Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A10 F210 I0 R1 188 159.1 7866933 93.3% 7339848 99.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 459,110 G→A 21.6% A75T (GCC→ACC)  lon → DNA‑binding ATP‑dependent protease La

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913459,1100GA21.6% 264.7 / 63.4 143A75T (GCC→ACC) lonDNA‑binding ATP‑dependent protease La
Reads supporting (aligned to +/- strand):  ref base G (50/60);  new base A (13/18);  total (63/80)
Fisher's exact test for biased strand distribution p-value = 8.39e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.72e-01

Only 100 of 146 total aligned reads displayed.

GCGGCGATGGACCATGATAAAAAAATTATGCTGGTCGCGCAGAAAGAAGCTTCAACGGATGAGCCGGGTGTAAACGATCTTTTCACCGTCGGGACCGTGGCCTCTATATTGCAGATGCTGAAACTGCCTGACGGCACCGTCAAAGTGCTGGTCGAGGGGTTACAGCGCGCGCGTATTTCTGCGCTCTCTGACAATGGCG  >  NC_000913/459011‑459209
                                                                                                   |                                                                                                   
 cggcgATGGACCATGATAAAAAAATTATGCTGGTCGCGCAGAAAGAAGCTTCAACGGATGAGCCGGGTGTAAACGATCTTTTCACCGTCGGGACCATGGc                                                                                                    >  2:1375582/1‑100 (MQ=255)
  ggcgATGGACCATGATAAAAAAATTATGCTGGTCGCGCAGAAAGAAGCTTCAACGGATGAGCCGGGTGTAAACGATCTTTTCACCGTCGGGACCGTGGcc                                                                                                   <  1:1171019/100‑1 (MQ=255)
  ggcgATGGACCATGATAAAAAAATTATGCTGGTCGCGCAGAAAGAAGCTTCAACGGATGAGCCGGGTGTAAACGATCTTTTCACCGTCGGGACCGTGGcc                                                                                                   <  2:3622781/100‑1 (MQ=255)
   gcgATGGACCATGATAAAAAAATTATGCTGGTCGCGCAGAAAGAAGCTTCAACGGATGAGCCGGGTGTAAACGATCTTTTCACCGTCGGGACCGTGGCct                                                                                                  >  2:2983844/1‑100 (MQ=255)
    cgATGGACCATGATAAAAAAATTATGCTGGTCGCGCAGAAAGAAGCTTCAACGGATGAGCCGGGTGTAAACGATCTTTTCACCGTCGGGACCGTGGCctc                                                                                                 <  2:2512581/100‑1 (MQ=255)
          aCCATGATAAAACAATTATGCTGGTCGCGCAGAAAGAAGCTTCAACGGATGAGCCGGGTGTAAACGATCTTTTCACCGTCGGGACCGTGGCCTCTATAtt                                                                                           >  1:2580934/1‑100 (MQ=255)
          aCCATGATAAAAAAATTATGCTTGTCGCGCAGAAAGAAGCTTCAACTGATGAGCCGGGTGTAAACGATCTTTTCACCGTCGGGACCGTGGCCTCTATAtt                                                                                           <  1:624352/100‑1 (MQ=255)
          aCCATGATAAAAAAATTATGCTGGTCGCTCAGAAAGAAGCTTCAACGGATGAGCCGGGTGTAAACGATCTTTTCACCGTCGGGACCGTGGCCTCTATAtt                                                                                           <  2:869731/100‑1 (MQ=255)
           ccATGATAAAAAAATTATGCTGGTCGCGCAGAAAGAAGCTTCAACGGATGAGCCGGGAGTAAACGATATTTTCACCGTAGGGACCGTTGCCGCTATAtt                                                                                           >  1:3548163/1‑99 (MQ=255)
            cATGATAAAAAAATTATGCTGGTCGCGCAGAAAGAAGCTTCAACGGATGAGCCGGGTGTAAACGATCTTTTCACCGTCGTGACCGTGGCCTCTATATTGc                                                                                         <  2:3911065/100‑1 (MQ=255)
            cATGATAAAAAAATTATGCTGGTCGCGCAGAAAGAAGCTTCAACGGATGAGCCGGGTGTAAACGATCTTTTCACCGTCGGGACCGTGGCCTCTATATTGc                                                                                         >  1:3558691/1‑100 (MQ=255)
            cATGATAAAAAAATTATGCTGGTCGCGCAGAAAGAAGCTTCAACGGATGAGCCGGGTGTAAACGATCTTTTCACCGTCGGGACCGTGGCCTCTATATTGc                                                                                         <  1:713479/100‑1 (MQ=255)
              tGATAAAAAAATTATGCTGGTCGCGCAGAAAGAAGCTTCAACGGATGAGCCGGGTGTAAACGATCTTTTCACCGTCGGGACCGTGGCCTCTATATTGCAg                                                                                       >  1:3566640/1‑100 (MQ=255)
               gATAAAAAAATTATGCTGGTCGCGCAGAACGAAGGTTCAGCGGTTGAGCCTGTGTTAAACGATCTTTTCACCGTCGTGATCTTTGCCTTTAGAGTGc                                                                                         <  2:760119/97‑1 (MQ=255)
                aTAAAAAAATTATGCTGGTCGCGCAGAAAGAAGCTTCAACGGATGAGCCGGGTGTAAACGATCTTTTCACCGTCGGGACCGTGGCCTCTATATTGCAGAt                                                                                     >  1:2271206/1‑100 (MQ=255)
                aTAAAAAAATTATGCTGGTCGCGCAGAAAGAAGCTTCAACGGATGAGCCGGGTGTAAACGATCTTTTCACCGTCGGGACCGTGGCCTCTATATTGCAGAt                                                                                     <  1:798151/100‑1 (MQ=255)
                aTAAAAAAATTATGCTGGTCGCGCAGAAAGAAGCTTCAACGGATGAGACGGGTGTAAACGATCTTTTCACCGTCGGGACCGTGGCCTCTATATTGCAg                                                                                       >  1:3915812/1‑98 (MQ=255)
                 tAAAAAAATTATGCTGGTCGCGCAGAAAGAAGCTTCAACGGATGAGCCGGGTGTAAACGATCTTTTCACCGTCGGGACCGTGGCCTCTATATTGCAGATg                                                                                    >  1:809897/1‑100 (MQ=255)
                 tAAAAAAATTATGCTGGTCGCGCAGAAAGAAGCTTCAACGGATGAGCCGGGTGTAAACGATCTTTTCACCGTCGGGACCGTGACCTCTATATTGCAGATg                                                                                    <  1:1704272/100‑1 (MQ=255)
                 tAAAAAAATTATGCTGGTCGCGCAGAAAGAAGCTTCAACGGATGAGCCGGGTGTAAACGATCTTTTCACCGTCGGGACCGTGACCTCTATATTGCAGATg                                                                                    >  2:1704257/1‑100 (MQ=255)
                  aaaaaaaTTATGCTGGTCGCGCAGAAAGAAGCTTCAACGGATGAGCCGGGTGTAAACGATCTTTTCACCGTCGGGACCGTGGCCTCTATATTGCAGATGc                                                                                   <  1:1531553/100‑1 (MQ=255)
                  aaaaaaaTTATGCTGGTCGCGCAGAAAGAAGCTTCAACGGATGAGCCGGGTGTAAACGATCTTTTCACCGTCGGGACCGTGGCCTCTATATTGCAGATGc                                                                                   >  2:2742077/1‑100 (MQ=255)
                  aaaaaaaTTATGCTGGTCGCGCAGAAAGAAGCTTCAACGGATGAGCCGGGTGTAAACGATCTTTTCACCGTCGGGACCGTGACCTCTATATTGCAGATGc                                                                                   <  1:3685812/100‑1 (MQ=255)
                   aaaaaaTTATGCTGGTCGCGCAGAAAGAAGCTTCAACGGATGAGCCGGGTGTAAACGATCTTTTCACCGTCGGGACCGTGGCCTCTATATTGCAGATGCt                                                                                  <  2:603500/100‑1 (MQ=255)
                   aaaaaaTTATGCTGGTCGCGCAGAAAGAAGCTTCAACGGATGAGCCGGGTGTAAACGATCTTTTCACCGTCGGGACCGTGGCCTCTATATTGCAGATGCt                                                                                  >  1:2938470/1‑100 (MQ=255)
                   aaaaaaTTATGCTGGTCGCGCAGAAAGAAGCTTCAACGGATGAGCCGGGTGTAAACGATCTTTTCACCGTCGGGACCGTGACCTCTATATTGCAGATGCt                                                                                  >  1:3103238/1‑100 (MQ=255)
                    aaaaaTTATGCTAGTCGCGCAGAAAGAAGCTTCAACTGATGAGCCGGGTCTAAACGATCTTTTCAACGTAGGGACCATAGCCTCTATATTGCAGAt                                                                                     >  1:653607/1‑96 (MQ=255)
                       aaTTATGCTGGTGGCGCAGAAAGAAGCTTCAACGGATGAGCCGGGTGTAAACGATCTTTTCACCGTCGGGACCGTGGCCTCTATATTGCAGATGCTGaa                                                                               <  2:1194206/99‑1 (MQ=255)
                       aaTTATGCTGGTCGCGCAGAAAGAAGCTTCAACGGATGAGCCGGGTGTAAACGATCTTTTCACCGTCGGGACCGTGGCCTCTATATTGCAGATGCTGaaa                                                                              <  2:1466632/100‑1 (MQ=255)
                              cTGGTCGCGCAGAAAGAAGCTTCAACGGATGAGCCGGGTGTAAACGATCTTTTCACCGTCGGGACCGTGGCCTCTATATTGCAGATGCTGAAACTGCCTg                                                                       >  1:2472758/1‑100 (MQ=255)
                              cTGGTCGCGCAGAAAGAAGCTTCAACGGATGAGCCGGGTGTAAACGATCTTTTCACCGTCGGGACCGTGACCTCTATATTGCAGATGCTGAAACTGCCTg                                                                       >  1:1970362/1‑100 (MQ=255)
                                gttCGTGCAGCAATAAGCTTCTACGGGTGAGGCGGGTCTAAACGATCTTTTCACCGTCGCGACCGTGGCCTCTATATTGCAGATGCTGAAACTATCTGAc                                                                     <  2:1927373/98‑1 (MQ=255)
                                ggTCGCGCAGAAAGAAGCTTCAACGGATGAGCCGGGTGTAAACGATCTTTTCACCGTCGGGACCGTGACCTCTATATTGCAGATGCTGAAACTGCCTGAc                                                                     >  2:2666850/1‑100 (MQ=255)
                                ggTCGCGCAGAAAGAAGCTTCAACGGATGAGCCGGGTGTAAACGATCTTTTCACCGTCGGAACCGTGGCCTCTATATTGCAGATGCTGAAACTGCCTGAc                                                                     >  1:1883617/1‑100 (MQ=255)
                                 gTCGCGCAGAAAGAAGCTTCAACGGATGAGCCGGGTGTAAACGATCTTTTCACCGTCGGGACCGTGACCTCTATATTGCAGATGCTGAAACTGCCTGACg                                                                    <  1:3085319/100‑1 (MQ=255)
                                  tCGCGCAGAACGAAGCTTCAACGGATGAGCCGGGTGTAAACGATCTTTTCACCGTCGGGACCGTGGACTATATATTGCAGATGCTGAAACTGCCTGACgg                                                                   <  2:2580910/100‑1 (MQ=255)
                                  tCGCGCAGAAAGAAGCTTCAACGGATGAGCCGGGTGTAAACGATCTTTTCACCGTCGGGACCGTGGCCTCTATATTGCAGATGCTGAAACTGCCTGACgg                                                                   >  2:411149/1‑100 (MQ=255)
                                   cgcgCAGAAAGAAGCTTCAACGGATGAGCCGGGTGTAAACGATCTTTTCACCGTCGGGACCGTGGCCTCTATATTGCAGATGCTGAAACTGCCTGACggt                                                                  >  1:1663939/1‑99 (MQ=255)
                                    gcgcAGAAAGAAGCTTCAACGGATGAGCCGGGTGTAAACGATCTTTTCACCGTCGGGACCGTGGCCTCTATATTGCAGATGCTGAAACTGCCTGACGGCa                                                                 >  2:1644160/1‑100 (MQ=255)
                                     cgcAGAGAGAAGCTTCAACGGATGAGCCGGGTGTAAACGATCTTTTCACCGTCGGGACCGTGGCCTCTATATTGCAGATGCTGAAACTGCCTGACGGCAc                                                                >  1:368656/1‑100 (MQ=255)
                                     cgcAGAAAGAAGCTTCAACGGATGAGCCGGGTGTAAACGATCTTTTCACCGTCGGGACCGTGGCCTCTATATTGCAGATGCTGAAACTGCCTGACGGCAc                                                                >  2:3349917/1‑100 (MQ=255)
                                     cgcAGAAAGAAGCTTCAACGGATGAGCCGGGTGTAAACGATCTTTTCACCGTCGGGACCGTGGCCTCTATATTGCAGATGCTGAAACTGCCTGACGGCAc                                                                >  2:2819562/1‑100 (MQ=255)
                                     cgcAGAAAGAAGCTTCAACGGATGAGCCGGGTGTAAACGATCTTTTCACCGTCGGGACCGTGGCCTCTATATTGCAGATGCTGAAACTGCCTGACGGCAc                                                                >  1:1533894/1‑100 (MQ=255)
                                      gcAGAAAGAAGTTTTAACGGATTATTCGTGTGTAAACGATTTTTTCACCGTCGGGACCGTGACCTCTATATTGCAGATGCTGAAACTGCCTGACGGCAcc                                                               <  1:144577/100‑1 (MQ=255)
                                      gcAGAAAGAAGCTTCAACGGATGAGCCGGGTGTAAACGATCTTTTCACCGTCGGGACCGTGGCCTCTATATTGCAGATGCTGAAACTGCCTGACGGCAcc                                                               >  1:2326391/1‑100 (MQ=255)
                                        agaaagaaGCTTCAACGGATGAGCCGGGTGTAAACGATCTTTTCACCGTCGGGACCGTGGCCTCTATATTGCAGATGCTGAAACTGCCTGACGGCACCg                                                              <  1:905593/99‑1 (MQ=255)
                                            agaagCTTCAACGGATGAGCCGGGTGTAAACGATCTTTTCACCGTCGGGACCGTGGCCTCTATATTGCAGATGCTGAAACTGCCTGACGGCACCGTCaaa                                                         >  1:3036887/1‑100 (MQ=255)
                                             gaagCTTCAACGGATGAGCCGGGTGTAAACGATCTTTTCACCGTCGGGACCGTGGCCTCTATATTGCAGATGCTGAAACTGCCTGACGGCACCGTCAAAg                                                        <  1:3235212/100‑1 (MQ=255)
                                               agCTTCAACGGATGAGCCGGGTGTAAACGATCTTTTCACCGTCGGGACCGTGACCTCTATATTGCAGATGCTGAAACTGCCTGACGGCACCGTCAAAGTg                                                      <  1:1850207/100‑1 (MQ=255)
                                               agCTTCAACGGATGAGCCGGGTGTAAACGATCTTTTCACCGTCGGGACCCTGGCCTCTATATTGCAGATGCTGAAACTGCCTGACGGCACCGTCAAAGTg                                                      >  1:1752716/1‑100 (MQ=255)
                                                gCTTCAACGGATGAGCCGGGTGTAAACGATCTTTTCACCGTCGGGACCGTGGCCTCTATATTGCAGATGCTGAAACTGCCTGACGGTGCCGTCaggcagt                                                     <  2:1597398/100‑7 (MQ=255)
                                                 cTTCAACGGATGAGCCGGGTGTAAACGATCTTTTCACCGTCGGGACCGTGGCCTCTATATTGCAGATGCTGAAACTGCCTGACGGCACCGTCAAAGTGCt                                                    <  2:3558652/100‑1 (MQ=255)
                                                 cTTCAACGGATGAGCCGGGTGTAAACGATCTTTTCACCGTCGGGACCGTGACCTCTATATTGCAGATGCTGAAACTGCCTGACGGCACCGTCAAAGTGCt                                                    <  2:1409795/100‑1 (MQ=255)
                                                  ttcttctgATGAGCCGTGTGTAATCGATCTTTTCACCGTCGGGACCGTGACATCTATATTGCAGATGCTGAAACTGCCTGACGGCACCGTCAAAGTGCTg                                                   <  1:2218731/93‑1 (MQ=255)
                                                   tCAACGGATGAGCCGGGTGTAAACGATCTTTTCACCGTCGGGACCGTGGCCTCTATATTGCAGATGCTGAAACTGCCTGACGGCACCGTCAAAGTGCTgg                                                  >  1:232648/1‑100 (MQ=255)
                                                   tCAACGGATGAGCCGGGTGTAAACGATCTTTTCACCGTCGGGACCGTGACCTCTATATTGCAGATGCTGAAACTGCCTGACGGCACCGTCAAAGTGCTgg                                                  <  1:1790712/100‑1 (MQ=255)
                                                    cAACGGATGAGCCGGGTGTAAACGATCTTTTCACCGTCGGGACCGTGGCCTCTATATTGCAGATGCTGAAACTGCCTGACGGCACCGTCAAAGTGCTgg                                                  >  2:2282609/1‑99 (MQ=255)
                                                    cAACGGATGAGCCGGGTGTAAACGATCTTTTCACCGTCGGGACCGTGGCCTCTATATTGCAGATGCTGAAACTGCCTGACGGCACCGTCAAAGTGCTGGt                                                 >  2:2261525/1‑100 (MQ=255)
                                                      aCGGATGAGCCGGGTGTAAACGATCTTTTCACCGTCGGGACCGTGGCCTCTATATTGCAGATGCTGAAACTGCCTGACGGCACCGTCAAAGTGCTGGTCg                                               >  1:1981110/1‑100 (MQ=255)
                                                       cGGATGAGCCGGGTGTAAACGATCTTTTCACCGTCGGGACCGTGACCTCTATATTGCAGATGCTGAAACTGCCTGACGGCACCGTCAAAGTGCTGGTc                                                >  2:371316/1‑98 (MQ=255)
                                                       cGGATGAGCCGGGTGTAAACGATCTTTTCACCGTCGGGACCGTGACCTCTATATTGCAGATGCTGAAACTGCCTGACGGCACCGTCAAAGTGCTGGTCGa                                              >  2:3799973/1‑100 (MQ=255)
                                                         gATGAGCCGGGTGTAAACGATCTTTTCACCGTCGGGACCGTGGCCTCTATATTGCAGATGCTGAAACTGCCTGACGGCACCGTCAAAGTGCTGGTCGAgg                                            <  2:1981092/100‑1 (MQ=255)
                                                         gATGAGCCGGGTGTAAACGATCTTTTCACCGTCGGGACCGTGACCTCTATATTGCAGATGCTGAAACTGCCTGACGGCACCGTCAAAGTGCTGGTCGAgg                                            <  2:2144120/100‑1 (MQ=255)
                                                         gATGAGCCGGGTGTAAACGATCTTTTCACCGTCGGGACCGTGACCTCTATATTGCAGATGCTGAAACTGCCTGACGGCACCGTCAAAGTGCTGGTCGAgg                                            <  1:2078525/100‑1 (MQ=255)
                                                         gATGAGCCGGGTGTAAACGATCTTTTCACCGTCGGGACCGTGACCTCTATATTGCAGATGCTGAAACTGCCTGACGGCACCGTCAAAGTGCTGGTCGAgg                                            <  1:3278565/100‑1 (MQ=255)
                                                          aTGAGCCGGGTGTAAACGATCTTTTCACCGTCGGGACCGTGGCCTCTATATTGCAGATGCTGAAACTGCCTGACGGCACCGTCAAAGTGCTGGTCGAggg                                           <  1:1986342/100‑1 (MQ=255)
                                                               ccGGGTGTAAACGATCTTTTCACCGTCGGGACCGTTGTCTCTATATTGCAGATGCTGAAACTGCCTGACGGCACCGTCAAAGTGCTGGTCGAGGGGTTAc                                      <  1:1981673/100‑1 (MQ=255)
                                                               ccGGGTGTAAACGATCTTTTCACCGTCGGGACCGTGGCCTCTATATTGCAGATGCTTAAACTGCCTGACGGCACCGTTAAAGTGCTGGTCGAGGGGTTAc                                      <  2:857591/100‑1 (MQ=255)
                                                               ccGGGTGTAAACGATCTTTTCACCGTCGGGACCGTGGCCTCTATATTGCAGATGCTGAAACTGCCTGACGGCACCGTCAAAGTGCTGGTCGAGGGGTTAc                                      >  1:1373638/1‑100 (MQ=255)
                                                                 gggTGTAAACGATCTTTTCACCGTCGGGACCGTGGCCTCTATATTGCAGATGCTGAAACTGCCTGACGGCACCGTCAAAGTGCTGGTCGAGGGGTTACAg                                    <  2:2963675/100‑1 (MQ=255)
                                                                 gggTGTAAACGATCTTTTCACCGTCGGGACCGTGGCCTCTATATTGCAGATGCTGAAACTGCCTGACGGCACCGTCAAAGTGCTGGTCGAGGGGTTACAg                                    >  2:1973954/1‑100 (MQ=255)
                                                                   gtgtAAACGATCTTTTCACCGTCGGGACCGTGGCCTCTATATTGCAGATGCTGAAACTGCCTGACGGCACCGTCAAAGTGCTGGTCGAGGGGTTACAgcg                                  <  1:2159748/100‑1 (MQ=255)
                                                                     gtAAACGATCTTTTCACCGTCGGGACCGTGGCCTCTATATTGCAGATGCTGAAACTGCCTGACGGCACCGTCAAAGTGCTGGTCGAGGGGTTACAgcgcg                                >  2:203441/1‑100 (MQ=255)
                                                                     gtAAACGATCTTTTCACCGTAGGGACCGTGGCCTCTATATTGCAGATGCTGAAACTGCCTGACGGCACCGTCAAAGTGCTGGTCGAGGGGTTACAg                                    <  2:2942755/96‑1 (MQ=255)
                                                                      tAAACGATCTTTTCACCGTCGGGACCGTGGCCTCTATATTGCAGATGCTGAAACTGCCTGACGGCACCGTCAAAGTGCTGGTCGAGGGGTTACAgcgcgc                               >  2:1291138/1‑100 (MQ=255)
                                                                      tAAACGATCTTTTCACCGTCGGGACCGTGGCCTCTATATTGCAGATGCTGAAACTGCCTGACGGCACCGTAAACGTGCTGGTCGAGGGGTTACAgcgcgc                               <  2:3275652/100‑1 (MQ=255)
                                                                       aaaCGATCTTTTCACCGTCGGGACCGTGGCCTCTATATTGCAGATGCTGAAACTGCCTGACGGCACCGTCAAAGTGCTGGTCGAGGGGTTACAgcgcgcg                              >  2:3074529/1‑100 (MQ=255)
                                                                       aaaCGATCTTTTCACCGTCGGGACCGTGGCCTCTATATTGCAGATGCTGAAACTGCCTGACGGCACCGTCAAAGTGCTGGTCGAGGGGTTACAgcgcgcg                              <  2:2842916/100‑1 (MQ=255)
                                                                         aCGATCTTTTCACCGTCGGGACCGTGGCCTCTATATTGCAGATGCTGAAACTGCCTGACGGCACCGTCAAAGTGCTGGTCGAGGGGTTACAGcgcgcgcg                            <  1:3807421/100‑1 (MQ=255)
                                                                          cGATCTTTTCACCGTCGGGACCGTGACCTCTATATTGCAGATGCTGAAACTGCCTGACGGCACCGTCAAAGTGCTGGTCGAGGGGTTACAGCGCGCGCGt                           >  1:191791/1‑100 (MQ=255)
                                                                           gATCTTTTCACCGTCGTTACCGTGGCCTCTATATTGCAGATTCTGAAACTGCCTGACGGCACCGTCAAAGTGCTGGTCGAGGGGTTACAgcgcgcgcn                            <  1:2384752/98‑2 (MQ=255)
                                                                           gATCTTTTCACCGTCGGAACCGTGGCCTCTATATTGCAGATGCTGAAACTGCCTGACGGCACCGTCAAAGTGCTGGTCGAGGGGTTACAGCGCGCGCGTa                          >  2:1902105/1‑100 (MQ=255)
                                                                                 ttCACCGTCGGGACTGTGTCCTCTATATTGCAGATGCTGAAACTGCCTGACGGCACCGTCAAAGTGCTGGTCGAGGGGTTACAGCGCGCGCGTATTTCTg                    <  1:1800874/100‑1 (MQ=255)
                                                                                 ttCACCGTCGGGACCGTGGCCTCTATTTTGCAGATGTTGAAACTGCCTGACGGCACCGTCAAAGTGCTGGTCGAGGGGTTACAGCGCGCGCGTATTTCTg                    <  1:1624337/100‑1 (MQ=255)
                                                                                  tCACCGTCGGGACCGTGGCCTCTATATTGCAGATGCTGAAACTGCCTGACGGCACCGTCAAAGTGCTGGTCGAGGGGTTACAGCGCGCGCGTATTTCTgc                   <  1:1135950/100‑1 (MQ=255)
                                                                                   cACCGTCGGGACCGTGGCCTCTATATTGCAGATGCTGAAACTGCCTGACGGCACCGTCAAAGTGCTGGTCGAGGGGTTACAGCGCGCGCGTATTTCTgcg                  <  1:1317755/100‑1 (MQ=255)
                                                                                    aCCGTCGGGACCGTGGCCTCTATATTGCAGATGCTGAAACTGCCTGACGGCACCGTCAAAGTGCTGGTCGAGGGGTTACAGCGCGCGCGTATTTCTgcgc                 >  2:3713774/1‑100 (MQ=255)
                                                                                      cGTCGGGACCGTGACCTCTATATTGCAGATGCTGAAACTGCCTGACGGCACCGTCAAAGTGCTGGTCGAGGGGTTACAGCGCGCGCGTATTTCTGCGctc               <  2:1626856/100‑1 (MQ=255)
                                                                                        tCGGGACCGTGGCCTCTATATTGCAGATGCTGAAACTGCCTGACGGCACCGTCAAAGTGCTGGTCGAGGGGTTACAGCGCGCGCGTATTTCTGCGctctc             <  1:812584/100‑1 (MQ=255)
                                                                                           ggACCGTGACCTCTATATTGCAGATGCTGAAACTGCCTGACGGCACCGTCAAAGTGCTGGTCGAGGGGTTACAGCGCGCGCGTATTTCTGCGCTCTCTGa          <  1:3800012/100‑1 (MQ=255)
                                                                                             aCCGTGACCTCTATATTGCAGATGCTGAAACTGCCTGACGGCACCGTCAAAGTGCTGGTCGAGGGGTTACAGCGCGCGCGTATTTCTGCGCTCTCTGACa        <  1:2666874/100‑1 (MQ=255)
                                                                                              ccGTGGCCTCTATATTGCAGATGCTGAAACTGCCTGACGGCACCGTCAAAGTGCTGGTCGAGGGGTTACAGCGCGCGCGTATTTCTGCGCTCTCTGACaa       >  1:3100729/1‑100 (MQ=255)
                                                                                               cGTGGCCTCTATATTGCAGATGCTGAAACTGCCTGACGGCACCGTCAAAGTGCTGGTCGAGGGGTTACAGCGCGCGCGTATTTCTGCGCTCTCTGACAAt      >  2:127481/1‑100 (MQ=255)
                                                                                                gTGGCCTCTATATTGCAGATGCTGAAACTGCCTGACGGCACCGTCAAAGTGCTGGTCGAGGGGTTTCAGCGCGCGCGTATTTCTGCGCTCTCTGACAAt      >  2:1751418/1‑99 (MQ=255)
                                                                                                gTGGCCTCTATATTGCAGATGCTGAAACTGCCTGACGGCACCGTCAAAGTGCTGGTCGAGGGGTTACAGCGCGCGCGTATTTCTGCGCTCTCTGACAATg     >  2:354650/1‑100 (MQ=255)
                                                                                                gTGGCCTCTATATTGCAGATGCTGAAACTGCCTGACGGCACCGTCAAAGTGCTGGTCGAGGGGTTACAGCGCGCGCGTATTTCTGCGCTCTCTGACAATg     <  2:1350649/100‑1 (MQ=255)
                                                                                                gTGACCTCTATATTGCAGATGCTGAAACTGCCTGACGGCACCGTCAAAGTGCTGGTCGAGGGGTTACAGCGCGCGCGTATTTCTGCGCTCTCTGACAATg     >  2:2210949/1‑100 (MQ=255)
                                                                                                 tGGCCTCTATATTGCAGATGCTGAAACTGCCTGACGGCACCGTCAAAGTGCTGGTCGAGGGGTTACAGCGCGCGCGTATTTCTGCGCTCTCTGACAATgg    <  2:470462/100‑1 (MQ=255)
                                                                                                 tGGCCTCTATATTGCAGATGCTGAAACTGCCTGACGGCACCGTCAAAGTGCTGGTCGAGGGGTTACAGCGCGCGCGTATTTCTGCGCTCTCTGACAATgg    <  1:412349/100‑1 (MQ=255)
                                                                                                   gCCTCTATATTGCAGATGCTGAAACTGCCTGACGGCACCGTCAAAGTGCTGGTCGAGGGGTTACAGCGCGCGCGTATTTCTGCGCTCTCTGACAATGGCg  <  1:585433/100‑1 (MQ=255)
                                                                                                   |                                                                                                   
GCGGCGATGGACCATGATAAAAAAATTATGCTGGTCGCGCAGAAAGAAGCTTCAACGGATGAGCCGGGTGTAAACGATCTTTTCACCGTCGGGACCGTGGCCTCTATATTGCAGATGCTGAAACTGCCTGACGGCACCGTCAAAGTGCTGGTCGAGGGGTTACAGCGCGCGCGTATTTCTGCGCTCTCTGACAATGGCG  >  NC_000913/459011‑459209

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A