCCCCCC‑TTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTccc                                  <  1:517955/100‑1 (MQ=255)

                                                                gAACACGTTTGCCCCTGTGCACCCACGACTAATTTTC‑CCCCCCC‑TTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCg                                 <  2:778763/100‑1 (MQ=255)

                                                                 aaCACGTTTTCCCCTGTGCACCCACGACTAAATTTC‑CCCCCCC‑TTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCgg                                <  2:2670760/100‑1 (MQ=255)

                                                                  acacGTTTTCCCCTGTGCACCCACGACTAAATTTC‑CCCCCCC‑TTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGt                               >  2:1777962/1‑100 (MQ=255)

                                                                   cacGTTTTCCCCTGTGCACCCACTACTAAATTTC‑CCCCCCC‑TTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTa                              <  1:2920367/100‑1 (MQ=255)

                                                                   cacGTTTTCCCCTGTGCACCCACGACTAGATTTC‑CCCCCCC‑TTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTa                              >  2:466556/1‑100 (MQ=255)

                                                                   cacGTTTTCCCCTGTGCACCCACGACTAAATTTC‑CCCCCCC‑TTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCgg                                >  1:2674262/1‑98 (MQ=255)

                                                                     cattttCCCCTGTGCACCCACGACTAAATTTC‑CCCCCCC‑TTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTaaa                            >  2:2063977/3‑100 (MQ=255)

                                                                     cGTTTTCCCCTGTGCACCCACGACTAAATTTC‑CCCCCCC‑TTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTaag                            >  1:174510/1‑99 (MQ=255)

                                                                     cGTTTTCCCCTGTGCACCCACGACTAAATTTC‑CCCCCCC‑TTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTaaa                            >  1:1964439/1‑100 (MQ=255)

                                                                      gTTTTCCCCTGTGCACCCTCGACTAAATTTC‑CCCCCCC‑TTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAAt                           <  2:2095573/100‑1 (MQ=255)

                                                                      gTTTTCCCCTGTGCACCCACGACTAAATTTC‑CCCCCCC‑TTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAAt                           >  1:689133/1‑100 (MQ=255)

                                                                       ttttCCCCTGTGCACCCACGACTAAATTT‑‑CCCCCCCATTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATg                          <  2:2151438/100‑1 (MQ=255)

                                                                         ttCCCCTGTGCACCCACGACTAAATTTC‑CCCCCCC‑TTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGACGCCTTTCCCGGTAAATGGt                        <  2:78021/100‑1 (MQ=255)

                                                                          tCCCCTGTGCACCCACGACTAAATTTC‑CCCCCCC‑TTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTg                       <  2:1693773/100‑1 (MQ=255)

                                                                           ccccTGTGCACCCACGACTAAATTTC‑CCCCCCC‑TTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGc                      <  1:2551468/100‑1 (MQ=255)

                                                                             ccTGTGCACCCACGACTAAATTTC‑CCCCCCC‑TTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCtg                    >  2:2235934/1‑100 (MQ=255)

                                                                                gtgCACCCACGACTAAATTTC‑CCCCCCC‑TTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGa                 >  1:1101470/1‑100 (MQ=255)

                                                                                  gCACCCACGACTAAATTT‑‑CCCCCCC‑TTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGtt              >  2:2629368/1‑100 (MQ=255)

                                                                                   cACCCACGACTAAATTTC‑CCCCCCC‑TTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGa                 >  1:265540/1‑97 (MQ=255)

                                                                                   cACCCACGACTAAATTTC‑CCCCCCC‑TTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAgn               <  2:1385477/99‑2 (MQ=255)

                                                                                    aCCCACGACTAAATTTC‑CCCCCCC‑TTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTc             <  2:170353/100‑1 (MQ=255)

                                                                                     cccATGATTATATTTC‑CCCCCCC‑TCTTAACTTCTCAATAAGGTCACCATTTTTCTGGAAATACTATGATGCCTTTCCCGGTAAATGGTACTGTGAGTTCa            <  1:325904/100‑1 (MQ=255)

                                                                                     cccACGACTAAATTTC‑CCCCCCC‑TTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCa            >  2:2524730/1‑100 (MQ=255)

                                                                                     cccACGACTAAATTTC‑CCCCCCC‑TTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCa            >  2:1270257/1‑100 (MQ=255)

                                                                                      ccACGACTAAATTTC‑CCCCCCC‑TTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAg           >  2:1237888/1‑100 (MQ=255)

                                                                                       cACGACTAAATTTC‑CCCCCCC‑TTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGa          >  2:2997850/1‑100 (MQ=255)

                                                                                       cACGACTAAATTTC‑CCCCCCC‑TTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGa          >  2:1984148/1‑100 (MQ=255)

                                                                                         cGACTAAATTTC‑CCCCCCC‑TTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGaaa        >  1:90570/1‑100 (MQ=255)

                                                                                         cGACTAAATTTC‑CCCCCCC‑TTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGaaa        >  1:1251363/1‑100 (MQ=255)

                                                                                         cGACTAAATTTC‑CCCCCCC‑TTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGaaa        >  1:2791410/1‑100 (MQ=255)

                                                                                         cGACTAAATTTC‑CCCCCCC‑TTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGaaa        >  1:2264792/1‑100 (MQ=255)

                                                                                          gACTAAATTTC‑CCCCCCC‑TTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAg       >  2:2842362/1‑100 (MQ=255)

                                                                                           aCTAAATTTC‑CCCCCCC‑TTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAg       <  1:1739489/99‑1 (MQ=255)

                                                                                           aCTAAATTTC‑CCCCCCC‑TTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGa      <  2:312848/100‑1 (MQ=255)

                                                                                            cTAAATTTC‑CCCCCCC‑TTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGAt     <  1:2063969/100‑1 (MQ=255)

                                                                                            cTAAATTTC‑CCCCCCC‑TTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGAt     >  1:2475646/1‑100 (MQ=255)

                                                                                            cTAAATTTC‑CCCCCCC‑TTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGAt     >  1:2593711/1‑100 (MQ=255)

                                                                                              aaaTTTC‑CCCCCCC‑TTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTc   >  2:1818523/1‑100 (MQ=255)

                                                                                              aaaTTTC‑CCCCCCC‑TTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTc   >  1:1732280/1‑100 (MQ=255)

                                                                                              aaaTTTC‑CCCCCCC‑TTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTc   >  2:1415428/1‑100 (MQ=255)

TGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCTCACAACGTC‑CTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTT‑‑CCCCCCC‑TTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCT  >  NC_000913/2767402‑2767594

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A