Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A16 F258 I1 R1	132	116.1	5785507	94.2%	5449947	99.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,408,903	(A)_6→5	coding (2250/2442 nt)	rnr →	exoribonuclease R, RNase R

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,408,898	0	A	.	98.1%	448.6 / 1.2	104	coding (2245/2442 nt)	rnr	exoribonuclease R, RNase R
Reads supporting (aligned to +/- strand): ref base A (1/1); new base . (43/59); total (44/60)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.33e-01

Only 100 of 117 total aligned reads displayed.

GACGAGCGCAAAATCGACTTTAGCCTGATCTCCAGCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGTAAAAAAGGCGGCAAGCGTCGTCAGGTCGGTAAAAAGGTAAACTTTGAGCCAGACAGCGCCTTCCGCGGTGAGAAAAAAACGAAGCCGAAAGCGGCG  >  NC_000913/4408799‑4408993

gACGAGCGCAAAATCGACTTTAGCCTGATCTCCAGCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGTa                                                                                                 <  1:905318/100‑1 (MQ=255)

gACGAGCGCAAAATCGACTTTAGCCTGATCTCCAGCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGTa                                                                                                 <  1:1248871/100‑1 (MQ=255)

  cGAGCGCAAAATCGACTTTAGCCTGATCTCCAGCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGTaaa                                                                                               >  1:2294018/1‑100 (MQ=255)

  cGAGCGCAAAATCGACTTTAGCCTGATCTCCAGCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGTaaa                                                                                               >  1:1790549/1‑100 (MQ=255)

   gAGCGCAAAATCGACTTTAGCCTGATCTCCAGCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGTaaaa                                                                                              <  1:2454843/100‑1 (MQ=255)

    aGCGCAAAATCGACTTTAGCCTGATCTCCAGCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGTaaaaa                                                                                             >  1:1879899/1‑100 (MQ=255)

     gcgcAAAATCGACTTTAGCCTGATCTCCAGCGAACGCGCACCGCGCAACGGCGGTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGTaaaaag                                                                                            <  2:588817/100‑2 (MQ=255)

       gcAAAATCGACTTTAGCCTGATCTCCAGCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGGTGCAGGTaaaaaggc                                                                                          >  1:2844638/1‑97 (MQ=255)

       gcAAAATCGACTTTAGCCTGATCTCCAGCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGTaaaaaggc                                                                                          <  1:2256264/100‑4 (MQ=255)

       gcAAAATCGACTTTAGCCTGATCTCCAGCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGTaaaaagg                                                                                           >  2:488434/1‑97 (MQ=255)

        cAAAATCGACTTTAGCCGGATCTCCAGCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGTaaaaaggcg                                                                                         >  2:1954400/1‑96 (MQ=255)

            aTCGACTTTAGCCTGATCTCCAGCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGT‑AAAAAGGCGGCaa                                                                                    >  1:1102843/1‑100 (MQ=255)

            aTCGACTTTAGCCTGATCTCCAGCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGT‑AAAAAGGCGGCaa                                                                                    >  2:1208849/1‑100 (MQ=255)

            aTCGACTTTAGCCTGATCTCCAGCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGT‑AAAAAGGCGGCa                                                                                     >  2:74264/1‑99 (MQ=255)

              cGACTTTAGCCTGATCTCCAGCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGT‑AAAAAGGCGGCAAGc                                                                                  >  1:2786991/1‑100 (MQ=255)

              cGACTTTAGCCTGATCTCCAGCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGT‑AAAAAGGCGGCAAGc                                                                                  <  2:1329007/100‑1 (MQ=255)

                 cTTTAGCCTGATCTCCAGCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGT‑AAAAAGGCGGCAAGcgtc                                                                               >  2:1182912/1‑100 (MQ=255)

                 cTTTAGCCTGATCTCCAGCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGT‑AAAAAGGCGGCAAGcgtc                                                                               <  1:2221570/100‑1 (MQ=255)

                   ttAGCCTGATCTCCAGCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGT‑AAAAAGGCGGCAAGcgtcgt                                                                             >  1:98968/1‑100 (MQ=255)

                    tAGCCTGATCTCCAGCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGT‑AAAAAGGCGGCAAGCgtcgtc                                                                            <  2:882534/100‑1 (MQ=255)

                    tAGCCTGATCTCCAGCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGT‑AAAAAGGCGGCAAGCgtcgtc                                                                            >  2:944266/1‑100 (MQ=255)

                     aGCCTGATCTCCAGCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGT‑AAAAAGGCGGCAAGCGTCGTCa                                                                           <  1:1932203/100‑1 (MQ=255)

                      gCCTGATCTCCAGCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGT‑AAAAAGGCGGCAAGCGTCGTCAg                                                                          <  2:2388666/100‑1 (MQ=255)

                        cTGATCTCCAGCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGAT‑AAAAAGGCGGCAAGCGTCGTCAgg                                                                         >  2:481374/1‑99 (MQ=255)

                         tGATCTCCAGCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGT‑AAAAAGGCGGCAAGCGTCGTCAGGTc                                                                       <  1:211024/100‑1 (MQ=255)

                           aTCTCCAGCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGT‑AAAAAGGCGGCAAGCGTCGTCAGGTCgg                                                                     <  1:2797205/100‑1 (MQ=255)

                           aTCTCCAGCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGT‑AAAAAGGCGGCAAGCGTCGTCAGGTCgg                                                                     <  1:1911988/100‑1 (MQ=255)

                           aTCTCCAGCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGT‑AAAAAGGCGGCAAGCGTCGTCAGGTCgg                                                                     <  2:2021898/100‑1 (MQ=255)

                           aTCTCCAGCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGT‑AAAAAGGCGGCAAGCGTCGTCAGGTCgg                                                                     <  1:1039715/100‑1 (MQ=255)

                            tctcCAGCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGAAAAAAAAAAAAAAAAGGCGATGCAGGT‑AAAAAGGCGGCAAGCGTCGTCAGGTCGGt                                                                    <  2:1811984/100‑1 (MQ=255)

                             ctcCAGCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGT‑AAAAAGGCGGCAAGCGTCGTCAGGTCgg                                                                     <  2:905964/98‑1 (MQ=255)

                             ctcCAGCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGT‑AAAAAGGCGGCAAGCGTCGTCAGGTCGGTa                                                                   >  2:1502865/1‑100 (MQ=255)

                               ccAGCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGT‑AAAAAGGCGGCAAGCGTCGTCAGGTCGGaaaa                                                                 >  1:2756940/1‑100 (MQ=255)

                               ccAGCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGT‑AAAAAGGCGGCAAGCGTCGTCAGGTCGGTaaa                                                                 >  1:1943248/1‑100 (MQ=255)

                                cAGCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGT‑AAAAAGGCGGCAAGCGTCGTCAGGTCGGTaaaa                                                                >  1:2275635/1‑100 (MQ=255)

                                cAGCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGT‑AAAAAGGCGGCAAGCGTCGTCAGGTCGGTaaaa                                                                >  2:688299/1‑100 (MQ=255)

                                 aGCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGT‑AAAAAGGCGGCAAGCGTCGTCAGGTCGGTaaaaa                                                               >  2:1017160/1‑100 (MQ=255)

                                  gCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGT‑AAAAAGGCGGCAAGCGTCGTCAGGTCGGTAAAAAg                                                              <  1:150454/100‑1 (MQ=255)

                                   cGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGT‑AAAAAGGCGGCAAGCGTCGTCAGGTCGGTAAAAAgn                                                             <  2:920182/100‑2 (MQ=255)

                                   cGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGT‑AAAAAGGCGGCAAGCGTCGTCAGGTCGGTAAAAAgg                                                             <  2:38045/100‑1 (MQ=255)

                                     aaCGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGT‑AAAAAGGCGGCAAGCGTCGTCAGGTCGGTAAAAAGGTa                                                           <  1:2110771/100‑1 (MQ=255)

                                      aCGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGT‑AAAAAGGCGGCAAGCGTCGTCAGGTCGGTAAAAAGGTaa                                                          >  2:1497024/1‑100 (MQ=255)

                                       cgcgCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGT‑AAAAAGGCGGCAAGCGTCGTCAGGTCGGTAAAAAGGTaaa                                                         >  1:2469256/1‑100 (MQ=255)

                                       cgcgCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGT‑AAAAAGGCGGCAAGCGTCGTCAGGTCGGTAAAAAGGTaaa                                                         >  1:1663681/1‑100 (MQ=255)

                                         cgaaCCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGT‑AAAAAGGCGGCAAGCGTCGTCAGGTCGGTAAAAAGGTAAACt                                                       >  1:233623/4‑100 (MQ=255)

                                          gcACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGT‑AAAAAGGCGGCAAGCGTCGTCAGGTCGGTAAAAAGGTAAACtt                                                      >  1:15733/1‑100 (MQ=255)

                                           cACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGT‑AAAAAGGCGGCAAGCGTCGTCAGGTCGGTAAAAAGGTAAACttt                                                     >  1:2759381/1‑100 (MQ=255)

                                            aCCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGT‑AAAAAGGCGGCAAGCGTCGTCAGGTCGGTAAAAAGGTAAACTTTg                                                    <  1:279815/100‑1 (MQ=255)

                                             ccGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGT‑AAAAAGGCGGCAAGCGTCGTCAGGTCGGTAAAAAGGTAAACTTTGa                                                   <  1:536763/100‑1 (MQ=255)

                                             ccGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGT‑AAAAAGGCGGCAAGCGTCGTCAGGTCGGTAAAAAGGTAAACTTTGa                                                   <  1:1502873/100‑1 (MQ=255)

                                              cgcgCAACGTCGGTAAAACGGCTCGCGAGAATGCGAATAAAGGCGATGCAGGT‑AAAAAGGCGGCAAGCGTCGTCAGGTCGGTAAAAAGGTAAACTTTGAg                                                  <  2:1945568/100‑1 (MQ=255)

                                              cgcgCAACGTCGGTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGT‑AAAAAGGCGGCAAGCGTCGTCAGGTCGGTAAAAAGGTAAACTTTGAg                                                  <  2:1943189/100‑1 (MQ=255)

                                              cgcgCAACGTCGGTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGT‑AAAAAGGCGGCAAGCGTCGTCAGGTCGGTAAAAAGGTAAACTTTGAg                                                  <  2:806250/100‑1 (MQ=255)

                                              cgcgCAACGTCGGTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGT‑AAAAAGGCGGCAAGCGTCGTCAGGTCGGTAAAAAGGTAAACTTTGAg                                                  <  1:72347/100‑1 (MQ=255)

                                              cgcgCAACGTCGGTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGT‑AAAAAGGCGGCAAGCGTCGTCAGGTCGGTAAAAAGGTAAACTTTGAg                                                  >  2:526250/1‑100 (MQ=255)

                                               gcgcAACGTCGGTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGT‑AAAAAGGCGGCAAGCGTCGTCAGGTCGGTAAAAAGGTAAACTTTGAGc                                                 >  2:2176692/1‑100 (MQ=255)

                                               gcgcAACGTCGGTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGT‑AAAAAGGCGGCAAGCGTCGTCAGGTCGGTAAAAAGGTAAACTTTGAGc                                                 >  1:1232407/1‑100 (MQ=255)

                                                cgcAACGTCGGTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGT‑AAAAAGGCGGCAAGCGTCGTCAGGTCGGTAAAAAGGTAAACTTTGAGcc                                                <  2:2083116/100‑1 (MQ=255)

                                                cgcAACGTCGGTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGT‑AAAAAGGCGGCAAGCGTCGTCAGGTCGGTAAAAAGGTAAACTTTGAGcc                                                <  1:944267/100‑1 (MQ=255)

                                                cgcAACGTCGGTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGT‑AAAAAGGCGGCAAGCGTCGTCAGGTCGGTAAAAAGGTAAACTTTGAGcc                                                <  1:2230727/100‑1 (MQ=255)

                                                 gcAACGTCGGTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGT‑AAAAAGGCGGCAAGCGTCGTCAGGTCGGTAAAAAGGTAAACTTTGAGCCa                                               <  1:1208853/100‑1 (MQ=255)

                                                   aaCGTCGGTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGT‑AAAAAGGCGGCAAGCGTCGTCAGGTCGGTAAAAAGGTAAACTTTGAGCCAGa                                             <  1:2363614/100‑1 (MQ=255)

                                                     cGTCGGTAAAACGGCGCGCGAGAAAGAGAAAAAAGGCGATGCAGGT‑AAAAAGGCGGCAAGCGTCGTCAGGTCGGTAAAAAGGTAAACTTTGAGCCAGACa                                           >  2:2661380/1‑100 (MQ=255)

                                                         ggTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGT‑AAAAAGGCGGCAAGCGTCGTCAGGTCGGTAAAAAGGTAAACTTTGAGCCAGACAgcgc                                       <  2:293208/100‑1 (MQ=255)

                                                         ggTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGT‑AAAAAGGCGGCAAGCGTCGTCAGGTCGGTAAAAAGGTAAACTCTGAGCCAGACAgcgc                                       >  1:2842437/1‑100 (MQ=255)

                                                             aaaCGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGT‑AAAAAGGCGGCAAGCGTCGTCAGGTCGGTAAAAAGGTAAACTTTGAGCCAGACAGCGCCTTc                                   >  2:2394892/1‑100 (MQ=255)

                                                              accGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGT‑AAAAAGGCGGCAAGCGTCGTCAGGTCGGTAAAAAGGTAAACTTTGAGCCAGACAGCGCCTTcc                                  <  1:1064986/98‑1 (MQ=255)

                                                              aaCGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGT‑AAAAAGGCGGCAAGCGTCGTCAGGTCGGTAAAAAGGTAAACTTTGAGCCAGACAGCGCCTTcc                                  <  1:800988/100‑1 (MQ=255)

                                                                cGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGT‑AAAAAGGCGGCAAGCGTCGTCAGGTCGGTAAAAAGGTAAACTTTGAGCCAGACAGCGCCTTCcgc                                <  1:2804316/100‑1 (MQ=255)

                                                                cGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGT‑AAAAAGGCGGCAAGCGTCGTCAGGTCGGTAAAAAGGTAAACTTTGAGCCAGACAGCGCCTTCcgc                                <  2:70944/100‑1 (MQ=255)

                                                                  gcgcgcGAGAAAGCGAAAAAAGGCGATGCAGGT‑AAAAAGGCGGCAAGCGTCGTCAGGTCGGTAAAAAGGTAAACTTTGAGCCAGACAGCGCCTTCCGCgg                              <  2:643903/100‑1 (MQ=255)

                                                                  gcgcgcGAGAAAGCGAAAAAAGGCGATGCAGGT‑AAAAAGGCGGCAAGCGTCGTCAGGTCGGTAAAAAGGTAAACTTTGAGCCAGACAGCGCCTTCCGCgg                              <  1:763046/100‑1 (MQ=255)

                                                                  gcgcgcGAGAAAGCGAAAAAAGGCGATGCAGGT‑AAAAAGGCGGCAAGCGTCGTCAGGTCGGTAAAAAGGTAAACTTTGAGCCAGACAGCGCCTTCCGCgg                              <  1:1357176/100‑1 (MQ=255)

                                                                   cgcgcgAGAAAGCGAAAAAAGGCGATGCAGGT‑AAAAAGGCGGCAAGCGTCGTCAGGTCGGTAAAAAGGTAAACTTTGAGCCAGACAGCGCCTTCCGCGGt                             <  2:1403020/100‑1 (MQ=255)

                                                                   cgcgcgAGAAAGCGAAAAAAGGCGATGCAGGT‑AAAAAGGCGGCAAGCGTCGTCAGGTCGGTAAAAAGGTAAACTTTGAGCCAGACAGCGCCTTCCGCGGt                             <  1:773449/100‑1 (MQ=255)

                                                                    gcgcgAGAAAGCGAAAAAAGGCGATGCAGGT‑AAAAAGGCGGCAAGCGTCGTCAGGTCGTTAAAAAGGTAAACTTTGAGCCAGACAGCGCCTTCCGCGGTg                            <  2:1584876/100‑1 (MQ=255)

                                                                    gcgcgAGAAAGCGAAAAAAGGCGATGCAGGT‑AAAAAGGCGGCAAGCGTCGTCAGGTCGGTAAAAAGGTAAACTTTGAGCCAGACAGCGCCTTCCGCGGTg                            <  1:314810/100‑1 (MQ=255)

                                                                    gcgcgAGAAAGCGAAAAAAGGCGATGCAGGT‑AAAAAGGCGGCAAGCGTCGTCAGGTCGGTAAAAAGGTAAACTTTGAGCCAGACAGCGCCTTCCGCAGTg                            <  1:2190372/100‑1 (MQ=255)

                                                                      gcgAGAAAGCGAAAAAAGGCGATGCAGGT‑AAAAAGGCGGCAAGCGTCGTCAGGTCGGTAAAAAGGTAAACTTTGAGCCAGACAGCGCCTTCCGCGGTgag                          >  1:694876/1‑100 (MQ=255)

                                                                       cgAGAAAGCGAAAAAAGGCGATGCAGGT‑AAAAAGGCGGCAAGCGTCGTCAGGTCGGTAAAAAGGTAAACTTTGAGCCAGACAGCGCCTTCCGCGGTgaga                         >  1:914418/1‑100 (MQ=255)

                                                                        gagaAAGCGAAAAAAGGCGATGCAGGT‑AAAAAGGCGGCAAGCGTCGTCAGGTCGGTAAAAAGGTAAACTTTGAGCCAGACAGCGCCTTCCGCGGTGAGaa                        >  2:1650221/1‑100 (MQ=255)

                                                                         agaAAGCGAAAAAAGGCGATGCAGGTAAAAAAGGCGGCAAGCGTCGTCAGGTCGGTAAAAAGGTAAACTTTGAGCCAGACAGCGCCTTCCGCGGTGAGaa                        >  2:2794855/1‑100 (MQ=255)

                                                                           aaaGCGAAAAAAGGCGATGCAGGT‑AAAAAGGCGGCAAGCGTCGTCAGGTCGGTAAAAAGGTAAACTTTGAGCCAGACAGCGCCTTCCGCGGTGAGaaaaa                     <  1:1862503/100‑1 (MQ=255)

                                                                              gCGAAAAAAGGCGATGCAGGT‑AAAAAGGCGGCAAGCGTCGTCAGGTCGGTAAAAAGGTAAACTTTGAGCCAGACAGCGCCTTCCGCGGTGAGAAAAAAAc                  >  2:714795/1‑100 (MQ=255)

                                                                              gCGAAAAAAGGCGATGCAGGT‑AAAAAGGCGGCAAGCGTCGTCAGGTCGGTAAAAAGGTAAACTTTGAGCCAGACAGCGCCTTCCGCGGTGAGAAAAAAAc                  <  2:1879886/100‑1 (MQ=255)

                                                                              gCGAAAAAAGGCGATGCAGGT‑AAAAAGGCGGCAAGCGTCGTCAGGTCGGTAAAAAGGTAAACTTTGAGCCAGACAGCGCCTTCCGCGGTGAGAAAAAAAc                  >  1:1222530/1‑100 (MQ=255)

                                                                               cGAAAAAAGGCGATGCAGGT‑AAAAAGGCGGCAAGCGTCGTCAGGTCGGTAAAAAGGTAAACTTTGAGCCAGACAGCGCCTTCCGCGGTGAGAAAAAAACg                 >  2:89257/1‑100 (MQ=255)

                                                                               cGAAAAAAGGCGATGCAGGT‑AAAAAGGCGGCAAGCGTCGTCAGGTCGGTAAAAAGGTAAACTTTGAGCCAGACAGCGCCTTCCGCGGTGAGAAAAAAACg                 >  1:117851/1‑100 (MQ=255)

                                                                                gAAAAAAGGCGATGCAGGT‑AAAAAGGCGGCAAGCGTCGTCAGGTCGGTAAAAAGGTAAACTTTGAGCCAGACAGCGCCTTCCGCGGTGAGAAAAAAACGa                >  2:2799313/1‑100 (MQ=255)

                                                                                    aaaGGCGATGCAGGT‑AAAAAGGCGGCAAGCGTCGTCAGGTCGGTAAAAAGGTAAACTTTGAGCCAGACAGCGCCTTCCGCGGTGAGAAAAAAACGAAGcc            <  1:1614789/100‑1 (MQ=255)

                                                                                    aaaGGCGATGCAGGT‑AAAAAGGCGGCAAGCGTCGTCAGGTCGGTAAAAAGGTAAACTTTGAGCCAGACAGCGCCTTCCGCGGTGAGAAAAAAACGAAGcc            <  2:1663642/100‑1 (MQ=255)

                                                                                       ggCGATGCAGGT‑AAAAAGGCGGAAAGCGTCGTCAGGTCGGTACAAAGGTAACCTTTGAGCCAGACAGCGCCTTCCGCGGTGAGAAAAAAACGAAGCCGaa         <  1:853597/100‑1 (MQ=255)

                                                                                         cGATGCAGGT‑AAAAAGGCGGCAAGCGTCGTCAGGTCGGTAAAAAGGTAAACTTTGAGCCAGACAGCGCCTTCCGCGGTGAGAAAAAAACGAAGCCGAAAg       <  1:2541062/100‑1 (MQ=255)

                                                                                          gATGCAGGT‑AAAAAGGCGGCAAGCGTCGTCAGGTCGGTAAAAAGGTAAACTTTGAGCCAGACAGCGCCTTCCGCGGTGAGAAAAAAACGAAGCCGAAAgc      >  1:1725167/1‑100 (MQ=255)

                                                                                          gATGCAGGT‑AAAAAGGCGGCAAGCGTCGTCAGGTCGGTAAAAAGGTAAACTTTGAGCCAGACAGCGCCTTCCGCGGTGAGAAAAAAACGAAGCCGAAAgc      <  1:2867688/100‑1 (MQ=255)

                                                                                           aTGCAGGT‑AAAAAGGCGGCAAGCGTCGTCAGGTCGGTAAAAAGGTAAACTTTGAGCCAGACAGCGCCTTCCGCGGTGAGAAAAAAACGAAGCCGAAAgcg     >  1:1245026/1‑100 (MQ=255)

                                                                                             gCAGGT‑AAAAAGGCGGCAAGCGTCGTCAGGTCGGTAAAAAGGTAAACTTTGAGCCAGACAGCGCCTTCCGCGGTGAGAAAAAAACGAAGCCGAAAgcggc   <  2:1825512/100‑1 (MQ=255)

                                                                                              cAGGT‑AAAAAGGCGGCAAGCGTCGTCAGGTCGGTAAAAAGGTAAACTTTGAGCCAGACAGCGCCTTCCGCGGTGAGAAAAAAACGAAGCCGAAAgcggcg  >  1:1728408/1‑100 (MQ=255)

                                                                                              cAGGT‑AAAAAGGCGGCAAGCGTCGTCAGGTCGGTAAAAAGGTAAACTTTGAGCCAGACAGCGCCTTCCGCGGTGAGAAAAAAACGAAGCCGAAAgcggcg  >  1:1572038/1‑100 (MQ=255)

                                                                                              cAGGT‑AAAAAGGCGGCAAGCGTAGTCAGGTCGGTAAAAAGGTAAACTTTGAGCCAGACAGCGCCTTCCGCGGTGAGAAAAAAACGAAGCCGAAAgcggcg  <  2:2595277/100‑1 (MQ=255)

GACGAGCGCAAAATCGACTTTAGCCTGATCTCCAGCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGTAAAAAAGGCGGCAAGCGTCGTCAGGTCGGTAAAAAGGTAAACTTTGAGCCAGACAGCGCCTTCCGCGGTGAGAAAAAAACGAAGCCGAAAGCGGCG  >  NC_000913/4408799‑4408993

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A