Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F228 I0 R1 182 117.2 5818924 93.6% 5446512 99.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,215,178 Δ1 bp 100% coding (899/930 nt) metA → homoserine O‑transsuccinylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,215,1780A.94.2% 556.4 / 25.4 137coding (899/930 nt)metAhomoserine O‑transsuccinylase
Reads supporting (aligned to +/- strand):  ref base A (4/4);  new base . (75/54);  total (79/58)
Fisher's exact test for biased strand distribution p-value = 7.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 137 total aligned reads displayed.

TCCGCAAAATACACCGCGAGCGAGCTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACGATCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTT  >  NC_000913/4215083‑4215272
                                                                                               |                                                                                              
tCCGCAAAATACACCGCGAGCGAGCTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACGATc                                                                                              <  1:792218/98‑1 (MQ=255)
tCCGCAAAATACACCGCGAGCGAGCTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACG‑TCTAc                                                                                           >  1:1302415/1‑100 (MQ=255)
tCCGCAAAATACACCGCGAGCGAGCTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACG‑TCTAc                                                                                           <  1:739318/100‑1 (MQ=255)
  cGCAAAATACACCGCGAGCGAGCTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACG‑TCTACgg                                                                                         >  1:1039155/1‑100 (MQ=255)
  cGCAAAATACACCGCGAGCGAGCTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACG‑TCTACgg                                                                                         >  1:2351817/1‑100 (MQ=255)
   gCAAAATACACCGCGAGCGAGCTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACG‑TCTACGGc                                                                                        <  1:399891/100‑1 (MQ=255)
    cAAAATACACCGCGAGCGAGCTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACG‑TCTACGGca                                                                                       >  1:2680775/1‑100 (MQ=255)
    cAAAATACACCGCGAGCGAGCTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACG‑TCTACGGca                                                                                       >  1:1907516/1‑100 (MQ=255)
      aaaTACACCGCGAGCGAGCTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACG‑TCTACGGcaca                                                                                     >  2:1911039/1‑100 (MQ=255)
       aaTACACCGCGAGCGAGCTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACG‑TCTACGGCACAt                                                                                    <  1:2380853/100‑1 (MQ=255)
        aTACCCCGCGGGCGAGCTGGCGTAGTCACGGTAATTTACCGTTTACCGACTGGCTCAACTATTACGTCTACCAGATCACGCCATACG‑TCTACGGCACATg                                                                                   <  1:1104558/100‑1 (MQ=255)
            acCGCGAGCGAGCTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACG‑TCTACGGCACATGAATc                                                                               >  2:1317705/1‑100 (MQ=255)
            acCGCGAGCGAGCTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACG‑TCTACGGCACATGAATc                                                                               <  1:2881635/100‑1 (MQ=255)
             ccGCGAGCGAGCTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACG‑TCTACGGCACATGAATcc                                                                              >  2:1897702/1‑100 (MQ=255)
             ccGCGAGCGAGCTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACG‑TCTACGGCACATGAATcc                                                                              <  1:512068/100‑1 (MQ=255)
             ccGCGAGCGAGCTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACG‑TCTACGGCACATGAATcc                                                                              >  2:2813992/1‑100 (MQ=255)
                cgagcgagCTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACG‑TCTACGGCACATGAATCCAAc                                                                           >  2:1774320/1‑100 (MQ=255)
                  agcgagcTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACG‑TCTACGGCACATGAATCCAACGc                                                                         >  2:1457592/1‑100 (MQ=255)
                    cgagcTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACG‑TCTACGGCACATGAATCCAACGCTg                                                                       >  1:1411381/1‑100 (MQ=255)
                     gagcTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTAGCAGATCACGCCATACG‑TCTACGGCACATGAATCCAACGCTg                                                                       <  1:1581767/99‑1 (MQ=255)
                      agcTGGCGTAGTCACGGTCATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACG‑TCTACGGCACATGAATCCAACGCTgca                                                                     >  1:2497418/1‑98 (MQ=255)
                       gcTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACG‑TCTACGGCACATGAATCCAACGCTGGAt                                                                    <  2:742163/100‑1 (MQ=255)
                        cTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACG‑TCTACGGCACATGAATCCAACGCTGGAtt                                                                   <  1:197299/100‑1 (MQ=255)
                         tGGCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACG‑TCTACGGCACATGAATCCAACGCTGGATTa                                                                  >  2:1306151/1‑100 (MQ=255)
                          ggCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACG‑TCTACGGCACATGAATCCAACGCTGGATTaa                                                                 >  1:194333/1‑100 (MQ=255)
                          ggCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACG‑TCTACGGCACATGAATCCAACGCTGGATTaa                                                                 >  2:2781831/1‑100 (MQ=255)
                            cggaGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACG‑TCTACGGCACATGAATCCAACGCTGGATTAAtc                                                               <  1:295174/97‑1 (MQ=255)
                            cGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACG‑TCTACGGCACATGAATCCAACGCTGGATTAAtc                                                               >  2:534407/1‑100 (MQ=255)
                            cGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACG‑TCTACGGCACATGAATCCAACGCTGGATTAAtc                                                               >  2:759569/1‑100 (MQ=255)
                            cGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACG‑TCTACGGCACATGAATCCAACGCTGGATTAAtc                                                               >  2:708263/1‑100 (MQ=255)
                                 tCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACG‑TCTACGGCACATGAATCCAACGCTGGATTAATCTTCtg                                                          >  1:1102062/1‑100 (MQ=255)
                                 tCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACG‑TCTACGGCACATGAATCCAACGCTGGATTAATCTTCtg                                                          <  2:1204377/100‑1 (MQ=255)
                                  cACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACG‑TCTACGGCACATGAATCCAACGCTGGATTAATCTTCtgt                                                         <  1:2376548/100‑1 (MQ=255)
                                  cACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACG‑TCTACGGCACATGAATCCAACGCTGGATTAATCTTCtgt                                                         <  1:1405495/100‑1 (MQ=255)
                                   aCGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACG‑TCTACGGCACATGAATCCAACGCTGGATTAATCTTCtgtg                                                        <  2:1022962/100‑1 (MQ=255)
                                     tcttccgatcTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACG‑TCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGAt                                                      <  1:1121347/91‑1 (MQ=255)
                                       tAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACG‑TCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAg                                                    <  2:2785614/100‑1 (MQ=255)
                                         aTTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACG‑TCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTc                                                  <  2:194335/100‑1 (MQ=255)
                                          tttACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACG‑TCTACGGCACATGAATCCAACGCTGGATTAATCTTCtgtagatcgga                                                 >  2:512379/1‑92 (MQ=255)
                                          tttACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACG‑TCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTc                                                  <  2:1654692/99‑1 (MQ=255)
                                          tttACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACG‑TCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCg                                                 >  2:1588170/1‑100 (MQ=255)
                                             aCTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACG‑TCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATc                                              <  2:1356501/100‑1 (MQ=255)
                                              cTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACG‑TCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCg                                             >  2:2584147/1‑100 (MQ=255)
                                              cTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACG‑TCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCg                                             <  1:958837/100‑1 (MQ=255)
                                               tGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACG‑TCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGt                                            <  2:2147532/100‑1 (MQ=255)
                                                gTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACG‑TCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAgatcggaaga                                           >  2:1121349/1‑91 (MQ=255)
                                                gTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACG‑TCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGtt                                           >  1:2307450/1‑100 (MQ=255)
                                                gTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACG‑TCTACCGCACATGAATCCAACGCCGCATTAATCTTCCGTGATAGTCGacc                                              >  1:2589185/1‑95 (MQ=255)
                                                 tttACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACG‑TCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTa                                          <  2:2181865/100‑1 (MQ=255)
                                                  ttACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACG‑TCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTaa                                         >  2:1443314/1‑100 (MQ=255)
                                                   tACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACG‑TCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAg                                        <  2:82453/100‑1 (MQ=255)
                                                    aCCAACTGGCTCAACTATTACGTCTACCAGGTCACGCCATACG‑TCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGGGATAGTCGATCGTTAAGc                                       >  2:2613212/1‑100 (MQ=255)
                                                     ccAACTGGCTCACCTACTACGTCTACCAGATCACTCCAT‑CCATCTGCGGCACATCAATCCAATGCTGCAGTAATCGTCTGCGATAAACGAGCGCTAAGc                                       >  1:1904016/1‑99 (MQ=255)
                                                     ccAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACGATCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGc                                       >  1:460046/1‑100 (MQ=255)
                                                     ccAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACG‑TCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCg                                      >  2:2127852/1‑100 (MQ=255)
                                                      cAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACG‑TCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGa                                     >  1:706397/1‑100 (MQ=255)
                                                       aaCTGGCTCAACTATTACGTCTACCAGATCACGCCATACG‑TCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGAt                                    >  1:427921/1‑100 (MQ=255)
                                                        aCTGGCTCAACTATTACGTCTACCAGATCACGCCATACG‑TCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGAtt                                   >  1:1576478/1‑100 (MQ=255)
                                                        aCTGGCTCAACTATTACGTCTACCAGATCACGCCATACG‑‑‑‑ACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAg                                <  2:2320333/100‑1 (MQ=255)
                                                         cTGGCTCAACTATTACGTCTACCAGATCACGCCATACG‑TCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTc                                  >  2:1174215/1‑100 (MQ=255)
                                                         cTGGCTCAACTATTACGTCTACCAGATCACGCCATACG‑TCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTc                                  <  2:685374/100‑1 (MQ=255)
                                                           ggCTCAACTATTACGTCTACCAGATCACGCCATACG‑TCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAg                                <  2:1481076/100‑1 (MQ=255)
                                                            gCTCAACTATTACGTCTACCAGATCACGCCATACG‑TCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTAGATCGTTAAGAGATTCAGc                               >  2:1694788/1‑100 (MQ=255)
                                                             ntCAACTATTACGTCTACCAGATCACGCCATACG‑TCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGACCGTTAAGCGATTCAGCa                              >  1:808199/2‑100 (MQ=255)
                                                             cTCAACTATTACGTCTACCAGATCACGCCATACG‑TCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCa                              >  1:1711622/1‑100 (MQ=255)
                                                              tCAATTATTACGTCAAACAGCTCCAGCAATAC‑ATCTAGGGCCTATGAAACCACCGCTGCATTAACATCATGTGATAGTAGATCCTTAAGCGATTCAGCa                              <  1:1694783/99‑1 (MQ=255)
                                                              tCAACTATTACGTCTACCATATCACGCCACACG‑TCTACGTCACATTAATCCAACGCTCCATTACTCTTCTGTGATAGTCGCTCGTTACGCGATTCAGCAc                             <  1:1008112/100‑1 (MQ=255)
                                                              tCAACTATTACGTCTACCAGATCACGCCATACG‑TCTACGGCACATGAATGCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCAc                             <  2:2497429/100‑1 (MQ=255)
                                                              tCAACTATTACGTCTACCAGATCACGCCATACG‑TCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCAc                             <  2:380300/100‑1 (MQ=255)
                                                              tCAACTATTACGTCTACCAGATCACGCCATACG‑TCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCAc                             <  1:96716/100‑1 (MQ=255)
                                                               cAACTATTACGTCTACCAGATCACGCCATACG‑TCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCAcc                            >  2:335282/1‑100 (MQ=255)
                                                                  cTATTACGTCTACCAGATCACGCCATACG‑TCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTa                         <  2:1802793/100‑1 (MQ=255)
                                                                   tATTACGTCTACCAGATCACGCCATACG‑TCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTAc                        >  1:2222230/1‑100 (MQ=255)
                                                                   tATTACGTCTACCAGATCACGCCATACG‑TCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTAc                        >  1:2298309/1‑100 (MQ=255)
                                                                    aTTACGTCTACCAGATCACGCCATACGATCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTAc                        >  1:1490791/1‑100 (MQ=255)
                                                                    aTTACGTCTACCAGATCACGCCATACG‑TCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTAcc                       >  1:321813/1‑100 (MQ=255)
                                                                    aTTACGTCTACCAGATCACGCCATACG‑TCTACGGCACATGAATCCAACGCTGGAATAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTAc                        >  1:248149/1‑99 (MQ=255)
                                                                     ttACGTCTACCAGATCACGCCATACG‑TCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCt                      <  2:662224/100‑1 (MQ=255)
                                                                     ttACGTCTACCAGATCACGCCATACG‑TCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCt                      <  2:657048/100‑1 (MQ=255)
                                                                      tACGTCTACCAGATCACGCCATACG‑TCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTc                     >  2:1707147/1‑100 (MQ=255)
                                                                      tACGTCTACCAGATCACGCCATACG‑TCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTc                     >  1:149232/1‑100 (MQ=255)
                                                                      tACGTCTACCAGATCACGCCATACG‑TCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTc                     >  1:525032/1‑100 (MQ=255)
                                                                       aCGTCTACCAGATCACGCCATACG‑TCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCa                    <  2:337071/100‑1 (MQ=255)
                                                                        cGTCTACCAGATCACGCCATACG‑TCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAg                   >  2:1559152/1‑100 (MQ=255)
                                                                        cGTCTACCAGATCACGCCATACG‑TCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAg                   <  1:1739252/100‑1 (MQ=255)
                                                                        cGTCTACCAGATCACGCCATACG‑TCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAg                   <  1:538577/100‑1 (MQ=255)
                                                                        cGTCTACCAGATCACGCCATACG‑TCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAg                   <  1:538484/100‑1 (MQ=255)
                                                                         gTCTACCAGATCACGCCATACG‑TCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAgg                  >  2:866724/1‑100 (MQ=255)
                                                                         gTCTACCAGATCACGCCATACG‑TCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAgg                  >  2:2876899/1‑100 (MQ=255)
                                                                          tCTACCAGATCACGCCATACG‑TCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGGTCGTTAAGCGCTTCAGCACCTTACCTCa                    >  1:895718/1‑97 (MQ=255)
                                                                           cTACCAGATCACGCCATACG‑TCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCa                <  2:1411383/100‑1 (MQ=255)
                                                                             aCCAGATCACGCCATACG‑TCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCAcc              >  2:205182/1‑100 (MQ=255)
                                                                              ccAGATCACGCCATACG‑TCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCt             >  1:1983172/1‑100 (MQ=255)
                                                                                aGATCACGCCATACG‑TCTACGGCACATGAATCCAACGCTGGATTAATCCTCTGTGATAGTCGATCGTTAAGCGCTTCAGCACCTTACATCAGGCACCTTc           >  1:107479/1‑100 (MQ=255)
                                                                                   tCACGCCATACG‑TCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCggg        <  2:2843148/100‑1 (MQ=255)
                                                                                   tCACGCCATACG‑TCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCggg        <  1:2881839/100‑1 (MQ=255)
                                                                                    cACGCCATACG‑TCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGt       >  2:1268948/1‑100 (MQ=255)
                                                                                     aCGCCATACG‑TCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTg      <  1:2540145/100‑1 (MQ=255)
                                                                                     aCGCCATACG‑TCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTg      <  2:1821860/100‑1 (MQ=255)
                                                                                         cATACG‑TCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCtt  >  1:1596612/1‑100 (MQ=255)
                                                                                               |                                                                                              
TCCGCAAAATACACCGCGAGCGAGCTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACGATCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTT  >  NC_000913/4215083‑4215272

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A