Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A16 F48 I0 R1 252 171.1 8397611 93.1% 7818175 99.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,189,448 C→T 100% Q1367* (CAG→TAG)  rpoC → RNA polymerase, beta prime subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,189,4480CT99.5% 614.0 / ‑5.2 181Q1367* (CAG→TAG) rpoCRNA polymerase, beta prime subunit
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (75/104);  total (77/104)
Fisher's exact test for biased strand distribution p-value = 4.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01

Only 100 of 187 total aligned reads displayed.

AGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCTGCCAGCCTGGCAGAACTGCTGAACG  >  NC_000913/4189351‑4189544
                                                                                                 |                                                                                                
 gcagcCGTGGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGAGCCCGGCAGGTACCGGTTACGCGTACCACTAgg                                                                                               <  2:41489/100‑1 (MQ=255)
  cagcCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGa                                                                                              >  2:1354810/1‑100 (MQ=255)
  cagcCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGa                                                                                              >  2:1755122/1‑100 (MQ=255)
  cagcCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGa                                                                                              >  1:1645953/1‑100 (MQ=255)
    gcCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCCGGTACCGGTTACCCGTCCCACTAGGacc                                                                                            >  2:3638164/1‑98 (MQ=255)
    gcCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATc                                                                                            <  2:1766853/100‑1 (MQ=255)
    gcCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATc                                                                                            <  2:2881532/100‑1 (MQ=255)
    gcCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGCGGGTCGTCTAATCCCAGCAGGTACAGGTTACGCGTACCACTAGGATc                                                                                            >  2:2832953/1‑100 (MQ=255)
     ccGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCg                                                                                           <  1:435916/100‑1 (MQ=255)
     ccGTTGCGAGCAAACGCGACGAACTGCGCGACCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGTTACCGGTTACTCGTACCACTAgtttcg                                                                                           <  2:2309510/100‑6 (MQ=255)
       gTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGCTTACGCGTACCACTAGGATCGTa                                                                                         >  2:1053692/1‑100 (MQ=255)
        ttGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTAt                                                                                        <  2:1818900/100‑1 (MQ=255)
        ttGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTAt                                                                                        <  1:2240428/100‑1 (MQ=255)
         tGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATg                                                                                       <  1:3677021/100‑1 (MQ=255)
           cGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCg                                                                                     >  2:1135775/1‑100 (MQ=255)
            gggCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGt                                                                                    >  1:2693609/1‑100 (MQ=255)
            gggCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGt                                                                                    >  1:2871757/1‑100 (MQ=255)
             ggCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTc                                                                                   >  1:3909924/1‑100 (MQ=255)
              gCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCg                                                                                  >  1:3615722/1‑100 (MQ=255)
               cAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGc                                                                                 <  1:3054635/100‑1 (MQ=255)
                aaaCGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGcc                                                                                <  2:10389/100‑1 (MQ=255)
                 aaCGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCg                                                                               <  2:3391343/100‑1 (MQ=255)
                  aCGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGt                                                                              <  1:637391/100‑1 (MQ=255)
                  aCGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGt                                                                              >  1:420967/1‑100 (MQ=255)
                    gcgACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGtgc                                                                            <  1:3170129/100‑1 (MQ=255)
                    gcgACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGtgc                                                                            <  1:985940/100‑1 (MQ=255)
                    gcgACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGtgc                                                                            <  1:1029863/100‑1 (MQ=255)
                    gcgACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGtgc                                                                            <  2:3539847/100‑1 (MQ=255)
                      gacgaACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGtgctg                                                                          >  2:67468/1‑100 (MQ=255)
                      gacgaACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGtgctg                                                                          <  2:3872815/100‑1 (MQ=255)
                        cgaACTGCGCGGCCTTAAAGAGAACGTTATCGTTGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCg                                                                        <  2:439519/100‑1 (MQ=255)
                         gaACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTTATCCCGGCAGGTACCTGTTACGCGTACCACTAGGATTGTATGCGTCGCCGTGCTGCg                                                                        <  2:398399/99‑1 (MQ=255)
                         gaACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCgg                                                                       <  1:1079001/100‑1 (MQ=255)
                          agcTGCGCGGCCTGAAGGAGGACGTTGTCGTGGGTCGTGTGATCCCGGCAGGTACCGGTTGCGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCggg                                                                      <  2:3342170/98‑1 (MQ=255)
                          aaCTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCggg                                                                      <  1:1541033/100‑1 (MQ=255)
                           aCTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGt                                                                     >  1:926738/1‑100 (MQ=255)
                            cTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCAGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTg                                                                    <  1:3841127/100‑1 (MQ=255)
                            cTGCACGGCATGAAAGAGAACGTTATCGTGGGACGACTGAACCCGACAGGTACCGGTTACGCGTGCACCAAGGACCGTATGCGCTGCCGCGCTTCggg                                                                      >  2:797330/1‑98 (MQ=255)
                             tGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGa                                                                   >  2:3596856/1‑100 (MQ=255)
                              gcgcGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACGGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGaa                                                                  >  1:3532393/1‑100 (MQ=255)
                              gcgcGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGaa                                                                  <  1:3368284/100‑1 (MQ=255)
                                gcgGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGc                                                                >  2:3217337/1‑100 (MQ=255)
                                    ccTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTc                                                              >  2:2087114/1‑98 (MQ=255)
                                    ccTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCg                                                            <  1:2914497/100‑1 (MQ=255)
                                      ttaaaGAGAACGTTATCGTGGGGCGTCTTATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGc                                                          <  1:3923531/98‑1 (MQ=255)
                                      tGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGc                                                          >  1:2153375/1‑100 (MQ=255)
                                      tGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGc                                                          <  1:2994157/100‑1 (MQ=255)
                                          agagAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCa                                                      <  2:55514/100‑1 (MQ=255)
                                          agagAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCa                                                      <  2:1232786/100‑1 (MQ=255)
                                           gggaACGTTATCGTGGGTCGTCTGATCGCTGCAGGGACCGGTTAGGCGTAGCACTAGGATCGTATGCGTCGCCGTTCTGCGGGTGAAGCTCCGGCTGCAc                                                     <  2:1877653/98‑1 (MQ=255)
                                             gaACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCg                                                   <  2:42857/100‑1 (MQ=255)
                                             gaACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCg                                                   >  1:1410077/1‑100 (MQ=255)
                                               aCGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCa                                                 >  2:2337994/1‑100 (MQ=255)
                                                 gTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAgg                                               <  1:2386598/100‑1 (MQ=255)
                                                 gTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAgg                                               >  1:3650743/1‑100 (MQ=255)
                                                  ttATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGt                                              <  1:288868/100‑1 (MQ=255)
                                                   tATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTg                                             <  2:2153372/100‑1 (MQ=255)
                                                    aTCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGa                                            >  1:3805796/1‑100 (MQ=255)
                                                     tCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGAc                                           <  1:3259869/100‑1 (MQ=255)
                                                      cGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACt                                          <  1:3186010/100‑1 (MQ=255)
                                                        tGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGc                                        <  2:3238578/100‑1 (MQ=255)
                                                        tGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGc                                        >  1:1136209/1‑100 (MQ=255)
                                                         gggTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCa                                       <  2:3779566/100‑1 (MQ=255)
                                                         gggTCGTCTGATCCCGGCAGGGACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCa                                       <  1:2447717/100‑1 (MQ=255)
                                                          gatcttcTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCag                                      <  1:335190/95‑1 (MQ=255)
                                                           gtcgtcTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCaga                                     <  1:1338576/100‑1 (MQ=255)
                                                           gtcgtcTGATCCCGGCAGGTACCGGTTACGCGTAACACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCaga                                     >  2:1305036/1‑100 (MQ=255)
                                                            tcgtcTGACCTCGGCAGGTACCGCTTACGCGTACCACTAGGACCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCagaa                                    <  2:3926173/100‑1 (MQ=255)
                                                              gtcTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCagaaga                                  >  1:1440323/1‑100 (MQ=255)
                                                                cTGATCCCGGCAGGTACCGGTTACGCGTACGACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGAACCGCAGGTGACTGCAGAAGAc                                 <  2:1673180/99‑1 (MQ=255)
                                                                cTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACg                                >  2:2409373/1‑100 (MQ=255)
                                                                 tGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGc                               >  2:150352/1‑100 (MQ=255)
                                                                    tCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATc                            <  1:275198/100‑1 (MQ=255)
                                                                    tCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATc                            >  1:715182/1‑100 (MQ=255)
                                                                     cccGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCt                           >  2:4074530/1‑100 (MQ=255)
                                                                     cccGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCt                           >  2:880486/1‑100 (MQ=255)
                                                                     cccGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCt                           >  1:560941/1‑100 (MQ=255)
                                                                     cccGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAACACGc                               >  1:3981345/1‑96 (MQ=255)
                                                                      ccGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCtn                          <  1:3462341/100‑2 (MQ=255)
                                                                        ggCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCTGcc                        >  1:284023/1‑100 (MQ=255)
                                                                        ggCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCTGcc                        <  1:2578418/100‑1 (MQ=255)
                                                                          cAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCTGCCAg                      <  2:1017032/100‑1 (MQ=255)
                                                                           aGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCTGCCAGc                     <  1:3703018/100‑1 (MQ=255)
                                                                            ggTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCTGCCAGcc                    >  2:3055996/1‑100 (MQ=255)
                                                                             gTACCGGTTACGCGTACCACTAGGATCGTAAGCCTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCACCTGCCAGCCt                   >  2:2757372/1‑100 (MQ=255)
                                                                             gTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCTGCCAGcc                    >  2:91991/1‑99 (MQ=255)
                                                                              tACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCTGCCAGCCTg                  <  1:1685543/100‑1 (MQ=255)
                                                                               aCCGGTTACGCGTACAAGTAGGATCGCATGCGTGGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGGGACTGCAGAAGACGCATCTGCCAgn                     <  2:2490155/96‑2 (MQ=255)
                                                                                ccGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCTGCCAGCCTGGc                >  1:4111601/1‑100 (MQ=255)
                                                                                ccGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCTGCCAGCCTGGc                >  1:2612856/1‑100 (MQ=255)
                                                                                 cGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCTGCCAGCCTGGCa               >  2:2741706/1‑100 (MQ=255)
                                                                                 cGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCTGCCAGCCTGGCa               >  2:2995021/1‑100 (MQ=255)
                                                                                      aCGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCTGCCAGCCTGGCAGAAct          <  1:3879515/100‑1 (MQ=255)
                                                                                        gcgTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCAGCGGAGGTGACTGCAGAAGACGCATCTGCCAGCCTGGCAGAActgc        <  1:176088/100‑1 (MQ=255)
                                                                                         cgTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCTGCCAGCCTGGCAGAActgct       <  1:1954692/100‑1 (MQ=255)
                                                                                         cgTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCTGCCAGCCTGGCAGAActgct       <  2:2264813/100‑1 (MQ=255)
                                                                                          gTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCTGCCAGCCTGGCAGAActgctg      <  1:1468148/100‑1 (MQ=255)
                                                                                            accactAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCTGCCAGCCTGGCAGAACTGCTGaa    <  1:1280200/100‑1 (MQ=255)
                                                                                              cactaGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCTGCCAGCCTGGCAGAACTGCTGAAca  >  2:492079/1‑99 (MQ=255)
                                                                                              cactaGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCTGCCAGCCTGGCAGAACTGCTGAACg  >  2:3727918/1‑100 (MQ=255)
                                                                                                 |                                                                                                
AGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCTGCCAGCCTGGCAGAACTGCTGAACG  >  NC_000913/4189351‑4189544

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A