Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A16 F258 I1 R1	132	116.1	5785507	94.2%	5449947	99.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,742,218	A→G	A105A (GCT→GCC)	yiaJ ←	transcriptional repressor for the yiaKLMNO‑lyxK‑sgbHUE operon

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,742,218	0	A	G	99.0%	335.2 / NA	97	A105A (GCT→GCC)	yiaJ	transcriptional repressor for the yiaKLMNO‑lyxK‑sgbHUE operon
Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (57/38); total (57/40)
Fisher's exact test for biased strand distribution p-value = 4.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGAAGTTAATGGTTTCACCAGTGGCGATGTTCAGTGCCTCAAGATGCGGAGCGGCGATATGAATGATATTCAGCGAAGACAGCGCCTTCTGCCCGACGGCAATAAATTTGGTGGTCAGGCGATAACTCCCTGCGGCAGGCGCGGTG  >  NC_000913/3742124‑3742314

gTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGAAGTTAATGGTTTCACCAGTGGCGATGTTCAGTGCCTCAAGATGCGGggcggc                                                                                             <  2:430060/100‑1 (MQ=255)

gTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGAAGTTAATGGTTTCACCAGTGGCGATGTTCAGTGCCTCAAGATGCGGggcggc                                                                                             >  2:2459791/1‑100 (MQ=255)

   ccAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGAAGTTAATGGTTTCACCAGTGGCGATGTTCAGTGCCTCAAGATGCGGGGCGGCGat                                                                                          <  1:2099793/100‑1 (MQ=255)

   ccAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGAAGTTAATGGTTTCACCAGTGGCGATGTGCAGTGCCTCAAGATGCGGGGCGGCGat                                                                                          >  1:1305622/1‑100 (MQ=255)

    cAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGAAGTTAATGGTTTCACCAGTGGCGATGTTCAGTGCCTCAAGATGCGGGGCGGCGata                                                                                         >  1:338914/1‑100 (MQ=255)

    cAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGAAGTTAATGGTTTCACCAGTGGCGATGTTCAGTGCCTCAAGATGCGGGGCGGCGata                                                                                         >  2:1795526/1‑100 (MQ=255)

    cAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGAAGTTAATGGTTTCACCAGTGGCGATGTTCAGTGCCTCAAGATGCGGGGCGGCGata                                                                                         <  1:2069310/100‑1 (MQ=255)

     aGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGCAGTTAATGGTTTCACCAGTGGCGATGTTCAGTGCCTCAAGATGCGGGGCGGCGatat                                                                                        >  1:1964787/1‑100 (MQ=255)

     aGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGAAGTTAATGGTTTCACCAGTGGCGATGTTCAGTGCCTCAAGATGCGGGGCGGCGatat                                                                                        <  2:450042/100‑1 (MQ=255)

      gCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGAAGTTAATGGTTTCACCAGTGGCGATGTTCAGTGCCTCAAGCTTCGGGGCGGCGATatg                                                                                       >  2:1357667/1‑100 (MQ=255)

      gCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGAAATTAATGGTTTCACCAGTGGCGATGTTCAGTGCCTCAAGATGCGGGGCGGCGATatg                                                                                       <  2:1855329/100‑1 (MQ=255)

         tataAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGAAGTTAATGGTTTCACCAGTGGCGATGTTCAGTGCCTCAAGATGCGGGGCGGCGATatgaat                                                                                    <  1:1038877/100‑1 (MQ=255)

         tataAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGAAGTTAATGGTTTCACCAGTGGCGATGTTCAGTGCCTCAAGATGCGGGGCGGCGATatgaat                                                                                    <  1:39354/100‑1 (MQ=255)

          ataAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGAAGTTAATGGTTTCACCAGTGGCGATGTTCAGTGCCTCAAGATGCGGGGCGGCGATatgaatg                                                                                   <  1:20629/100‑1 (MQ=255)

            aaaTCAAAATAGCGTGATCGTCTTCGCGGCTGGAGAAGTTAATGGTTTCACCAGTGGCGATGTTCAGTGCCTCAAGATGCGGGGCGGCGATATGAATGat                                                                                 >  1:2080787/1‑100 (MQ=255)

            aaaTCAAAATAGCGTGATCGTCTTCGCGGCTGGAGAAGTTAATGGTTTCACCAGTGGCGATGTTCAGTGCCTCAAGATGCGGAGCGGCGATATGAATGat                                                                                 <  2:1963270/100‑1 (MQ=255)

             aaTCAAAATAGCGTGATCGTCTTCGCGGCTGGAGAAGTTAATGGTTTCACCAGTGGCGCTGTTCAGTGCCTCAAGATGCGGGGCGGCGATATGAATGata                                                                                >  2:2709939/1‑100 (MQ=255)

             aaTCAAAATAGCGTGATCGTCTTCGCGGCTGGAGAAGTTAATGGTTTCACCAGTGGCGATGTTCAGTGCCTCAAGATGCGGGGCGGCGATATGAATGata                                                                                >  1:1139904/1‑100 (MQ=255)

             aaTCAAAATAGCGTGATCGTCTTCGCGGCTGGAGAAGTTAATGGTTTCACCAGTGGCGATGTTCAGTGCCTCAAGATGCGGGGCGGCGATATGAATGata                                                                                >  2:2885549/1‑100 (MQ=255)

               ncAAAATAGCGTGATCGTCTTCGCGGCTGGAGAAGTTAATGGTTTCACCAGTGGCGATGTTCAGTGCCTCAAGATGCGGGGCGGCGATATGAATGATAtt                                                                              >  2:1198182/2‑100 (MQ=255)

                cAAAATAGCGTGATCGTCTTCGCGGCTGGAGAAGTTAATGGTTTCACCAGTGGCGATGTTCAGTGCCTCAAGATGCGGGGCGGCGATATGAATGATATTc                                                                             <  1:1653772/100‑1 (MQ=255)

                 aaaaTAGCGTGATCGTCTTCGCGGCTGGAGAAGTTAATGGTTTCACCAGTGGCGATGTTCAGTGCCTCAAGATGCGGGGCGGCGATATGAATGATATTCa                                                                            <  2:596723/100‑1 (MQ=255)

                 aaaaTAGCGTGATCGTCTTCGCGGCTGGAGAAGTTAATGGTTTCACCAGTGGCGATGTTCAGTGCCTCAAGATGCGGGGCGGCGATATGAATGATATTCa                                                                            >  2:2055941/1‑100 (MQ=255)

                    aTAGCGTGATCGTCTTCGCGGCTGGAGAAGTTAATGGTTTCACCAGTGGCGATGTTCAGTGCCTCAAGATGCGGGGCGGCGATATGAATGATATTCAGCg                                                                         <  1:1248672/100‑1 (MQ=255)

                      aGCGTGATCGTCTTCGCGGCTGGAGAAGTTAATGGTTTCACCAGTGGCGATGTTCAGTGCCTCAAGATGCGGGGCGGCGATATGAATGATATTCAGCGaa                                                                       >  2:2319489/1‑100 (MQ=255)

                         gTGATCGTCTTCGCGGCTGGAGAAGTTAATGGTTTCACCAGTGGCGATGTTCAGTGCCTCAAGATGCGGGGCGGCGATATGAATGATATTCAGCGAAGAc                                                                    >  2:422868/1‑100 (MQ=255)

                             tCGTCTTCGCGGCTGGAGAAGTTAATGGTTTCACCAGTGGCGATGTTCAGTGCCTCAAGATGCGGGGCGGCGATATGAATGATATTCAGCGAAGACAgcg                                                                >  2:1700746/1‑100 (MQ=255)

                             tCGTCTTCGCGGCTGGAGAAGTTAATGGTTTCACCAGTGGCGATGTTCAGTGCCTCAAGATGCGGGGCGGCGATATGAATGATATTCAGCGAAGACAgcg                                                                <  1:2379413/100‑1 (MQ=255)

                             tCGTCTTCGCGGCTGGAGAAGTTAATGGTTACAGCAGTGGCGATGTTCAGTGCCTCAAGATGCGGGGCGGCGATATGAATGATATTCAGCGAAGACAgcg                                                                >  2:1898309/1‑100 (MQ=255)

                               gTCTTCGCGGCTGGAGAAGTTAATGGTTTCACCAGTGGCGATGTTCAGTGCCTCAAGATGCGGGGCGGCGATATGAATGATATTCAGCGAAGACAGCGcc                                                              >  1:1654182/1‑100 (MQ=255)

                                    cgcgGCTGGAGGAGTTAATGGTTTCACCAGTGGCGATGTTCAGTGCCTCAAGATGCGGGGCGGCGATATGAATGATATTCAGCGAAGACAGCGCCTTCt                                                          >  2:1643605/1‑99 (MQ=255)

                                     gcgGCTGGAGAAGTTAATGGTTTCACCAGTGGCGATGTTCAGTGCCTCAAGATGCGGGGCGGCGATATGAATGATATTCAGCGAAGACAGCGCCTTCTGc                                                        <  1:2133685/100‑1 (MQ=255)

                                     gcgGCTGGAGAAGTTAATGGTTTCACCAGTGGCGATGTTCAGTGCCTCAAGATGCGGGGCGGCGATATGAATGATATTCAGCGAAGACAGCGCCTTCTGc                                                        <  1:2133411/100‑1 (MQ=255)

                                      cgGCTGGAGAAGTTAATGGTTTCACCAGTGGCGATGTTCAGTGCCTCAAGATGCGGGGCGGCGATATGAATGATATTCAGCGAAGACAGCGCCTTCTGcc                                                       <  2:965062/100‑1 (MQ=255)

                                       ggCTGGAGAAGTTAATGGTTTCACCAGTGGCGATGTTCAGTGCCTCAAGATGCGGGGCGGCGATATGAATGATATTCAGCGAAGACAGCGCCTTCTGccc                                                      <  1:1467306/100‑1 (MQ=255)

                                       ggCTGGAGAAGTTAATGGTTTCACCAGTGGCGATGTTCAGTGCCTCAAGATGCGGGGCGGCGATATGAATGATATTCAGCGAAGACAGCGCCTTCTGccc                                                      <  2:2724601/100‑1 (MQ=255)

                                         cTGGAGAAGTTAATGGTTTCACCAGTGGCGATGTTCAGTGCCTCAAGATGCGGGGCGGCGAGATGAATGATATTCAGCGAAGACAGCGCCTTCTGcc                                                       >  2:2412341/1‑97 (MQ=255)

                                          tGGAGAAGTTAATGGTTTCACCAGTGGCGATGTTCAGTGCCTCAAGATGCGGGGCGGCGATATGAATGATATTCAGCGAAGACAGCGCCTTCTGCCCGAc                                                   >  2:1778230/1‑100 (MQ=255)

                                            gagaAGTTAATGGTTTCACCAGTGGCGATGTTCAGTGCCTCAAGATGCGGGGCGGCGATATGAATGATATTCAGCGAAGACAGCGCCTTCTGCCCGACgg                                                 <  1:1179330/100‑1 (MQ=255)

                                            gagaAGTTAATGGTTTCACCAGTGGCGATGTTCAGTGCCTCAAGATGCGGGGCGGCGATATGAATGATATTCAGCGAAGACAGCGCCTTCTGCCCGACgg                                                 >  2:233231/1‑100 (MQ=255)

                                            gagaAGTTAATGGTTTCACCAGTGGCGATGTTCAGTGCCTCAAGATGCGGGGCGGCGATATGAATGATATTCAGCGAAGACAGCGCCTTCTGCCCGACgg                                                 <  1:1281003/100‑1 (MQ=255)

                                            gagaAGTTAATGGTTTCACCAGTGGCGATGTTCAGTGCCTCAAGATGCGGGGCGGCGATATGAATGATATTCAGCGAAGACAGCGCCTTCTGCCCGACgg                                                 >  2:233153/1‑100 (MQ=255)

                                            gagaAGTTAATGGTTTCACCAGTGGCGATGTTCAGTGCCTCAAGATGCGGGGCGGCGATATGAATGATATTCAGCGAAGACAGCGCCTTCTGCCCGACgg                                                 >  1:75085/1‑100 (MQ=255)

                                             agaAGTTAATGGTTTCACCAGTGGCGATGTTCAGTGCCTCAAGATGCGGGGCGGCGATATGAATGATATTCAGCGAAGACAGCGCCTTCTGCCCGACGGc                                                <  1:2390127/100‑1 (MQ=255)

                                             agaAGTTAATGGTTTCACCAGTGGCGATGTTCAGTGCCTCAAGATGCGGGGCGGCGATATGAATGATATTCAGCGAAGACAGCGCCTTCTGCCCGACGGc                                                <  1:1795539/100‑1 (MQ=255)

                                              gaAGTTAATGGTTTGACCAGTGGCGATGTTCAGTGCCTCAAGATGCGGGGCGGCGATATGAATGATATTCAGCGAAGACAGCGCCTTCTGCCCGACGGc                                                <  2:1726001/99‑1 (MQ=255)

                                              gaAGTTAATGGTTTCACCAGTGGCGATGTTCAGTGCCTCAAGATGCGGGGCGGCGATATGAATGATATTCAGCGAAGACAGCGCCTTCTGCCCGACGGCa                                               <  2:603301/100‑1 (MQ=255)

                                              gaAGTTAATGGTTTCACCAGTGGCGATGTTCAGTGCCTCAAGATGCGGGGCGGCGATATGAATGATATTCAGCGAAGACAGCGCCTTCTGCCCGACGGCa                                               <  1:661728/100‑1 (MQ=255)

                                                aGTTAATGGTTTCACCAGTGGCGATGTTCAGTGCCTCAAGATGCGGGGCGGCGATATGAATGATATTCAGCGAAGACAGCGCCTTCTGCCCGACGGCAAt                                             >  2:1557066/1‑100 (MQ=255)

                                                aGTTAATGGTTTCACCAGTGGCGATGTTCAGTGCCTCAAGATGCGGGGCGGCGATATGAATGATATTCAGCGAAGACAGCGCCTTCTGCCCGACGGCAAt                                             >  1:609547/1‑100 (MQ=255)

                                                 gTTAATGGTTTCACCAGTGGCGATGTTCAGTGCCTCAAGATGCGGGGCGGCGATATGAATGATATTCAGCGAAGACAGCGCCTTCTGCCCGACGGCAATa                                            >  1:1189283/1‑100 (MQ=255)

                                                 gTTAATGGTTTCACCAGTGGCGATGTTCAGTGCCTCAAGATGCGGGGCGGCGATATGAATGATATTCAGCGAAGACAGCGCCTTCTGCCCGACGGCAATa                                            >  1:998901/1‑100 (MQ=255)

                                                    aaTGGTTTCACCAGTGGCGATGTTCAGTGCCTCAAGATGCGGGGCGGCGATATGAATGATATTCAGCGAAGACAGCGCCTTCTGCCCGACGGCAATAAAt                                         <  2:766621/100‑1 (MQ=255)

                                                    aaTGGTTTCACCAGTGGCGATGTTCAGTGCCTCAAGATGCGGGGCGGCGATATGAATGATATTCAGCGAAGACAGCGCCTTCTGCCCGACGGCAATAAAt                                         >  2:533082/1‑100 (MQ=255)

                                                    aaTGGTTTCACCAGTGGCGATGTTCAGTGCCTCAAGATGCGGGGCGGCGATATGAATGATATTCAGCGAAGACAGCGCCTTCTGCCCGACGGCAATAAAt                                         <  2:1265058/100‑1 (MQ=255)

                                                     aTGGTTTCACCAGTGGCGATGTTCAGTGCCTCAAGATGCGGGGCGGCGATATGAATGATATTCAGCGAAGACAGCGCCTTCTGCCCGACGGCAATAAAtt                                        >  1:2312287/1‑100 (MQ=255)

                                                     aTGGTTTCACCAGTGGCGATGTTCAGTGCCTCAAGATGCGGGGCGGCGATATGAATGATATTCAGCGAAGACAGCGCCTTCTGCCCGACGGCAATAAAtt                                        <  1:2348782/100‑1 (MQ=255)

                                                      tGGTTTCACCAGTGGCGATGTTCAGTGCCTCAAGATGCGGGGCGGCGATATGAATGATATTCAGCGAAGACAGCGCCTTCTGCCCGACGGCAATAAAttt                                       >  2:2255395/1‑100 (MQ=255)

                                                       ggTTTCACCAGTGGCGATGTTCAGTGCCTCAAGATGCGGGGCGGCGATATGAATGATATTCAGCGAAGACAGCGCCTTCTGCCCGACGGCAATAAATTtg                                      >  1:1191267/1‑100 (MQ=255)

                                                       ggTTTCACCAGTGGCGATGTTCAGTGCCTCAAGATGCGGGGCGGCGATATGAATGATATTCAGCGAAGACAGCGCCTTCTGCCCGACGGCAATAAATTtg                                      >  1:351203/1‑100 (MQ=255)

                                                       ggTTTCACCAGTGGCGATGTTCAGTGCCTCAAGATGCGGGGCGGCGATATGAATGATATCCAGCGAAGACAGCGCCTTCTGCCCGACGGCAATAAATTtg                                      >  2:2624446/1‑100 (MQ=255)

                                                          ttCACCAGTGGCGATGTTCAGTGCCTCAAGATGCGGGGCGGCGATATGAATGATATTCAGCGAAGACAGCGCCTTCTGCCCGACGGCAATAAATTtggtg                                   <  1:1204116/100‑1 (MQ=255)

                                                            cACCAGTGGCGATGTTCAGTGCCTCAAGATGCGGGGCGGCGATATGAATGATATTCAGCGAAGACAGCGCCTTCTGCCCGACGGCAATAAATTTggtggt                                 >  1:1092158/1‑100 (MQ=255)

                                                            cACCAGTGGCGATGTTCAGTGCCTCAAGATGCGGGGCGGCGATATGAATGATATTCAGCGAAGACAGCGCCTTCTGCCCGACGGCAATAAATTTggtggt                                 >  1:1484720/1‑100 (MQ=255)

                                                            cACCAGTGGCGATGTTCAGTGCCTCAAGATGCGGGGCGGCGATATGAATGATATTCAGCGAAGACAGCGCCTTCTGCCCGACGGCAATAAATTTggtggt                                 >  1:1484825/1‑100 (MQ=255)

                                                             aCCAGTGGCGATGTTCAGTGCCTCAAGATGCGGGGCGGCGATATGAATGATATTCAGCGAAGACAGCGCCTTCTGCCCGACGGCAATAAATTTGGTGGTc                                >  1:2398486/1‑100 (MQ=255)

                                                             aCCAGTGGCGATGTTCAGTGCCTCAAGATGCGGGGCGGCGATATGAATGATATTCAGCGAAGACAGCGCCTTCTGCCCGACGGCAATAAATTTGGTGGTc                                <  2:1662517/100‑1 (MQ=255)

                                                                 gTGGCTATGTTCAGTGCCTAAAGATGCGGGGCGTCTATATAAATGATATTCAGCGAAGATAGCGCCTTCTGCCCGACGGCAATAAATTTGGTGGTCAGGc                            <  2:2564624/100‑1 (MQ=255)

                                                                 gTGGCGATGTTCAGTGCCTCAAGATGCGGGGCGGCGATATGAATGATATTCCGCGAAGACAGCGCCTTCTGCCCGACGGCAACAAATTTCGTGGTCAGGc                            >  1:556587/1‑100 (MQ=255)

                                                                 gTGGCGATGTTCAGTGCCTCAAGATGCGGGGCGGCGATATGAATGATATTCAGCGAAGACAGCGCCTTCTGCCCGACGGCAATAAATTTGGTGGTCAGGc                            <  1:2247115/100‑1 (MQ=255)

                                                                 gTGGCGATGTTCAGTGCCTCAAGATGCGGGGCGGCGATATGAATGATATTCAGCGAAGACAGCGCCTTCTGCCCGACGGCAATAAATTTGGTGGTCAGGc                            <  1:422869/100‑1 (MQ=255)

                                                                   ggCGATGTTCAGTGCCTCAAGATGCGGGGCGGCGATATGAATGATATTCAGCGAAGACAGCGCCTTCTGCCCGACGGCAATAAATTTGGTGGTCAGGCGa                          >  1:474479/1‑100 (MQ=255)

                                                                   ggCGATGTTCAGTGCCTCAAGATGCGGGGCGGCGATATGAATGATATTCAGCGAAGACAGCGCCTTCTGCCCGACGGCAATAAATTTGGTGGTCAGGCGa                          >  1:1313563/1‑100 (MQ=255)

                                                                       aTGTTCAGTGCCTCAAGATGCGGGGCGGCGATATGAATGATATTCAGCGAAGACAGCGCCTTCTGCCCGACGGCAATAAATTTGGTGGTCAGGCGATAAc                      <  2:875210/100‑1 (MQ=255)

                                                                        tGTTCAGTGCCTCAAGATGCGGGGCGGCGATATGAATGATATTCAGCGAAGACAGCGCCTTCTGCCCGACGGCAATAAATTTGGTGGTCAGGCGATAACt                     <  2:2192111/100‑1 (MQ=255)

                                                                        tGTTCAGTGCCTCAAGATGCGGGGCGGCGATATGAATGATATTCAGCGAAGACAGCGCCTTCTGCCCGACGGCAATAAATTTGGTGGTCAGGCGATAACt                     >  1:946945/1‑100 (MQ=255)

                                                                         gTTCAGTGCCTCAAGATGCGGGGCGGCGATATGAATGATATTCAGCGAAGACAGCGCCTTCTGCCCGACGGCAATAAATTTGGTGGTCAGGCGATAACTc                    >  1:2525246/1‑100 (MQ=255)

                                                                         gTTCAGTGCCTCAAGATGCGGGGCGGCGATATGAATGATATTCAGCGAAGACAGCGCCTTCTGCCCGACGGCAATAAATTTGGTGGTCAGGCGATAACTc                    >  1:1576821/1‑100 (MQ=255)

                                                                          ttCAGTGCCTCAGGATGCGGGGCGGCGATATGAATGATATTCAGCGAAGACAGCGCCTTCTGCCCGACGGCAATAAATTTGGTGGTCAGGCGATAACTcc                   >  1:1899512/1‑100 (MQ=255)

                                                                           tCAGTGCCTCAAGATGCGGGGCGGCGATATGAATGATATTCAGCGAAGACAGCGCCTTCTGCCCGACGGCAATAAATTTGGTGGTCAGGCGATAACTccc                  <  1:1198186/100‑1 (MQ=255)

                                                                              gTGCCTCAAGATGCGGGGCGGCGATATGAATGATATTCAGCGAAGACAGCGCCTTCTGCCCGACGGCAATAAATTTGGTGGTCAGGCGATAACTCCCTGc               >  2:2455380/1‑100 (MQ=255)

                                                                                gCCTCAAGATGCGGGGCGGCGATATGAATGATATTCAGCGAATACAGCGCCTTCTGCCCGACGGCAATAAATTTGGTGGTCAGGCGATAACTCCCTGCgg             <  2:2026723/100‑1 (MQ=255)

                                                                                gCCTCAAGATGCGGGGCGGCGATATGAATGATATTCAGCGAAGACAGCGCCTTCTGCCCGACGGCAATAAATTTGGTGGTCAGGCGATAACTCCCTGCgg             >  1:2419893/1‑100 (MQ=255)

                                                                                gCCTCAAGATGCGGGGCGGCGATATGAATGATATTCAGCGAAGACAGCGCCTTCTGCCCGACGGCAATAAATTTGGTGGTCAGGCGATAACTCCCTGCgg             >  1:994075/1‑100 (MQ=255)

                                                                                gCCTCAAGATGCGGGGCGGCGATATGAATGATATTCAGCGAAGACAGCGCCTTCTGCCCGACGGCAATAAATTTGGTGGTCAGGCGATAACTCCCTGCgg             >  2:2872886/1‑100 (MQ=255)

                                                                                gCCTCAAGATGCGGGGCGGCGATATGAATGATATTCAGCGAAGACAGCGCCTTCTGCCCGACGGCAATAAATTTGGTGGTCAGGCGATAACTCCCTGCgg             >  1:2538502/1‑100 (MQ=255)

                                                                                 ccTCAAGATGCGGGGCGGCGATATGAATGATATTCAGCGAAGACAGCGCCTTCTGCCCGACGGCAATAAATTTGGTGGTCAGGCGATAACTCCCTGCGGc            <  1:968293/100‑1 (MQ=255)

                                                                                 ccTCAAGATGCGGGGCGGCGATATGAATGATATTCAGCGAAGACAGCGCCTTCTGCCCGACGGCAATAAATTTGGTGGTCAGGCGATAACTCCCTGCGGc            >  1:488067/1‑100 (MQ=255)

                                                                                  cTCAAGATGCGGGGCGGCGATATGAATGATATTCAGCGAAGACAGCGCCTTCTGCCCGACGGCAATAAATTTGGTGGTCAGGCGATAACTCCCTGCGGCa           >  1:1105842/1‑100 (MQ=255)

                                                                                   tCAAGATGCGGTGCGGCGATATGAATGATATTCAGCGAAGACAGCGCCTTCTGCCCGACGGCAATAAATTTGGTGGTCAGGCGATAACTCCCTGCGGCAg          <  2:2449877/100‑1 (MQ=255)

                                                                                   tCAAGATGCGGGGCGGCGATATGAATGATATTCAGCGAAGACAGCGCCTTCTGCCCGACGGCAATAAATTTGGTGGTCAGGCGATAACTCCCTGCGGCAg          >  2:254031/1‑100 (MQ=255)

                                                                                    cAAGATGCGGGGCGGCGATATGAATGATATTCAGCGAAGACAGCGCCTTCTGCCCGACGGCAATAAATTTGGTGGTCAGGCGATAACTCTCTGCGGCAgg         >  2:1894004/1‑100 (MQ=255)

                                                                                    cAAGATGCGGGGCGGCGATATGAATGATATTCAGCGAAGACAGCGCCTTCTGCCCGACGGCAATAAATTTGGTGGTCAGGCGATAACTCCCTGCGGCAgg         >  1:1294756/1‑100 (MQ=255)

                                                                                    cAAGATGCGGGGCGGCGATATGAATGATATTCAGCGAAGACAGCGCCTTCTGCCCGACGGCAATAAATTTGGTGGTCAGGCGATAACTCCCTGCGGCAgg         >  1:123987/1‑100 (MQ=255)

                                                                                    cAAGATGCGGGGCGGCGATATGAATGATATTCAGCGAAGACAGCGCCTTCTGCCCGACGGCAATAAATTTGGTGGTCAGGCGATAACTCCCTGCGGCAgg         >  1:1848007/1‑100 (MQ=255)

                                                                                    cAAGATGCGGGGCGGCGATATGAATGATATTCAGCGAAGACAGCGCCTTCTGCCCGACGGCAATAAATTTGGTGGTCAGGCGATAACTCCCTGCGGCAgg         >  2:639617/1‑100 (MQ=255)

                                                                                         tGCGGGGCGGCGATATGAATGATATTCAGCGAAGACAGCGCCTTCTGCCCGACGGCAATAAATTTGGTGGTCAGGCGATAACTCCCTGCGGCAGGCGCgg    <  1:2455396/100‑1 (MQ=255)

                                                                                          gCGGGGCGGCGATATGAATGATATTCAGCGAAGACAGCGCCTTCTGCCCGACGGCAATAAATTTGGTGGTCAGGCGATAACTCCCTGCGGCAGGCGCggt   <  2:1706075/100‑1 (MQ=255)

                                                                                           cGGGGCGGCGATATGAATGATATTCAGCGAAGACAGCGCCTTCTGCCCGACGGCAATAAATTTGGTGGTCAGGCGATAACTCCCTGCGGCAGGCGCggtg  >  1:1745192/1‑100 (MQ=255)

GTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGAAGTTAATGGTTTCACCAGTGGCGATGTTCAGTGCCTCAAGATGCGGAGCGGCGATATGAATGATATTCAGCGAAGACAGCGCCTTCTGCCCGACGGCAATAAATTTGGTGGTCAGGCGATAACTCCCTGCGGCAGGCGCGGTG  >  NC_000913/3742124‑3742314

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A