Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A8 F183 I0 R1 119 96.4 4778198 94.0% 4491506 99.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,705,970 G→A 20.6% intergenic (‑180/+128) dppB ← / ← dppA dipeptide/heme ABC transporter permease/dipeptide/heme ABC transporter periplasmic binding protein; dipeptide chemotaxis receptor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,705,9700GA20.6% 236.1 / 52.2 107intergenic (‑180/+128)dppB/dppAdipeptide/heme ABC transporter permease/dipeptide/heme ABC transporter periplasmic binding protein; dipeptide chemotaxis receptor
Reads supporting (aligned to +/- strand):  ref base G (35/50);  new base A (18/4);  total (53/54)
Fisher's exact test for biased strand distribution p-value = 7.35e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.12e-01

Only 100 of 115 total aligned reads displayed.

CAGGGAATGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GAAT‑TTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCC  >  NC_000913/3705871‑3706069
                                                                                                   |                                                                                                        
cAGGGAATGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATg                                                                                                          >  1:1637264/1‑100 (MQ=255)
   ggAATGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATgcgg                                                                                                       >  2:433167/1‑100 (MQ=255)
    gAATGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATgcggc                                                                                                      >  1:527612/1‑100 (MQ=255)
     gcataaacaaagcgcactttgtcaataatctgaaaccccggcacatggccggggcttCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATgcggcg                                                                                                     <  1:652168‑M2/43‑1 (MQ=255)
     aaTGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATgcggcg                                                                                                     <  1:2154949/100‑1 (MQ=255)
        gAAGTATTGCTGCCGGATGGCGCTGCACGCAGGGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTa                                                                                                  <  2:2049417/100‑1 (MQ=255)
         aaGTAGTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTaa                                                                                                 <  2:1462738/100‑1 (MQ=255)
          aGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAAc                                                                                                <  2:2163682/100‑1 (MQ=255)
           gTATTGCTGCCGGATGGCGCTGCACGCAGAGGCCCAGCGCCACCGGGTATACCGATTGTTGACCAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACg                                                                                               >  2:351631/1‑100 (MQ=255)
           gTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACg                                                                                               <  2:2015026/100‑1 (MQ=255)
                     cGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCgg                                                                                     <  2:2386273/100‑1 (MQ=255)
                     cGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCgg                                                                                     <  2:1965785/100‑1 (MQ=255)
                     cGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCg                                                                                      >  1:2083787/1‑99 (MQ=255)
                      ggtgggCTCTGCACGCAGTGGCACAGCGCGACGGCGTATACAGATTGTTGACAAAGTGCGCTTGGTTTATGCTGGATAAGGCGTTAACGCCCGATCCGGc                                                                                    <  2:1485043/96‑1 (MQ=18)
                      ggATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGc                                                                                    >  1:2031096/1‑100 (MQ=255)
                      ggATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGc                                                                                    <  1:1139263/100‑1 (MQ=255)
                      ggATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGc                                                                                    >  1:533609/1‑100 (MQ=255)
                      ggATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATAAGGCGTTAACGccgcatacggc                                                                                    <  2:868053/100‑10 (MQ=37)
                      ggATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATAAGGCGTTAACGCCGCATCCGGc                                                                                    <  1:2265040/100‑1 (MQ=37)
                       gATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTTTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGcc                                                                                   <  1:1680355/100‑1 (MQ=255)
                       gATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGcc                                                                                   <  1:1026546/100‑1 (MQ=255)
                       gATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGcc                                                                                   <  2:2246673/100‑1 (MQ=255)
                       gATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGcc                                                                                   <  2:257273/100‑1 (MQ=255)
                       gATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATAAGGCGTTAACGCCGCATCCGGca                                                                                   <  1:1321008/100‑2 (MQ=37)
                        aTGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCt                                                                                  <  2:1049687/100‑1 (MQ=255)
                         tGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCGTATCCGGCCTa                                                                                 <  2:583207/100‑1 (MQ=255)
                           gcgcTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑a                                                                              >  2:1289056/1‑100 (MQ=255)
                           gcgcTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑a                                                                              >  1:2233800/1‑100 (MQ=255)
                               tGCACGCAGAGGCACAGCGCCACCGGGTATACAGGTTGTTGACAAAGGGTGCTTTGTTTCTGCTGGATGCGGCGTTAACGCCTTAGCCGCCCTAC‑a                                                                              >  2:1420308/1‑96 (MQ=37)
                                  aCGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGt                                                                       >  1:1906890/1‑100 (MQ=255)
                                    gCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGc                                                                     <  1:1757996/100‑1 (MQ=255)
                                    gCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGc                                                                     <  2:1625146/100‑1 (MQ=255)
                                     cAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCa                                                                    <  2:95124/100‑1 (MQ=255)
                                     cAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCa                                                                    >  2:1598712/1‑100 (MQ=255)
                                      agagGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGc                                                                     <  1:970509/98‑1 (MQ=255)
                                       gagGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCaaa                                                                  >  2:367193/1‑100 (MQ=255)
                                        agGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAAt                                                                 >  2:910870/1‑100 (MQ=255)
                                          gCACAGCGCCACCGGGTATACAGATTGTTGACCAAGTGCGCTGTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTc                                                               <  2:1672339/100‑1 (MQ=255)
                                          gCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTc                                                               <  1:531135/100‑1 (MQ=255)
                                          gCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTc                                                               <  1:649986/100‑1 (MQ=255)
                                           cacaGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCa                                                              <  2:2132931/100‑1 (MQ=255)
                                           cacaGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCa                                                              >  2:1583913/1‑100 (MQ=255)
                                            acaGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCaa                                                             <  1:595832/100‑1 (MQ=255)
                                             caGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAAt                                                            >  1:1205313/1‑100 (MQ=255)
                                               gcgcCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATAc                                                          >  1:791972/1‑100 (MQ=255)
                                                  ccACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACAtt                                                       >  2:2019038/1‑100 (MQ=255)
                                                      cGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGc                                                     <  2:2011285/98‑1 (MQ=255)
                                                      cGGGTATACAGATTGTTCACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCa                                                    <  2:2168621/99‑1 (MQ=255)
                                                        ggTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑Gaa                                                >  2:1997370/1‑100 (MQ=255)
                                                           ataCGGATTGTTGACAAAGTGCGCGTTGTTTAAGCTGGATGCGGCGTTAACGCCTTATCGGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑g                                                  <  2:2214437/95‑1 (MQ=14)
                                                            taCAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GAAt‑ttt                                           >  2:1234753/1‑100 (MQ=255)
                                                             aCAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GAAT‑TTTc                                          >  2:336257/1‑100 (MQ=255)
                                                              cAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GAAT‑TTTC‑‑g                                       <  1:1540601/100‑1 (MQ=255)
                                                                gATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GAAT‑TTTC‑‑GTa                                     <  2:359398/100‑1 (MQ=255)
                                                                 aTTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GAAT‑TTTC‑‑GTAg                                    <  1:913858/100‑1 (MQ=255)
                                                                  gcataaaCAAAGCGCACTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GAAT‑TTTC‑‑GTAgg                                   >  1:1159376/7‑100 (MQ=17)
                                                                  gcataaaCAAAGCGCACTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GAAT‑TTTC‑‑GTAgg                                   >  1:1556835/7‑100 (MQ=18)
                                                                  gcataaaCAAAGCGCACTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GAAT‑TTTC‑‑GTAgg                                   >  1:224687/7‑100 (MQ=18)
                                                                   tgttgACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GAAT‑TTTC‑‑GTAGGc                                  <  2:1541466/100‑1 (MQ=255)
                                                                   tgttgACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GAAT‑TTTC‑‑GTAGGc                                  >  1:1266934/1‑100 (MQ=255)
                                                                     ttgACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GAAT‑TTTC‑‑GTAGGcc                                 <  1:1978294/99‑1 (MQ=255)
                                                                      tgACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GAAT‑TTTC‑‑GTAGGCCTg                               <  1:1234760/100‑1 (MQ=255)
                                                                      tgACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GAAT‑TTTC‑‑GTAGGCCTg                               >  1:389865/1‑100 (MQ=255)
                                                                         cAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GAAT‑TTTC‑‑GTAGGCCTGATa                            <  1:571042/100‑1 (MQ=255)
                                                                          aaatggcgcTTTATATAGGCCGGATGCGGCGTTAACGCCCTATCAGGCCTGC‑AAAAGCGTGCATTTTCACTACATTGCA‑TAAT‑TTTC‑‑GTAGTCCTGATa                            <  2:1548107/94‑1 (MQ=16)
                                                                             gTGCGCTTTGTTTATGCCGGATGCGGCTTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GAAT‑TTTC‑‑GTAGGCCTGATAAGCg                        <  1:992402/100‑1 (MQ=255)
                                                                              tGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTCC‑AAACTCGTGCAAATTCAATACATTGCA‑GAAT‑TTTC‑‑GTAGGCCTGATAAGCgg                       <  2:1544585/100‑1 (MQ=255)
                                                                              tGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GAAT‑TTTC‑‑GTAGGCCTGATAAGCgg                       <  2:1472279/100‑1 (MQ=255)
                                                                               gcgcTTTGTTTATGTCGGATGCGGCGTTAACGCCTTATCCGGGCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GAAT‑TTTC‑‑GTAGGCCTGATAAGCGGa                      <  2:851541/100‑1 (MQ=255)
                                                                                  gattttgtaggCCGGATAAGGCGTTCACGCCGCATCCGGCCTAC‑AAAATCGCTTAAATTCAATATATTGCA‑CCAT‑CCTC‑‑GTAGGCCTGATAAGCGTAgcg                   >  1:1437372/11‑100 (MQ=18)
                                                                                  gaatttgtaggCCGGATAAGGCGTTTACGCCGCATCCGGCCTAC‑AAATTC‑TGCAAATTCAACAAATTGC‑‑GAATCCCTT‑‑GTAGGCCTGATAAGCGTAgcgc                  >  1:1962390/11‑100 (MQ=18)
                                                                                    ttttgtaggCCGGATAAGGCGTTCACGCCGCATCCGGCCTAC‑AAAACTTTGCAAATTCAATATATTGC‑‑‑AAT‑CTCCGTGTAGGCCTGATAAGCGTAGCGCa                 >  1:1368646/9‑100 (MQ=18)
                                                                                    ttGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GAAT‑TTTC‑‑GTAGGCCTGATAAGCGGAGCGCa                 <  1:1472132/100‑1 (MQ=255)
                                                                                      gTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GAAT‑TTTC‑‑GTAGGCCTGATAAGCGGAGCGCATc               >  2:797622/1‑100 (MQ=255)
                                                                                      gTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GAAT‑TTTC‑‑GTAGGCCTGATAAGCGGAGCGCATc               <  2:1065081/100‑1 (MQ=255)
                                                                                       tttATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GAAT‑TTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCa              <  1:1806524/100‑1 (MQ=255)
                                                                                        ttATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GAAT‑TTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAg             >  1:320122/1‑100 (MQ=255)
                                                                                        gtaggCCGGATAAGGCGTTCACGCCGCATCCGGCGTAC‑GAAATCGTGCTAATTCAATATATTGTG‑GGCT‑CCAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAg             >  1:61560/5‑100 (MQ=21)
                                                                                        gtaggCCGGATAAGGCGTTCACGCCGCATCCGGCCTAC‑‑AAATCATGCAAATCCAAAATATTGCA‑GGAG‑TTAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAgg            >  1:1017640/5‑100 (MQ=21)
                                                                                         taggCCGGATAAGGCGTTCACGCCGCATCCGGCCTAC‑AAAAGCATGAAAATTCAATATATTGCA‑GGAG‑CTGC‑‑GTAGGCCTGATAGGCGTAGCGCATCAgg            >  1:2351496/4‑100 (MQ=18)
                                                                                         taggCCGGATAAGGCGTTCACGCCGCATCCGGCCTAC‑‑AAATCATGCAAATCCAAAATATTGCA‑GGAG‑TTAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGc           >  1:2073405/4‑100 (MQ=21)
                                                                                          aggCCGGATAAGGCGTTCACGCCGCATCCGGCCTAC‑AAGTTCGTGCAAATTCAATAAATTGCA‑‑ATA‑TGAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGcg          >  1:1094191/3‑99 (MQ=21)
                                                                                          aggCCGGATAAGGCGTTCACGCCGCATCCGGCCTAC‑AAAAGCATGAAAATTCAATATATTGCA‑GGAG‑CTGC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGc           >  2:1311126/3‑100 (MQ=18)
                                                                                          aggCCGGATAAGGCGTTAACGCCGCATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GAAT‑TTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGc           >  1:2300622/3‑100 (MQ=18)
                                                                                          aTGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GAAT‑TTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGc           >  1:1154672/1‑100 (MQ=255)
                                                                                           ggCCGGATAAGGCGTTTACGCCGCATCCGGCCTAC‑AAAAGCGTGCAAATTCAATACATTGCATGGGC‑CAT‑‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCa          >  1:181213/2‑100 (MQ=17)
                                                                                           ggCCGGATAAGGCGTTCACGCCGCATCCGGCCTAC‑‑AAATCATGCAAATCCAAAATATTGCA‑GGAG‑TTAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAt         >  1:1788110/2‑100 (MQ=21)
                                                                                           ggCCGGATAAGGCGTTAACGCCGCATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GAAT‑TTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCa          >  2:2323033/2‑100 (MQ=18)
                                                                                            gCCGGATGCGGCGTGAACGCCTTATCCGGCCTAT‑AAAGTCGTATAAATTCAATGTATTGCA‑GTTT‑ATTG‑‑GTAGGCCTGATGCGCTGCGCTTATCAGGcct         <  1:464619/100‑3 (MQ=21)
                                                                                            gCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAAATCTTGCCAATTCAATATATTGCA‑GGAC‑CAT‑‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCag         >  1:1884715/1‑99 (MQ=18)
                                                                                            gCCGGATAAGGCGTTCACGCCGCATCCGGCCTAC‑AAAATTGTGTAAATTCAATAAATTGAT‑CGAT‑CCAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCag         >  2:2153720/1‑99 (MQ=18)
                                                                                            gCCGGATAAGGCGTTCACGCCGCATCCGGCCTAC‑AAAAGCATGAAAATTCAATATATTGCA‑GGAG‑CTGC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCag         >  1:1336598/1‑99 (MQ=18)
                                                                                             ccGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GAAT‑TTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCAtt        >  1:150657/1‑100 (MQ=255)
                                                                                             ccGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GAAT‑TTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCAtt        <  1:802835/100‑1 (MQ=255)
                                                                                             ccGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GAAT‑TTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCAt         >  1:2233425/1‑99 (MQ=255)
                                                                                                gATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GAAT‑TTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCAttttt     <  1:1981795/100‑1 (MQ=255)
                                                                                                  tGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GAAT‑TTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgc   >  1:1157620/1‑100 (MQ=255)
                                                                                                  tGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GAAT‑TTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgc   <  1:1555112/100‑1 (MQ=255)
                                                                                                   gcggcgTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GAAT‑TTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgcc  >  1:64634/1‑100 (MQ=255)
                                                                                                   gcggcgTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GAAT‑TTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgcc  >  1:879450/1‑100 (MQ=255)
                                                                                                   |                                                                                                        
CAGGGAATGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GAAT‑TTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCC  >  NC_000913/3705871‑3706069

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A