Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A16 F201 I0 R1 306 176.9 8831342 93.4% 8248473 99.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,520,500 Δ1 bp 16.8% intergenic (‑176/+171) yjhU ← / ← yjhF putative DNA‑binding transcriptional regulator; KpLE2 phage‑like element/putative transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,520,5000A.16.8% 553.5 / 109.2 196intergenic (‑176/+171)yjhU/yjhFputative DNA‑binding transcriptional regulator; KpLE2 phage‑like element/putative transporter
Reads supporting (aligned to +/- strand):  ref base A (87/74);  new base . (18/15);  total (106/90)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.59e-01

Only 100 of 200 total aligned reads displayed.

TGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGC  >  NC_000913/4520401‑4520599
                                                                                                   |                                                                                                    
 ggCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGAc                                                                                                     <  1:787711/100‑1 (MQ=255)
 ggCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGAc                                                                                                     <  1:2148977/100‑1 (MQ=255)
 ggCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGAGATCGCCCGCTGTTCCCCCATACTGTAAAGCAGGAGAc                                                                                                     >  2:1916597/1‑100 (MQ=255)
   cATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACAt                                                                                                   >  2:1641228/1‑100 (MQ=255)
    aTGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACa                                                                                                    >  1:3430259/1‑98 (MQ=255)
    aTGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATg                                                                                                  >  1:2292267/1‑100 (MQ=255)
       aTTTTATACAGATATTTTATTCTTTTACATGAAATATATCAACATACTATTTTATTTTTCACCTGCTGTAACTCCATACTGTAAAGCCGGAGACATG‑CTc                                                                                              >  1:4215956/1‑100 (MQ=255)
       aTTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTc                                                                                              >  1:2779476/1‑100 (MQ=255)
       aTTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTc                                                                                              <  2:388279/100‑1 (MQ=255)
        ttttATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTcc                                                                                             >  2:3724547/1‑100 (MQ=255)
          ttATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTccag                                                                                           >  1:2676539/1‑98 (MQ=255)
           tataCAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGc                                                                                          >  2:1244976/1‑100 (MQ=255)
           tataCAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAG‑CATGTCTCCGGc                                                                                          <  1:500193/100‑1 (MQ=39)
            ataCAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTcc                                                                                             <  1:2928875/96‑1 (MQ=255)
            ataCAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCt                                                                                         <  1:4366440/100‑1 (MQ=255)
            ataCAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCt                                                                                         <  1:2084545/100‑1 (MQ=255)
            ataCAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAG‑CATGTCTCCGGCt                                                                                         <  2:1774289/100‑1 (MQ=38)
             taCAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGTTTTCGCCCGCTGATCCCCCATACTGTTTATCCGTAGACATG‑CTccccc                                                                                          >  1:2174741/1‑95 (MQ=255)
             taCAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCtt                                                                                        <  1:4089293/100‑1 (MQ=255)
             taCAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAG‑CATGTCTCCGGCtt                                                                                        <  2:4275933/100‑1 (MQ=38)
             taCAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAG‑CATGTCTCCGGCtt                                                                                        <  1:1324171/100‑1 (MQ=38)
              aCAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAG‑CATGTCTCCGGCttt                                                                                       <  2:1523233/100‑1 (MQ=38)
              aCAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAG‑CATGTCTCCGGCttt                                                                                       <  1:1008231/100‑1 (MQ=38)
              aCAAATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCttt                                                                                       <  1:1510112/100‑1 (MQ=255)
               cAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTa                                                                                      >  1:453122/1‑100 (MQ=255)
               cAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTa                                                                                      <  2:4069742/100‑1 (MQ=255)
                aGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTAc                                                                                     >  1:3328693/1‑100 (MQ=255)
                aGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAG‑CATGTCTCCGGCTTTAc                                                                                     <  1:1581240/100‑1 (MQ=38)
                aGATATTTTATTCTTTTACAGGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTAc                                                                                     <  1:167897/100‑1 (MQ=255)
                 gATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAG‑CATGTCTCCGGCTTTACa                                                                                    <  2:906893/100‑1 (MQ=38)
                 gATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAG‑CATGTCTCCGGCTTTACa                                                                                    <  1:2846384/100‑1 (MQ=38)
                   tatTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGt                                                                                  <  2:1404568/100‑1 (MQ=255)
                   tatTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGt                                                                                  <  2:2546845/100‑1 (MQ=255)
                    atTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGt                                                                                  >  2:4003481/1‑99 (MQ=255)
                     ttttATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTAt                                                                                >  2:1093380/1‑100 (MQ=255)
                        cgatCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTAc                                                                             <  1:130704/97‑1 (MQ=255)
                         aTTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACt                                                                            <  2:2286878/100‑1 (MQ=255)
                          ttCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTg                                                                           <  1:2824542/100‑1 (MQ=255)
                            cTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTgag                                                                         <  2:3788573/100‑1 (MQ=255)
                             ttttACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTgag                                                                         <  1:876720/99‑1 (MQ=255)
                             ttttACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTg                                                                           >  1:1847908/1‑97 (MQ=255)
                                 aCATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACATTATTACTGAGAATac                                                                    >  1:964776/1‑100 (MQ=255)
                                  cATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGCCATG‑CTCCGGCTTTACAGTATTACTGAGAATaca                                                                   >  1:890392/1‑100 (MQ=255)
                                   aTGAAATAAACCAGCATACCATTTGAGTGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATacac                                                                  <  1:861484/100‑1 (MQ=255)
                                   aTGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATacac                                                                  <  2:2779707/100‑1 (MQ=255)
                                    tGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACAcc                                                                 <  1:3962154/100‑1 (MQ=255)
                                    tGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACAcc                                                                 <  2:3856578/100‑1 (MQ=255)
                                    tGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACAcc                                                                 <  1:591614/100‑1 (MQ=255)
                                      aaaTAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCa                                                               >  1:4244142/1‑100 (MQ=255)
                                      aaaTAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATAAAACCa                                                               >  1:3707453/1‑100 (MQ=255)
                                           aaCCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAg                                                          <  2:857584/100‑1 (MQ=255)
                                           aaCCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAg                                                          >  2:2452003/1‑100 (MQ=255)
                                             ccAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCa                                                        >  2:2850938/1‑100 (MQ=255)
                                               agtgtACCATGTGAGCGGTCGACGGCTGTCCCCCCATACTGTAAAGCCCGACACATC‑GTCCGGCTTTCCAGTATTACTGAGAATACACCCAGCAAGCAt                                                       <  2:3080401/95‑1 (MQ=255)
                                                 cATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CACCGGCTCTAAAGTCTTACTGAGAATACACCCATCAAGCATaa                                                     >  1:1265095/1‑99 (MQ=255)
                                                  aTACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATc                                                   <  2:3150843/100‑1 (MQ=255)
                                                       aTTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAgg                                              <  2:578806/100‑1 (MQ=255)
                                                        tttGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGt                                             >  2:40305/1‑100 (MQ=255)
                                                          tgggCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTc                                           <  1:3036509/97‑1 (MQ=255)
                                                           gAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCa                                          <  2:3475146/100‑1 (MQ=255)
                                                            aGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCaa                                         >  2:4077832/1‑100 (MQ=255)
                                                             gCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAat                                        >  1:3338318/1‑100 (MQ=255)
                                                               gATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAatat                                      >  2:4382274/1‑100 (MQ=255)
                                                               gATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAatat                                      <  2:2751396/100‑1 (MQ=255)
                                                                aTCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATc                                     >  1:3457952/1‑100 (MQ=255)
                                                                  cGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGc                                   <  1:1414866/100‑1 (MQ=255)
                                                                  cGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGc                                   <  2:4308345/100‑1 (MQ=255)
                                                                      cGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCt                               >  2:830645/1‑100 (MQ=255)
                                                                         tGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCtttt                            <  2:1200757/100‑1 (MQ=255)
                                                                          gTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATACTCGAAGGTTCAATATCGCCCCttttt                           >  1:1229805/1‑100 (MQ=255)
                                                                           ttCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAAGCGAAGGTTCAATATCGCCCCttgt                            >  2:200553/1‑96 (MQ=255)
                                                                            tCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGa                         <  1:1133936/100‑1 (MQ=255)
                                                                            tCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGa                         >  2:1311754/1‑100 (MQ=255)
                                                                            tCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGa                         <  1:2273723/100‑1 (MQ=255)
                                                                             cccccATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGAt                        >  1:3075088/1‑100 (MQ=255)
                                                                             cccccATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGAt                        >  2:478583/1‑100 (MQ=255)
                                                                              ccccATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATc                       >  2:2093559/1‑100 (MQ=255)
                                                                               cccATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCg                      <  2:2676554/100‑1 (MQ=255)
                                                                               cccATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCg                      <  1:4004576/100‑1 (MQ=255)
                                                                               cccATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCg                      >  2:3385498/1‑100 (MQ=255)
                                                                                   tACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCTCCAg                  >  1:3238194/1‑100 (MQ=255)
                                                                                   tACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAg                  >  1:2593074/1‑100 (MQ=255)
                                                                                    aCTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGc                 <  2:4220811/100‑1 (MQ=255)
                                                                                      tGTAAAGCCGGAG‑CATGTCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGa               >  1:1723948/1‑100 (MQ=38)
                                                                                       gTAAAGCCGGAG‑CATGTCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGCAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGAt              >  2:431845/1‑100 (MQ=38)
                                                                                        tAAAGCCGGAGCCATG‑CTCCGGCTTTACAGTATTACTGATAATACACCCATCAAGCATAATCGAAGGTTCTATATCGCCCCTTTTTGATCGCCAGCg                <  1:2804666/97‑1 (MQ=255)
                                                                                        tAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGAtt             <  2:2468710/100‑1 (MQ=255)
                                                                                        tAAAGCCGGAG‑CATGTCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGAtt             >  1:4314395/1‑100 (MQ=38)
                                                                                         aaaGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTc            >  2:817107/1‑100 (MQ=255)
                                                                                         aaaGCCGGAG‑CATGTCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTc            >  2:411190/1‑100 (MQ=38)
                                                                                         aaaGCCGGAG‑CATGTCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTc            >  2:1621796/1‑100 (MQ=38)
                                                                                          aaGCCGGAGACATG‑CTCCGGCTTTACAGTAGTACTGAGAATACACCCATCCAGCATAATCGAAGGTTCGATATCGCCCCTTTTTGATCGCCAGCGATTCg           <  1:4120960/100‑1 (MQ=255)
                                                                                          aaGCCGGAG‑CATGTCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCg           >  2:3755875/1‑100 (MQ=38)
                                                                                          aaGCCGGAG‑CATGTCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCg           >  2:3549813/1‑100 (MQ=38)
                                                                                           aGCCGGAG‑CATGTCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGt          >  2:3295520/1‑100 (MQ=38)
                                                                                            gCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGGTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTc         >  2:3128337/1‑100 (MQ=255)
                                                                                              cGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCAc       >  2:3632189/1‑100 (MQ=255)
                                                                                                 agaCATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGt    <  1:2483035/100‑1 (MQ=255)
                                                                                                   aCATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGc  <  2:3979243/100‑1 (MQ=255)
                                                                                                   aCATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGc  <  2:150163/100‑1 (MQ=255)
                                                                                                   |                                                                                                    
TGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGC  >  NC_000913/4520401‑4520599

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A