Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A10 F44 I0 R1 58 156.1 7762724 94.5% 7335774 99.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,189,448 C→T 100% Q1367* (CAG→TAG)  rpoC → RNA polymerase, beta prime subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,189,4480CT96.2% 501.7 / 5.4 160Q1367* (CAG→TAG) rpoCRNA polymerase, beta prime subunit
Reads supporting (aligned to +/- strand):  ref base C (5/1);  new base T (73/80);  total (78/82)
Fisher's exact test for biased strand distribution p-value = 1.12e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.26e-01

Only 100 of 165 total aligned reads displayed.

AGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCTGCCAGCCTGGCAGAACTGCTGAAC  >  NC_000913/4189351‑4189543
                                                                                                 |                                                                                               
 gcagcCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAgg                                                                                              <  2:103887/100‑1 (MQ=255)
 gcagcCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAgg                                                                                              <  1:3500757/100‑1 (MQ=255)
 gcagcCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAgg                                                                                              <  2:2467020/100‑1 (MQ=255)
   agcCGTTGCGGGCAAACGCGGCGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGa                                                                                             <  1:3770834/99‑1 (MQ=255)
    gcCGTTGCGGGTAAACGCGACGCACTGCGCGGCCTGAAAGAGAAAGTGATCGGGGGTCGTCTGATCTCGGAAGGGACCGGTTACGCGTACCACTAGGATc                                                                                           >  2:785008/1‑100 (MQ=255)
      cGTTGCGGGCAAGCGGGACGAACTGCGCGGCCTGAAAGAGGACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGt                                                                                         <  2:1678263/100‑1 (MQ=255)
       gTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTa                                                                                        >  2:329669/1‑100 (MQ=255)
        ttGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTAt                                                                                       <  1:612675/100‑1 (MQ=255)
           cGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGCACGTTATCGTGGGTCGTCTGGTCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCg                                                                                    >  2:2265868/1‑100 (MQ=255)
            gggCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGt                                                                                   >  2:1390134/1‑100 (MQ=255)
            gggCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGt                                                                                   >  2:3208648/1‑100 (MQ=255)
             ggCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTc                                                                                  >  1:1844405/1‑100 (MQ=255)
             ggCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTc                                                                                  <  1:2020120/100‑1 (MQ=255)
             ggCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTc                                                                                  >  2:1341682/1‑100 (MQ=255)
               cAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGc                                                                                >  2:1420520/1‑100 (MQ=255)
                aaaCGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGcc                                                                               >  2:397011/1‑100 (MQ=255)
                 aaCGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATGGTATGCGTCGCCg                                                                              >  2:2256453/1‑100 (MQ=255)
                 aaCGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGCCg                                                                              >  1:1447545/1‑100 (MQ=255)
                   cgcgACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCg                                                                              >  1:232659/1‑98 (MQ=255)
                   cgcgACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGtg                                                                            <  1:1290591/100‑1 (MQ=255)
                     cgacgaACTGCGCGTCGTGAAAGAGAACGTTATCGT‑GGTCGTCTGATCACGGCAGGTAACGGTTAGGCGTATCACTAGTATCGTATGGGTTGCCGtgctg                                                                         >  1:278234/1‑100 (MQ=255)
                     cgacgaACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGtgct                                                                          >  1:1710489/1‑100 (MQ=255)
                     cgacgaACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGtgct                                                                          <  2:2790268/100‑1 (MQ=255)
                        cgaACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCg                                                                       >  2:3120070/1‑100 (MQ=255)
                        cgaACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCg                                                                       <  2:1551697/100‑1 (MQ=255)
                        cgaACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCATATGCGTCGCCGTGCTGCg                                                                       >  1:2532592/1‑100 (MQ=255)
                          aaCTGCGCGGCCTGAACGCGCAAGTTCTCGTGGGTCGTCTGATCCAGGCAGGTACCGGTTACGCGTCCCTTTACGATCGTATGAGTAGCCGTGTTGCggg                                                                     <  1:3236095/100‑1 (MQ=255)
                          aaCTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCggg                                                                     <  1:1114071/100‑1 (MQ=255)
                          aaCTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCggg                                                                     >  2:1810784/1‑100 (MQ=255)
                          aaCTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCggg                                                                     >  1:2430672/1‑100 (MQ=255)
                           aCTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGt                                                                    >  2:2024175/1‑100 (MQ=255)
                           aCTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGt                                                                    <  1:1301866/100‑1 (MQ=255)
                           aCTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGt                                                                    <  1:669776/100‑1 (MQ=255)
                             tGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGa                                                                  >  2:525333/1‑100 (MQ=255)
                              gcgcGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGaa                                                                 <  2:218619/100‑1 (MQ=255)
                              gcgcGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGaa                                                                 <  1:1204393/100‑1 (MQ=255)
                                gcgGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGc                                                               <  1:409279/100‑1 (MQ=255)
                                   gCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTcc                                                            <  2:1729473/100‑1 (MQ=255)
                                     cTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTATGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCgg                                                          >  1:2862744/1‑100 (MQ=255)
                                      tGAATGAGAATGTGATCGGGGGTCGTCTGATCCCGGCAGGTACGGGTTACGCGTACCACTTGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTcc                                                            >  1:2110125/1‑97 (MQ=255)
                                      tGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGc                                                         >  2:1466054/1‑100 (MQ=255)
                                      tGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGc                                                         >  2:297574/1‑100 (MQ=255)
                                       gAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCt                                                        <  2:2700236/100‑1 (MQ=255)
                                         aaGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGc                                                      >  2:2355717/1‑100 (MQ=255)
                                          agagAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCa                                                     >  1:2599999/1‑100 (MQ=255)
                                          agagAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCa                                                     <  2:1954788/100‑1 (MQ=255)
                                          agagAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCa                                                     <  2:1118865/100‑1 (MQ=255)
                                           gagaACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCAc                                                    <  2:3382475/100‑1 (MQ=255)
                                             gaACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCg                                                  >  1:148038/1‑100 (MQ=255)
                                              aaCGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGc                                                 <  2:2446903/100‑1 (MQ=255)
                                              aaCGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGc                                                 >  2:3684202/1‑100 (MQ=255)
                                                cGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAg                                               <  2:1225677/100‑1 (MQ=255)
                                                cGTTATCGTGGGTCGTCTGATCCCGGAAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAg                                               >  2:2357646/1‑100 (MQ=255)
                                                 gTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAgg                                              <  2:3514738/100‑1 (MQ=255)
                                                  ttATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGt                                             >  2:2744759/1‑100 (MQ=255)
                                                   tATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTg                                            <  2:693774/100‑1 (MQ=255)
                                                   tATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTg                                            <  2:1877747/100‑1 (MQ=255)
                                                    aTCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCCGCGGGTGAAGCTCCGGCTGCACCGCAGGt                                             <  1:1956396/98‑1 (MQ=255)
                                                      cGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACt                                         >  2:1468868/1‑100 (MQ=255)
                                                        tGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGc                                       >  2:854945/1‑100 (MQ=255)
                                                        tGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGc                                       <  2:1913805/100‑1 (MQ=255)
                                                        tGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGc                                       <  2:1866318/100‑1 (MQ=255)
                                                        tGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGc                                       <  2:2511077/100‑1 (MQ=255)
                                                             cgtcTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCagaag                                  <  2:3734372/100‑1 (MQ=255)
                                                              gtcTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCagaaga                                 >  2:1177546/1‑100 (MQ=255)
                                                               tcTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGAc                                <  2:492931/100‑1 (MQ=255)
                                                                cTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACg                               >  1:3298220/1‑100 (MQ=255)
                                                                cTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCACCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACg                               >  1:1766504/1‑100 (MQ=255)
                                                                 tGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGc                              >  1:3808263/1‑100 (MQ=255)
                                                                  gATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCa                             >  2:2700589/1‑100 (MQ=255)
                                                                   aTCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCAt                            >  1:379910/1‑100 (MQ=255)
                                                                    tCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATc                           <  1:412496/100‑1 (MQ=255)
                                                                     cccGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCt                          >  2:1310761/1‑100 (MQ=255)
                                                                      ccGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCTg                         <  2:2758563/100‑1 (MQ=255)
                                                                      ccGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCCCCGGCTGCACCGCAGGTGACTGCCGCAGACGCATCTg                         >  1:3377672/1‑100 (MQ=255)
                                                                        ggCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCTGcc                       <  1:3588425/100‑1 (MQ=255)
                                                                         gCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCTGCCa                      <  2:2766068/100‑1 (MQ=255)
                                                                          cAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCTGCCAg                     <  1:2151584/100‑1 (MQ=255)
                                                                          cAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCTGCCAg                     <  2:322183/100‑1 (MQ=255)
                                                                            ggTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCTGCCAGcc                   <  2:2290414/100‑1 (MQ=255)
                                                                            ggTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCTGCCAGcc                   >  2:1097245/1‑100 (MQ=255)
                                                                              tACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCTGCCAGCCTg                 <  2:708687/100‑1 (MQ=255)
                                                                               aCCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCTGCCAGCCTgg                <  1:1390126/100‑1 (MQ=255)
                                                                                ccGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGATGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCTGCCAGCCTGGc               <  1:297572/100‑1 (MQ=255)
                                                                                ccGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCTGCCAGCCTGGc               >  1:1235012/1‑100 (MQ=255)
                                                                                 cGGTTACGCGTACCACTATGTTCGTATGCGGCGCCGTGCTGCGGGTGAAGCTCCGGCGGCACCGCAGGTGACTGCAGAAGACGCATCTGCCAg                     <  1:699044/93‑1 (MQ=255)
                                                                                  ggTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCTGCCAGCCTGGCAg             <  1:3296789/100‑1 (MQ=255)
                                                                                  ggTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCTGCCAGCCTGGCAg             >  1:863619/1‑100 (MQ=255)
                                                                                  ggTTACGCGTACCACTAGGATCATATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCTGCCAGCCTGGCAg             <  2:2532610/100‑1 (MQ=255)
                                                                                   gTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCTGCCAGCCTGGCAGa            >  1:2907041/1‑100 (MQ=255)
                                                                                     tACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCTGCCAGCCTGGCAGAAc          <  2:3808295/100‑1 (MQ=255)
                                                                                       cgcgTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGCCTGCAGAAGACGCATCTGCCAGCCTGGCAGAActg        >  1:911358/1‑100 (MQ=255)
                                                                                        gcgTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGCAGCTCTGGCTGCACCGTAGGTGACTGCAGAAGACGCATCTGCCAGCCTGGCAg             <  1:542797/94‑1 (MQ=255)
                                                                                         cgTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCTGCCAGCCTGGCAGAActgct      <  2:1376413/100‑1 (MQ=255)
                                                                                          gTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTCAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCTGCCAGCCTGGCAGAActgctg     >  1:2072011/1‑100 (MQ=255)
                                                                                            accactAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCTGCCAGCCTGGCAGAACTGCTGaa   <  1:3066296/100‑1 (MQ=255)
                                                                                            accactAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCTGCCAGCCTGGCAGAACTGCTGaa   >  2:1567502/1‑100 (MQ=255)
                                                                                            accactAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCTGCCAGCCTGGCAGAACTGCTGaa   >  1:2326988/1‑100 (MQ=255)
                                                                                            accactAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCTCCCAGCCTGGCAGAACTGCTGaa   <  1:2256438/100‑1 (MQ=255)
                                                                                             ccactaGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCTGCCAGCCTGGCAGAACTGCTGAAc  >  2:2048611/1‑100 (MQ=255)
                                                                                                 |                                                                                               
AGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCTGCCAGCCTGGCAGAACTGCTGAAC  >  NC_000913/4189351‑4189543

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A