Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A16 F258 I1 R1	132	116.1	5785507	94.2%	5449947	99.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,343,821	(T)_7→6	coding (453/1422 nt)	melB →	melibiose:sodium symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,343,815	0	T	.	96.4%	341.0 / 5.1	83	coding (447/1422 nt)	melB	melibiose:sodium symporter
Reads supporting (aligned to +/- strand): ref base T (3/0); new base . (34/46); total (37/46)
Fisher's exact test for biased strand distribution p-value = 8.46e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 104 total aligned reads displayed.

GGCATGACTTACACCATTATGGATATTCCCTTCTGGTCGCTGGTTCCAACCATCACGCTCGATAAACGTGAGCGCGAACAACTGGTTCCTTATCCGCGTTTTTTTGCCAGTCTGGCAGGCTTTGTTACGGCAGGTGTGACGCTACCATTTGTTAATTATGTCGGCGGTGGCGATCGGGGATTTGGCTTTCAGAT  >  NC_000913/4343717‑4343910

ggCATGACTTACACCATTATGGATATTCCCTTCTGGTCGCTGGTTCCAACCATCACGCTCGATAAACGTGAGCGCGAACAACTGGTTCCTTATCCGCGtt                                                                                                >  2:1386886/1‑100 (MQ=255)

ggCATGACTTACACCATTATGGATATTCCCTTCTGGTCGCTGGTTCCAACCATCACGCTCGATAAACGTGAGCGCGAACAACTGGTTCCTTATCCGCGtt                                                                                                >  1:81969/1‑100 (MQ=255)

ggCATGACTTACACCATTATGGATATTCCCTTCTGGTCGCTGGTTCCAACCATCACGCTCGATAAACGTGAGCGCGAACAACTGGTTCCTTATCCGCGtt                                                                                                >  1:455034/1‑100 (MQ=255)

ggCATGACTTACACCATTATGGATATTCCCTTCTGGTCGCTGGTTCCAACCATCACGCTCGATAAACGTGAGCGCGAACAACTGGTTCCTTATCCGCGtt                                                                                                >  1:1906652/1‑100 (MQ=255)

ggCATGACTTACACCATTATGGATATTCCCTTCTGGTCGCTGGTTCCAACCATCACGCTCGATAAACGTGAGCGCGAACAACTGGTTCCTTATCCGCGtt                                                                                                <  1:1891123/100‑1 (MQ=255)

ggCATGACTTACACCATTATGGATATTCCCTTCTGGTCGCTGGTTCCAACCATCACGCTCGATAAACGTGAGCGCGAACAACTGGTTCCTTATCCGCGtt                                                                                                <  2:2001737/100‑1 (MQ=255)

 gCATGACTTACACCATTATGGATATTCCCTTCTGGTCGCTGGTTCCAACCATCACGCTCGATAAACGTGAGCGCGAACAACTGGTTCCTTATCCGCGtt                                                                                                >  1:2359169/1‑99 (MQ=255)

  cATTACTTACACCATTATGGATATTCCCTTCTGGTCGCTGGTTCCAACCATCACGCTCGATAAACGTGAGCGCGAACAACTGGTTCCTTATCCGCGtttt                                                                                              <  2:496956/100‑1 (MQ=255)

    tGACTTACACCATTATGGATATTCCCTTCTGGTCGCTGGTTCCAACCATCACGCTCGATAAACGTGAGCGCGAACAACTGGTTCCTTATCCGCGtttttt                                                                                            <  2:2738009/100‑1 (MQ=255)

    tGACTTACACCATTATGGATATTCCCTTCTGGTCGCTGGTTCCAACCATCACGCTCGATAAACGTGAGCGCGAACAACTGGTTCCTTATCCGCGtttttt                                                                                            <  2:627585/100‑1 (MQ=255)

    tGACTTACACCATTATGGATATTCCCTTCTGGTCGCTGGTTCCAACCATCACGCTCGATAAACGTGAGCGCGAACAACTGGTTCCTTATCCGCGtttttt                                                                                            >  2:2748468/1‑100 (MQ=255)

     gACTTACACCATTATGGATATTCCCTTCTGGTCGCTGGTTCCAACCATCACGCTCGATAAACGTGAGCGCGAACAACTGGTTCCTTATCCGCGttttttg                                                                                           <  1:2294138/100‑2 (MQ=255)

     gACTTACACCATTATGGATATTCCCTTCTGGTAGCTGGTACCTACCATCACGCTCGATAAACGTGAGCGCGAACAACTGGTTCCTTATCCGCGttttttg                                                                                           <  2:2026227/100‑2 (MQ=255)

      aCTTACACCATTATGGATATTCCCTTCTGGTCGCTGGTTCCAACCATCACGCTCGATAAACGTGAGCGCGAACAACTGGTTCCTTATCCGCGttttttgc                                                                                          <  1:2236078/100‑3 (MQ=255)

       cTTACACCATTATGGATATTCCCTTCTGGTCGCTGGTTCCAACCATCACGCTCGATAAACGTGAGCGCGAACAACTGGTTCCTTATCCGCGttttttgcc                                                                                         <  1:1630658/100‑4 (MQ=255)

       cTTACACCATTATGGATATTCCCTTCTGGTCGCTGGTTCCAACCATCACGCTCGATAAACGTGAGCGCGAACAACTGGTTCCTTATCCGCGttttttgcc                                                                                         <  2:573911/100‑4 (MQ=255)

       cTTACACCATTATGGATATTCCCTTCTGGTCGCTGGTTCCAACCATCACGCTCGATAAACGTGAGCGCGAACAACTGGTTCCTTATCCGCGttttttgcc                                                                                         <  1:67160/100‑4 (MQ=255)

       cTTACACCATTATGGATATTCCCTTCTGGTCGCTGGTTCCAACCATCACGCTCGATAAACGTGAGCGCGAACAACTGGTTCCTTATCCGCGttttttgcc                                                                                         >  1:1380618/1‑97 (MQ=255)

       cTTACACCATTATGGATATTCCCTTCTGGTCGCTGGTTCCAACCATCACGCTCGATAAACGTGAGCGCGAACAACTGGTTCCTTATCCGCGttttttgcc                                                                                         >  2:323223/1‑97 (MQ=255)

         tACACCATTATGGATATTCCCTTCTGGTCGCTGGTTCCAACCATCACGCTCGATAAACGTGAGCGCGAACAACTGGTTCCTTATCCGCG‑TTTTTTGCCAg                                                                                      <  2:604644/100‑1 (MQ=255)

         tACACCATTATGGATATTCCCTTCTGGTCGCTGGTTCCAACCATCACGCTCGATAAACGTGAGCGCGAACAACTGGTTCCTTATCCGCG‑TTTTTTGCCAg                                                                                      >  1:2439546/1‑100 (MQ=255)

           cacCATTATGGATATTCCCTTCTGGTCGCTGGTTCCAACCATCACGCTCGATAAACGTGAGCGCGAACAACTGGTTCCTTATCCGCG‑TTTTTTGCCAGTc                                                                                    >  1:2383866/1‑100 (MQ=255)

           cacCATTATGGATATTCCCTTCTGGTCGCTGGTTCCAACCATCACGCTCGATAAACGTGAGCGCGAACAACTGGTTCCTTATCCGCG‑TTTTTTGCCAGTc                                                                                    >  1:1682045/1‑100 (MQ=255)

               aTTATGGATATTCCCTTCTGGTCGCTGGTTCCAACCATCACGCTCGATAAACGTGAGCGCGAACAACTGGTTCCTTATCCGCGtttttttttttttt                                                                                    >  2:1369936/1‑90 (MQ=255)

               aTTATGGATATTCCCTTCTGGTCGCTGGTTCCAACCATCACGCTCGATAAACGTGAGCGCGAACAACTGGTTCCTTATCCGCG‑TTTTTTGCCAGTCTGGc                                                                                <  2:2781928/100‑1 (MQ=255)

                 tATGGATATTCCCTTCTGGTCGCTGGTTCCAACCATCACGCTCGATAAACGTGAGCGCGAACAACTGGTTCCTTATCCGCG‑TTTTTTGCCAGTCTGGCAg                                                                              <  1:841913/100‑1 (MQ=255)

                 tATGGATATTCCCTTCTGGTCGCTGGTTCCAACCATCACGCTCGATAAACGTGAGCGCGAACAACTGGTTCCTTATCCGCG‑TTTTTTGCCAGTCTGGCAg                                                                              >  1:2546749/1‑100 (MQ=255)

                   tGGATATTCCCTTCTGGTCGCTGGTTCCAACCATCACGCTCGATAAACGTGAGCGCGAACAACTGGTTCCTTATCCGCG‑TTTTTTGCCAGTCTGGCAGGc                                                                            >  2:116111/1‑100 (MQ=255)

                   tGGATATTCCCTTCTGGTCGCTGGTTCCAACCATCACGCTCGATAAACGTGAGCGCGAACAACTGGTTCCTTATCCGCG‑TTTTTTGCCAGTCTGGCAGGc                                                                            >  2:1596658/1‑100 (MQ=255)

                     gATATTCCCTTCTGGTCGCTGGTTCCAACCATCACGCTCGATAAACGTGAGCGCGAACAACTGGTTCCTTATCCGCG‑TTTTTTGCCAGTCTGGCAGGCtt                                                                          >  2:364227/1‑100 (MQ=255)

                      atatTCCCTTCTGGTCGCTGGTTCCAACCATCACGCTCGATAAACGTGAGCGCGAACAACTGGTTCCTTATCCGCG‑TTTTTTGCCAGTCTGGCAGGCttt                                                                         <  2:1625412/100‑1 (MQ=255)

                       tatTCCCTTCTGGTCGCTGGTTCCAACCATCACGCTCGATAAACGTGAGCGCGAACAACTGGTTCCTTATCCGCG‑TTTTTTGCCAGTCTGGCAGGCTTTg                                                                        <  2:2554361/100‑1 (MQ=255)

                        atTCCCTTCTGGTCGCTGGTTCCAACCATCACGCTCGATAAACGTGAGCGCGAACAACTGGTTCCTTATCCGCG‑TTTTTTGCCAGTCTGGCGGGCTTTGt                                                                       >  1:2596491/1‑100 (MQ=255)

                         ttCCCTTCTGGTCGCTGGTTCCAACCATCACGCTCGATAAACGTGAGCGCGAACAACTGGTTCCTTATCCGCG‑TTTTTTGCCAGTCTGGCAGGCTTTGtt                                                                      <  1:2099491/100‑1 (MQ=255)

                           cccTTCTGGTCGCTGGTTCCAACCATCACGCTCGATAAACGTGAGCGCGAACAACTGGTTCCTTATCCGCG‑TTTTTTGCCAGTCTGGCAGGCTTTGTTAc                                                                    >  2:1617567/1‑100 (MQ=255)

                           cccTTCTGGTCGCTGGTTCCAACCATCACGCTCGATAAACGTGAGCGCGAACAACTGGTTCCTTATCCGCG‑TTTTTTGCCAGTCTGGCAGGCTTTGTTAc                                                                    >  1:1117579/1‑100 (MQ=255)

                              ttCTGGTCGCTGGTTCCAACCATCACGCTCGATAAACGTGAGCGCGAACAACTGGTTCCTTATCCGCG‑TTTTTTGCCAGTCTGGCAGGCTTTGTTACGGc                                                                 <  1:1507898/100‑1 (MQ=255)

                                cTGGTCGCTGGTTCCAACCATCACGCTCGATAAACGTGAGCGCGAACAACTGGTTCCTTATCCGCG‑TTTTTTGCCAGTCTGGCAGGCTTTGTTACGGCAg                                                               <  1:2010530/100‑1 (MQ=255)

                                 tGGTCGCTGGTTCCAACCATCACGCTCGATAAACGTGAGCGCGAACAACTGGTTCCTTATCCGCG‑TTTTTTGCCAGTCTGGCAGGCTTTGTTACGGCAgg                                                              <  2:1641690/100‑1 (MQ=255)

                                  ggTCGCTGGTTCCAACCATCACGCTCGATAAACGTGAGCGCGAACAACTGGTTCCTTATCCGCG‑TTTTTTGCCAGTCTGGCAGGCTTTGTTACGGCAGgt                                                             >  1:1727173/1‑100 (MQ=255)

                                   gTCGCTGGTTCCAACCATCACGCTCGATAAACGTGAGCGCGAACAACTGGTTCCTTATCCGCG‑TTTTTTGCCAGTCTGGCAGGCTTTGTTACGGCAGgtg                                                            <  1:2510123/100‑1 (MQ=255)

                                   gTCGCTGGTTCCAACCATCACGCTCGATAAACGTGAGCGCGAACAACTGGTTCCTTATCCGCG‑TTTTTTGCCAGTCTGGCAGGCTTTGTTACGGCAGgtg                                                            <  1:992444/100‑1 (MQ=255)

                                   gTCGCTGGTTCCAACCATCACGCTCGATAAACGTGAGCGCGAACAACTGGTTCCTTATCCGCG‑TTTTTTGCCAGTCTGGCAGGCTTTGTTACGGCAGgtg                                                            <  2:1380613/100‑1 (MQ=255)

                                     cGCTGGTTCCAACCATCACGCTCGATAAACGTGAGCGCGAACAACTGGTTCCTTATCCGCG‑TTTTTTGCCAGTCTGGCAGGCTTTGTTACGGCAGGtgtg                                                          >  1:2475123/1‑100 (MQ=255)

                                     cGCTGGTTCCAACCATCACGCTCGATAAACGTGAGCGCGAACAACTGGTTCCTTATCCGCG‑TTTTTTGCCAGTCTGGCAGGCTTTGTTACGGCAGGtgtg                                                          >  1:163200/1‑100 (MQ=255)

                                     cGCTGGTTCCAACCATCACGCTCGAGAAACGTGAGCGCGAACAACTGGTTCCTTATCCGCG‑TTTTTTGCCAGTCTGGCAGGCTTTGTTACGGCAGGtgtg                                                          <  1:989903/100‑1 (MQ=255)

                                      gCTGGTTCCAACCATCACGCTCGATAAACGTGAGCGCGAACAACTGGTTCCTTATCCGCG‑TTTTTTGCCAGTCTGGCAGGCTTTGTTACGGCAGGTGTGa                                                         <  1:139427/100‑1 (MQ=255)

                                      gCTGGTTCCAACCATCACGCTCGATAAACGTGAGCGCGAACAACTGGTTCCTTATCCGCG‑TTTTTTGCCAGTCTGGCAGGCTTTGTTACGGCAGGTGTGa                                                         <  1:137961/100‑1 (MQ=255)

                                        tGGTTCCAACCATCACGCTCGATAAACGTGAGCGCGAACAACTGGTTCCTTATCCGCG‑TTTTTTGCCAGTCTGGCAGGCTTTGTTACGGCAGGTGTGACg                                                       <  2:103038/100‑1 (MQ=255)

                                          gTTCCAACCATCACGCTCGATAAACGTGAGCGCGAACAACTGGTTCCTTATCCGCG‑TTTTTTGCCAGTCTGGCAGGCTTTGTTACGGCAGGTGTGACGCt                                                     >  1:1084695/1‑100 (MQ=255)

                                           ttCCAACCATCACGCTCGATAAACGTGAGCGCGAACAACTGGTTCCTTATCCGCG‑TTTTTTGCCAGTCTGGCAGGCTTTGTTACGGCAGGTGTGACGCTa                                                    >  1:1625434/1‑100 (MQ=255)

                                            tCCAACCATCACGCTCGATAAACGTTAGCGCGAACAACTGGTTCCTTATCCGCG‑TTTTTTGCCAGTCTGGCAGGCTTTGTTACGGCAGGTGTGACGCTAc                                                   <  2:119369/100‑1 (MQ=255)

                                            tCCAACCATCACGCTCGATAAACGTGAGCGCGAACAACTGGTTCCTTATCCGCG‑TTTTTTGCCAGTCTGGCAGGCTTTGTTACGGCAGGTGTGACGCTAc                                                   <  2:592010/100‑1 (MQ=255)

                                            tCCAACCATCACGCTCGATAAACGTGAGCGCGAACAACTGGTTCCTTATCCGCG‑TTTTTTGCCAGTCTGGCAGGCTTTGTTACGGCAGGTGTGACGCTAc                                                   >  1:719002/1‑100 (MQ=255)

                                                 ccATCACGCTCGATAAACGTGAGCGCGAACAACTGGTTCCTTATCCGCGTTTTTTT‑TTTTTCTTTCAGGCTTTGTTACGGCAGGTGTGACGCTACCAttt                                              >  2:2145622/1‑100 (MQ=255)

                                                  cATCACGCTCGATAAGCGTGAGGGCGAACAACGTGTTCCTTAGCCGCG‑TTTTTTGCCAGTCTGGCAGGCGTTGTTACGGCAGGTGTGACGCTACCATTTg                                             <  1:2290452/100‑1 (MQ=255)

                                                  cATCACGCTCGATAAACGTGAGCGCGAACAACTGGTTCCTTATCCGCG‑TTTTTTGCCAGTCTGGCAGGCTTTGTTACGGCAGGTGTGACGCTACCATTTg                                             <  2:2439530/100‑1 (MQ=255)

                                                   aTCACGCTCGATAAACGTGAGCGCGAACAACTGGTTCCTTATCCGCG‑TTTTTTGCCAGTCTGGCAGGCTTTGTTACGGCAGGTGTGACGCTACCAt                                                >  1:2572488/1‑96 (MQ=255)

                                                   aTCACGCTCGATAAACGTGAGCGCGAACAACTGGTTCCTTATCCGCG‑TTTTTTGCCAGTCTGGCAGGCTTTGTTACGGCAGGTGTGACGCTACCATTTGt                                            <  2:2696877/100‑1 (MQ=255)

                                                   aTCACGCTCGATAAACGTGAGCGCGAACAACTGGTTCCTTATCCGCG‑TTTTTTGCCAGTCTGGCAGGCTTTGTTACGGCAGGTGTGACGCTACCATTTGt                                            >  1:1987656/1‑100 (MQ=255)

                                                     cACGCTCGATAAACGTGAGCGCGAACGACTGGTTCCTTATCCGCG‑TTTTTTGCCAGTCTGGCAGGCTTTGTTACGGCAGGTGTGACGCTACCATTTGTTa                                          >  1:2665967/1‑100 (MQ=255)

                                                     cACGCTCGATAAACGTGAGCGCGAACAACTGGTTCCTTATCCGCG‑TTTTTTGCCAGTCTGGCAGGCTTTGTTACGGCAGGTGTGACGCTACCATTTGTTa                                          <  2:1194350/100‑1 (MQ=255)

                                                     cACGCTCGATAAACGTGAGCGCGAACAACTGGTTCCTTATCCGCG‑TTTTTTGCCAGTCTGGCAGGCTTTGTTACGGCAGGTGTGACGCTACCATTTGTTa                                          <  2:1196759/100‑1 (MQ=255)

                                                      aCGCTCGATAAACGTGAGCGCGAACAACTGGTTCCTTATCCGCG‑TTTTTTGCCAGTCTGGCAGGCTTTGTTACGGCAGGTGTGACGCTACCATTTGTTaa                                         >  1:2308025/1‑100 (MQ=255)

                                                      aCGCTCGATAAACGTGAGCGCGAACAACTGGTTCCTTATCCGCG‑TTTTTTGCCAGTCTGGCAGGCTTTGTTACGGCAGGTGTGACGCTACCATTTGTTaa                                         <  2:2398239/100‑1 (MQ=255)

                                                        gCTCGATAAACGTGAGCGCGAACGACTGGTTCCTTATCCGCG‑TTTTTTGCCAGTCTGGCAGGCTTTGTTACGGCAGGTGTGACGCTACCATTTGTTAAtt                                       <  2:2665948/100‑1 (MQ=255)

                                                        gCTCGATAAACGTGAGCGCGAACAACTGGTTCCTTATCCGCG‑TTTTTTGCCAGTCTGGCAGGCTTTGTTACGGCAGGTGTGACGCTACCATTTGTTAAtt                                       >  1:958150/1‑100 (MQ=255)

                                                         cTCGATAAACGTGAGCGCGAACAACTGGTTCCTTATCCGCG‑TTTTTTGCCAGTCTGGCAGGCTTTGTTACGGCAGGTGTGACGCTACCATTTGTTAATTa                                      <  1:1461621/100‑1 (MQ=255)

                                                           cGATAAACGTGAGCGCGAACAACTGGTTCCTTATCCGCG‑TTTTTTGCCAGTCTGGCAGGCTTTGTTACGGCAGGTGTGACGCTACCATTTGTTAATTATg                                    <  1:2185790/100‑1 (MQ=255)

                                                            gATAAACGTGAGCGCGAACAACTGGTTCCTTATCCGCG‑TTTTTTGCCAGTCTGGCAGGCTTTGTTACGGCAGGTGTGACGCTACCATTTGTTAATTATGt                                   <  1:364228/100‑1 (MQ=255)

                                                              tAAACGTGAGCGCGAACAACTGGTTCCTTATCCGCG‑TTTTTTGCCAGTCTGGCAGGCTTTGTTACGGCAGGTGTGACGCTACCATTTGTTAATTATGTcg                                 >  1:2355605/1‑100 (MQ=255)

                                                              tAAACGTGAGCGCGAACAACTGGTTCCTTATCCGCG‑TTTTTTGCCAGTCTGGCAGGCTTTGTTACGGCAGGTGTGACGCTACCATTTGTTAATTATGTcg                                 >  1:2355647/1‑100 (MQ=255)

                                                                  cGTGAGCGCGAACAACTGGTTCCTTATCCGCG‑TTTTTTGCCAGTCTGGCAGGCTTTGTTACGGCAGGTGTGACGCTACCATTTGTTAATTATGTcggcgg                             >  1:2193042/1‑100 (MQ=255)

                                                                  cGTGAGCGCGAACAACTGGTTCCTTATCCGCG‑TTTTTTGCCAGTCTGGCAGGCTTTGTTACGGCAGGTGTGACGCTACCATTTGTTAATTATGTcggcgg                             <  2:1117575/100‑1 (MQ=255)

                                                                   gTGAGCGCGAACAACTGGTTCCTTATCCGCG‑TTTTTTGCCAGTCTGGCAGGCTTTGTTACGGCAGGTGTGACGCTACCATTTGTTAATTATGTCGGCGGt                            >  2:5332/1‑100 (MQ=255)

                                                                    tGAGCGCGAACAACTGGTTCCTTATCCGCG‑TTTTTTGCCAGTCTGGCAGGCTTTGTTACGGCAGGTGTGACGCTACCATTTGTTAATTATGTCGGCGGTg                           <  2:1932876/100‑1 (MQ=255)

                                                                      aGCGCGAACAACTGGTTCCTTATCCGCG‑TTTTTTGCCAGTCTGGCAGGCTTTGTTACGGCAGGTGTGACGCTACCATTTGTTAATTATGTCGGCGGTGGc                         <  1:592060/100‑1 (MQ=255)

                                                                       gcgcGAACAACTGGTTCCTTATCCGCG‑TTTTTTGCCAGTCTGGCAGGCTTTGTTACGGCAGGTGTGACGCTACCATTTGTTAATTATGTCGGCGGTGGCg                        >  1:1480682/1‑100 (MQ=255)

                                                                       gcgcGAACAACTGGTTCCTTATCCGCG‑TTTTTTGCCAGTCTGGCAGGCTTTGTTACGGCAGGTGTGACGCTACCATTTGTTAATTATGTCGGCGGTGGCg                        >  2:7159/1‑100 (MQ=255)

                                                                         gcgAACAACTGGTTCCTTATCCGCG‑TTTTTTGCCAGTCTGGCAGGCTTTGTTACGGCAGGTGTGACGCTACCATTTGTTAATTATGTCGGCGGTGGCGAt                      >  1:1732028/1‑100 (MQ=255)

                                                                          cgAACAACTGGTTCCTTATCCGCGTTTTTTTGCCAGTCTGACAGGCTTTGTTACGGCAGGTGTGAAGCTACCATTTGTTAATTATGTCCGCGGTGGCGa                       >  2:1397020/1‑99 (MQ=255)

                                                                          cgAACAACTGGTTCCATATCCGCG‑TTTTTTGCCAGTCTGGCAGGCTTTGTTACGGCAGGTGTGACGCTACCATTTGTTAATTATGTCGGCGGTGGCGATc                     <  1:814788/100‑1 (MQ=255)

                                                                            aacaacTGGTTCCTTATCCGCG‑TTTTTTGCCAGTCTGGCAGGCTTTGTTACGGCAGGTGTGACGCTACCATTTGTTAATTATGTCGGCGGTGGCGATCgg                   >  2:2022548/1‑100 (MQ=255)

                                                                            aacaacTGGTTCCTTATCCGCG‑TTTTTTGCCAGTCTGGCAGGCTTTGTTACGGCAGGTGTGACGCTACCATTTGTTAATTATGTCGGCGGTGGCGATCgg                   <  1:1596668/100‑1 (MQ=255)

                                                                             acaacTGGTTCCTTATCCGCG‑TTTTTTGCCAGTCTGGCAGGCTTTGTTACGGCAGGTGTGACGCTACCATTTGTTAATTATGTCGGCGGTGGCGATCggg                  <  1:789356/100‑1 (MQ=255)

                                                                              caacTGGTTCCTTATCCGCG‑TTTTTTGCCAGTCTGGCAGGCTTTGTTACGGCAGGTGTGACGCTACCATTTGTTAATTATGTCGGCGGTGGCGATCgggg                 <  1:641728/100‑1 (MQ=255)

                                                                               aacTTGTTCCTTATCCGCGTTTTTTT‑TTTTTTTTTCAGGCTTTGTTACGGCAAGTGTTATTCTTGTATTTTTTAATTATGTCGGCGGTGGCTATCg                    >  2:685968/1‑96 (MQ=255)

                                                                                    gTTCCTTATCCGCG‑TTTTTTGCCAGTCTGGCAGGCTTTGTTACGGCAGGTGTGACGCTACCATTTGTTAATTATGTCGGCGGTGGCGATCGGGGATTTgg           >  1:2719102/1‑100 (MQ=255)

                                                                                      tCCTTATGCGCG‑TTTTTTGCCAGTCTGGCAGGCTTTGGTACGGCAGGTGTGACGCTACCATTTGTTAATTATGTCGGCGGTGGCGATCGGGGATTTGGc          <  2:2572470/99‑1 (MQ=255)

                                                                                      tCCTTATCCGCG‑TTTTTTGCCAGTCTGGCAGGCTTTGTTACGGCAGGTGTGACGCTACCATTTGTTAATTATGTCGGCGGTGGCGATCGGGGATTTGGCt         >  2:2161347/1‑100 (MQ=255)

                                                                                      tCCTTATCCGCG‑TTTTTTGCCAGTCTGGCAGGCTTTGTTACGGCAGGTGTGACGCTACCATTTGTTAATTATGTCGGCGGTGGCGATCGGGGATTTGGCt         >  1:1418091/1‑100 (MQ=255)

                                                                                       ccTTATCCGCG‑TTTTTGGCCAGTCTGGCAGGCTTTGTTACGGCAGGTGTGACGCTACCATTTGTTAATTATGTCGGCGGTGGCGATCGGGGATTTGGCtt        <  2:258396/100‑1 (MQ=255)

                                                                                         ttATCCGCG‑TTTTTTGCCAGTCTGGCAGGCTTTGTTACGGCAGGTGTGACGCTTCCATTTGTTAATTATGTCGGCGGTGGCGATCGGGGATTTGGCTTTc      <  1:106274/100‑1 (MQ=255)

                                                                                         ttATCCGCG‑TTTTTTGCCAGTCTGGCAGGCTTTGTTACGGCAGGTGTGACGCTACCATTTGTTAATTATGTCGGCGGTGGCGATCGGGGATTTGGCTTTc      <  2:719000/100‑1 (MQ=255)

                                                                                          tATCCGCG‑TTTTTTGCCAGTCTGGCAGGCTTTGTTACGGCAGGTGTGACGCTACCATTTGTTAATTATGTCGGCGGTGGCGATCGGGGATTTGGCTTTCa     <  2:2475107/100‑1 (MQ=255)

                                                                                          tATCCGCG‑TTTTTTGCCAGTCTGGCAGGCTTTGTTACGGCAGGTGTGACGCTACCATTTGTTAATTATGTCGGCGGTGGCGATCGGGGATTTGGCTTTCa     <  1:17303/100‑1 (MQ=255)

                                                                                          tATCCGCG‑TTTTTTGCCAGTCTGGCAGGCTTTGTTACGGCAGGTGTGACGCTACCATTTGTTAATTATGTCGGCGGTGGCGATCGGGGATTTGGCTTTCa     <  1:1369941/100‑1 (MQ=255)

                                                                                            tCCGCG‑TTTTTTGCCAGTCTGGCAGGCTTTGTTACGGCAGGTGTGACGCTACCATTTGTTAATTATGTCGGCGGTGGCGATCGGGGATTTGGCTTTCAg    <  2:2174665/99‑1 (MQ=255)

                                                                                             ccGCG‑TTTTTTGCCAGTCTGGCAGGCTTTGTTACGGCAGGTGTGACGCTACCATTTGTTAATTATGTCGGCGGTGGCGATCGGGGATTTGGCTTTCAGAt  >  1:307718/1‑100 (MQ=255)

                                                                                             ccGCG‑TTTTTTGCCAGTCTGGCAGGCTTTGTTACGGCAGGTGTGACGCTACCATTTGTTAATTATGTCGGCGGTGGCGATCGGGGATTTGGCTTTCAGAt  >  1:1239135/1‑100 (MQ=255)

GGCATGACTTACACCATTATGGATATTCCCTTCTGGTCGCTGGTTCCAACCATCACGCTCGATAAACGTGAGCGCGAACAACTGGTTCCTTATCCGCGTTTTTTTGCCAGTCTGGCAGGCTTTGTTACGGCAGGTGTGACGCTACCATTTGTTAATTATGTCGGCGGTGGCGATCGGGGATTTGGCTTTCAGAT  >  NC_000913/4343717‑4343910

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A