Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A9 F255 I1 R1	77	120.7	6116409	93.2%	5700493	99.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,304,813	(C)_7→8	coding (1005/1245 nt)	mtr ←	tryptophan transporter of high affinity

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,304,806	1	.	C	93.5%	306.5 / 14.9	93	coding (1012/1245 nt)	mtr	tryptophan transporter of high affinity
Reads supporting (aligned to +/- strand): ref base . (6/0); new base C (47/39); total (53/40)
Fisher's exact test for biased strand distribution p-value = 3.68e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 109 total aligned reads displayed.

GCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAAC‑A‑G‑‑C‑C‑CCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTCA  >  NC_000913/3304707‑3304900

gcgATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAAC‑A‑g                                                                                                    <  2:224752/100‑1 (MQ=255)

gcgATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAAC‑A‑g                                                                                                    <  2:1268332/100‑1 (MQ=255)

 cgATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAAC‑A‑G‑‑c                                                                                                 >  2:2717567/1‑100 (MQ=255)

 cgATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAAC‑A‑G‑‑c                                                                                                 <  1:1081877/100‑1 (MQ=255)

 cgATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAAC‑A‑G‑‑c                                                                                                 >  1:2476364/1‑100 (MQ=255)

   aTGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAAC‑A‑G‑‑c‑c‑c                                                                                             <  1:1348018/100‑1 (MQ=255)

     gCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAAC‑A‑G‑‑c‑c‑ccc                                                                                           <  1:1479541/100‑1 (MQ=255)

      cACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAAC‑A‑G‑‑c‑c‑cccc                                                                                          >  1:2696273/1‑100 (MQ=255)

       aCGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAAC‑A‑G‑‑c‑c‑ccccn                                                                                         <  2:2410755/100‑2 (MQ=255)

       aCGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAAC‑A‑G‑‑c‑c‑ccccc                                                                                         >  2:1326195/1‑100 (MQ=255)

       aCGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAAC‑A‑G‑‑c‑c‑ccccc                                                                                         <  1:467359/100‑1 (MQ=255)

       aCGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAAC‑A‑G‑‑c‑c‑ccccc                                                                                         <  2:2417942/100‑1 (MQ=255)

             tAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAAC‑A‑GC‑C‑C‑CCCCCACAAc                                                                                    <  2:1613457/100‑1 (MQ=255)

             tAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAAC‑A‑GC‑C‑C‑CCCCCACAAc                                                                                    <  2:520351/100‑1 (MQ=255)

              aaCAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAAC‑A‑GC‑C‑C‑CCCCCACAACt                                                                                   <  2:2820676/100‑1 (MQ=255)

                cAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAAC‑A‑GC‑C‑C‑CCCCCACAACTgg                                                                                 >  2:32024/1‑100 (MQ=255)

                cAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAAC‑A‑G‑‑c‑c‑cccccccacaactg                                                                                >  1:2929739/1‑91 (MQ=255)

                cAGCGCCGGAACAATTACCACCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGCTCAGGAACAAC‑ACC‑‑C‑C‑CCCCCACAACTgg                                                                                 >  2:1427259/1‑100 (MQ=255)

                 aGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAAC‑A‑GC‑C‑C‑CCCCCACAACTGGc                                                                                >  2:2399495/1‑100 (MQ=255)

                  gcgcCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAAC‑A‑GC‑C‑C‑CCCCCACAACTGGCg                                                                               <  2:2568415/100‑1 (MQ=255)

                   cgcCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAAC‑A‑GC‑C‑C‑CCCCCACAACTGGCgg                                                                              <  2:337513/100‑1 (MQ=255)

                    gcCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAAC‑A‑GC‑C‑C‑CCCCCACAACTGGCggg                                                                             <  2:350183/100‑1 (MQ=255)

                    gcCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAAC‑A‑GC‑C‑C‑CCCCCACAACTGGCggg                                                                             >  1:245374/1‑100 (MQ=255)

                    gcCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAAC‑A‑GC‑C‑C‑CCCCCACAACTGGCggg                                                                             >  1:131911/1‑100 (MQ=255)

                     ccGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAAC‑A‑GC‑C‑C‑CCCCCACAACTGGCgggg                                                                            >  1:2543917/1‑100 (MQ=255)

                     ccGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAAC‑A‑GC‑C‑C‑CCCCCACAACTGGCgggg                                                                            <  1:1192256/100‑1 (MQ=255)

                     ccGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAAC‑A‑GC‑C‑C‑CCCCCACAACTGGCgggg                                                                            >  2:1546995/1‑100 (MQ=255)

                      cGGAACAATTGCCGGCCAAATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTGGGGAACAAC‑A‑GC‑C‑C‑CCCCCACAGCTGGCGGGGc                                                                           <  2:2134316/100‑1 (MQ=255)

                      cGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAAC‑A‑GC‑C‑C‑CCCCCACAACTGGCGGGGc                                                                           >  1:633401/1‑100 (MQ=255)

                      cGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAAC‑A‑GC‑C‑C‑CCCCCACAACTGGCGGGGc                                                                           >  2:2611126/1‑100 (MQ=255)

                      cGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAAC‑A‑GC‑C‑C‑CCCCCACAACTGGCGGGGc                                                                           >  2:1902455/1‑100 (MQ=255)

                      cGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAAC‑A‑GC‑C‑C‑CCCCCACAACTGGCGGGGc                                                                           >  1:850877/1‑100 (MQ=255)

                      cGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAAC‑A‑G‑‑C‑C‑CCCCCACAACTGGCGGGGCa                                                                          >  1:824319/1‑100 (MQ=255)

                        gAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAAC‑A‑GC‑C‑C‑CCCCCACAACTGGCGGGGCaa                                                                         <  2:2694332/100‑1 (MQ=255)

                        gAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAAC‑A‑GC‑C‑C‑CCCCCACAACTGGCGGGGCaa                                                                         >  1:359531/1‑100 (MQ=255)

                        gAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAAC‑A‑GC‑C‑C‑CCCCCACAACTGGCGGGGCaa                                                                         >  1:2338568/1‑100 (MQ=255)

                         aaCAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAAC‑A‑GC‑C‑C‑CCCCCACAACTGGCGGGGCaaa                                                                        <  1:2522062/100‑1 (MQ=255)

                           cAATTGCCGCCCAGATGGTAGCCGCTAAATCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGATCAAC‑A‑GC‑C‑C‑CCCCCACAACTGGCGGGGCAAAgg                                                                      <  2:1835253/100‑1 (MQ=255)

                           cAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAAC‑A‑G‑‑C‑C‑CCCCCACAACTGGCGGGGCAAAGGt                                                                     >  2:1216826/1‑100 (MQ=255)

                            aaTTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAAC‑A‑GC‑C‑C‑CCCCCACAACTGGCGGGGCAAAGGt                                                                     >  1:886351/1‑100 (MQ=255)

                             aTTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAAC‑A‑GC‑C‑C‑CCCCCACAACTGGCGGGGCAAAGGTc                                                                    >  1:294/1‑100 (MQ=255)

                             aTTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAAC‑A‑GC‑C‑C‑CCCCCACAACTGGCGGGGCAAAGGTc                                                                    >  1:1081364/1‑100 (MQ=255)

                             aTTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAAC‑A‑GC‑C‑C‑CCCCCACAACTGGCGGGGCAAAGGTc                                                                    >  1:1882144/1‑100 (MQ=255)

                               tGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAAC‑A‑GC‑C‑C‑CCCCCACAACTGGCGGGGCAAAGGTCAg                                                                  >  2:2413663/1‑100 (MQ=255)

                                 ccgccCAGATGGTAGCCGCTAAACCAGCATAACCAAGGGCGTACAGGAATCCGTTCGGGAACAAC‑A‑GC‑C‑C‑CCCCCACAACTGGCGGGGCAAAGGTCAGCa                                                                <  1:1160618/100‑1 (MQ=255)

                                  cgccCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAAC‑A‑GC‑C‑C‑CCCCCACAACTGGCGGGGCAAAGGTCAGCaa                                                               >  1:70393/1‑100 (MQ=255)

                                  cgccCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAAC‑A‑GC‑C‑C‑CCCCCACAACTGGCGGGGCAAAGGTCAGCaa                                                               >  1:1226230/1‑100 (MQ=255)

                                    cccAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAAC‑A‑GC‑C‑C‑CCCCCACAACTGGCGGGGCAAAGGTCAGCAATg                                                             >  1:998247/1‑100 (MQ=255)

                                       aGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAA‑C‑‑C‑C‑CCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCg                                                          >  2:2066991/1‑100 (MQ=255)

                                          tGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAAC‑A‑GC‑C‑C‑CCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCg                                                          >  2:1351310/1‑97 (MQ=255)

                                            gTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAAC‑A‑GC‑C‑C‑CCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTc                                                     >  2:342670/1‑100 (MQ=255)

                                             tAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAAC‑A‑GC‑C‑C‑CCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCa                                                    <  2:2056372/100‑1 (MQ=255)

                                             tAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAAC‑A‑GC‑C‑C‑CCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCa                                                    >  2:1846747/1‑100 (MQ=255)

                                             tAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAAC‑A‑GC‑C‑C‑CCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCa                                                    <  2:2476331/100‑1 (MQ=255)

                                              aGCCGCTAAACCGGCATAACCAAGTGCTTACATGAATCCGTTCGGGAACTTC‑A‑GT‑C‑C‑CCCCCACAACTGGCGGGGCAAAGGTCATCAATGCCGttt                                                       <  2:290285/96‑1 (MQ=255)

                                               gCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAAC‑A‑GC‑C‑C‑CCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAg                                                  >  2:794247/1‑100 (MQ=255)

                                                 cGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAAC‑A‑GC‑C‑C‑CCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCg                                                >  2:2823651/1‑100 (MQ=255)

                                                   cTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAAC‑A‑GC‑C‑C‑CCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGc                                              <  2:2925140/100‑1 (MQ=255)

                                                    tAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAAC‑A‑GC‑C‑C‑CCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGcc                                             <  1:1428435/100‑1 (MQ=255)

                                                    tAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAAC‑A‑GC‑C‑C‑CCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGcc                                             <  1:805157/100‑1 (MQ=255)

                                                     aaaCCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAAC‑A‑GC‑C‑C‑CCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGccc                                            >  2:1575931/1‑100 (MQ=255)

                                                       aCCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAAC‑A‑GC‑C‑C‑CCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCcac                                          <  1:2219172/100‑1 (MQ=255)

                                                       aCCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAAC‑A‑GC‑C‑C‑CCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCcac                                          <  1:1925157/100‑1 (MQ=255)

                                                        ccAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAAC‑A‑GC‑C‑C‑CCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCcaca                                         >  2:1489353/1‑100 (MQ=255)

                                                         cAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAAC‑A‑GC‑C‑C‑CCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAg                                        <  1:428457/100‑1 (MQ=255)

                                                          aGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAAC‑A‑GC‑C‑C‑CCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGc                                       <  1:1846769/100‑1 (MQ=255)

                                                            atctaCCAATGGCGTACAGGAATCCGTTCGGGAACAAC‑A‑GC‑C‑C‑CCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCg                                     <  1:2852059/96‑1 (MQ=255)

                                                             aTAACCAATGGCGTACAGGAATCCGTTCGGGAACAAC‑A‑GC‑C‑C‑CCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGa                                    >  2:340237/1‑100 (MQ=255)

                                                             aTAACCAATGGCGTACAGGAATCCGTTCGGGAACAAC‑A‑G‑‑C‑CACCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGa                                    >  2:3041407/1‑100 (MQ=255)

                                                              tAACCAATGGCGTACAGGAATCCGTTCGGGAACAAC‑A‑GCCC‑C‑CCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGa                                    >  2:1774529/1‑100 (MQ=255)

                                                              tAACCAATGGCGTACAGGAATCCGTTCGGGAACAAC‑A‑GC‑C‑C‑CCCCCACAACTTGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAg                                   >  2:798413/1‑100 (MQ=255)

                                                              tAACCAATGGCGTACAGGAATCCGTTCGGGAACAAC‑A‑GC‑C‑C‑CCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAg                                   <  1:2886974/100‑1 (MQ=255)

                                                                aCCAATGGCGTACAGGAATCCGTTCGGGAACAAC‑A‑GC‑C‑C‑CCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAgat                                 >  2:2852020/1‑98 (MQ=255)

                                                                 ccAATGGCGTACAGGAATCCGTTCGGGAACAAC‑A‑GC‑C‑C‑CCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAgtcg                                <  1:32026/100‑1 (MQ=255)

                                                                  cAATGGCGTACAGGAATCCGTTCGGGAACAAC‑A‑GC‑C‑C‑CCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAgtcgt                               >  2:2307910/1‑100 (MQ=255)

                                                                    aTGGCGTACAGGAATCCGTTCGGGAACAAC‑A‑GC‑C‑C‑CCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGtcgtcg                             >  2:2164677/1‑100 (MQ=255)

                                                                    aTGGCGTACAGGAATCCGTTCGGGAACAAC‑A‑GC‑C‑C‑CCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGtcgtcg                             <  2:756316/100‑1 (MQ=255)

                                                                       gCGTACAGGAATCCGTTCGGGAACAAC‑A‑GC‑C‑C‑CCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGaaa                          <  1:1935453/100‑1 (MQ=255)

                                                                          tACAGGAATCCGTTCGGGAACAAC‑A‑GC‑C‑C‑CCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCa                       <  2:225404/100‑1 (MQ=255)

                                                                             aGGAATCCGTTCGGGAACAAC‑A‑GC‑C‑C‑CCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAAc                    <  1:299670/100‑1 (MQ=255)

                                                                             aGGAATCCGTTCGGGAACAAC‑A‑GC‑C‑C‑CCCCCACAACTGGCGGCGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAAc                    <  2:850867/100‑1 (MQ=255)

                                                                              ggAATCCGTTCGGGAACAAC‑A‑GC‑C‑C‑CCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACa                   >  2:927568/1‑100 (MQ=255)

                                                                              ggAATCCGTTCGGGAACAAC‑A‑GC‑C‑C‑CCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACa                   >  1:818954/1‑100 (MQ=255)

                                                                                aaTCCGTTCGGGAACAAC‑A‑GC‑C‑C‑CCCCCACAACTGGCGGGGCCAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGa                 <  2:1206862/100‑1 (MQ=255)

                                                                                 aTCCGTTCGGGAACAAC‑A‑GC‑C‑C‑CCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGAt                <  2:633393/100‑1 (MQ=255)

                                                                                   ccGTTCGGGAACAAC‑A‑GC‑C‑C‑CCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCt              >  1:1024200/1‑100 (MQ=255)

                                                                                   ccGTTCGGGAACAAC‑A‑GC‑C‑C‑CCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCt              >  2:2830938/1‑100 (MQ=255)

                                                                                   ccGTTCGGGAACAAC‑A‑GC‑C‑C‑CCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCt              >  1:2013619/1‑100 (MQ=255)

                                                                                   ccGTTCGGGAACAAC‑A‑GC‑C‑C‑CCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCt              <  1:1575949/100‑1 (MQ=255)

                                                                                    cGTTCGGGAACAAC‑A‑G‑‑CGT‑CCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGCCGTCAAAACCAAACaca                 >  2:2532950/1‑94 (MQ=255)

                                                                                     gTTCGGGAACAAC‑A‑GC‑C‑C‑CCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGc            >  1:1028527/1‑100 (MQ=255)

                                                                                      ttCGGGAACAAC‑A‑GC‑C‑C‑CCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGcc           >  1:507335/1‑100 (MQ=255)

                                                                                      ttCGGGAACAAC‑A‑GC‑C‑C‑CCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGcc           <  1:1902476/100‑1 (MQ=255)

                                                                                        cGGGAACAAC‑A‑GC‑C‑C‑CCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCa          >  1:2695645/1‑99 (MQ=255)

                                                                                        cGGGAACAAC‑A‑GC‑C‑C‑CCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCAGAGTCGTCGAAACCAAACAGATCTGCCAg         >  2:1917797/1‑100 (MQ=255)

                                                                                         gggAACAAC‑A‑GC‑C‑C‑CCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGa        >  1:583131/1‑100 (MQ=255)

                                                                                          ggAACAAC‑A‑GC‑C‑C‑CCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGAt       <  2:807956/100‑1 (MQ=255)

                                                                                             acaac‑a‑GC‑C‑C‑CCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGt    >  2:451472/1‑100 (MQ=255)

                                                                                             acaac‑a‑GC‑C‑C‑CCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGt    <  2:2696237/100‑1 (MQ=255)

                                                                                               aac‑a‑GC‑C‑C‑CCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTca  <  2:359524/100‑1 (MQ=255)

GCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAAC‑A‑G‑‑C‑C‑CCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTCA  >  NC_000913/3304707‑3304900

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A