Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A10 F210 I0 R1 188 159.1 7866933 93.3% 7339848 99.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,520,504:1 +T 17.5% intergenic (‑180/+167) yjhU ← / ← yjhF putative DNA‑binding transcriptional regulator; KpLE2 phage‑like element/putative transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,520,5041.T17.5% 525.3 / 125.6 171intergenic (‑180/+167)yjhU/yjhFputative DNA‑binding transcriptional regulator; KpLE2 phage‑like element/putative transporter
Reads supporting (aligned to +/- strand):  ref base . (73/68);  new base T (20/10);  total (93/78)
Fisher's exact test for biased strand distribution p-value = 1.61e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.10e-02

Only 100 of 178 total aligned reads displayed.

ATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGAC  >  NC_000913/4520405‑4520603
                                                                                                    |                                                                                                   
aTGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATg                                                                                                      <  2:3635218/100‑1 (MQ=255)
 tGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑c                                                                                                    <  2:1226511/100‑1 (MQ=255)
   aTTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGATCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGAAGACATC‑Ct                                                                                                   >  2:3480861/1‑99 (MQ=255)
   aTTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAACGATAAACCGCTATTCACCCATACTGTAAAGCCGGAGACATg                                                                                                      >  1:39578/1‑97 (MQ=255)
       tataCAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTTTTCCCCCATACTTTAAAGCCGGAGACATG‑CTCCGGc                                                                                              <  2:1274756/100‑1 (MQ=255)
       tataCAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGc                                                                                              <  1:2486038/100‑1 (MQ=255)
         taCAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCtt                                                                                            <  1:1445587/100‑1 (MQ=255)
         taCAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCtt                                                                                            <  1:187686/100‑1 (MQ=255)
         taCAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAG‑CATGTCTCCGGCtt                                                                                            <  1:1590187/100‑1 (MQ=38)
         taCAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAG‑CATGTCTCCGGCt                                                                                             <  1:1719914/99‑1 (MQ=38)
           cAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCTCCCGCTGTTCCCCCTTACTGTATATCCGGAGACATG‑CTCCGGCTTTa                                                                                          <  2:1398135/100‑1 (MQ=255)
           cAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTa                                                                                          <  2:490154/100‑1 (MQ=255)
           cAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTa                                                                                          >  1:2194938/1‑100 (MQ=255)
           cAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTa                                                                                          <  2:701013/100‑1 (MQ=255)
           cAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTa                                                                                          >  2:3028383/1‑100 (MQ=255)
           cAGATATTTTATTCTTTTACATGAAATAAACCAGCATACAATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAACCGGAGACATG‑CTCCGGCTTTa                                                                                          >  2:659004/1‑100 (MQ=255)
            aGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTAc                                                                                         >  2:25537/1‑100 (MQ=255)
            aGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCAATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTAc                                                                                         >  2:1731291/1‑100 (MQ=255)
            aGATATTTTATTCTTTTACATGAAATAAACCAGCATACAATTTGAGCGACCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTAc                                                                                         >  2:1345516/1‑100 (MQ=255)
             gATATTTTTGTCCTTTACATGAAATACACCAGCATACCATTTATGCGATCGCCCGCTGTTCCCCCATACTGTACAGCCGGAGACATG‑CTCCGGCTTTACa                                                                                        <  2:2621303/100‑1 (MQ=255)
             gATATTTTATTCTTTTACATTAAATAAACCAGCATATCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAG‑CATGTCTCCGGCttt                                                                                           <  1:1671561/97‑1 (MQ=37)
             gATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCGCCATACTGTAAAGCCGGAG‑CATGTCTCCGGCTTTAcn                                                                                        <  2:3453174/100‑2 (MQ=37)
             gATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACa                                                                                        >  2:3188298/1‑100 (MQ=255)
               tatTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAg                                                                                       >  1:2686948/1‑99 (MQ=255)
                atTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGt                                                                                      >  2:1996788/1‑99 (MQ=255)
                   ttATTCTTTTACATGAAATAAACCAGCATACCATTTGAGTGATCGCCTGCTGTTCCCCTATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTa                                                                                  <  1:1232036/100‑1 (MQ=255)
                    tATTCTTTTACATAAAATAAACCAGCATACCATTTGACCGATCGCCCGCTGTTCCCGCATACTGTAAAGCCGGAGACATG‑ATCCGGCTTTACAGCAtt                                                                                   >  2:3118516/1‑98 (MQ=255)
                        cTTTTACATGAAATAAACCTGCATACCATTAGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTAAAGTAtt                                                                                   >  2:1677529/1‑94 (MQ=255)
                        cTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTgag                                                                             >  2:386471/1‑100 (MQ=255)
                        cTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATA‑CTCCGGCTTTACAGTATTACTgag                                                                             >  1:3800056/1‑100 (MQ=255)
                        cTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTATAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTgag                                                                             >  1:236926/1‑100 (MQ=255)
                           ttAGATTAAATAAACCAGCATACCATTTGAGCGATTGCCCGCGGTTTCCCCATACTGTAAAGCCTGAGACATT‑CTCCGGCTTTACAGTATTACTGAGAAt                                                                          <  2:1039184/100‑1 (MQ=255)
                           ttACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAAt                                                                          <  2:3825333/100‑1 (MQ=255)
                             aCATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATac                                                                        <  1:3182749/100‑1 (MQ=255)
                              cATGAAATAAACCAGCATACCATTTGAGCGATCGTCCGCTGTTCTCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATaca                                                                       <  1:1005129/100‑1 (MQ=255)
                              cATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATaca                                                                       >  2:3649787/1‑100 (MQ=255)
                              cATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCACCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATaca                                                                       >  2:2295077/1‑100 (MQ=255)
                               aTGAAATAAACCAGCATACCATTTGTGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATacac                                                                      <  1:989233/100‑1 (MQ=255)
                               aTGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATacac                                                                      >  2:2734408/1‑100 (MQ=255)
                               aTGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATacac                                                                      <  1:1216808/100‑1 (MQ=255)
                                tGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGATACATG‑CTCCGGCTTTACAGTATTACTGAGAATACAcc                                                                     <  2:2075843/100‑1 (MQ=255)
                                tGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACAcc                                                                     >  1:1204399/1‑100 (MQ=255)
                                tGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACAcc                                                                     >  1:468938/1‑100 (MQ=255)
                                tGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGAAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACAcc                                                                     >  1:3517853/1‑100 (MQ=255)
                                tGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAACCAAAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACAcc                                                                     >  1:1289456/1‑100 (MQ=255)
                                  aaaTAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCa                                                                   >  2:2386959/1‑100 (MQ=255)
                                     tAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCa                                                                <  2:1917906/100‑1 (MQ=255)
                                     tAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCa                                                                <  2:3552733/100‑1 (MQ=255)
                                     tAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCa                                                                <  1:3287342/100‑1 (MQ=255)
                                       aaCCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAg                                                              <  1:3499876/100‑1 (MQ=255)
                                        aCCAGCATACCATTTGAGCGATCGCCCGCTTTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGc                                                             <  1:2953469/100‑1 (MQ=255)
                                           aGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATa                                                          >  1:3214442/1‑100 (MQ=255)
                                              aTACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATc                                                       <  2:1702050/100‑1 (MQ=255)
                                                 ccATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGaa                                                    >  1:2143463/1‑100 (MQ=255)
                                                  cATTTGCGCGATCGCCCGCTGTTACCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAg                                                   >  2:2946127/1‑100 (MQ=255)
                                                    tttGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCTGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGt                                                 <  2:1588231/100‑1 (MQ=255)
                                                    tttGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGt                                                 >  1:835894/1‑100 (MQ=255)
                                                      tGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTc                                               >  1:1882597/1‑100 (MQ=255)
                                                        aGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTAAGAATACACACATCAAGCATAATCGAAGGTTCaa                                             >  1:2563306/1‑100 (MQ=255)
                                                              cGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGc                                       <  2:3675593/100‑1 (MQ=255)
                                                              cGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGc                                       <  1:2708347/100‑1 (MQ=255)
                                                              cGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGc                                       <  1:1895667/100‑1 (MQ=255)
                                                                 ccGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGTTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGcc                                      <  1:58458/98‑1 (MQ=255)
                                                                 ccGCTGTTCCCCCATACTGTAAAGACGGAGACATG‑CTACGGCTTTACAGTATTACTGAGAATACAACCATTAAGCATAATAGAAGGTTCAATATAGccc                                     >  2:3100872/1‑99 (MQ=255)
                                                                     tGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCtttt                                <  2:3745091/100‑1 (MQ=255)
                                                                       ttCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTg                              <  1:3354582/100‑1 (MQ=255)
                                                                        tCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGa                             <  2:745812/100‑1 (MQ=255)
                                                                        tCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTAAGAATACAACCATCAAGCATAATCAAAGGTTCAATATCACCCCTTTTTGa                             >  1:32337/1‑100 (MQ=39)
                                                                         cccccATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGAt                            >  2:1501562/1‑100 (MQ=255)
                                                                         cccccATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGAt                            >  1:3853800/1‑100 (MQ=255)
                                                                         cagtaaTACTGTAAAGCCGGAG‑CATGTCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGAt                            >  1:2775455/6‑100 (MQ=25)
                                                                          ccccATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATc                           <  2:1044670/100‑1 (MQ=255)
                                                                           gtaaTACTGTAAAGCCGGAG‑CATGTCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATGGCCCCTTTTTGATCg                          >  1:3874144/4‑100 (MQ=25)
                                                                           cgatctcTGTAAAGCCGGAG‑CATGTCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCg                          <  2:3496191/94‑1 (MQ=37)
                                                                           cccATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCg                          <  1:2526630/100‑1 (MQ=255)
                                                                           cccATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCg                          >  1:2985902/1‑100 (MQ=255)
                                                                           cccATACTGTAAAGCCGGAAACATG‑CTCCGGATTTACAGTATTACTGAGAATAAACACATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATc                           >  2:1136182/1‑99 (MQ=255)
                                                                             cATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGcc                        <  1:383735/100‑1 (MQ=255)
                                                                             cATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACACATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGcc                        >  2:45054/1‑100 (MQ=255)
                                                                             cATACTGGACAGCCTGAGTCATG‑CTCAGGCGTTACAGTCTTTCTCAGAATACACCCTACAAGCATAATTGATTGTTCAATATCGCCCCTTTTCGGTCGc                         <  2:3419777/99‑1 (MQ=255)
                                                                               tACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAg                      >  2:596813/1‑100 (MQ=255)
                                                                                aCTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGc                     <  1:2412405/100‑1 (MQ=255)
                                                                                  tGTAAAGCCGGAG‑CATGTCTCCGGCTTTACAGTAGTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGa                   >  2:3900251/1‑100 (MQ=38)
                                                                                  tGTAAAGCCGAAG‑CATGTCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGa                   >  1:2094604/1‑100 (MQ=38)
                                                                                  ngTAAAGCCGGAG‑CATGTCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGACCCTATTTGATCGCCAGCGa                   >  1:3238214/2‑100 (MQ=37)
                                                                                   gTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACAACCATCAAGCATAATAGAAGGTTCAATATCGCCCCTTTTTGCTTGCCAGAGAt                  >  2:1745595/1‑100 (MQ=255)
                                                                                   gTAAAGCCGGAG‑CATGTCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGAGAt                  >  1:1834705/1‑100 (MQ=38)
                                                                                    tAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGAtt                 >  1:2896356/1‑100 (MQ=255)
                                                                                     aaaGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGACTACACACATCAAGCATAATCGAAGGTTCAATATAACCCCTTTTTGAAAGCCAgaaa                   >  1:873248/1‑94 (MQ=255)
                                                                                     aaaGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGAtt                 >  1:1641941/1‑99 (MQ=255)
                                                                                     aaaGCCGGAG‑CATGTCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTc                >  1:1141801/1‑100 (MQ=38)
                                                                                     aaaGCCGGAG‑CATGTCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTc                >  2:3067775/1‑100 (MQ=38)
                                                                                      aaGCCGGAG‑CATGTCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCg               >  1:3004937/1‑100 (MQ=38)
                                                                                      aaGCCGGAG‑CATGTCTCCGGCTTTACAGTATTAATGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTCATCCCCAGCGATTc                >  2:890110/1‑99 (MQ=37)
                                                                                          cGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCAc           >  1:148539/1‑100 (MQ=255)
                                                                                             agaCATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGt        <  1:2419955/100‑1 (MQ=255)
                                                                                             agaCATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGt        >  1:2046857/1‑100 (MQ=255)
                                                                                              gaCATG‑CTCCGTCTTTATATTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCg         <  1:3558809/98‑1 (MQ=255)
                                                                                                cATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCa     >  1:1944642/1‑100 (MQ=255)
                                                                                                   g‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGAc  >  1:863043/1‑100 (MQ=255)
                                                                                                    |                                                                                                   
ATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGAC  >  NC_000913/4520405‑4520603

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A