Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A16 F125 I1 R1 17 96.7 4814547 93.9% 4520859 99.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,810,759 G→A intergenic (+80/‑11) ygaH → / → mprA putative L‑valine exporter, norvaline resistance protein/transcriptional repressor of microcin B17 synthesis and multidrug efflux

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,810,7590GA98.2% 376.7 / ‑4.2 110intergenic (+80/‑11)ygaH/mprAputative L‑valine exporter, norvaline resistance protein/transcriptional repressor of microcin B17 synthesis and multidrug efflux
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base A (55/52);  total (57/53)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 110 total aligned reads displayed.

GTGATGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTC  >  NC_000913/2810662‑2810854
                                                                                                 |                                                                                               
gTGATGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATc                                                                                               <  2:2103913/100‑1 (MQ=255)
 tGATGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATc                                                                                               >  2:2118874/1‑99 (MQ=255)
 tGATGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCa                                                                                              <  2:764454/100‑1 (MQ=255)
  gATGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCAt                                                                                             >  2:578582/1‑100 (MQ=255)
  gATGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCAt                                                                                             >  1:995363/1‑100 (MQ=255)
    tGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATAc                                                                                           >  2:587414/1‑100 (MQ=255)
     ggCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGTCTGAAATTAATGAGATCATAcc                                                                                          <  2:1392779/100‑1 (MQ=255)
     ggCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATAcc                                                                                          >  2:1460940/1‑100 (MQ=255)
     ggCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATAcc                                                                                          <  1:1583230/100‑1 (MQ=255)
     ggCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATAc                                                                                           >  2:734056/1‑99 (MQ=255)
      gCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATAcc                                                                                          <  2:610428/99‑1 (MQ=255)
          ttATATAACCCACAAGAATCATTTTTTTTTTTTTTTTCATTTACTTTATTTGTCACTGTCTTTACTATATCGGCTGAAATTAATGAGATCATACCCAAAt                                                                                     >  2:2008227/1‑100 (MQ=255)
          ttATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAAt                                                                                     <  1:587413/100‑1 (MQ=255)
              ataACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGTCATACCCAAATGGAt                                                                                 <  1:492251/100‑1 (MQ=255)
               taACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATa                                                                                >  1:1568335/1‑100 (MQ=255)
                 aCCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGt                                                                              >  1:450927/1‑100 (MQ=255)
                  cccACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGtt                                                                             >  2:25106/1‑100 (MQ=255)
                   ccACAAGAATCATTTTTTTTTTTTTTTTCATTTACTTAATTTGTCACTATCGTTACTATTTCGGATGAAATTAATTAGATCATACCCAACTGGATACTTc                                                                            >  2:467344/1‑100 (MQ=255)
                   ccACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTc                                                                            >  1:427567/1‑100 (MQ=255)
                   ccACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTc                                                                            <  2:495800/100‑1 (MQ=255)
                    cacaAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATTAGGTCATACCCAAATGGATAGtttg                                                                           >  2:142297/1‑98 (MQ=255)
                    cacaAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCg                                                                           >  2:355323/1‑100 (MQ=255)
                     acaAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGt                                                                          <  1:1460939/100‑1 (MQ=255)
                        aGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTa                                                                       >  2:1677731/1‑100 (MQ=255)
                        aGAATCATTTTTCTAAAACAATACATTTACTTTATTTGGCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTa                                                                       >  2:1476338/1‑100 (MQ=255)
                          aaTCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACg                                                                     <  1:1739082/100‑1 (MQ=255)
                          aaTCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACg                                                                     >  1:1475417/1‑100 (MQ=255)
                           aTCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGc                                                                    >  1:1058109/1‑100 (MQ=255)
                              aTTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCa                                                                 >  2:568672/1‑100 (MQ=255)
                              aTTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCa                                                                 <  1:1104839/100‑1 (MQ=255)
                               tttttCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCAt                                                                <  2:1941374/100‑1 (MQ=255)
                               tttttCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCAt                                                                <  1:25106/100‑1 (MQ=255)
                                 tttCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTg                                                              <  2:259009/100‑1 (MQ=255)
                                 tttCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTg                                                              <  2:261278/100‑1 (MQ=255)
                                  ttCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGa                                                             <  2:450927/100‑1 (MQ=255)
                                    cTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGaa                                                            >  1:189863/1‑99 (MQ=255)
                                    cTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGaa                                                            >  2:1924970/1‑99 (MQ=255)
                                    cTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAAc                                                           >  1:1345116/1‑100 (MQ=255)
                                    cTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAAc                                                           >  2:220437/1‑100 (MQ=255)
                                    cTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAAc                                                           <  2:2246567/100‑1 (MQ=255)
                                     tAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACa                                                          <  1:568671/100‑1 (MQ=255)
                                      aaaaCAATACATTTACTTTATTTTTCACTCTCGTTACTATATCGGCTTAAATTAATGAGATCATACCCAAATTGATAGTTCATTTACGCCCATTTAAc                                                           <  2:904306/98‑1 (MQ=255)
                                      aaaaCAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACag                                                         >  1:2246569/1‑99 (MQ=255)
                                      aaaaCAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACag                                                         >  1:2246327/1‑99 (MQ=255)
                                      aaaaCAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACaa                                                         >  2:1023330/1‑100 (MQ=255)
                                        aaCAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAAt                                                       <  1:1929679/100‑1 (MQ=255)
                                        aaCAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAAt                                                       <  1:285615/100‑1 (MQ=255)
                                         aCAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATg                                                      >  2:932639/1‑100 (MQ=255)
                                          cAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGc                                                     <  1:324864/100‑1 (MQ=255)
                                           aaTACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACATATGcg                                                    >  2:585110/1‑99 (MQ=255)
                                              aCATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTaaa                                                 <  1:981212/100‑1 (MQ=255)
                                                   tACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTc                                            >  2:447096/1‑100 (MQ=255)
                                                    aCTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTcg                                           <  1:2151837/100‑1 (MQ=255)
                                                    aCTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTcg                                           <  2:840896/100‑1 (MQ=255)
                                                     cTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTcgc                                          <  1:865051/100‑1 (MQ=255)
                                                     cTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTcgc                                          >  1:785363/1‑100 (MQ=255)
                                                     cTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTcgc                                          >  2:1486355/1‑100 (MQ=255)
                                                      tttATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTcgcg                                         <  2:366994/100‑1 (MQ=255)
                                                      tttATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTcgcg                                         <  2:367309/100‑1 (MQ=255)
                                                      tttATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTcgcg                                         >  2:932300/1‑100 (MQ=255)
                                                      tttATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTcgcg                                         >  1:1096903/1‑100 (MQ=255)
                                                      tttATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTcgc                                          >  2:933701/1‑99 (MQ=255)
                                                       ttATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCgcgc                                        >  1:2284725/1‑100 (MQ=255)
                                                           ttGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAgc                                    <  1:651073/100‑1 (MQ=255)
                                                            tGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAgcc                                   <  1:760887/100‑1 (MQ=255)
                                                              tCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAgccgc                                 <  2:2151465/100‑1 (MQ=255)
                                                              tCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAgccgc                                 >  2:2300654/1‑100 (MQ=255)
                                                               cACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAgccgcc                                <  1:1549243/100‑1 (MQ=255)
                                                                ctcttccgatcTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCa                               <  2:2021765/90‑1 (MQ=255)
                                                                  tGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACg                             <  1:1023328/100‑1 (MQ=255)
                                                                  tGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACg                             <  1:1105857/100‑1 (MQ=255)
                                                                    tCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGaa                           <  1:734055/100‑1 (MQ=255)
                                                                         aCTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGAttt                      >  2:783796/1‑100 (MQ=255)
                                                                            atatCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTcc                   <  1:110420/100‑1 (MQ=255)
                                                                            atatCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTcc                   <  1:578581/100‑1 (MQ=255)
                                                                             cgatctcTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCt                  <  1:1294652/94‑1 (MQ=255)
                                                                             cgatctcTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCt                  <  1:1291369/94‑1 (MQ=255)
                                                                              atCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCtt                 >  2:2221908/1‑100 (MQ=255)
                                                                              atCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCtt                 >  1:483854/1‑100 (MQ=255)
                                                                              atCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCtt                 >  1:114054/1‑100 (MQ=255)
                                                                                 ggCTGAAATTAATGAGATCATACCCAAATTGATAGTTCGCTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATc              <  2:306600/100‑1 (MQ=255)
                                                                                 ggCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATc              >  2:141509/1‑100 (MQ=255)
                                                                                 ggCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATc              <  1:279859/100‑1 (MQ=255)
                                                                                  gCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCa             <  1:483824/100‑1 (MQ=255)
                                                                                  gCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCa             >  1:25547/1‑100 (MQ=255)
                                                                                   cTGAAATTAATGCGATCATACCCAAATGGATAGTTCGATTACGCCCATTGAACACATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAg            >  2:522101/1‑100 (MQ=255)
                                                                                   cTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTcgtagatcg            >  2:1294653/1‑92 (MQ=255)
                                                                                   cTGAAATTAATGAGATCATACCCAAATGGATAGTTCGATTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTcgtagatcg            >  2:1291370/1‑92 (MQ=255)
                                                                                    tGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAgg           <  2:1749471/100‑1 (MQ=255)
                                                                                    tGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAgg           <  2:2159136/100‑1 (MQ=255)
                                                                                     gAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGga          <  1:1486354/100‑1 (MQ=255)
                                                                                       aaTTAATGCGCTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGgaga        >  2:903872/1‑100 (MQ=255)
                                                                                       aaTTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGgaga        >  1:450444/1‑100 (MQ=255)
                                                                                       aaTTAATGAGATCATACCCAAATGGATAGTTAGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGgag         >  2:656087/1‑99 (MQ=255)
                                                                                         ttAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATc      >  2:264814/1‑100 (MQ=255)
                                                                                         ttAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATc      <  1:910058/100‑1 (MQ=255)
                                                                                           aaTGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCt    <  1:2300656/100‑1 (MQ=255)
                                                                                           aaTGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCt    <  1:437709/100‑1 (MQ=255)
                                                                                            aTGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCtt   >  1:2175616/1‑100 (MQ=255)
                                                                                             tGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTc  <  2:114053/100‑1 (MQ=255)
                                                                                                 |                                                                                               
GTGATGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTC  >  NC_000913/2810662‑2810854

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A