Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A8 F232 I1 R1 72 114.8 5816246 94.5% 5496352 99.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,121,597 (T)5→4 coding (151/1332 nt) hslU ← molecular chaperone and ATPase component of HslUV protease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,121,5930T.97.1% 445.6 / 7.5 105coding (155/1332 nt)hslUmolecular chaperone and ATPase component of HslUV protease
Reads supporting (aligned to +/- strand):  ref base T (2/1);  new base . (49/52);  total (52/53)
Fisher's exact test for biased strand distribution p-value = 6.14e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.31e-01

Only 100 of 118 total aligned reads displayed.

TGGTCGCTTCAACTTTGATGAACGGCGCATTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATATTTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTCC  >  NC_000913/4121495‑4121688
                                                                                                  |                                                                                               
tGGTCGCTTCAACTTTGATGAACGGCGCATTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATAtt                                                                                                >  2:1320697/1‑100 (MQ=255)
 ggTCGCTTCAACTTTGATGAACGGCGCATTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATAttt                                                                                               >  2:1353963/1‑100 (MQ=255)
 ggTCGCTTCAACTTTGATGAACGGCGCATTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATAttt                                                                                               >  2:1712481/1‑100 (MQ=255)
   tCGCTTCAACTTTGATGAACGGCGCATTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATAttttc                                                                                             >  2:914132/1‑99 (MQ=255)
    cGCTTCAACTTTGATGAACGGCGCATTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATAttttcg                                                                                            <  2:367364/100‑3 (MQ=255)
     gCTTCAACTTTGATGAACGGCGCATTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATAttttcgg                                                                                           <  2:2201085/100‑4 (MQ=255)
     gCTTCAACTTTGATGAACGGCGCATTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATAttttcgg                                                                                           <  2:1996802/100‑4 (MQ=255)
     gCTTCAACTTTGATGAACGGCGCATTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATAttttcgg                                                                                           <  2:1277721/100‑4 (MQ=255)
      cTTCAACTTTGATGAACGGCGCATTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATAttttcggg                                                                                          <  2:784537/100‑5 (MQ=255)
      cTTCAACTTTGATGAACGGCGCATTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGAGCCGATCATCAGGATAttttcggg                                                                                          >  1:132987/1‑96 (MQ=255)
       ttCAACTTTGTTGAACGGCGCATTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACTCCTGTCGGGCCGATCATCAGGATA‑TTTTCgggg                                                                                        <  1:1906129/100‑1 (MQ=255)
       ttCAACTTTGATGAACGGCGCATTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATA‑TTTTCgggg                                                                                        <  1:989173/100‑1 (MQ=255)
         cAACTTTGATGAACGGCGCATTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATA‑TTTTCGGGGTc                                                                                      <  2:753770/100‑1 (MQ=255)
         cAACTTTGATGAACGGCGCATTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATA‑TTTTCGGGGTc                                                                                      >  1:1876963/1‑100 (MQ=255)
           aCTTTGATGAACGGCGCATTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATA‑TTTTCGGGGTCAc                                                                                    >  2:2237144/1‑100 (MQ=255)
             tttGATGAACGGCGCATTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATA‑TTTTCGGGGTCACtt                                                                                  <  1:1464142/100‑1 (MQ=255)
                 atgaACGGCGCATTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATA‑TTTTCGGGGTCACTTCATg                                                                              >  1:2072094/1‑100 (MQ=255)
                  tgaACGGCGCATTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATA‑TTTTCGGGGTCACTTCATgg                                                                             <  1:1926643/100‑1 (MQ=255)
                  tgaACGGCGCATTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATA‑TTTTCGGGGTCACTTCATgg                                                                             <  1:1369487/100‑1 (MQ=255)
                   gaACGGCGCATTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATA‑TTTTCGGGGTCACTTCATGgc                                                                            >  2:2806501/1‑100 (MQ=255)
                   gaACGGCGCATTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATA‑TTTTCGGGGTCACTTCATGgc                                                                            >  2:391015/1‑100 (MQ=255)
                      cGGCGCATTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATA‑TTTTCGGGGTCACTTCATGgcgcn                                                                         <  1:1726753/100‑2 (MQ=255)
                      cGGCGCATTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATA‑TTTTCGGGGTCACTTCATGGCGCa                                                                         >  1:877736/1‑100 (MQ=255)
                      cGGCGCATTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATA‑TTTTCGGGGTCACTTCATGGCGCa                                                                         <  2:1689029/100‑1 (MQ=255)
                       ggCGCATTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATA‑TTTTCGGGGTCACTTCATGGCGCAg                                                                        >  2:603505/1‑100 (MQ=255)
                       ggCGCATTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATA‑TTTTCGGGGTCACTTCATGGCGCAg                                                                        >  2:1458830/1‑100 (MQ=255)
                        gcgcATTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATA‑TTTTCGGGGTCACTTCATGGCGCAGc                                                                       >  2:2408925/1‑100 (MQ=255)
                         cgcATTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATA‑TTTTCGGGGTCACTTCATGGCGCAGct                                                                      >  1:2859047/1‑100 (MQ=255)
                         cgcATTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATA‑TTTTCGGGGTCACTTCATGGCGCAGct                                                                      >  1:2859334/1‑100 (MQ=255)
                           cATTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATA‑TTTTCGGGGTCACTTCATGGCGCAGctct                                                                    >  2:1152298/1‑100 (MQ=255)
                           cATTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATA‑TTTTCGGGGTCACTTCATGGCGCAGctct                                                                    >  1:2860437/1‑100 (MQ=255)
                              tCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATA‑TTTTCGGGGTCACTTCATGGCGCAGCTCTTCg                                                                 <  1:2626184/100‑1 (MQ=255)
                               cGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATA‑TTTTCGGGGTCACTTCATGGCGCAGCTCTTCGt                                                                >  2:2480599/1‑100 (MQ=255)
                               cGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATA‑TTTTCGGGGTCACTTCATGGCGCAGCTCTTCGt                                                                <  2:1201454/100‑1 (MQ=255)
                               cGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATA‑TTTTCGGGGTCACTTCATGGCGCAGCTCTTCGt                                                                >  1:2827243/1‑100 (MQ=255)
                               cGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGACCATCAGGATA‑TTTTCGGGGTCACTTCATGGCGCAGCTCTTCGt                                                                >  1:957886/1‑100 (MQ=255)
                                gCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATA‑TTTTCGGGGTCACTTCATGGCGCAGCTCTTCGtt                                                               <  2:2023353/100‑1 (MQ=255)
                                gCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATA‑TTTTCGGGGTCACTTCATGGCGCAGCTCTTCGtt                                                               >  1:1840707/1‑100 (MQ=255)
                                    gCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGGTCATCAGGATA‑TTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTTAGc                                                           >  1:2631036/1‑100 (MQ=255)
                                    gCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATA‑TTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGc                                                           <  1:2855875/100‑1 (MQ=255)
                                    gCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATA‑TTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGc                                                           <  2:1581942/100‑1 (MQ=255)
                                     cTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATATTTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGc                                                           >  2:1851255/1‑100 (MQ=255)
                                     cTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATA‑TTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCt                                                          >  1:2182787/1‑100 (MQ=255)
                                       tAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATA‑TTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGc                                                        <  2:904177/100‑1 (MQ=255)
                                        aGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATA‑TTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCa                                                       >  2:199598/1‑100 (MQ=255)
                                         gCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATA‑TTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCAt                                                      >  2:2568394/1‑100 (MQ=255)
                                          ccAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATA‑TTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATg                                                     <  2:1434862/100‑1 (MQ=255)
                                            aGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATA‑TTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCg                                                   <  2:1208137/100‑1 (MQ=255)
                                              acgacgGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATA‑TTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGAc                                                 <  1:1093547/100‑1 (MQ=255)
                                               cgacgGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATA‑TTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACg                                                >  1:1120758/1‑100 (MQ=255)
                                               cgacgGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATA‑TTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACg                                                <  2:581654/100‑1 (MQ=255)
                                                  cgGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATA‑TTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCa                                             >  2:1824829/1‑100 (MQ=255)
                                                  cgGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATA‑TTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCa                                             <  2:1324287/100‑1 (MQ=255)
                                                   gggCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATA‑TTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAg                                            <  2:2346503/100‑1 (MQ=255)
                                                   gggCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATA‑TTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAg                                            >  1:2580644/1‑100 (MQ=255)
                                                     gCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATA‑TTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCg                                          <  1:1427949/100‑1 (MQ=255)
                                                      cGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATA‑TTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCgg                                         <  1:2257113/100‑1 (MQ=255)
                                                      cGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATA‑TTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCgg                                         <  1:1765946/100‑1 (MQ=255)
                                                      cGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATA‑TTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCgg                                         >  2:1882265/1‑100 (MQ=255)
                                                      cGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATA‑TTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCgg                                         <  2:2180915/100‑1 (MQ=255)
                                                       gATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATA‑TTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGt                                        <  1:1506807/100‑1 (MQ=255)
                                                        aTTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATA‑TTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGtt                                       <  2:1599917/100‑1 (MQ=255)
                                                         tttCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATA‑TTTTCGGGGTCACTTCCTGGCGCAGCTCTTCGTTTAGCTGCATGCGACGCCAGCGGTTa                                      >  1:2031077/1‑100 (MQ=255)
                                                         tttCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATA‑TTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTa                                      <  1:2775880/100‑1 (MQ=255)
                                                         tttCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATA‑TTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTa                                      >  2:2277746/1‑100 (MQ=255)
                                                           tCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATA‑TTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACg                                    <  2:463101/100‑1 (MQ=255)
                                                            cAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATA‑TTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGc                                   >  2:2073508/1‑100 (MQ=255)
                                                            cAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATA‑TTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGc                                   <  2:1229797/100‑1 (MQ=255)
                                                            cAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATA‑TTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGc                                   >  1:2202246/1‑100 (MQ=255)
                                                            cAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATA‑TTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGc                                   >  1:1262648/1‑100 (MQ=255)
                                                             aGTTTTACCGACACCGGTCGGGCCGATCATCAGGATA‑TTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCa                                  >  1:1721102/1‑100 (MQ=255)
                                                             aGTTTTACCGACACCGGTCGGGCCGATCATCAGGATA‑TTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCa                                  <  2:1120776/100‑1 (MQ=255)
                                                              gTTTTACCGACACCGGTCGGGCCGATCATCAGGATA‑TTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCag                                 <  2:639621/100‑1 (MQ=255)
                                                              gTTTTACCGACACCGGTCGGGCCGATCATCAGGAT‑GTTTTCGGGGTCACTTCATTGCGCAGCTCTTCGTTGAGCTGCATCCCACGCCAGCGGTTACGCag                                 >  2:1792253/1‑100 (MQ=255)
                                                               ttttACCGACACCGGTCGGGCCGATCATCAGGATA‑TTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCaga                                <  1:254588/100‑1 (MQ=255)
                                                                tttACCGACACCGGTCGGGCCGATCATCAGGATA‑TTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCagag                               <  2:1374813/100‑1 (MQ=255)
                                                                tttACCGACACCGGTCGGGCCGATCATCAGGATA‑TTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCagag                               <  1:935985/100‑1 (MQ=255)
                                                                tttACCGACACCGGTCGGGCCGATCATCAGGATA‑TTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCagag                               <  1:796765/100‑1 (MQ=255)
                                                                  tACCGACACCGGTCGGGCCGATCATCAGGATA‑TTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTCGGCTGCATGCGACGCACGCGGTTACACAGCGCa                             >  1:2222895/1‑100 (MQ=255)
                                                                   aCCGACACCGGTCGGGCCGATCATCAGGATA‑TTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCaa                            >  2:1393593/1‑100 (MQ=255)
                                                                    ccGACACCGGTCGGGCCGATCATCAGGATA‑TTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAAt                           >  2:2948/1‑100 (MQ=255)
                                                                      gACACCGGTCGGGCCGATCATCAGGATA‑TTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCg                         <  2:1721128/100‑1 (MQ=255)
                                                                      gACACCGGTCGGGCCGATCATCAGGATA‑TTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCg                         >  2:1020199/1‑100 (MQ=255)
                                                                      gACACCGGTCGGGCCGATCATCAGGATA‑TTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCg                         >  1:477341/1‑100 (MQ=255)
                                                                          ccGGTCGGGCCGATCATCAGGATA‑TTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCcac                     <  2:1429439/100‑1 (MQ=255)
                                                                           cGGTCGGGCCGATCATCAGGATA‑TTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCcaca                    <  2:958690/100‑1 (MQ=255)
                                                                           cGGTCGGGCCGATCATCAGGATA‑TTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCcaca                    <  1:1875339/100‑1 (MQ=255)
                                                                           cGGTCGGGCCGATCATCAGGATA‑TTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCcaca                    >  1:2558952/1‑100 (MQ=255)
                                                                           cGGTCGGGCCGATCATCAGGATA‑TTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCcaca                    >  2:878641/1‑100 (MQ=255)
                                                                           cGGTCGGGCCGATCATCAGGATA‑TTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCcaca                    <  2:1180319/100‑1 (MQ=255)
                                                                                gggCCGATCATCAGGATA‑TTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACg               >  2:1149248/1‑100 (MQ=255)
                                                                                 ggCCGATCATCAGGATA‑TTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGc              >  2:405402/1‑100 (MQ=255)
                                                                                     gATCATCAGGATA‑TTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTgg          <  1:776958/100‑1 (MQ=255)
                                                                                        catcaGGATA‑TTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAGCGCTTGGCGt       >  2:1738148/1‑100 (MQ=255)
                                                                                        catcaGGATA‑TTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGt       >  1:1184671/1‑100 (MQ=255)
                                                                                         atcaGGATA‑TTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGtt      >  2:2190566/1‑100 (MQ=255)
                                                                                          tcaGGATA‑TTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTg     <  2:442496/100‑1 (MQ=255)
                                                                                          tcaGGATA‑TTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTg     <  1:73766/100‑1 (MQ=255)
                                                                                             ggATA‑TTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTcc  >  1:696602/1‑100 (MQ=255)
                                                                                             ggATA‑TTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTcc  >  2:251823/1‑100 (MQ=255)
                                                                                                  |                                                                                               
TGGTCGCTTCAACTTTGATGAACGGCGCATTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATATTTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTCC  >  NC_000913/4121495‑4121688

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A