Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F110 I1 R1 51 90.5 4460762 96.5% 4304635 99.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,121,597 (T)5→4 coding (151/1332 nt) hslU ← molecular chaperone and ATPase component of HslUV protease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,121,5930T.97.3% 322.8 / 2.6 73coding (155/1332 nt)hslUmolecular chaperone and ATPase component of HslUV protease
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base . (41/30);  total (42/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.21e-01

TTGGTCGCTTCAACTTTGATGAACGGCGCATTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATATTTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTC  >  NC_000913/4121494‑4121687
                                                                                                   |                                                                                              
ttGGTCGCTTCAACTTTGATGAACGGCGCATTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGatat                                                                                                <  1:1844366/100‑1 (MQ=255)
ttGGTCGCTTCAACTTTGATGAACGGCGCATTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGatat                                                                                                <  1:1843694/100‑1 (MQ=255)
ttGGTCGCTTCAACTTTGATGAACGGCGCATTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGatat                                                                                                <  2:1169183/100‑1 (MQ=255)
ttGGTCGCTTCAACTTTGATGAACGGCGCATTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGatat                                                                                                <  1:726724/100‑1 (MQ=255)
 tGGTCGCTTCAACTTTGATGAACGGCGCATTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATAtt                                                                                               >  2:1164191/1‑100 (MQ=255)
  ggTCGCTTCAACTTTGATGAACGGCGCATTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATAttt                                                                                              >  1:210637/1‑100 (MQ=255)
   gTCGCTTCAACTTTGATGAACGGCGCATTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATAtttt                                                                                             >  1:732113/1‑100 (MQ=255)
   gTCGCTTCAACTTTGATGAACGGCGCATTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATAtttt                                                                                             >  2:680390/1‑100 (MQ=255)
     cGCTTCAACTTTGATGAACGGCGCATTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATAttttcg                                                                                           <  2:1187188/100‑3 (MQ=255)
       cTTCAACTTTGATGAACGGCGCATTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATAttttcggg                                                                                         <  2:76963/100‑5 (MQ=255)
       cTTCAACTTTGATGAACGGCGCATTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATAttttcggg                                                                                         <  2:1739551/100‑5 (MQ=255)
       cTTCAACTTTGATGAACGGCGCATTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATAttttcggg                                                                                         <  1:1852076/100‑5 (MQ=255)
        ttCAACTTTGATGAACGGCGCATTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATA‑TTTTCgggg                                                                                       <  2:277733/100‑1 (MQ=255)
        ttCAACTTTGATGAACGGCGCATTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATA‑TTTTCgggg                                                                                       <  2:723004/100‑1 (MQ=255)
        ttCAACTTTGATGAACGGCGCATTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATA‑TTTTCgggg                                                                                       <  1:1908723/100‑1 (MQ=255)
        ttCAACTTTGATGAACGGCGCATTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATA‑TTTTCgggg                                                                                       >  2:1553662/1‑100 (MQ=255)
          cAACTTTGATGAACGGCGCATTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATA‑TTTTCGGGGTc                                                                                     >  1:579043/1‑100 (MQ=255)
           aaCTTTGATGAACGGCGCATTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATA‑TTTTCGGGGTCa                                                                                    <  2:548429/100‑1 (MQ=255)
            aCTTTGATGAACGGCGCATTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATA‑TTTTCGGGGTCAc                                                                                   <  1:1221384/100‑1 (MQ=255)
            aCTTTGATGAACGGCGCATTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATA‑TTTTCGGGGTCAc                                                                                   <  2:154168/100‑1 (MQ=255)
             cTTTGATGAACGGCGCATTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGACGATCCTACCGATA‑TATTCGGGGTCAc                                                                                   >  2:740824/1‑99 (MQ=255)
                tgatgaACGGCGCATTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATA‑TTTTCGGGGTCACTTCa                                                                               >  2:206638/1‑100 (MQ=255)
                  atgaACGGCGCATTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATA‑TTTTCGGGGTCACTTCATg                                                                             >  2:293842/1‑100 (MQ=255)
                   tgaACTGCGCATTCGCCAGCTTAGCCAGACGACGTGCGATTTCAGTTGTACCGACACCGGTCGGGCCGATCATCAGGATA‑TTTTCGGGGTCACTTCATgg                                                                            <  1:903004/100‑1 (MQ=255)
                   tgaACGGCGCATTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATA‑TTTTCGGGGTCACTTCATgg                                                                            >  1:350584/1‑100 (MQ=255)
                   tgaACGGCGCATTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATA‑TTTTCGGGGTCACTTCATgg                                                                            <  2:1895263/100‑1 (MQ=255)
                   tgaACGGCGCATTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATA‑TTTTCGGGGTCACTTCATgg                                                                            >  1:1706255/1‑100 (MQ=255)
                    gaACGGCGCATTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATA‑TTTTCGGGGTCACTTCATGgc                                                                           <  1:1576364/100‑1 (MQ=255)
                     agcGGCGCATTCGCCAGCTTAGCCAGACGACGGGCGATTGCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATA‑TTTTCGGGGTCACTTCATGgc                                                                           <  1:545840/97‑1 (MQ=255)
                       cGGCGCATTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATA‑TTTTCGGGGTCACTTCATGGCGCa                                                                        <  2:66896/100‑1 (MQ=255)
                       cGGCGCATTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATA‑TTTTCGGGGTCACTTCATGGCGCa                                                                        <  2:38853/100‑1 (MQ=255)
                        ggCGCATTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATA‑TTTTCGGGGTCACTTCATGGCGCAg                                                                       >  2:1224474/1‑100 (MQ=255)
                            cATTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATA‑TTTTCGGGGTCACTTCATGGCGCAGctct                                                                   >  1:1732889/1‑100 (MQ=255)
                             nttCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATA‑TTTTCGGGGTCACTTCATGGCGCAGCTCtt                                                                  >  2:2038791/2‑100 (MQ=255)
                               tCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATA‑TTTTCGGGGTCACTTCATGGCGCAGCTCTTc                                                                 >  2:553129/1‑99 (MQ=255)
                                cGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATA‑TTTTCGGGGTCACTTCATGGCGCAGCTCTTCGt                                                               >  1:1980850/1‑100 (MQ=255)
                                cGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATA‑TTTTCGGGGTCACTTCATGGCGCAGCTCTTCGt                                                               >  2:741355/1‑100 (MQ=255)
                                cGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATA‑TTTTCGGGGTCACTTCATGGCGCAGCTCTTCGt                                                               >  1:800737/1‑100 (MQ=255)
                                cGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATA‑TTTTCGGGGTCACTTCATGGCGCAGCTCTTCGt                                                               >  1:1980900/1‑100 (MQ=255)
                                 gCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATA‑TTTTCGGGGTCACTTCATGGCGCAGCTCTTCGtt                                                              >  1:699232/1‑100 (MQ=255)
                                  ccAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATA‑TTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTg                                                             <  2:1330017/100‑1 (MQ=255)
                                    aGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATA‑TTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAg                                                           >  1:1279954/1‑100 (MQ=255)
                                     gCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATA‑TTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGc                                                          >  2:2220760/1‑100 (MQ=255)
                                      cTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATA‑TTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCt                                                         >  2:1090124/1‑100 (MQ=255)
                                       ttAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATA‑TTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTg                                                        <  2:1728731/100‑1 (MQ=255)
                                         aGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATATTTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGc                                                       >  1:135458/1‑100 (MQ=255)
                                           ccAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATA‑TTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATg                                                    <  2:960224/100‑1 (MQ=255)
                                           ccAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATA‑TTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATg                                                    <  1:1257109/100‑1 (MQ=255)
                                            cAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATA‑TTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGc                                                   <  1:647538/100‑1 (MQ=255)
                                            cAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATA‑TTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGc                                                   >  1:1912939/1‑100 (MQ=255)
                                             aGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATA‑TTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCg                                                  >  1:440533/1‑100 (MQ=255)
                                              gacgacGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATA‑TTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGa                                                 >  1:828239/1‑100 (MQ=255)
                                               acgacgGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATA‑TTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGAc                                                >  1:200723/1‑100 (MQ=255)
                                                cgacgGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATA‑TTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGAc                                                >  1:432493/1‑99 (MQ=255)
                                                cgacgGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATA‑‑TTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGc                                              >  1:1212105/1‑100 (MQ=255)
                                                  acgGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATA‑TTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGcc                                             <  1:2220226/100‑1 (MQ=255)
                                                     ggCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATA‑TTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGc                                          >  1:304107/1‑100 (MQ=255)
                                                          tttCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATA‑TTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTa                                     >  1:368974/1‑100 (MQ=255)
                                                          tttCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATA‑TTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTa                                     <  2:1160104/100‑1 (MQ=255)
                                                           ttCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATA‑TTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTAc                                    >  1:1398940/1‑100 (MQ=255)
                                                              aGTTTTACCGACACCGGTCGGGCCGATCATCAGGATA‑TTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCa                                 <  1:755924/100‑1 (MQ=255)
                                                              aGTTTTACCGACACCGGTCGGGCCGATCATCAGGATA‑TTCTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCa                                 >  1:574156/1‑100 (MQ=255)
                                                               gTTTTACCGACACCGGTCGGGCCGATCATCAGGATA‑TTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCag                                >  1:7864/1‑100 (MQ=255)
                                                                   tACCGACACCGGTCGGGCCGATCATCAGGATA‑TGTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCGGCATGCGACGCCAGCGGTTACGCAGAGCa                            >  1:1334811/1‑100 (MQ=255)
                                                                     ccGACACCGGTCGGGCCGATCATCAGGATA‑TTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAAt                          >  2:420352/1‑100 (MQ=255)
                                                                       gACACCGGTCGGGCCGATCATCAGGATA‑TTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCg                        <  2:1033819/100‑1 (MQ=255)
                                                                       gACACCGGTCGGGCCGATCATCAGGATA‑TCTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCg                        <  1:1339303/100‑1 (MQ=255)
                                                                        acacCGGTCGGGCCGATCATCAGGATA‑TTGTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGc                       <  1:1340458/100‑1 (MQ=255)
                                                                         cacCGGTCGGGCCGATCATCAGGATA‑TTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGcc                      >  2:1023693/1‑100 (MQ=255)
                                                                           ccGGTCGGGCCGATCATCAGGATA‑TTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCcac                    >  2:1266315/1‑100 (MQ=255)
                                                                           ccGGTCGGGCCGATCATCAGGATA‑TTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCcac                    <  2:200723/100‑1 (MQ=255)
                                                                            cGGTCGGGCCGATCATCAGGATA‑TTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCcaca                   >  2:388340/1‑100 (MQ=255)
                                                                            cGGTCGGGCCGATCATCAGGATA‑TTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCcaca                   <  2:1279956/100‑1 (MQ=255)
                                                                            cGGTCGGGCCGATCATCAGGATA‑TTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCcaca                   <  2:1114057/100‑1 (MQ=255)
                                                                                 gggCCGATCATCAGGATA‑TTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACg              >  2:1999065/1‑100 (MQ=255)
                                                                                  ggCCGATCATCAGGATA‑TTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGc             >  2:1154529/1‑100 (MQ=255)
                                                                                  ggCCGATCATCAGGATA‑TTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGc             >  2:689348/1‑100 (MQ=255)
                                                                                      gATCATCAGGATA‑TTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTgg         >  1:2157357/1‑100 (MQ=255)
                                                                                      gATCATCAGGATA‑‑TTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGc        <  2:1212107/100‑1 (MQ=255)
                                                                                       atcatcAGGATA‑TTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGc        <  2:1990778/100‑1 (MQ=255)
                                                                                        tcatcaGGATA‑TTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCg       <  2:936292/100‑1 (MQ=255)
                                                                                         catcaGGATA‑TTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGt      <  1:1136714/100‑1 (MQ=255)
                                                                                           tcaGGATA‑TTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTg    >  2:1775000/1‑100 (MQ=255)
                                                                                             aGGATATTTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGt   <  2:984569/100‑1 (MQ=255)
                                                                                             aGGATA‑TTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTc  >  2:6468/1‑100 (MQ=255)
                                                                                                   |                                                                                              
TTGGTCGCTTCAACTTTGATGAACGGCGCATTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATATTTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTC  >  NC_000913/4121494‑4121687

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A