Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F64 I1 R1 17 68.2 3417408 93.4% 3191859 100.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,541,791 G→A G462D (GGT→GAT)  feoB → ferrous iron transporter protein B and GTP‑binding protein; membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,541,7910GA96.8% 204.8 / ‑1.7 62G462D (GGT→GAT) feoBferrous iron transporter protein B and GTP‑binding protein; membrane protein
Reads supporting (aligned to +/- strand):  ref base G (2/0);  new base A (30/30);  total (32/30)
Fisher's exact test for biased strand distribution p-value = 4.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.78e-01

TGTCCTGCGGCGCGCGTCTGGCTATCTTCGCAGTATTCGCGGCTGCCTTCTTCGGGCAGAACGGTGCGCTGGCGGTCTTCTCGCTGTATATGCTGGGTATTGTGATGGCGGTGCTGACTGGCCTGATGCTCAAGTACACCATCATGCGCGGTGAAGCGACGCCGTTTGTCATGGAGTTGCCGGTCTATCATG  >  NC_000913/3541695‑3541886
                                                                                                |                                                                                               
tGTCCTGCGGCGCGCGTCTGGCTATCTTCGCAGTATTCGCGGCTGCCTTCTTCGGGCAGAACGGTGCGCTGGCGGTCTTCTCGCTGTATATGCTGGatat                                                                                              <  2:1867534/100‑1 (MQ=255)
   ccTGCGGCGCGCGTCTGGCTATCTTCGCAGTATTCGCGGCTGCCTTCTTCGGGCAGAACGGTGCGCTGGCGGTCTTCTCGCTGTATATGCTGGATATtgt                                                                                           <  2:929373/100‑1 (MQ=255)
   ccTGCGGCGCGCGTCTGGCTATCTTCGCAGTATTCGCGGCTGCCTTCTTCGGGCAGAACGGTGCGCTGGCGGTCTTCTCGCTGTATATGCTGGATATtgt                                                                                           <  2:2465211/100‑1 (MQ=255)
    cTGCGGCGCGCGTCTGGCTATCTTCGCAGTATTCGCGGCTGCCTTCTTCGGGCAGAACGGTGCGCTGGCGGTCTTCTCGCTGTATATGCTGGATATtgtg                                                                                          >  2:1147760/1‑100 (MQ=255)
    cTGCGGCGCGCGTCTGGCTATCTTCGCAGTATTCGCGGCTGCCTTCTTCGGGCAGAACGGTGCGCTGGCGGTCTTCTCGCTGTATATGCTGGATATtgtg                                                                                          >  1:756349/1‑100 (MQ=255)
    cTGCGGCGCGCGTCTGGCTATCTTCGCAGTATTCGCGGCTGCCTTCTTCGGGCAGAACGGTGCGCTGGCGGTCTTCTCGCTGTATATGCTGGATATtgtg                                                                                          >  2:756349/1‑100 (MQ=255)
         gcgcgcGTCTGGCTATCTTCGCAGTATTCGCGGCTGCCTTCTTCGGGCAGAACGGTGCGCTGGCGGTCTTCTCGCTGTATATGCTGGATATTGTGATggn                                                                                     <  2:1310699/100‑2 (MQ=255)
               ttccgatcttCTTCGCAGTATTCGCGGCTGCCTTCTTCGGGCAGAACGGTGCGCTGGCGGTCTTCTCGCTGTATATGCTGGATATTGTGATGGCGGTGCt                                                                               <  2:602188/91‑1 (MQ=255)
               ttccgatcttCTTCGCAGTATTCGCGGCTGCCTTCTTCGGGCAGAACGGTGCGCTGGCGGTCTTCTCGCTGTATATGCTGGATATTGTGATGGCGGTGCt                                                                               <  1:602188/91‑1 (MQ=255)
                  tGGCTATCTTCGCAGTATTCGCGGCTGCCTTCTTCGGGCAGAACGGTGCGCTGGCGGTCTTCTCGCTGTATATGCTGGATATTGTGATGGCGGTGCTGAc                                                                            <  2:2008542/100‑1 (MQ=255)
                   ggCTATCTTCGCAGTATTCGCGGCTGCCTTCTTCGGGCAGAACGGTGCGCTGGCGGTCTTCTCGCTGTATATGCTGGATATTGTGATGGCGGTGCTGACt                                                                           <  2:777478/100‑1 (MQ=255)
                   ggCTATCTTCGCAGTATTCGCGGCTGCCTTCTTCGGGCAGAACGGTGCGCTGGCGGTCTTCTCGCTGTATATGCTGGATATTGTGATGGCGGTGCTGACt                                                                           <  1:777478/100‑1 (MQ=255)
                    gCTATCTTCGCAGTATTCGCGGCTGCCTTCTTCGGGCAGAACGGTGCGCTGGCGGTCTTCTCGCTGTATATGCTGGATATTGTGATGGCGGTGCTGACTg                                                                          >  2:1131532/1‑100 (MQ=255)
                      tATCTTCGCAGTATTCGCGGCTGCCTTCTTCGGGCAGAACGGTGCGCTGGCGGTCTTCTCGCTGTATATGCTGGATATTGTGATGGCGGTGCTGACTGGc                                                                        >  2:1545492/1‑100 (MQ=255)
                      tATCTTCGCAGTATTCGCGGCTGCCTTCTTCGGGCAGAACGGTGCGCTGGCGGTCTTCTCGCTGTATATGCTGGATATTGTGATGGCGGTGCTGACTGGc                                                                        >  2:918907/1‑100 (MQ=255)
                       aTCTTCGCAGTATTCGCGGCTGCCTTCTTCGGGCAGAACGGTGCGCTGGCGGTCTTCTCGCTGTATATGCTGGATATTGTGATGGCGGTGCTGACTGGcc                                                                       <  2:1261295/100‑1 (MQ=255)
                            ctcAGTATTCGCGGCTGCCTTCTTCGGGCAGAACGGTGCGCTTGCGGTCTTCTCGCTGTATATGCTGGATATTGTGATGGCGGTGCTGACTGGCCTGATg                                                                  <  2:2173668/98‑1 (MQ=255)
                             gCAGTATTCGCGGCTGCCTTCTTCGGGCAGAACGGTGCGCTGGCGGTCTTCTCGCTGTATATGCTGGATATTGTGATGGCGGTGCTGACTGGCCTGAtac                                                                 <  2:1716256/100‑3 (MQ=255)
                             gCAGTATTCGCGGCTGCCTTCTTCGGGCAGAACGGTGCGCTGGCGGTCTTCTCGCTGTATATGCTGGATATTGTGATGGCGGTGCTGACTGGCCTGATGc                                                                 >  2:1365301/1‑100 (MQ=255)
                               aGTATTCGCGGCTGCCTTCTTCGGGCAGAACGGTGCGCTGGCGGTCTTCTCGCTGTATATGCTGGATATTGTGATGGCGGTGCTGACTGGCCTGATGCTc                                                               >  2:1941351/1‑100 (MQ=255)
                                   ttCGCGGCTGCCTTCTTCGGGCAGAACGGTGCGCTGGCGGTCTTCTCGCTGTATATGCTGGATATTGTGATGGCGGTGCTGACTGGCCTGATGCTCAAGt                                                           <  2:1618388/100‑1 (MQ=255)
                                     cgcgGCTGCCTTCTTCGGGCAGAACGGTGCGCTGGCGGTCTTCTCGCTGTATATGCTGGGTATTGTGATGGCGGTGCTGACTGGCCTGATGCTCAAGTac                                                         >  2:186424/1‑100 (MQ=255)
                                     cgcgGCTGCCTTCTTCGGGCAGAACGGTGCGCTGGCGGTCTTCTCGCTGTATATGCTGGGTATTGTGATGGCGGTGCTGACTGGCCTGATGCTCAAGTac                                                         >  1:186424/1‑100 (MQ=255)
                                     cgcgGCTGCCTTCTTCGGGCAGAACGGTGCGCTGGCGGTCTTCTCGCTGTATATGCTGGATATTGTGATGGCGGTGCTGACTGGCCTGATGCTCAAGTac                                                         >  2:1377701/1‑100 (MQ=255)
                                            gCCTTCTTCGGGCAGAACGGTGCGCTGGCGGTCTTCTCGCTGTATATGCTGGATATTGTGATGGCGGTGCTGACTGGCCTGATGCTCAAGTACACcatca                                                  <  2:2371882/100‑1 (MQ=255)
                                             ccTTCTTCGGGCAGAACGGTGCGCTGGCGGTCTTCTCGCTGTATATGCTGGATATTGTGATGGCGGTGCTGACTGGCCTGATGCTCAAGTACACcatcat                                                 >  2:459257/1‑100 (MQ=255)
                                             ccTTCTTCGGGCAGAACGGTGCGCTGGCGGTCTTCTCGCTGTATATGCTGGATATTGTGATGGCGGTGCTGACTGGCCTGATGCTCAAGTACACcatcat                                                 >  1:459257/1‑100 (MQ=255)
                                              cttcttCGGGCAGAACGGTGCGCTGGCGGTCTTCTCGCTGTATATGCTGGATATTGTGATGGCGGTGCTGACTGGCCTGATGCTCAAGTACACCATCATg                                                >  1:566967/1‑100 (MQ=255)
                                              cttcttCGGGCAGAACGGTGCGCTGGCGGTCTTCTCGCTGTATATGCTGGATATTGTGATGGCGGTGCTGACTGGCCTGATGCTCAAGTACACCATCATg                                                >  2:566967/1‑100 (MQ=255)
                                              cttcttCGGGCAGAACGGTGCGCTGGCGGTCTTCTCGCTGTATATGCTGGATATTGTGATGGCGGTGCTGACTGGCCTGATGCTCAAGTACACCATCATg                                                >  1:89386/1‑100 (MQ=255)
                                              cttcttCGGGCAGAACGGTGCGCTGGCGGTCTTCTCGCTGTATATGCTGGATATTGTGATGGCGGTGCTGACTGGCCTGATGCTCAAGTACACCATCATg                                                >  2:89386/1‑100 (MQ=255)
                                               ttcttcGGGCAGAACGGTGCGCTGGCGGTCTTCTCGCTGTATATGCTGGATATTGTGATGGCGGTGCTGACTGGCCTGATGCTCAAGTACACCATCATgc                                               <  2:1388286/100‑1 (MQ=255)
                                                    cGGGCAGAACGGTGCGCTGGCGGTCTTCTCGCTGTATATGCTGGATATTGTGATGGCGGTGCTGACTGGCCTGATGCTCAAGTACACCATCATGCGCGGt                                          <  2:680727/100‑1 (MQ=255)
                                                    cGGGCAGAACGGTGCGCTGGCGGTCTTCTCGCTGTATATGCTGGATATTGTGATGGCGGTGCTGACTGGCCTGATGCTCAAGTACACCATCATGCGCGGt                                          >  2:308804/1‑100 (MQ=255)
                                                    cGGGCAGAACGGTGCGCTGGCGGTCTTCTCGCTGTATATGCTGGATATTGTGATGGCGGTGCTGACTGGCCTGATGCTCAAGTACACCATCATGCGCGGt                                          >  1:308804/1‑100 (MQ=255)
                                                    cGGGCAGAACGGTGCGCTGGCGGTCTTCTCGCTGTATATGCTGGATATTGTGATGGCGGTGCTGACTGGCCTGATGCTCAAGTACACCATCATGCGCGGt                                          <  1:680727/100‑1 (MQ=255)
                                                      ggCAGAACGGTGCGCTGGCGGTCTTCTCGCTGTATATGCTGGATATTGTGATGGCGGTGCTGACTGGCCTGATGCTCAAGTACACCATCATGCGCGGTGa                                        >  2:1918688/1‑100 (MQ=255)
                                                         aGAACGGTGCGCTGGCGGTCTTCTCGCTGTATATGCTGGATATTGTGATGGCGGTGCTGACTGGCCTGATGCTCAAGTACACCATCATGCGCGGTGAAg                                      <  1:737244/99‑1 (MQ=255)
                                                         aGAACGGTGCGCTGGCGGTCTTCTCGCTGTATATGCTGGATATTGTGATGGCGGTGCTGACTGGCCTGATGCTCAAGTACACCATCATGCGCGGTGAAg                                      <  2:737244/99‑1 (MQ=255)
                                                          gAACGGTGCGCTGGCGGTCTTCTCGCTGTATATGCTGGATATTGTGATGGCGGTGCTGACTGGCCTGATGCTCAAGTACACCATCATGCGCGGTGAAGCg                                    <  1:192894/100‑1 (MQ=255)
                                                          gAACGGTGCGCTGGCGGTCTTCTCGCTGTATATGCTGGATATTGTGATGGCGGTGCTGACTGGCCTGATGCTCAAGTACACCATCATGCGCGGTGAAGCg                                    <  2:192894/100‑1 (MQ=255)
                                                          gAACGGTGCGCTGGCGGTCTTCTCGCTGTATATGCTGGATATTGTGATGGCGGTGCTGACTGGCCTGATGCTCAAGTACACCATCATGCGCGGTGAAGCg                                    >  2:1301681/1‑100 (MQ=255)
                                                                 gcgcTGGCGGTCTTCTCGCTGTATATGCTGGATATTGTGATGGCGGTGCTGACTGGCCTGATGCTCAAGTACACCATCATGCGCGGTGAAGCGACGCCGt                             >  2:1139732/1‑100 (MQ=255)
                                                                  cgcTGGCGGTCTTCTCGCTGTATATGCTGGATATTGTGATGGCGGTGCTGACTGGCCTGATGCTCAAGTACACCATCATGCGCGGTGAAGCGACGCCGtt                            >  2:1697627/1‑100 (MQ=255)
                                                                   gcTGGCGGTCTTCTCGCTGTATATGCTGGATATTGTGATGGCGGTGCTGACTGGCCTGATGCTCAAGTACACCATCATGCGCGGTGAAGCGACGCCGttt                           >  2:926792/1‑100 (MQ=255)
                                                                      ggCGGTCTTCTCGCTGTATATGCTGGATATTGTGATGGCGGTGCTGACTGGCCTGATGCTCAAGTACACCATCATGCGCGGTGAAGCGACGCCGTTTGTc                        >  2:1085772/1‑100 (MQ=255)
                                                                       gCGGTCTTCTCGCTGTATATGCTGGATATTGTGATGGCGGTGCTGACTGGCCTGATGCTCAAGTACACCATCATGCGCGGTGAAGCGACGCCGTTTGTCa                       <  2:2286104/100‑1 (MQ=255)
                                                                          gTCTTCTCGCTGTATATGCTGGATATTGTGATGGCGGTGCTGACTGGCCTGATGCTCAAGTACACCATCATGCGCGGTGAAGCGACGCCGTTTGTCATgg                    <  2:2547121/100‑1 (MQ=255)
                                                                              tctcGCTGTATATGCTGGATATTGTGATGGCGGTGCTGACTGGCCTGATGCTCAAGTACACCATCATGCGCGGTGAAGCGACGCCGTTTGTCATGGAGtt                >  2:2287609/1‑100 (MQ=255)
                                                                               ctcGCTGTATATGCTGGATATTGTGATGGCGGTGCTGACTGGCCTGATGCTCAAGTACACCATCATGCGCGGTGAAGCGACGCCGTTTGTCATGGAGTTg               <  2:947251/100‑1 (MQ=255)
                                                                                  gCTGTATATGCTGGATATTGTGATGGCGGTGCTGACTGGCCTGATGCTCAAGTACACCATCATGCGCGGTGAAGCGACGCCGTTTGTCATGGAGTTGCCg            <  2:928803/100‑1 (MQ=255)
                                                                                   cTGTATATGCTGGATATTGTGATGGCGGTGCTGACTGGCCTGATGCTCAAGTACACCATCATGCGCGGTGAAGCGACGCCGTTTGTCATGGAGTTGCCgg           <  2:785341/100‑1 (MQ=255)
                                                                                   cTGTATATGCTGGATATTGTGATGGCGGTGCTGACTGGCCTGATGCTCAAGTACACCATCATGCGCGGTGAAGCGACGCCGTTTGTCATGGAGTTGCCgg           <  1:785341/100‑1 (MQ=255)
                                                                                       atatGCTGGATATTGTGATGGCGGTGCTGACTGGCCTGATGCTCAAGTACACCATCATGCGCGGTGAAGCGACGCCGTTTGTCATGGAGTTGCCGGTCTa       >  2:698590/1‑100 (MQ=255)
                                                                                       atatGCTGGATATTGTGATGGCGGTGCTGACTGGCCTGATGCTCAAGTACACCATCATGCGCGGTGAAGCGACGCCGTTTGTCATGGAGTTGCCGGTCTa       >  1:698590/1‑100 (MQ=255)
                                                                                        tatGCTGGATATTGTGATGGCGGTGCTGACTGGCCTGATGCTCAAGTACACCATCATGCGCGGTGAAGCGACGCCGTTTGTCATGGAGTTGCCGGTCTAt      >  2:632488/1‑100 (MQ=255)
                                                                                        tatGCTGGATATTGTGATGGCGGTGCTGACTGGCCTGATGCTCAAGTACACCATCATGCGCGGTGAAGCGACGCCGTTTGTCATGGAGTTGCCGGTCTAt      >  1:632488/1‑100 (MQ=255)
                                                                                           gCTGGATATTGTGATGGCGGTGCTGACTGGCCTGATGCTCAAGTACACCATCATGCGCGGTGAAGCGACGCCGTTTGTCATGGAGTTGCCGGTCTATCAt   <  2:821070/100‑1 (MQ=255)
                                                                                           gCTGGATATTGTGATGGCGGTGCTGACTGGCCTGATGCTCAAGTACACCATCATGCGCGGTGAAGCGACGCCGTTTGTCATGGAGTTGCCGGTCTATCAt   >  2:2154236/1‑100 (MQ=255)
                                                                                           gCTGGATATTGTGATGGCGGTGCTGACTGGCCTGATGCTCAAGTACACCATCATGCGCGGTGAAGCGACGCCGTTTGTCATGGAGTTGCCGGTCTATCAt   <  1:821070/100‑1 (MQ=255)
                                                                                           gCTGGATATTGTGATGGCGGTGCTGACTGGCCTGATGCTCAAGTACACCATCATGCGCGGTGAAGCGACGCCGTTTGTCATGGAGTTGCCGGTCTATCAt   >  2:1638682/1‑100 (MQ=255)
                                                                                            cTGGATATTGTGATGGCGGTGCTGACTGGCCTGATGCTCAAGTACACCATCATGCGCGGTGAAGCGACGCCGTTTGTCATGGAGTTGCCGGTCTATCATg  <  2:644034/100‑1 (MQ=255)
                                                                                            cTGGATATTGTGATGGCGGTGCTGACTGGCCTGATGCTCAAGTACACCATCATGCGCGGTGAAGCGACGCCGTTTGTCATGGAGTTGCCGGTCTATCATg  <  1:644034/100‑1 (MQ=255)
                                                                                                |                                                                                               
TGTCCTGCGGCGCGCGTCTGGCTATCTTCGCAGTATTCGCGGCTGCCTTCTTCGGGCAGAACGGTGCGCTGGCGGTCTTCTCGCTGTATATGCTGGGTATTGTGATGGCGGTGCTGACTGGCCTGATGCTCAAGTACACCATCATGCGCGGTGAAGCGACGCCGTTTGTCATGGAGTTGCCGGTCTATCATG  >  NC_000913/3541695‑3541886

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A