Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A16 F258 I1 R1 132 116.1 5785507 94.2% 5449947 99.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,645,196 G→A intergenic (‑14/‑189) prlC ← / → rlmJ oligopeptidase A/23S rRNA m(6)A2030 methyltransferase, SAM‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,645,1960GA99.2% 439.5 / ‑4.6 128intergenic (‑14/‑189)prlC/rlmJoligopeptidase A/23S rRNA m(6)A2030 methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (68/59);  total (68/60)
Fisher's exact test for biased strand distribution p-value = 4.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.20e-01

Only 100 of 129 total aligned reads displayed.

GCCTTAGTCACGGCTGGAACGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTCAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCAGTCCTGGTTAAAGAGGTTAAGGAAGCGCTCAACAGGCGCTTTACATAATGTGTGTAGCATGGGGTTAAGTGTAGTGAATTTCAATGAGAAACGTTACGCTTTC  >  NC_000913/3645097‑3645292
                                                                                                   |                                                                                                
  cTTAGTCACGGCTGGAACGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTCAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCAGTCCTGAtt                                                                                                <  1:555339/100‑1 (MQ=255)
   ttAGTCACGGCTGGAACGACATGTTCCTGGAGAATTTTAGAAAACGGAGGCAATTCAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCAGTCCTGAtt                                                                                                <  1:1509732/99‑1 (MQ=255)
    tAGTCACGGCTGGAACGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTCAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCAGTCCTGATTaa                                                                                              <  1:2715642/100‑1 (MQ=255)
    tAGTCACGGCTGGAACGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTCAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCAGTCCTGATTaa                                                                                              >  2:2451378/1‑100 (MQ=255)
    tAGTCACGGCTGGAACGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTCAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCAGTCCTGATTa                                                                                               >  1:375307/1‑99 (MQ=255)
     aGTCACGGCTGGAACGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTCAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCAGTCCTGATTaaa                                                                                             >  1:41311/1‑100 (MQ=255)
     aGTCACGGCTGGAACGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTCAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCAGTCCTGATTaaa                                                                                             >  1:500991/1‑100 (MQ=255)
      gTCACGGCTGGAACGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTCAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCAGTCCTGATTAAag                                                                                            >  2:958017/1‑100 (MQ=255)
      gTCACGGCTGGAACGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTCAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCAGTCCTGATTAAag                                                                                            <  1:2465050/100‑1 (MQ=255)
      gTCACGGCTGGAACGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTCAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCAGTCCTGATTAAag                                                                                            <  1:2090897/100‑1 (MQ=255)
       tCACGGCTGGAACGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTCAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCAGTCCTGATTAAaga                                                                                           <  2:553134/100‑1 (MQ=255)
       tCACGGCTGGAACGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTCAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCAGTCCTGATTAAaga                                                                                           <  2:884646/100‑1 (MQ=255)
        cACGGCTGGAACGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTCAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCAGTCCTGATTAAagag                                                                                          >  1:756897/1‑100 (MQ=255)
        cACGGCTGGAACGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTCAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCAGTCCTGATTAAagag                                                                                          >  2:911939/1‑100 (MQ=255)
        cACGGCTGGAACGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTCAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCAGTCCTGATTAAagag                                                                                          <  2:2284942/100‑1 (MQ=255)
        cACGGCTGGAACGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTCAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCAGTCCTGATTAAagag                                                                                          >  2:110346/1‑100 (MQ=255)
        cACGGCTGGAACGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTCAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCAGTCCTGATTAAagag                                                                                          >  2:1380802/1‑100 (MQ=255)
            gCTGGAACGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTCAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCAGTCCTGATTAAAGAGGTTa                                                                                      <  2:2141190/100‑1 (MQ=255)
               ggAACGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTCAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCAGTCCTGATTAAAGAGGTTAAgg                                                                                   >  2:432881/1‑100 (MQ=255)
                gAACGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCACTTCAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCAGTCCTGATTAAAGAGGTTAAGGa                                                                                  <  2:658003/100‑1 (MQ=255)
                gAACGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTCAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCAGTCCTGATTAAAGAGGTTAAg                                                                                    >  2:2573766/1‑98 (MQ=255)
                 aaCGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTCAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCAGTCCTGATTAAAGAGGTTAAGGaa                                                                                 >  2:2741584/1‑100 (MQ=255)
                  aCGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTCAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCAGTCCTGATTAAAGAGGTTAAGGAAg                                                                                <  2:2174892/100‑1 (MQ=255)
                  aCGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTCAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCAGTCCTGATTAAAGAGGTTAAGGAAg                                                                                <  2:1248904/100‑1 (MQ=255)
                     aCATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTCAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCAGTCCTGATTAAAGAGGTTAAGGAAgcgn                                                                             <  2:2453971/100‑2 (MQ=255)
                      cATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTCAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCAGTCCTGATTAAAGAGGTTAAGGAAGCGCt                                                                            >  1:573873/1‑100 (MQ=255)
                        tcttCCGGGAGAATTTTAGAAAACGGAGGCAATTCAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCAGTCCTGATTAAAGAGGTTAAGGAAGCGCTCa                                                                          <  2:1755623/98‑1 (MQ=255)
                        tGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTCAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCAGTCCTGATTAAAGAGGTTAAGGAAGCGCTCa                                                                          >  2:195044/1‑100 (MQ=255)
                        tGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTCAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCAGTCCTGATTAAAGAGGTTAAGGAAGCGCTCa                                                                          >  2:1466543/1‑100 (MQ=255)
                        tGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTCAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCAGTCCTGATTAAAGAGGTTAAGGAAGCGCTCa                                                                          <  2:411769/100‑1 (MQ=255)
                           tCCGGGAGAATTTTAGAAAACGGAGGCAATTCAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCAGTCCTGATTAAAGAGGTTAAGGAAGCGCTCAACa                                                                       >  1:1172456/1‑100 (MQ=255)
                            ccGGGAGAATTTTAGAAAACGGAGGCAATTCAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCAGTCCTGATTAAAGAGGTTAAGGAAGCGCTCAACAg                                                                      <  2:41311/100‑1 (MQ=255)
                              gggAGAATTTTAGAAAACGGAGGCAATTCAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCAGTCCTGATTAAAGAGGTTAAGGAAGCGCTCAACAGgc                                                                    >  2:1895983/1‑100 (MQ=255)
                              gggAGAATTTTAGAAAACGGAGGCAATTCAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCAGTCCTGATTAAAGAGGTTAAGGAAGCGCTCAACAGgc                                                                    <  2:2355018/100‑1 (MQ=255)
                                gagaATTTTAGAAAACGGAGGCAATTCAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCAGTCCTGATTAAAGAGGTTAAGGAAGCGCTCAACAGgcgc                                                                  >  2:2488233/1‑100 (MQ=255)
                                    aTTTTAGTAAACGGAGGCAATTCAAAGGGAGTCAGTAACGGATTCCTCATTCGCGCAGTCCTGATTAAAGAGGTTAAGGAAGCGCTCAACAGGCGCTTTa                                                              <  1:1041002/100‑1 (MQ=255)
                                    aTTTTAGAAAACGGAGGCAATTCAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCAGTCCTGATTAAAGAGGTTAAGGAAGCGCTCAACAGGCGCTTTa                                                              >  1:2851140/1‑100 (MQ=255)
                                    aTTTTAGAAAACGGAGGCAATTCAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCAGTCCTGATTAAAGAGGTTAAGGAAGCGCTCAACAGGCGCTTTa                                                              <  1:1266199/100‑1 (MQ=255)
                                    aTTTTAGAAAACGGAGGCAATTCAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCAGTCCTGATTAAAGAGGTTAAGGAAGCGCTCAACAGGCGCTTTa                                                              >  1:1400546/1‑100 (MQ=255)
                                    aTTTTAGAAAACGGAGGCAATTCAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCAGTCCTGATTAAAGAGGTTAAGGAAGCGCTCAACAGGCGCTTTa                                                              >  1:950397/1‑100 (MQ=255)
                                    aTTTTAGAAAACGGAGGCAATTCAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCAGTCCTGATTAAAGAGGTTAAGGAAGCGCTCAACAGGCGCTTTa                                                              <  1:2522653/100‑1 (MQ=255)
                                     ttttAGAAAACGGAGGCAATTCAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCAGTCCTGATTAAAGAGGTTAAGGAAGCGCTCAACAGGCGCTTTAc                                                             >  2:409957/1‑100 (MQ=255)
                                      tttAGAAAACGGAGGCAATTCAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCAGTCCTGATTAAAGAGGTTAAGGAAGCGCTCAACAGGCGCTTTACa                                                            <  1:138019/100‑1 (MQ=255)
                                        tAGAAAACGGAGGCAATTCAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCAGTCCTGATTAAAGAGGTTAAGGAAGCGCTCAACAGGCGCTTTACATa                                                          >  2:1608452/1‑100 (MQ=255)
                                          gAAAACGGAGGCAATTCAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCAGTCCTGATTAAAGAGGTTAAGGAAGCGCTCAACAGGCGCTTTACATAAt                                                        >  2:1498655/1‑100 (MQ=255)
                                           aaaaCGGAGGCAATTCAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCAGTCCTGATTAAAGAGGTTAAGGAAGCGCTCAACAGGCGCTTTACATAAtg                                                       <  2:2294305/100‑1 (MQ=255)
                                             aaCGGAGGCAATTCAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCAGTCCTGATTAAAGAGGTTAAGGAAGCGCTCAACAGGCGCTTTACATAAtgtg                                                     <  2:2098743/100‑1 (MQ=255)
                                             aaCGGAGGCAATTCAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCAGTCCTGATTAAAGAGGTTAAGGAAGCGCTCAACAGGCGCTTTACATAAtgtg                                                     >  2:1618549/1‑100 (MQ=255)
                                             aaCGGAGGCAATTCAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCAGTCCTGATTAAAGAGGTTAAGGAAGCGCTCAACAGGCGCTTTACATAAtgtg                                                     <  1:2409680/100‑1 (MQ=255)
                                                ggAGGCAATTCAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCAGTCCTGATTAAAGAGGTTAAGGAAGCGCTCAACAGGCGCTTTACATAATgtgtgt                                                  <  1:2488249/100‑1 (MQ=255)
                                                 gAGGCAATTCAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCAGTCCTGATTAAAGAGGTTAAGGAAGCGCTCAACAGGCGCTTTACATAATGTGTGTa                                                 <  1:1033824/100‑1 (MQ=255)
                                                  aGGCAATTCAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCAGTCCTGATTAAAGAGGTTAAGGAAGCGCTCAACAGGCGCTTTACATAATGTGTGTAg                                                <  1:756662/100‑1 (MQ=255)
                                                  aGGCAATTCAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCAGTCCTGATTAAAGAGGTTAAGGAAGCGCTCAACAGGCGCTTTACATAATGTGTGTAg                                                >  1:34047/1‑100 (MQ=255)
                                                   ggCAATTCAAAGGGAGTCAGTAACGTATTCGTCATTCGCGCAGTCCTGATTAAAGAGGTTAAGGAAGCGCTCAACAGGCGCTTTACATAATGTGTGTAGc                                               <  2:2804033/100‑1 (MQ=255)
                                                    gCAATTCAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCAGTCCTGATTAAAGAGGTTAAGGAAGCGCTCAACAGGCGCTTTACATAATGTGTGTAGCa                                              >  2:1339871/1‑100 (MQ=255)
                                                         tCAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCAGTCCTGATTAAAGAGGTTAAGGAAGCGCTCAACAGGCGCTTTACATAATGTGTGTAGCATgggg                                         <  2:210425/100‑1 (MQ=255)
                                                         tCAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCAGTCCTGATTAAAGAGGTTAAGGAAGCGCTCAACAGGCGCTTTACATAATGTGTGTAGCATgggg                                         <  1:2694254/100‑1 (MQ=255)
                                                          cAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCAGTCCTGATTAAAGAGGTTAAGGAAGCGCTCTACAGGCGCTTTACATAATGTGTGTAGCATgggg                                         <  2:1883875/99‑1 (MQ=255)
                                                          cAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCAGTCCTGATTAAAGAGGTTAAGGAAGCGCTCAACAGGCGCTTTACATAATGTGTGTAGCATgggg                                         >  2:1148596/1‑99 (MQ=255)
                                                          cAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCAGTCCTGATTAAAGAGGTTAAGGAAGCGCTCAACAGGCGCTTTACATAATGTGTGTAGCATgggg                                         >  2:292060/1‑99 (MQ=255)
                                                          cAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCAGTCCTGATTAAAGAGGTTAAGGAAGCGCTCAACAGGCGCTTTACATAATGTGTGTAGCATGGGGt                                        >  1:2688047/1‑100 (MQ=255)
                                                          cAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCAGTCCTGATTAAAGAGGTTAAGGAAGCGCTCAACAGGCGCTTTACATAATGTGTGTAGCATGGGGt                                        >  1:2688180/1‑100 (MQ=255)
                                                          cAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCAGTCCTGATTAAAGAGGTTAAGGAAGCGCTCAACAGGCGCTTTACATAATGTGTGTAGCATGGGGt                                        >  1:1090371/1‑100 (MQ=255)
                                                          cAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCAGTCCTGATTAAAGAGGTTAAGGAAGCACTCAACAGGCGCTTTACATAATGTGTGTAGCATGGGGt                                        >  2:1575333/1‑100 (MQ=255)
                                                            cttccgatctgTAACGGATTCGTCATTCGCGCAGTCCTGATTAAAGAGGTTAAGGAAGCGCTCAACAGGCGCTTTACATAATGTGTGTAGCATGGGGTTa                                      <  1:806642/90‑1 (MQ=255)
                                                              gggAGTCAGTAACGGATTCGTCATTCGCGCAGTCCTGATTAAAGAGGTTAAGGAAGCGCTCAACAGGCGCTTTACATAATGTGTGTAGCATGGGGTTAAg                                    <  1:2647846/100‑1 (MQ=255)
                                                                 aGTCAGTAACGGATTCGTCATTCGCGCAGTCCTGATTAAAGAGGTTAAGGAAGCGCTCAACAGGCGCTTTACATAATGTGTGTAGCATGGGGTTAAgtgt                                 <  2:1466643/100‑1 (MQ=255)
                                                                  gTCAGTAACGGATTCGTCATTCGCGCAGTCCTGATTAAAGAGGTTAAGGAAGCGCTCAACAGGCGCTTTACATAATGTGTGTAGCATGGGGTTAAGTGTa                                >  2:1624791/1‑100 (MQ=255)
                                                                  gTCAGTAACGGATTCGGCATTCGCGCAGTCCTGATTAAAGAGGTTAAGGAAGCGCTCAACAGGCGCTTTACATAATGTGTGTAGCAGGGGGTTAAGTGTa                                >  2:581326/1‑100 (MQ=255)
                                                                    gatctaCGGATTCGTCATTCGCGCAGTCCTGATTAAAGAGGTTAAGGAAGCGCTCAACAGGCGCTTTACATAATGTGTGTAGCATGGGGTTAAGTGTAGt                              <  1:1484694/95‑1 (MQ=255)
                                                                    cAGTAACGGATTCGTCATTCGCGCAGTCCTGATTAAAGAGGTTAAGGAAGCGCTCAACAGGCGCTTTACATAATGTGTGTAGCATGGGGTTAAGTGTAg                               >  1:748100/1‑99 (MQ=255)
                                                                    cAGTAACGGATTCGTCATTCGCGCAGTCCTGATTAAAGAGGTTAAGGAAGCGCTCAACAGGCGCTTTACATAATGTGTGTAGCATGGGGTTAAGTGTAGt                              <  2:332039/100‑1 (MQ=255)
                                                                    cAGTAACGGATTCGTCATTCGCGCAGTCCTGATTAAAGAGGTTAAGGAAGCGCTCAACAGGCGCTTTACATAATGTGTGTAGCATGGGGTTAAGTGTAGt                              >  2:286061/1‑100 (MQ=255)
                                                                    cAGTAACGGATTCGTCATTCGCGCAGTCCTGATTAAAGAGGTTAAGGAAGCGCTCAACAGGCGCTTTACATAATGTGTGTAGCATGGGGTTAAGTGTAGt                              <  2:2613899/100‑1 (MQ=255)
                                                                    cAGTAACGGATTCGTCATTCGCGCAGTCCTGATTAAAGAGGTTAAGGAAGCGCTCAACAGGCGCTTTACATAATGTGTGTAGCATAGGGTTAAGTGTAGt                              >  2:1040433/1‑100 (MQ=255)
                                                                      gTAACGGATTCGTCATTCGCGCAGTCCTGATTAAAGAGGTTAAGGAAGCGCTCAACAGGCGCTTTACATAATGTGTGTAGCATGGGGTTAAgatcggaag                            >  2:806641/1‑92 (MQ=255)
                                                                      gTAACGGATTCGTCATTCGCGCAGTCCTGATTAAAGAGGTTAAGGAAGCGCTCAACAGGCGCTTTACATAATGTGTGTAGCATGGGGTTAAGTGTAGTGa                            >  2:1916967/1‑100 (MQ=255)
                                                                      gTAACGGATTCGTCATTCGCGCAGTCCTGATTAAAGAGGTTAAGGAAGCGCTCAACAGGCGCTTTACATAATGTGTGTAGCATGGGGTTAAGTGTAGTGa                            >  2:972524/1‑100 (MQ=255)
                                                                      gTAACGGATTCGTCATTCGCGCAGTCCTGATTAAAGAGGTTAAGGAAGCGCTCAACAGGCGCTTTACATAATGTGTGTAGCATGGGGTTAAGTGTAGTGa                            >  2:1856800/1‑100 (MQ=255)
                                                                      gTAACGGATTCGTCATTCGCGCAGTCCTGATTAAAGAGGTTAAGGAAGCGCTCAACAGGCGCTTTACATAATGTGTGTAGCATGGGGTTAAGTGTAGTGa                            >  2:138050/1‑100 (MQ=255)
                                                                       tAACGGATTCGTCATTCGCGCAGTCCTGATTAAAGAGGTTAAGGAAGCGCTCAACAGGCGCTTTACATAATGTGTGTAGCATGGGGTTAAGTGTAGTGaa                           <  2:1003140/100‑1 (MQ=255)
                                                                       tAACGGATTCGTCATTCGCGCAGTCCTGATTAAAGAGGTTAAGGAAGCGCTCAACAGGCGCTTTACATAATGTGTGTAGCATGGGGTTAAGTGTAGTGaa                           <  1:2062350/100‑1 (MQ=255)
                                                                        aaCGGATTCGTCATTCGCGCAGTCCTGATTAAAGAGGTTAAGGAAGCGCTCAACAGGCGCTTTACATAATGTGTGTAGCATGGGGTTAAGTGTAGTGAAt                          <  1:1466550/100‑1 (MQ=255)
                                                                         aCGGATTCGTCATTCGCGCAGTCCTGATTAAAGAGGTTAAGGAAGCGCTCAACAGGCGCTTTACATAATGTGTGTAGCATGGGGTTAAGTGTAGTGAAtt                         <  2:1483484/100‑1 (MQ=255)
                                                                         aCGGATTCGTCATTCGCGCAGTCCTGATTAAAGAGGTTAAGGAAGCGCTCAACAGGCGCTTTACATAATGTGTGTAGCATGGGGTTAAGTGTAGTGAAtt                         <  2:1543276/100‑1 (MQ=255)
                                                                          cGGATTCGTCATTCGCGCAGTCCTGATTAAAGAGGTTAAGGAAGCGCTCAACAGGCGCTTTACATAATGTGTGTAGCATGGGGTTAAGTGTAGTGAAttt                        >  2:2708341/1‑100 (MQ=255)
                                                                             aTTCGTCATTCGCGCAGTCCTGATTAAAGAGGTTAAGGAAGCGCTCAACAGGCGCTTTACATAATGTGTGTAGCATGGGGTTAAGTGTAGTGAATTTCaa                     <  1:972526/100‑1 (MQ=255)
                                                                             aTTCGTCATTCGCGCAGTCCTGATTAAAGAGGTTAAGGAAGCGCTCAACAGGCGCTTTACATAATGTGTGTAGCATGGGGTTAAGTGTAGTGAATTTCaa                     <  1:909924/100‑1 (MQ=255)
                                                                              ttCGTCATTCGCGCAGTCCTGATTAAAGAGGTTAAGGAAGCGCTCAACAGGCGCTTTACATAATGTGTGTAGCATGGGGTTAAGTGTAGTGAATTTCAAt                    <  2:852102/100‑1 (MQ=255)
                                                                               tCGTCATTCGCGCAGTCCTGATTAAAGAGGTTAAGGAAGCGCTCAACAGGCGCTTTACATAATGTGTGTAGCATGGGGTTAAGTGTAGTGAATTTCAATg                   <  1:846913/100‑1 (MQ=255)
                                                                                cGTCATTCGCGCAGTCCTGATTAAAGAGGTTAAGGAAGCGCTCAACAGGCGCTTTACATAATGTGTGTAGCATGGGGTTAAGTGTAGTGAATTTCAATga                  >  1:2317241/1‑100 (MQ=255)
                                                                                cGTCATTCGCGCAGTCCTGATTAAAGAGGTTAAGGAAGCGCTCAACAGGCGCTTTACATAATGTGTGTAGCATGGGGTTAAGTGTAGTGAATTTCAATga                  >  1:133327/1‑100 (MQ=255)
                                                                                    aTTCGCGCAGTCCTGATTAAAGAGGTTAAGGAAGCGCTCAACAGGCGCTTTACATAATGTGTGTAGCATGGGGTTAAGTGTAGTGAATTTCAATGAGaaa              <  2:2280771/100‑1 (MQ=255)
                                                                                       cgcgCAGTCCTGATTAAAGAGGTTAAGGAAGCGCTCAACAGGCGCTTTACATAATGTGTGTAGCATGGGGTTAAGTGTAGTGAATTTCAATGAGAAACGt           >  2:1842333/1‑100 (MQ=255)
                                                                                        gcgcAGTCCTGATTAAAGAGGTTAAGGAAGCGCTCAACAGGCGCTTTACATAATGTGTGTAGCATGGGGTTAAGTGTAGTGAATTTCAATGAGAAACGtt          >  2:2569383/1‑100 (MQ=255)
                                                                                        gcgcAGTCCTGATTAAAGAGGTTAAGGAAGCGCTCAACAGGCGCTTTACATAATGTGTGTAGCATGGGGTTAAGTGTAGTGAATTTCAATGAGAAACGtt          >  2:1968222/1‑100 (MQ=255)
                                                                                        gcgcAGTCCTGATTAAAGAGGTTAAGGAAGCGCTCAACAGGCGCTTTACATAATGTGTGTAGCATGGGGTTAAGTGTAGTGAATTTCAATGAGAAACGtt          >  1:2026518/1‑100 (MQ=255)
                                                                                        gcgcAGTCCTGATTAAAGAGGTTAAGGAAGCGCTCAACAGGCGCTTTACATAATGTGTGTAGCATGGGGTTAAGTGTAGTGAATTTCAATGAGAAACGtt          >  2:2514656/1‑100 (MQ=255)
                                                                                               ccTGATTAAAGAGGTTAAGGAAGCGCTCAACAGGCGCTTTACATAATGTGTGTAGCATGGGGTTAAGTGTAGTGAATTTCAATGAGAAACGTTACGCttt   <  1:990883/100‑1 (MQ=255)
                                                                                                cTGATTAAAGAGGTTAAGGAAGCGCTCAACAGGCGCTTTACATAATGTGTGTAGCATGGGGTTAAGTGTAGTGAATTTCAATGAGAAACGTTACGCTTTc  >  1:2362691/1‑100 (MQ=255)
                                                                                                   |                                                                                                
GCCTTAGTCACGGCTGGAACGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTCAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCAGTCCTGGTTAAAGAGGTTAAGGAAGCGCTCAACAGGCGCTTTACATAATGTGTGTAGCATGGGGTTAAGTGTAGTGAATTTCAATGAGAAACGTTACGCTTTC  >  NC_000913/3645097‑3645292

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A