Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F54 I1 R1 44 120.8 6135323 93.2% 5718121 99.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,008,075 A→G D189G (GAC→GGC)  ygeX → 2,3‑diaminopropionate ammonia lyase, PLP‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,008,0750AG97.8% 327.5 / ‑4.6 92D189G (GAC→GGC) ygeX2,3‑diaminopropionate ammonia lyase, PLP‑dependent
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base G (41/49);  total (42/50)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGGTGCCGAGTGCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGACACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAGATGAAGCCGTCGAGCAAATGCGTGAAATGGG  >  NC_000913/3007980‑3008171
                                                                                               |                                                                                                
cGGTGCCGAGTGCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACg                                                                                              <  2:2709839/100‑1 (MQ=255)
cGGTGCCGAGTGCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACg                                                                                              >  1:665602/1‑100 (MQ=255)
     ccGAGTGCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATg                                                                                         <  1:493947/100‑1 (MQ=255)
      cGAGTGCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATgg                                                                                        >  2:1492900/1‑100 (MQ=255)
      cGAGTGCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATgg                                                                                        <  2:795237/100‑1 (MQ=255)
      cGAGTGCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATgg                                                                                        <  2:795345/100‑1 (MQ=255)
       gAGTGCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATggg                                                                                       <  2:2991987/100‑1 (MQ=255)
           gCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACATGCGCAGCAGCACGGCTGGGAAGTGGTACAGAGCACGGCATGGGAAg                                                                                    >  1:1796903/1‑99 (MQ=255)
           gCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAgg                                                                                   <  2:511750/100‑1 (MQ=255)
            cATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGt                                                                                  >  1:1223909/1‑100 (MQ=255)
            cATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGt                                                                                  <  2:79602/100‑1 (MQ=255)
             aTCGTCACGGATATGAACTATGACGATACCTTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGt                                                                                  <  1:2412796/99‑1 (MQ=255)
             aTCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGtt                                                                                 >  2:709343/1‑100 (MQ=255)
                gTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTaca                                                                              <  2:344650/100‑1 (MQ=255)
                gTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTaca                                                                              >  2:2508615/1‑100 (MQ=255)
                gTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTaca                                                                              >  1:2544199/1‑100 (MQ=255)
                 tCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTacac                                                                             >  1:188915/1‑100 (MQ=255)
                  cACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACAcc                                                                            <  2:2991571/100‑1 (MQ=255)
                     ggATATGAACTATGACGATACCGTTCGCCGGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCaaa                                                                         <  1:2303529/100‑1 (MQ=255)
                       atatGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAAt                                                                       <  2:112158/100‑1 (MQ=255)
                       atatGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAAt                                                                       <  2:2211315/100‑1 (MQ=255)
                         atGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATcc                                                                     >  2:2453282/1‑100 (MQ=255)
                         atGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATcc                                                                     <  2:896421/100‑1 (MQ=255)
                         atGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATcc                                                                     <  2:2560501/100‑1 (MQ=255)
                          tGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATccc                                                                    >  2:1840246/1‑100 (MQ=255)
                          tGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATccc                                                                    >  1:1144956/1‑100 (MQ=255)
                           gAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCa                                                                   >  2:2314431/1‑100 (MQ=255)
                             aCTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCaa                                                                  <  2:2554679/99‑1 (MQ=255)
                              cTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACCGGGCACGGCATGGGAAGGTTACACCAAAATCCCAAcc                                                                <  1:1648593/100‑1 (MQ=255)
                                 tGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTgg                                                             >  2:515299/1‑100 (MQ=255)
                                  gACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGa                                                            <  1:3029086/100‑1 (MQ=255)
                                    cGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAATATCCCAACCTGGATc                                                          <  1:924213/100‑1 (MQ=255)
                                    cGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGACACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATc                                                          >  1:2754449/1‑100 (MQ=255)
                                     gATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCa                                                         <  2:2811206/100‑1 (MQ=255)
                                     gATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCa                                                         <  2:2292705/100‑1 (MQ=255)
                                      aTACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCAt                                                        <  2:1728451/100‑1 (MQ=255)
                                       tACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATg                                                       >  2:1774620/1‑100 (MQ=255)
                                       tACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATg                                                       <  1:737773/100‑1 (MQ=255)
                                         ccGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCa                                                     >  2:1852767/1‑100 (MQ=255)
                                          cGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCaa                                                    >  1:560514/1‑100 (MQ=255)
                                           gTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAg                                                   <  2:1991255/100‑1 (MQ=255)
                                           gTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAg                                                   <  1:1653516/100‑1 (MQ=255)
                                              cGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCt                                                <  1:1072362/100‑1 (MQ=255)
                                               gCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTa                                               >  2:630231/1‑100 (MQ=255)
                                                ccTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTAc                                              >  1:932826/1‑100 (MQ=255)
                                                ccTGACCATGCAACACGCGCAGCAGCACGCCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTAc                                              <  1:1840239/100‑1 (MQ=255)
                                                 cTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACg                                             >  1:1298012/1‑100 (MQ=255)
                                                  tGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGc                                            <  2:281994/100‑1 (MQ=255)
                                                  tGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGc                                            <  2:1840785/100‑1 (MQ=255)
                                                  tGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGc                                            <  1:2188594/100‑1 (MQ=255)
                                                  tGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACAAAAATCCCAACCTGGATCATGCAAGGCTACGc                                            >  1:1069145/1‑100 (MQ=255)
                                                   gACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCa                                           >  1:2501295/1‑100 (MQ=255)
                                                   gACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCa                                           >  1:1488443/1‑100 (MQ=255)
                                                   atctaTGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCa                                           <  1:1594509/96‑1 (MQ=255)
                                                     ccATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAAc                                         >  1:349033/1‑100 (MQ=255)
                                                     ccATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACACAAc                                         <  1:709342/100‑1 (MQ=255)
                                                       aTGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCaggat                                       >  2:1594515/1‑96 (MQ=255)
                                                       aTGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAAccc                                       <  2:1469944/100‑1 (MQ=255)
                                                       aTGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAAccc                                       <  2:400826/100‑1 (MQ=255)
                                                       aTGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGATACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAAccc                                       <  2:356376/100‑1 (MQ=255)
                                                        tGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAAcc                                        >  1:2869223/1‑98 (MQ=255)
                                                         gCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTg                                     <  1:2413027/100‑1 (MQ=255)
                                                         gCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGACACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTg                                     <  1:2559210/100‑1 (MQ=255)
                                                           aaCACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGc                                   >  2:166397/1‑100 (MQ=255)
                                                           aaCACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGc                                   <  2:1144960/100‑1 (MQ=255)
                                                            acacGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTgg                                    <  1:2328924/98‑1 (MQ=255)
                                                             cacGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAg                                 >  2:597633/1‑100 (MQ=255)
                                                             cacGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAg                                 >  1:909558/1‑100 (MQ=255)
                                                             cacGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAg                                 <  1:597632/100‑1 (MQ=255)
                                                             cacGCGCAGCAGCACGGCTGGGAAGCGGTACAGGGCACGGCCAGGGAAGGTTCCACCACAATCCCAACCTGGACCATGCAAGGCTACGCAACCCTCGc                                   >  1:895451/1‑98 (MQ=255)
                                                               cgcgCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAGAt                               >  1:1152121/1‑100 (MQ=255)
                                                               cgcgCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAGAt                               <  2:349034/100‑1 (MQ=255)
                                                                 cgcAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAGATGa                             <  1:2758081/100‑1 (MQ=255)
                                                                 cgcAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAGATGa                             <  2:927181/100‑1 (MQ=255)
                                                                 cgcAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAGATGa                             <  2:2358428/100‑1 (MQ=255)
                                                                 cgcAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAGATGa                             >  2:2266661/1‑100 (MQ=255)
                                                                    agcagcaCGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGGTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAGATGaa                            <  1:1034160/98‑1 (MQ=255)
                                                                          aCGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAGATGAAGCCGTCGa                    >  2:2579629/1‑100 (MQ=255)
                                                                           cGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAGATGAAGCCGTCGAg                   <  1:1492895/100‑1 (MQ=255)
                                                                            ggCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAGATGAAGCCGTCGAGc                  <  2:2438493/100‑1 (MQ=255)
                                                                             gCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAGATGAAGCCGTCGAGCa                 >  2:1011959/1‑100 (MQ=255)
                                                                              cTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAGATGAAGCCGTCGAGCa                 >  1:2859996/1‑99 (MQ=255)
                                                                              cTGGGAAGTGGTACAGGGCACGGCATGAGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAGATGAAGCCGTCGAGCa                 <  1:868899/99‑1 (MQ=255)
                                                                                 ggAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAGATGAAGCCGTCGAGCaaa               >  1:1103978/1‑98 (MQ=255)
                                                                                     gTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAGATGAAGCCGTCGAGCAAATGCGTg         >  2:1783290/1‑100 (MQ=255)
                                                                                       ggTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAGATGAAGCCGTCGAGCAAATGCGTGaa       >  2:2538298/1‑100 (MQ=255)
                                                                                       ggTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAGATGAAGCCGTCGAGCAAATGCGTGaa       >  2:1962122/1‑100 (MQ=255)
                                                                                        gTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAGATGAAGCCGTCGAGCAAATGCGTGaaa      >  2:2135444/1‑100 (MQ=255)
                                                                                        gTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAGATGAAGCCGTCGAGCAAATGCGTGaaa      <  1:1774613/100‑1 (MQ=255)
                                                                                           cAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAGATGAAGCCGTCGAGCAAATGCGTGAAATgg   >  2:1449750/1‑100 (MQ=255)
                                                                                           cAGGGCACGACATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAGATGAAGCCGTCGAGCAAATGCGTGAAATgg   >  1:1005626/1‑100 (MQ=255)
                                                                                            aGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAGATGAAGCCGTCGAGCAAATGCGTGAAATggg  <  2:2542931/100‑1 (MQ=255)
                                                                                               |                                                                                                
CGGTGCCGAGTGCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGACACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAGATGAAGCCGTCGAGCAAATGCGTGAAATGGG  >  NC_000913/3007980‑3008171

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A