Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A10 F169 I1 R1 59 169.8 8365175 96.8% 8097489 99.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,215,178 Δ1 bp coding (899/930 nt) metA → homoserine O‑transsuccinylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,215,1780A.97.5% 706.8 / 9.8 161coding (899/930 nt)metAhomoserine O‑transsuccinylase
Reads supporting (aligned to +/- strand):  ref base A (1/3);  new base . (80/77);  total (81/80)
Fisher's exact test for biased strand distribution p-value = 3.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01

Only 100 of 161 total aligned reads displayed.

TGATCCGCAAAATACACCGCGAGCGAGCTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACGATCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTT  >  NC_000913/4215080‑4215272
                                                                                                  |                                                                                              
tGATCCGCAAAATACACCGCGAGCGAGCTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACGAt                                                                                               >  2:120999/1‑100 (MQ=255)
   tCCGCAAAATACACCGCGAGCGAGCTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACG‑TCTAc                                                                                           >  1:2754291/1‑100 (MQ=255)
    ccGCAAAATACACCGCGAGCGAGCTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACG‑TCTACg                                                                                          >  1:4015392/1‑100 (MQ=255)
     cGCAAAATACACCGCGAGCGAGCTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACG‑TCTACgg                                                                                         >  2:289305/1‑100 (MQ=255)
     cGCAAAATACACCGCGAGCGAGCTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACG‑TCTACgg                                                                                         >  1:3110712/1‑100 (MQ=255)
       cAAAATACACCGCGAGCGAGCTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACG‑TCTACGGca                                                                                       <  1:3930744/100‑1 (MQ=255)
        aaaaTACACCGCGAGCGAGCTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACG‑TCTACGGcac                                                                                      <  2:1892943/100‑1 (MQ=255)
        aaaaTACACCGCGAGCGAGCTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACG‑TCTACGGcac                                                                                      <  2:350591/100‑1 (MQ=255)
           aTACACCGCGAGCGAGCTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACG‑TCTACGGCACATg                                                                                   <  2:665842/100‑1 (MQ=255)
            tACACCGCGAGCGAGCTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACG‑TCTACGGCACACGa                                                                                  <  2:2145523/100‑1 (MQ=255)
             acacCGCGAGCGAGCTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACG‑TCTACGGCACATGaa                                                                                 >  1:1693356/1‑100 (MQ=255)
              cacCGCGAGCGAGCTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACG‑TCTACGGCACATGAAt                                                                                <  1:561013/100‑1 (MQ=255)
               acCGCGAGCGAGCTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACGATCTACGGCACATGAAt                                                                                <  1:2374619/100‑1 (MQ=255)
               acCGCGAGCGAGCTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACG‑TCTACGGCACATGAATc                                                                               <  1:2611656/100‑1 (MQ=255)
               acCGCGAGCGAGCTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACG‑TCTACGGCACATGAATc                                                                               <  2:2255970/100‑1 (MQ=255)
                ccGCGAGCGAGCTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACG‑TCTACGGCACATGAATcc                                                                              <  2:1930922/100‑1 (MQ=255)
                  gcgagcgaGCTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACG‑TCTACGGCACATGAATCCaa                                                                            <  2:614496/100‑1 (MQ=255)
                   cgagcgagCTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACG‑TCTACGGCACATGAATCCAAc                                                                           <  1:2847519/100‑1 (MQ=255)
                     agcgagcTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACG‑TCTACGGCACATGAATCCAACGc                                                                         <  2:1633164/100‑1 (MQ=255)
                     agcgagcTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACG‑TCTACGGCACATGAATCCAACGc                                                                         <  1:2703009/100‑1 (MQ=255)
                       cgagcTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACG‑TCTACGGCACATGAATCCAACGCTg                                                                       >  2:3475422/1‑100 (MQ=255)
                       cgagcTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACG‑TCTACGGCACATGAATCCAACGCTg                                                                       >  2:932892/1‑100 (MQ=255)
                        gagcTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACG‑TCTACGGCACATGAATCCAACGCTgg                                                                      >  1:928761/1‑100 (MQ=255)
                        gagcTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACG‑TCTACGGCACATGAATCCAACGCTgg                                                                      >  1:2348573/1‑100 (MQ=255)
                          gcTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACG‑TCTACGGCACATGAATCCAACGCTGGAt                                                                    <  2:727334/100‑1 (MQ=255)
                           cTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACG‑TCTACGGCACATGAATCCAACGCTGGAtt                                                                   <  2:1324321/100‑1 (MQ=255)
                           cTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACG‑TCTACGGCACATGAATCCAACGCTGGAtt                                                                   <  2:3721609/100‑1 (MQ=255)
                             ggCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACG‑TCTACGGCACATGAATCCAACGCTGGATTaa                                                                 >  1:1765967/1‑100 (MQ=255)
                              gCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACG‑TCTACGGCACATGAATCCAACGCTGGATTAAt                                                                <  2:214240/100‑1 (MQ=255)
                               cGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACG‑TCTACGGCACATGAATCCAACGCTGGATTAAtc                                                               <  1:3466135/100‑1 (MQ=255)
                               cGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACG‑TCTACGGCACATGAATCCAACGCTGGATTAAtc                                                               >  2:360815/1‑100 (MQ=255)
                                  aGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACG‑TCTACGGCACATGAATCCAACGCTGGATTAAtcttc                                                            >  2:73327/1‑100 (MQ=255)
                                   gTCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACG‑TCTACGGCACATGAATCCAACGCTGGATTAAtcttct                                                           >  1:2139728/1‑100 (MQ=255)
                                   gTCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACG‑TCTACGGCACATGAATCCAACGCTGGATTAAtcttct                                                           <  1:4052538/100‑1 (MQ=255)
                                   gTCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACG‑TCTACGGCACATGAATCCAACGCTGGATTAAtcttct                                                           <  2:2359922/100‑1 (MQ=255)
                                     cACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACG‑TCTACGGCACATGAATCCAACGCTGGATTAATCTTCtgt                                                         <  2:3070307/100‑1 (MQ=255)
                                     cACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACG‑TCTACGGCACATGAATCCAACGCTGGATTAATCTTCtgt                                                         >  2:3378365/1‑100 (MQ=255)
                                     cACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACG‑TCTACGGCACATGAATCCAACGCTGGATTAATCTTCtgt                                                         <  1:2424628/100‑1 (MQ=255)
                                     cACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACG‑TCTACGGCACATGAATCCAACGCTGGATTAATCTTCtgt                                                         <  2:1538184/100‑1 (MQ=255)
                                     cACGGTAATTCACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACG‑TCTACGGCCCATGAATCCAACGCTGGATTAATCTTCtgt                                                         <  2:2753065/100‑1 (MQ=255)
                                      aCGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACG‑TCTACGGCACATGAATCCAACGCTGGATTAATCTTCtgtg                                                        <  2:622103/100‑1 (MQ=255)
                                       cGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACG‑TCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGa                                                       >  1:2422525/1‑100 (MQ=255)
                                            aTTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACG‑TCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTc                                                  >  1:2623880/1‑100 (MQ=255)
                                            aTTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACG‑TCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTc                                                  <  2:1302232/100‑1 (MQ=255)
                                            aTTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACG‑TCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTc                                                  >  2:2234982/1‑100 (MQ=255)
                                              ttACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACG‑TCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCg                                                 <  2:3926963/99‑1 (MQ=255)
                                                 cTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACG‑TCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCg                                             <  1:541429/100‑1 (MQ=255)
                                                    tttACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACG‑TCTACGGCACATGAATCCAACGCTGGTTTAATCTTCTGTGATAGTCGATCGtt                                           >  1:1101834/1‑99 (MQ=255)
                                                    tttACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACG‑TCTACGGCACATGAATCCAACGCTGGATTCATCTTCTGTGATAGTCGATCGTTa                                          >  1:1548629/1‑100 (MQ=255)
                                                    tttACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACG‑TCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTa                                          <  2:1101827/100‑1 (MQ=255)
                                                    tttACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACG‑TCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTa                                          <  2:1794693/100‑1 (MQ=255)
                                                      tACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACG‑TCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAg                                        >  2:3723096/1‑100 (MQ=255)
                                                       aCCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACG‑TCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGc                                       >  2:1038749/1‑100 (MQ=255)
                                                        ccAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACG‑TCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCg                                      >  1:1642928/1‑100 (MQ=255)
                                                        ccAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACG‑TCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCg                                      <  1:2233933/100‑1 (MQ=255)
                                                        ccAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACG‑TCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCg                                      >  2:3002282/1‑100 (MQ=255)
                                                        ccAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACG‑TCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCg                                      <  1:2922673/100‑1 (MQ=255)
                                                         cAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACG‑TCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGa                                     >  1:1510735/1‑100 (MQ=255)
                                                         cAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACG‑TCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGa                                     >  2:2445954/1‑100 (MQ=255)
                                                          aaCTGGCTCAACTATTACGTCTACCAGATCACGCCATACG‑TCTACGGCACCTGAATCAAACGCTGGATTAATCTTCTGTGCTAGTCGATCGTTACGCGAt                                    >  1:1024251/1‑100 (MQ=255)
                                                            atctcTCAACTATTACGTCTACCAGATCACGCCATACG‑TCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTc                                  <  1:80692/96‑1 (MQ=255)
                                                             tGGCTCAACTATTACGTCTACCAGATCACGCCATACG‑TCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCa                                 <  1:3077068/100‑1 (MQ=255)
                                                              ggCTCAACTATTACGTCTACCAGATCACGCCATACG‑TCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAg                                >  1:3512563/1‑100 (MQ=255)
                                                               gCTCAACTATTACGTCTACCAGATCACGCCATACG‑TCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGc                               <  2:3477721/100‑1 (MQ=255)
                                                                cTCAACTATTACGTCTACCAGATCACGCCATACG‑TCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAgat                              >  2:80692/1‑98 (MQ=255)
                                                                cTCAACTATTACGTCTACCAGATCACGCCATACG‑TCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCa                              >  1:89106/1‑100 (MQ=255)
                                                                  cAACTATTACGTCTACCAGATCACGCCATACG‑TCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCAcc                            >  1:3313632/1‑100 (MQ=255)
                                                                  cAACTATTACGTCTACCAGATCACGCCATACG‑TCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCAcc                            >  1:2341636/1‑100 (MQ=255)
                                                                  cAACTATTACGTCTACCAGATCACGCCATACG‑TCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGAAAGTCGATCGTTAAGCGATTCAGCAcc                            >  2:1996550/1‑100 (MQ=255)
                                                                   aaCTATTACGTCTACCAGATCACGCCATACG‑TCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCt                           >  1:1676801/1‑100 (MQ=255)
                                                                   aaCTATTACGTCTACCAGATCACGCCATACG‑TCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCt                           <  1:1851886/100‑1 (MQ=255)
                                                                     cTATTACGTCTACCAGATCACGCCATACG‑TCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTa                         <  1:998959/100‑1 (MQ=255)
                                                                      tATTACGTCTACCAGATCACGCCATACG‑TCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTAc                        >  1:1354403/1‑100 (MQ=255)
                                                                       aTTACGTCTATCAGATCACGCCATACG‑TCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTTATTGTCGATCGTTAAGCGATACAGCACCTTAcc                       <  2:2742754/100‑1 (MQ=255)
                                                                         tACGTCTACCAGATCACGCCATACG‑TCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTc                     >  1:2095377/1‑100 (MQ=255)
                                                                         tACGTCTACCAGATCACGCCATACG‑TCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTc                     >  2:2641537/1‑100 (MQ=255)
                                                                          aCGTCTACCAGATCACGCCATACG‑TCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCa                    <  2:2870621/100‑1 (MQ=255)
                                                                           cGTCTACCAGATCACGCCATACGATCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCa                    <  1:3760993/100‑1 (MQ=255)
                                                                           cGTCTACCAGATCACGCCATACG‑TCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCa                    >  2:117587/1‑99 (MQ=255)
                                                                             tCTACCAGATCACGCCATACG‑TCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGc                 <  2:294646/100‑1 (MQ=255)
                                                                             tCTACCAGATCACGCCATACG‑TCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGc                 >  1:70244/1‑100 (MQ=255)
                                                                              cTACCAGATCACGCCATACG‑TCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCa                >  1:507524/1‑100 (MQ=255)
                                                                               tACCAGATCACGCCATACG‑TCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCAc               >  2:3849295/1‑100 (MQ=255)
                                                                                aCCAGATCACGCCATACG‑TCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCAcc              >  1:65946/1‑100 (MQ=255)
                                                                                aCCAGATCACGCCATACG‑TCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCAcc              >  2:3590428/1‑100 (MQ=255)
                                                                                aCCAGATCACGCCATACG‑TCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCAcc              >  2:1187600/1‑100 (MQ=255)
                                                                                 ccAGATCACGCCATACG‑TCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCt             >  2:3196086/1‑100 (MQ=255)
                                                                                   aGATCACGCCATACG‑TCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTc           >  1:2689049/1‑100 (MQ=255)
                                                                                    gATCACGCCATACG‑TCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCg          >  1:499819/1‑100 (MQ=255)
                                                                                    gATCACGCCATACG‑TCTACGGCACATGAATCCAACGCTGGAATACACTTCTGTGATAGTCGATCGATAAGAGATTCAGCACCTTACCTCAGGCACCTTCg          >  2:3354518/1‑100 (MQ=255)
                                                                                     aTCACGCCATACG‑TCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCgg         >  2:2447906/1‑100 (MQ=255)
                                                                                     aTCACGCCATACG‑TCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCgg         >  2:2379614/1‑100 (MQ=255)
                                                                                      tCACGCCATACG‑TCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCggg        <  1:3795564/100‑1 (MQ=255)
                                                                                      tCACGCCATACG‑TCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCggg        >  1:3117647/1‑100 (MQ=255)
                                                                                       cACGCCATACG‑TCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGt       <  2:2337799/100‑1 (MQ=255)
                                                                                       cACGCCATACG‑TCTACGGCACATGAATCCAACGCTGGATTAATCATCTGTGATCGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCggg        >  1:2170357/1‑99 (MQ=255)
                                                                                         cGCCATACG‑TCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGc     >  1:171115/1‑100 (MQ=255)
                                                                                         cGCCATACG‑TCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGc     <  1:2172622/100‑1 (MQ=255)
                                                                                         cGCCATACG‑TCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGc     >  1:2446764/1‑100 (MQ=255)
                                                                                             aTACGATCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCt   <  1:120999/99‑1 (MQ=255)
                                                                                                  |                                                                                              
TGATCCGCAAAATACACCGCGAGCGAGCTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACGATCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTT  >  NC_000913/4215080‑4215272

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A