Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F244 I0 R1	270	114.4	5840133	93.3%	5448844	99.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,001,304:1	(G)_7→8	100%	coding (960/2259 nt)	xdhA →	xanthine dehydrogenase, molybdenum binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,001,297	1	.	G	77.7%	249.2 / 76.8	94	coding (953/2259 nt)	xdhA	xanthine dehydrogenase, molybdenum binding subunit
Reads supporting (aligned to +/- strand): ref base . (8/13); new base G (39/34); total (47/47)
Fisher's exact test for biased strand distribution p-value = 3.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.07e-01

Only 100 of 108 total aligned reads displayed.

AAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCT‑GC‑‑GGG‑GGG‑GAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTCCCCTCGGCTGGTGCGA  >  NC_000913/3001198‑3001389

aaaTGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCT‑Gc                                                                                                  <  1:1664649/100‑1 (MQ=255)

 aaTGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCT‑GC‑‑g                                                                                               >  2:912721/1‑100 (MQ=255)

   tGGGGGTGAACCGGGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACAGCGGCGGTTATGCATCTCACGGGCACTCCATCGCTTCT‑GC‑‑gg                                                                                              <  1:2425529/99‑1 (MQ=255)

   tGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTAACGGGCACTCCATCGCTTCT‑GC‑‑ggg                                                                                             >  1:2593740/1‑100 (MQ=255)

     ggCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCT‑GC‑‑ggg‑gg                                                                                          >  2:1799337/1‑100 (MQ=255)

      gCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCT‑GC‑‑ggg‑ggg                                                                                         <  2:2063998/100‑1 (MQ=255)

        gTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCT‑GC‑‑ggg‑ggg‑gg                                                                                      <  2:868746/100‑2 (MQ=255)

        gTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCT‑GC‑‑ggg‑ggg‑gg                                                                                      <  1:73352/100‑2 (MQ=255)

        gTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCACCGCTTCT‑GC‑‑ggg‑ggg‑gg                                                                                      <  1:653095/100‑2 (MQ=255)

         tGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATGTCACGGGCACTCCATCGCTTCT‑GC‑‑ggg‑ggg‑gga                                                                                     <  1:1899103/100‑3 (MQ=255)

          gAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCT‑GC‑‑ggg‑ggg‑ggaa                                                                                    <  2:2684637/100‑4 (MQ=255)

          gAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCT‑GC‑‑ggg‑ggg‑ggaa                                                                                    <  1:1001290/100‑4 (MQ=255)

            aCCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCT‑GC‑‑ggg‑ggg‑ggaata                                                                                  >  2:389250/1‑95 (MQ=255)

             ccGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGGGTTTATGCATCTCAAGGGCACTCGATCGCTTCT‑GCG‑GGG‑GGG‑GAATaa                                                                                  >  1:1190090/1‑100 (MQ=255)

              cgcgACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCT‑GC‑‑GGG‑GGG‑GCATaa                                                                                  >  1:79765/1‑98 (MQ=255)

              cgcgAAGGAACATCGAAAGGTTATAGTCTGCATGTTCTGTCTAACACCCGCGCGTATGGATCTAACGGGCACTCCACCGCTTCTGGG‑‑GGG‑GGG‑GAATaaa                                                                                 <  1:2163383/100‑1 (MQ=255)

               gcgACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCT‑GCG‑GGG‑GGG‑GAATAAAg                                                                                <  1:1784659/100‑1 (MQ=255)

                  aCGGAACATTGAAAGGTTCTAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCT‑GCG‑GGG‑GGG‑GAATAAAGTCg                                                                             <  1:2114084/100‑1 (MQ=255)

                  aCGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCT‑GCG‑GGG‑GGG‑GAATAAAGTCg                                                                             <  2:2177833/100‑1 (MQ=255)

                  aCGGAACATTGAAAGGTTATAGTCTGGATGTGCTGTCTAAGACCGGCGCTTATGCATCTCGCGGGAACTCCATCGCTTCT‑GCG‑GGG‑GGG‑GAATAAAGTCg                                                                             <  1:1053236/100‑1 (MQ=255)

                   cGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCT‑GCG‑GGG‑GGG‑GAATAAAGTCGc                                                                            >  2:20113/1‑100 (MQ=255)

                   cGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCT‑GCG‑GGG‑GGG‑GAATAAAGTCGc                                                                            <  2:2096509/100‑1 (MQ=255)

                       aCATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACCCCGGCGCTTGTGAATCTCACGGGCACTCCATCGCTTCT‑GCG‑GGG‑GGG‑GAATAAAGTCGCTTan                                                                        <  1:2379867/100‑2 (MQ=255)

                       aCATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCT‑GCG‑GGG‑GGG‑GAATAAAGTCGCTTAc                                                                        >  2:1565299/1‑100 (MQ=255)

                          ttGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCT‑GCG‑GGG‑GGG‑GAATAAAGTCGCTTACCtt                                                                     <  2:478672/100‑1 (MQ=255)

                          ttGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCT‑GCG‑GGG‑GGG‑GAATAAAGTCGCTTACCtt                                                                     >  2:1722854/1‑100 (MQ=255)

                          ttGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCT‑GC‑‑GGG‑GGG‑GAATAAAGTCGCTTACCttt                                                                    <  1:1532367/100‑1 (MQ=255)

                           tGAAAGGTTATAGTCTGGCTGTTCCGCGTAACACGGGCGCTTATGGATCTCACGGGTACTCCATCGCTTCT‑GCG‑GGG‑GGG‑GAATAAAGTCGCGTACCt                                                                      <  1:1428848/98‑1 (MQ=255)

                           tGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCT‑GCG‑GGG‑GGG‑GAATAAAGTCGCTTACCttt                                                                    >  2:1824733/1‑100 (MQ=255)

                            gAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCT‑GCG‑GGG‑GGG‑GAATAAAGTCGCTTACCttt                                                                    >  1:2127300/1‑99 (MQ=255)

                            gAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCT‑GCG‑GGG‑GGG‑GAATAAAGTCGCTTACCTTTa                                                                   >  2:1064392/1‑100 (MQ=255)

                            gAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCT‑GCG‑GGG‑GGG‑GAATAAAGTCGCTTACCTTTa                                                                   >  2:512920/1‑100 (MQ=255)

                              aaGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCT‑GCG‑GGG‑GGG‑GAATAAAGTCGCTTACCTTTATc                                                                 <  2:2398038/100‑1 (MQ=255)

                              aaGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCT‑GC‑‑GGG‑GGG‑GAATAAAGTCGCTTACCTTTATcc                                                                <  2:2369909/100‑1 (MQ=255)

                                ggTTATAGTCTGGATGTTCTGTTTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCT‑GCG‑GGG‑GGG‑GAATAAAGTCGCTTACCTTTATCCt                                                               >  2:2469873/1‑100 (MQ=255)

                                 gTTATAGTCTGGATGTTCTTTCTAACACCGGCGCTTATGCATCTCACGTGCACTCGATCGCTTCT‑GCG‑GGG‑GGG‑GAAGAAAGTCGCTTACCTTTATCGTc                                                              >  1:2043103/1‑100 (MQ=255)

                                  ttATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCT‑GC‑‑GGG‑GGG‑GAATAAAGTCGCTTACCTTTATCCTCGt                                                            <  2:433177/100‑1 (MQ=255)

                                     taGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCT‑GCG‑GGG‑GGG‑GAATAAAGTCGCTTACCTTTATCCTCGTtg                                                          >  2:917833/1‑100 (MQ=255)

                                      ttccgatctGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCT‑GCG‑GGG‑GGG‑GAATAAAGTCGCTTACCTTTATCCTCGTtgt                                                         <  2:2278358/92‑1 (MQ=255)

                                      aGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCT‑GCG‑GGG‑GGG‑GAATAAAGTCGCTTACCTTTATCCTCGTtgt                                                         <  1:389248/100‑1 (MQ=255)

                                       gTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCT‑GCG‑GGG‑GGG‑GAATAAAGTCGCTTACCTTTATCCTCGTtgt                                                         >  1:55214/1‑99 (MQ=255)

                                       gTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCT‑GC‑‑GGG‑GGG‑GAATAAAGTCGCTTACCTTTATCCTCGTTGTGc                                                       <  2:882265/100‑1 (MQ=255)

                                         cTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCT‑GCG‑GGG‑GGG‑GAATAAAGTCGCTTACCTTTATCCTCGTTGTGcc                                                      >  2:1642849/1‑100 (MQ=255)

                                          tGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCT‑GC‑‑GGG‑GGG‑GAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTa                                                    >  2:2675503/1‑100 (MQ=255)

                                           ggATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCTCTCCATCGCTTCT‑GCG‑GGG‑GGG‑GAATAAAGTCGCTTACCTTTATCCTCGTTGTGcc                                                      <  1:141909/98‑1 (MQ=255)

                                               gTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCT‑GCG‑GGG‑GGG‑GAATAAAGTCGCTTACCTTTATCCTCGTtgtagatcggaa                                                >  1:2278343/1‑91 (MQ=255)

                                               gTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCT‑GCG‑GGG‑GGG‑GAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCt                                                >  1:2129261/1‑100 (MQ=255)

                                               gTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCT‑GCG‑GGG‑GGG‑GAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCt                                                <  1:106720/100‑1 (MQ=255)

                                               gTTCTGTCCAACACCGGTGCTTATGCATCTCACGGGCACTCCATCGCTTCT‑GCG‑GGG‑GGG‑GAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGc                                                 <  1:1893655/99‑1 (MQ=255)

                                                 tCTGTCTAACCCCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCT‑GCG‑GGG‑GGG‑GAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTa                                              <  2:189654/100‑1 (MQ=255)

                                                 tCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCT‑GCG‑GGG‑GGG‑GAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTa                                              <  2:189874/100‑1 (MQ=255)

                                                 tCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCT‑GCG‑GGG‑GGG‑GAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTa                                              >  2:2868504/1‑100 (MQ=255)

                                                    gTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCT‑GC‑‑GGG‑GGG‑GAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGt                                          <  2:2074473/100‑1 (MQ=255)

                                                    gTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCGTCT‑GCGGGGG‑GGG‑GAATAAAGTCGCTTACCTTTCTCCTCTTTGTGCCTACGCTTCCa                                            >  1:2362135/1‑100 (MQ=255)

                                                     tctcACACCGGCGCTTATGCATCTCTCGGGCACTCCATCGCTTCT‑GCG‑GGG‑GGG‑GAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTAc                                             <  1:158087/97‑1 (MQ=255)

                                                      cTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCT‑GC‑‑GGG‑GGGCGAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGt                                          >  1:2408139/1‑99 (MQ=255)

                                                      cTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCT‑GC‑‑GGG‑GGG‑GAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTc                                        >  2:2027876/1‑100 (MQ=255)

                                                       tAACAGCGTCGCTTATGCATCTCCCGGGCACTCCATCGCTTCT‑GCG‑GGG‑GGG‑GAATAAGGTCGCTTACCGGTATCCTCGTTGTGCCTACGCTTACAGTTc                                        <  1:2051444/100‑1 (MQ=255)

                                                       naaCACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCT‑GCGGGGG‑GGG‑GAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGtt                                         >  1:2485078/2‑100 (MQ=255)

                                                        aaCACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCT‑GCG‑GGG‑GGG‑GAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCa                                       <  2:2003477/100‑1 (MQ=255)

                                                         acacCGGCGCTTATGCATCTCACGGTCACTCCATCGCTTCT‑GCG‑GGG‑GGG‑GAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCaa                                      <  1:213281/100‑1 (MQ=255)

                                                          cacCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCT‑GCGGGGG‑GGG‑GAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCaa                                      <  2:2202093/100‑1 (MQ=255)

                                                                cgcTTATGCATCTCACGGGCACTCCATCGCTTCT‑GC‑‑GGG‑GGGTGAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCt                                <  1:1425188/99‑1 (MQ=255)

                                                                cgcTTATGCATCTCACGGGCACTCCATCGCTTCT‑GC‑‑GGG‑GGG‑GAATAAAGTCGCTTACCTTTATCCCCGTTGTGCCTACGCTTACAGTTCAAAGACCTgn                              <  1:1914528/100‑2 (MQ=255)

                                                                  ctgaTGCATCTCACGTGCACTCCATCGCTTCT‑GC‑‑GGGTGGG‑GAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCt                             <  1:848936/97‑1 (MQ=255)

                                                                  cTTATGCATCTCACGGGCACTCCATCGCTTCT‑GCG‑GGG‑GGG‑GATTAAAGTCGCTTACCTTTATCCTCGTTGTTCCTACGCTTACAGTTCAAAGACCTGCt                             <  1:398664/100‑1 (MQ=255)

                                                                  cTTATGCATCTCACGGGCACTCCATCGCTTCT‑GCG‑GGG‑GGG‑GAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTCCAGTTCAAAGACCTGCt                             >  2:1937792/1‑100 (MQ=255)

                                                                  cTTATGCATCTCACGGGCACTCCATCGCTTCT‑GCG‑GGG‑GGG‑GAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCt                             >  1:231072/1‑100 (MQ=255)

                                                                  cTTATGCATCTCACGGGCACTCCATCGCTTCT‑GC‑‑GGG‑GGG‑GAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCta                            <  2:154143/100‑1 (MQ=255)

                                                                   ttATGCATCTCACGGGCACTCCATCGCTTCT‑GCGGGGG‑GGG‑GAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCt                             <  2:810781/100‑1 (MQ=255)

                                                                   ttATGCATCTCACGGGCACTCCATCGCTTCT‑GC‑‑GGG‑GGG‑GAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCtat                           >  2:1169019/1‑100 (MQ=255)

                                                                     aTGCATCTCACGGGCACTCCATCGCTTCT‑GCG‑GGG‑GGG‑GAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCtata                          <  2:206155/100‑1 (MQ=255)

                                                                     aTGCATCTCACGGGCACTCCATCGCTTCT‑GCG‑GGG‑GGG‑GAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCtata                          <  1:2135624/100‑1 (MQ=255)

                                                                      tGCATCTCACGGGCACTCCATCGCTTCT‑GCG‑GGG‑GGG‑GAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATAc                         >  1:2685403/1‑100 (MQ=255)

                                                                      tGCATCTCACGGGCACTCCATCGCTTCT‑GCG‑GGG‑GGG‑GAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATAc                         <  2:2685419/100‑1 (MQ=255)

                                                                        cATCTCACGGGCACTCCATCGCTTCT‑GCG‑GGG‑GGG‑GAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCa                       >  2:964788/1‑100 (MQ=255)

                                                                        cATCTCACGGGCACTCCATCGCTTCT‑GCG‑GGG‑GGG‑GAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCa                       <  2:2123372/100‑1 (MQ=255)

                                                                         aTCTCACGGGCACTCCATCGCTTCT‑GCG‑GGG‑GGG‑GAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCaa                      >  2:2453781/1‑100 (MQ=255)

                                                                         aTCTCACGGGCACTCCATCGCTTCT‑GCG‑GGG‑GGG‑GAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCaa                      >  2:2453822/1‑100 (MQ=255)

                                                                         aTCTCACGGGCACTCCATCGCTTCT‑GCG‑GGG‑GGG‑GAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCaa                      >  2:1544258/1‑100 (MQ=255)

                                                                          tctcACGGGCACTCCATCGCTTCT‑GCG‑GGG‑GGG‑GAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAAc                     >  1:2089891/1‑100 (MQ=255)

                                                                          tctcACGGGCACTCCATCGCTTCT‑GC‑‑GGG‑GGG‑GAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAAcc                    <  2:1041153/100‑1 (MQ=255)

                                                                          tctcACGGGCACTCCATCGCTTCT‑GC‑‑GGG‑GGG‑GAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAAcc                    >  2:1169824/1‑100 (MQ=255)

                                                                             cACGGGCACTCCATCGCTTCT‑GCG‑GGG‑GGG‑GCATAAAGTCGCTTACCTCTATCCCCGTTGTGGCTACGCTTCTCTTTCAAACACCTCTTATTCCAACATc                  >  1:2255448/1‑100 (MQ=255)

                                                                             cACCGGCACTCCATCGCTTCT‑GC‑‑GGG‑GGG‑GAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTcc                 <  1:164345/100‑1 (MQ=255)

                                                                               cGGGCACTCCATCGCTTCT‑GCG‑GGG‑GGG‑GAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTccc                >  1:1240166/1‑100 (MQ=255)

                                                                               cGGGCACTCCATCGCTTCT‑GCG‑GGG‑GGG‑GAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTccc                >  2:1752568/1‑100 (MQ=255)

                                                                                gggCACTCCATCGCTTCT‑GCG‑GGG‑GGG‑GAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTcccc               <  1:2280364/100‑1 (MQ=255)

                                                                                 ggCACTCCATCGCTTCT‑GCG‑GGG‑GGG‑GAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTCCCCt              >  2:1159384/1‑100 (MQ=255)

                                                                                   cACTCCATCGCTTCG‑GCG‑GGG‑GGG‑GAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTCCCCTCg            <  2:2307005/100‑1 (MQ=255)

                                                                                    aCTCCATCGCTTCT‑GCG‑GGG‑GGG‑GAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTCCCCTCgg           <  2:2646850/100‑1 (MQ=255)

                                                                                    aCTCCATCGCTTCT‑GCG‑GGG‑GGG‑GAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTCCCCTCgg           >  1:269345/1‑100 (MQ=255)

                                                                                     cTCCATCGCTTCT‑GC‑‑GGG‑GGG‑GAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTCCCCTCGGCt         >  1:659397/1‑100 (MQ=255)

                                                                                      tCCATCGCTTCT‑GCG‑GGG‑GGG‑GAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTCCCCTCGGCt         >  2:2722397/1‑100 (MQ=255)

                                                                                       ccATCGCTTCT‑GCG‑GGG‑GGG‑GAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTCCCCTCGGCTg        >  2:242949/1‑100 (MQ=255)

                                                                                        cATCGCTTCT‑GCG‑GGG‑GGG‑GAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTCCCCTCGGCTgg       <  1:283878/100‑1 (MQ=255)

                                                                                        cATCGCTTCT‑GCG‑GGG‑GGG‑GAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTCCCCTCGGCTgg       <  2:87688/100‑1 (MQ=255)

                                                                                         aTCGCTTCT‑GCG‑GGG‑GGG‑GAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTCCCCTCGGCTGGt      >  1:2278035/1‑100 (MQ=255)

                                                                                         aTCGCTTCT‑GCG‑GGG‑GGG‑GAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCGACGCTTACAGTTCGAAGACCTGCTATACCAACCTCCCCTCGGCTGGt      <  1:633581/100‑1 (MQ=255)

                                                                                             cTTCT‑GCG‑GGG‑GGG‑GAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTCCCCTCGGCTGGTGCGa  <  2:2317595/100‑1 (MQ=255)

AAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCT‑GC‑‑GGG‑GGG‑GAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTCCCCTCGGCTGGTGCGA  >  NC_000913/3001198‑3001389

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A