Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A10 F112 I1 R1
|
17 |
70.3 |
3408026 |
96.6% |
3292153 |
99.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,352,826 |
G→A |
P67L (CCG→CTG) |
arcB ← |
aerobic respiration control sensor histidine protein kinase, cognate to two‑component response regulators ArcA and RssB |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,352,826 | 0 | G | A | 98.7%
| 248.4
/ ‑4.3
| 74 | P67L (CCG→CTG) | arcB | aerobic respiration control sensor histidine protein kinase, cognate to two‑component response regulators ArcA and RssB |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base A (41/32); total (41/33) |
| Fisher's exact test for biased strand distribution p-value = 4.46e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.36e-01 |
CTCGCGCATCTCCTCCAGTTTTTGCACCAGCCGTGACAGACGTTGTCGTGACTCCTCCAGTTGCTCGACGACCACCGATAGAAAGTAGACCGCCCACGGCGTAATCAGCAAACCAAAGAAGATAGAACGAATAACATCAATGCTTTCGACCTGACCATGCAGCACCATGGTTACCGCCATTTGTACCACAAT > NC_000913/3352729‑3352920
|
ctcgcgcATCTCCTCCAGTTTTTGCACCAGCCGTGACAGACGTTGTCGTGACTCCTCCAGTTGCTCGACGACCACCGATAGAAAGTAGACCGCCCACAGc > 2:1052595/1‑100 (MQ=255)
cgcgcATCTCCTCCAGTTTTTGCACCAGCCGTGACAGACGTTGTCGTGACTCCTCCAGTTGCTCGACGACCACCGATAGAAAGTAGACCGCCCACAGCGt < 1:680405/100‑1 (MQ=255)
gcgcATCTCCTCCAGTTTTTGCACCAGCCGTGACAGACGTTGTCGTGACTCCTCCAGTTGCTCGACGACCACCGATAGAAAGTAGACCGCCCACAGCGTa > 2:14197/1‑100 (MQ=255)
cgcATCTCCTCCAGTTTTTGCACCAGCCGTGACAGACGTTGTCGTGACTCCTCCAGTTGCTCGACGACCACCGATAGAAAGTAGACCGCCCACAGCGTaa > 2:662127/1‑100 (MQ=255)
ctcctcCAGTTTTTGCACCAGCCGTGACAGACGTTGTCGTGACTCCTCCAGTTGCTCGACGACCACCGATAGAAAGTAGACCGCCCACAGCGTAATCAGc < 2:1091952/100‑1 (MQ=255)
cctccAGTTTTTGCACCAGCCGTGACAGACGTTGTCGTGACTCCTCCAGTTGCTCGACGACCACCGATAGAAAGTAGACCGCCCACAGCGTAATCAGCaa > 2:921409/1‑100 (MQ=255)
cctccAGTTTTTGCACCAGCCGTGACAGACGTTGTCGTGACTCCTCCAGTTGCTCGACGACCACCGATAGAAAGTAGACCGCCCACAGCGTAATCAGCaa > 1:302452/1‑100 (MQ=255)
cctccAGTTTTTGCACCAGCCGTGACAGACGTTGTCGTGACTCCTCCAGTTGCTCGACGACCACCGATAGAAAGCCGACCGCCCACAGCGTAATCAGCaa > 1:1464587/1‑100 (MQ=255)
cctccAGTTTTTGCACCAGCCGTGACAGACGTTGTCGTGACTCCTCCAGTTGCTCGACGACCACCGACAGAAAGTAGACCGCCCACAGCGTAATCAGCaa > 2:419540/1‑100 (MQ=255)
ctccAGTTTTTGCACCAGCCGTGACAGACGTTGTCGTGACTCCTCCAGTTGCTCGACGACCACCGATAGAAAGTAGACCGCCCACAGCGTAATCAGCaaa > 1:1629005/1‑100 (MQ=255)
tccAGTTTTTGCACCAGCCGTGACAGACGTTGTCGTGACTCCTCCAGTTGCTCGACGACCACCGATAGAAAGTAGACCGCCCACAGCGTAATCAGCa < 1:1507610/97‑1 (MQ=255)
tccAGTTTTTGCACCAGCCGTGACAGACGTTGTCGTGACTCCTCCAGTTGCTCGACGACCACCGATAGAAAGTAGACCGCCCACAGCGTAATCAGCAAAc > 2:1574536/1‑100 (MQ=255)
cAGTTTTTGCACCAGCCGTGACAGACGTTGTCGTGACTCCTCCAGTTGCTCGACGACCACCGATAGAAAGTAGACCGCCCACGGCGTAATCAGCAAAcc < 1:121041/99‑1 (MQ=255)
cAGTTTTTGCACCAGCCGTGACAGACGTTGTCGTGACTCCTCCAGTTGCTCGACGACCACCGATAGAAAGTAGACCGCCCACAGCGTAATCAGCAAACCa > 1:1583890/1‑100 (MQ=255)
gTTTTTGCACCAGCCGTGACAGACGTTGTCGTGACTCCTCCAGTTGCTCGACGACCACCGATAGAAAGTAGACCGCCCACAGCGTAATCAGCAAACCaaa < 2:722191/100‑1 (MQ=255)
tttGCACCAGCCGTGACAGACGTTGTCGTGACTCCTCCAGTTGCTCGACGACCACCGATAGAAAGTAGACCGCCCACAGCGTAATCAGCAAACCAaagaa < 1:1491663/100‑1 (MQ=255)
ttGCACCAGCCGTGACAGACGTTGTCGTGACTCCTCCAGTTGCTCGACGACCACCGATAGAAAGTAGACCGCCCACAGCGTAATCAGCAAACCAaagaag < 1:308202/100‑1 (MQ=255)
ttGCACCAGCCGTGACAGACGTTGTCGTGACTCCTCCAGTTGCTCGACGACCACCGATAGAAAGTAGACCGCCCACAGCGTAATCAGCAAACCAaagaag > 2:715099/1‑100 (MQ=255)
tGCACCAGCCGTAACAGACGTTGTCGTGACTCCTCCAGTTGCTCGACGACCACCGATAGAAAGTAGACCGCCCACAGCGTAATCAGCAAACCAAagaaga > 2:1115425/1‑100 (MQ=255)
gCACCAGCCGTGACAGACGTTGTCGTGACTCCTCCAGTTGCTCGACGACCACCGATAGAAAGTAGACCGCCCACAGCGTAATCAGCAAACCAAAGAAGAt < 2:1239067/100‑1 (MQ=255)
cACCAGCCGTGACAGACGTTGTCGTGACTCCTCCAGTTGCTCGACGACCACCGATAGAAAGTAGACCGCCCACAGCGTAATCAGCAAACCAAAGAAGATa > 2:1494027/1‑100 (MQ=255)
ccAGCCGTGACAGACGTTGTCGTGACTCCTCCAGTTGCTCGACGCCCACCGATAGAAAGTAGACCCCCCACAGCGTAATCAGCAAACCAAAGAAGATa > 2:42364/1‑98 (MQ=255)
ccAGCCGTGACAGACGTTGTCGTGACTCCTCCAGTTGCTCGACGACCACCGATAGAAAGTAGACCGCCCACAGCGTAATCAGCAAACCAAAGAAGATa < 1:281227/98‑1 (MQ=255)
ccAGCCGTGACAGACGTTGTCGTGACTCCTCCAGTTGCTCGACGACCACCGATAGAAAGTAGACCGCCCACAGCGTAATCAGCAAACCAAAGAAGATAGa > 1:1360250/1‑100 (MQ=255)
ccAGCCGTGACAGACGTTGTCGTGACTCCTCCAGTTGCTCGACGACCACCGATAGAAAGTAGACCGCCCACAGCGTAATCAGCAAACCAAAGAAGATAGa > 1:1062093/1‑100 (MQ=255)
ccAGCCGTGACAGACGTTGTCGTGACGCCTCCAGTTGCTCGACGACCACCGATAGAAAGTAGACCGCCCACAGCGTAATCAGCAAACAAAAAAAGATAGa > 2:111998/1‑100 (MQ=255)
aGCCGTGACAGACGTTGTCGTGACTCCTCCAGTTGCTCGACGACCACCGATAGAAAGTAGACCGCCCACAGCGTAATCAGCAAACCAAAGAAGATAGAAc > 2:1208146/1‑100 (MQ=255)
cGTGACAGACGTTGTCGTGACTCCTCCAGTTGCTCGACGACCACCGATAGAAAGTAGACCGCCCACAGCGTAATCAGCAAACCAAAGAAGATAGAACGaa < 1:188300/100‑1 (MQ=255)
cGTGACAGACGTTGTCGTGACTCCTCCAGTTGCTCGACGACCACCGATAGAAAGTAGACCGCCCACAGCGTAATCAGCAAACCAAAGAAGATAGAACGaa > 2:975446/1‑100 (MQ=255)
gTGACAGACGTTGTCGTGACTCCTCCAGTTGCTCGACGACCACCGATAGAAAGTAGACCGCCCACAGCGTAATCAGCAAACCAAAGAAGATAGAACGAAt < 1:921407/100‑1 (MQ=255)
gTGACAGACGTTGTCGTGACTCCTCCAGTTGCTCGACGACCACCGATAGAAAGTAGACCGCCCACAGCGTAATCAGCAAACCAAAGAAGATAGAACGAAt < 2:972184/100‑1 (MQ=255)
gTGACAGACGTTGTCGTGACTCCTCCAGTTGCTCGACGACCACCGATAGAAAGTAGACCGCCCACAGCGTAATCAGCAAACCAAAGAAGATAGAACGAAt < 2:527137/100‑1 (MQ=255)
tGACAGACGTTGTCGTGACTCCTCCAGTTGCTCGACGACCACCGATAGAAAGTAGACCGCCCACAGCGTAATCAGCAAACCAAAGAAGATAGAACGAATa < 1:892778/100‑1 (MQ=255)
gACGTTGTCGTGACTCCTCCAGTTGCTCGACGACCACCGATAGAAAGTAGACCGCCCACAGCGTAATCAGCAAACCAAAGAAGATAGAACGAATAACATc < 1:14197/100‑1 (MQ=255)
cGTTGTCGTGACTCCTCCAGTTGCTCGACGACCACCGATAGAAAGTAGACCGCCCACAGCGTAATCAGCAAACCAAAGAAGATAGAACGAATAACATCaa > 2:891210/1‑100 (MQ=255)
cGTTGTCGTGACTCCTCCAGTTGCTCGACGACCACCGATAGAAAGTAGACCGCCCACAGCGTAATCAGCAAACCAAAGAAGATAGAACGAATAACATCaa < 1:1389696/100‑1 (MQ=255)
ttGTCGTGACTCCTCCAGTTGCTCGACGACCACCGATAGAAAGTAGACCGCCCACAGCGTAATCAGCAAACCAAAGAAGATAGAACGAATAACATCAATg < 1:547312/100‑1 (MQ=255)
gTGACTCCTCCAGTTGCTCGACGACCACCGATAGAAAGTAGACCGCCCACAGCGTAATCAGCAAACCAAAGAAGATAGAACGAATAACATCAATGCTTTc < 2:1109918/100‑1 (MQ=255)
tGACTCCTCCAGTTGCTCGACGACCACCGATAGAAAGTAGACCGCCCACAGCGTAATCAGCAAACCAAAGAAGATAGAACGAATAACATCAATGCTTTc > 1:1177267/1‑99 (MQ=255)
tGACTCCTCCAGTTGCTCGACGACCACCGATAGAAAGTAGACCGCCCACAGCGTAATCAGCAAACCAAAGAAGATAGAACGAATAACATCAATGCTTTCg < 2:157904/100‑1 (MQ=255)
gACTCCTCCAGTTGCTCGACGACCACCGATAGAAAGTAGACCGCCCACAGCGTAATCAGCAAACCAAAGAAGATAGAACGAATAACATCAATGCTTTCg < 2:283172/99‑1 (MQ=255)
aCTCCTCCAGTTGCTCGACGACCACCGATAGAAAGTAGACCGCCCACAGCGTAATCAGCAAACCAAAGAAGATAGAACGAATAACATCAATGCTTTCGAc < 1:1566610/100‑1 (MQ=255)
ctcctcCAGTTGCTCGACGACCACCGATAGAAAGTAGACCGCCCACAGCGTAATCAGCAAACCAAAGAAGATAGAACGAATAACATCAATGCTTTCGAc > 1:39750/1‑99 (MQ=255)
tcttttaGTTGCTCGATGACTAGCGATAGAAAGTAGTCTGTGCACAGAGTAATTAGCATATTAATGAAGATAGAACGTATAACATCAATGCTTTCg < 1:1491662/90‑1 (MQ=255)
cctccAGTTGCTCGACGACCACCAATAGAAAATAGACCGCCCACAGCGTAATAAGCAAACCAAAGAAGATAGAACGAAAAACATCAATACTTTCGa > 1:151428/1‑96 (MQ=255)
ctccAGTTGCTCGACGACCACCGATAGAAAGTAGACCGCCCACAGCGTAATCAGCAAACCAAAGAAGATAGAACGAATAACATCAATGCTTTCGACCTGa > 2:1035390/1‑100 (MQ=255)
ctccAGTTGCTCGACGACCACCGATAGAAAGTAGACCGCCCACAGCGTAATCAGCAAACCAAAGAAGATAGAACGAATAACATCAATGCTTTCGACCTGa > 2:911818/1‑100 (MQ=255)
ccAGTTGCTCGACGACCACCGATAGAAAGTAGACCGCCCACAGCGTAATCAGCAAACCAAAGAAGATAGAACGAATAACATCAATGCTTTCGACCTGAcc > 1:1257095/1‑100 (MQ=255)
ccAGTTGCTCGACGACCACCGATAGAAAGTAGACCGCCCACAGCGTAATCAGCAAACCAAAGAAGATAGAACGAATAACATCAATGCTTTCGACCTGAcc > 1:1058623/1‑100 (MQ=255)
ccAGTTGCTCGACGACCACCGATAGAAAGTAGACCGCCCACAGCGTAATCAGCAAACCAAAGAAGATAGAACGAATAACATCAATGCTTTCGACCTGAcc > 2:1105534/1‑100 (MQ=255)
aGTTGCTCGACGACCACCGATAGAAAGTAGACCGCCCACAGCGTAATCAGCAAACCAAAGAAGATAGAACGAATCACATCAATGCTTTCCACCTGACCAt > 1:362025/1‑100 (MQ=255)
aGTTGCTCGACGACCACCGATAGAAAGTAGACCGCCCACAGCGTAATCAGCAAACCAAAGAAGATAGAACGAATAACATCAATGCTCTCGACCTGACCa > 1:1396784/1‑99 (MQ=255)
gTTGCTCGACGACCACCGATAGAAAGTAGACCGCCCACAGCGTAATCAGCAAACCAAAGAAGATAGAACGAATAACATCAATGCTTTCGACCTGACCATg < 1:111998/100‑1 (MQ=255)
gCTCGACGACCACCGATAGAAAGTAGACCGCCCACAGCGTAATCAGCAAACCAAAGAAGATAGAACGAATAACATCAATGCTTTCGACCTGACCATgcag > 2:75045/1‑100 (MQ=255)
gCTCGACGACCACCGATAGAAAGTAGACCGCCCACAGCGTAATCAGCAAACCAAAGAAGATAGAACGAATAACATCAATGCTTTCGACCTGACCATgcag < 1:1199921/100‑1 (MQ=255)
cTCGACGACCACCGATAGAAAGTAGACCGCCCACAGCGTAATCAGCAAACAAAAGAAGATAGAACGAATAACATCAATGCTTTCGACCTGACCATgcagc > 1:1087610/1‑100 (MQ=255)
cgacgaCCACCGATAGAAAGTAGACCGCCCACAGCGTAATCAGCAAACCAAAGAAGATAGAACGAATAACATCAATGCTTTCGACCTGACCATGCAGCAc < 2:1456880/100‑1 (MQ=255)
cgacgaCCACCGATAGAAAGTAGACCGCCCACAGCGTAATCAGCAAACCAAAGAAGATAGAACGAATAACATCAATGCTTTCGACCTGACCATGCAGCAc > 2:10567/1‑100 (MQ=255)
accaccGATAGAAAGTAGACCGCCCACAGCGTAATCAGCAAACCAAAGAAGATAGAACGAATAACATCAATGCTTTCGACCTGACCATGCAGCACCATgg < 1:1511861/100‑1 (MQ=255)
accaccGATAGAAAGTAGACCGCCCACAGCGTAATCAGCAAACCAAAGAAGATAGAACGAATAACATCAATGCTTTCGACCTGACCATGCAGCACCATgg < 1:10567/100‑1 (MQ=255)
accaccGATAGAAAGTAGACCGCCCACAGCGTAATCAGCAAACCAAAGAAGATAGAACGAATAACATCAATGCTTTCGACCTGACCATGCAGCACCATgg < 2:496549/100‑1 (MQ=255)
accaccGATAGAAAGTAGACCGCCCACAGCGTAATCAGCAAACCAAAGAAGATAGAACGAATAACATCAATGCTTTCGACCTGACCATGCAGCACCATgg < 2:302453/100‑1 (MQ=255)
ccGATAGAAAGTAGACCGCCCACAGCGTAATCAGCAAACCAAAGAAGATAGAACGAATAACATCAATGCTTTCGACCTGACCATGCAGCACCATGGTTAc > 1:371010/1‑100 (MQ=255)
gATAGAAAGTAGACCGCCCACAGCGTAATCAGCAAACCAAAGAAGATAGAACGAATAACATCAATGCTTTCGACCTGACCATGCAGCACCATGGTTACCg < 1:1671629/100‑1 (MQ=255)
aTAGAAAGTAGACCGCCCACAGCGTAATCAGCAAACCAAAGAAGATAGAACGAATAACATCAATGCTTTCGACCTGACCATGCAGCACCATGGTTACCGc > 1:1217942/1‑100 (MQ=255)
aTAGAAAGTAGACCGCCCACAGCGTAATCAGCAAACCAAAGAAGATAGAACGAATAACATCAATGCTTTCGACCTGACCATGCAGCACCATGGTTACCGc > 2:422301/1‑100 (MQ=255)
tAGAAAGTAGACCGCCCACAGCGTAATCAGCAAACCAAAGAAGATAGAACGAATAACATCAATGCTTTCGACCTGACCATGCAGCACCATGGTTACCGcc < 2:1062098/100‑1 (MQ=255)
gAAAGTAGACCGCCCACAGCGTAATCAGCAAACCAAAGAAGATAGAACGAATAACATCAATGCTTTCGACCTGACCATGCAGCACCATGGTTACCGCCAt > 2:843034/1‑100 (MQ=255)
gAAAGTAGACCGCCCACAGCGTAATCAGCAAACCAAAGAAGATAGAACGAATAACATCAATGCTTTCGACCTGACCATGCAGCACCATGGTTACCGCCAt > 1:781657/1‑100 (MQ=255)
tAGACCGCCCACAGCGTAATCAGCAAACCAAAGAAGATAGAACGAATAACATCAATGCTTTCGACCTGACCATGCAGCACCATGGTTACCGCCATTTGTa > 2:550092/1‑100 (MQ=255)
aGACCGCCCACAGCGTAATCAGCAAACCAAAGAAGATAGAACGAATAACATCAATGCTTTCGACCTGACCATGCAGCACCATGGTTACCGCCATTTGTAc < 1:959149/100‑1 (MQ=255)
ccGCCCACAGCGTAATCAGCAAACCAAAGAAGATAGAACGAATAACAGCAATGCTTTCGACCTGACCATGCAGCACCATGGTTACCGCCATTTGTACcac < 2:102220/100‑1 (MQ=255)
gCCCACAGCGTAATCAGCAAACCAAAGAAGATAGAACGAATAACATCAATGCTTTCGACCTGACCATGCAGCACCATGGTTACCGCCATTTGTACCACaa > 1:584960/1‑100 (MQ=255)
cccACAGCGTAATCAGCAAACCAAAGAAGATAGAACGAATAACATCAATGCTTTCGACCTGACCATGCAGCACCATGGTTACCGCCATTTGTACCACAAt > 1:1261979/1‑100 (MQ=255)
|
CTCGCGCATCTCCTCCAGTTTTTGCACCAGCCGTGACAGACGTTGTCGTGACTCCTCCAGTTGCTCGACGACCACCGATAGAAAGTAGACCGCCCACGGCGTAATCAGCAAACCAAAGAAGATAGAACGAATAACATCAATGCTTTCGACCTGACCATGCAGCACCATGGTTACCGCCATTTGTACCACAAT > NC_000913/3352729‑3352920
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
