Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A16 F258 I0 R1 432 103.0 5191697 92.7% 4812703 99.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,408,903 (A)6→5 100% coding (2250/2442 nt) rnr → exoribonuclease R, RNase R

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,408,8980A.84.7% 410.7 / 85.7 137coding (2245/2442 nt)rnrexoribonuclease R, RNase R
Reads supporting (aligned to +/- strand):  ref base A (10/11);  new base . (50/66);  total (60/77)
Fisher's exact test for biased strand distribution p-value = 8.12e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 145 total aligned reads displayed.

GAGCGCAAAATCGACTTTAGCCTGATCTCCAGCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGTAAAAAAGGCGGCAAGCGTCGTCAGGTCGGTAAAAAGGTAAACTTTGAGCCAGACAGCGCCTTCCGCGGTGAGAAAAAAACGAAGCCGAAAGCGGCGA  >  NC_000913/4408802‑4408994
                                                                                                |                                                                                                
gAGCGCAAAATCGACTTTAGCCTGATCTCCAGTGAACGCGCACCGCGCAACGTAGGTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGTaaaa                                                                                               <  2:966287/100‑1 (MQ=255)
   cgcAAAATCGACTTTAGCCTGATCTCCAGCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGTAAAAAAg                                                                                            >  1:31563/1‑100 (MQ=255)
    gcAAAATCGACTTTAGCCTGATCTCCAGCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGCAAGCGAAAAAAGGCGATGCAGGTaaaaaggc                                                                                           >  2:1607024/1‑97 (MQ=255)
    gcAAAATCGACTTTAGCCTGATCTCCAGCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGTaaaaaggc                                                                                           <  1:396819/100‑4 (MQ=255)
    gcAAAATCGACTTTAGCCTGATCTCCAGCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGTaaaaaggc                                                                                           <  1:1808683/100‑4 (MQ=255)
    gcAAAATCGACTTTAGCCTGATCTCCAGCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGTaaaaaggc                                                                                           >  2:1154673/1‑97 (MQ=255)
     cAAAATCGACTTTAGCCTGATCTCCAGCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGTAAAAAAggc                                                                                          >  2:177896/1‑100 (MQ=255)
      aaaaTCGACTTTAGCCTGATCTCCAGCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGT‑AAAAAggcgg                                                                                        <  1:2320944/100‑1 (MQ=255)
      aaaaTCGACTTTAGCCTGATCTCCAGCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGT‑AAAAAggcgg                                                                                        >  2:1413003/1‑100 (MQ=255)
      aaaaTCGACTTGAGCCTGACCTCCAGCGAACGCGGACCGCGCAACGTCGGTAAAACGGCGCTCAAGAAAGCGAAAAAAGGCGATGCAGGTaaaaaggcg                                                                                          <  2:1513651/99‑5 (MQ=255)
       aaaTCGACTTTAGCCTGATCTCCAGCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGTAAAAAAggcgg                                                                                        <  1:2058443/100‑1 (MQ=255)
       aaaTCGACTTTAGCCTGATCTCCAGCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGT‑AAAAAggcggc                                                                                       >  1:947275/1‑100 (MQ=255)
         aTCGACTTTAGCCTGATCTCCAGCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGT‑AAAAAGGCGGCaa                                                                                     <  2:578871/100‑1 (MQ=255)
          tCGACTTTAGCCTGATCTCCAGCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGT‑AAAAAGGCGGCAAg                                                                                    <  2:2206104/100‑1 (MQ=255)
           cGACTTTAGCCTGATCTCCAGCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGT‑AAAAAGGCGGCAAGc                                                                                   >  2:1043914/1‑100 (MQ=255)
            gACTTTAGCCTGATCTCCAGCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGT‑AAAAAGGCGGCAAGcg                                                                                  <  2:1245300/100‑1 (MQ=255)
            gACTTTAGCCTGATCTCCAGCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGT‑AAAAAGGCGGCAAGcg                                                                                  <  1:125291/100‑1 (MQ=255)
            gACTTTAGCCTGATCTCCAGCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGT‑AAAAAGGCGGCAAGcg                                                                                  >  2:233564/1‑100 (MQ=255)
              cTTTAGCCTGATCTCCAGCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGT‑AAAAAGGCGGCAAGcgtc                                                                                <  2:458568/100‑1 (MQ=255)
               tttAGCCTGATCTCCAGCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGTAAAAAAGGCGGCAAGcgtc                                                                                <  1:1322049/100‑1 (MQ=255)
                  aGCCTGATCTCCAGCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGTAAAAAAGGCGGCAAGCgtcgtc                                                                             <  2:2577978/100‑1 (MQ=255)
                   gCCTGATCTCCAGCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGT‑AAAAAGGCGGCAAGCGTCGTCAg                                                                           >  1:2177194/1‑100 (MQ=255)
                   gCCTGATCTCCAGCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGT‑AAAAAGGCGGCAAGCGTCGTCAg                                                                           >  1:433782/1‑100 (MQ=255)
                   gCCTGATCTCCAGAGAACGGGCAACTCTCAACGTGGGTACAACGGATAAAAAAAAAAAAAAAAAAGGCGATGCAGGT‑AAAAAGGCGGCAAGCGTCGTCa                                                                            <  1:1722650/99‑1 (MQ=255)
                    ccTGATCTCCAGCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGT‑AAAAAGGCGGCAAGCGTCGTCAgg                                                                          <  1:1644004/100‑1 (MQ=255)
                     cTGATCTCCAGCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGT‑AAAAAGGCGGCAAGCGTCGTCAGGt                                                                         >  1:734621/1‑100 (MQ=255)
                      tGATCTCCAGCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGAGGCAGGT‑AAAAAGGCGGCAAGCgtcgtc                                                                             >  1:2435276/1‑95 (MQ=255)
                       gATCTCCAGCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGTAAAAAAGGCGGCAAGCGTCGTCAGGTc                                                                        <  1:108079/100‑1 (MQ=255)
                       gATCTCCAGCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGT‑AAAAAGGCGGCAAGCGTCGTCAGGTCg                                                                       <  2:743321/100‑1 (MQ=255)
                        aTCTCCAGCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGT‑AAAAAGGCGGCAAGCGTCGTCAGGTCgg                                                                      <  2:1726903/100‑1 (MQ=255)
                        aTCTCCAGCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGT‑AAAAAGGCGGCAAGCGTCGTCAGGTCgg                                                                      >  2:1391714/1‑100 (MQ=255)
                        aTCTCCAGCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGT‑AAAAAGGCGGCAAGCGTCGTCAGGTCgg                                                                      <  1:1660479/100‑1 (MQ=255)
                        aTCTCCAGCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGT‑AAAAAGGCGGCAAGCGTCGTCAGGTCgg                                                                      <  2:271201/100‑1 (MQ=255)
                         tctcCAGCGAACGCGTACCGCGCAACGTCGGTAAAACGGAGCGCGAGAAAGCGAAAAAAGGCGATGCAGGTAAAAAAGGCGGCAAGCGTCGTCAGGTCgg                                                                      <  1:1740725/100‑1 (MQ=255)
                         tctcCAGCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGTAAAAAAGGCGGCAAGCGTCGTCAGGTCgg                                                                      <  1:582378/100‑1 (MQ=255)
                         tctcCAGCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGT‑AAAAAGGCGGCAAGCGTCGTCAGGTCGGt                                                                     <  2:899047/100‑1 (MQ=255)
                            ccAGCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGT‑AAAAAGGCGGCAAGCGTCGTCAGGTCGGTaaa                                                                  <  2:1629276/100‑1 (MQ=255)
                            ccAGCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGT‑AAAAAGGCGGCAAGCGTCGTCAGGTCGGTaaa                                                                  >  2:2485118/1‑100 (MQ=255)
                            ccAGCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGT‑AAAAAGGCGGCAAGCGTCGTCAGGTCGGTaaa                                                                  >  2:896806/1‑100 (MQ=255)
                            ccAGCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGT‑AAAAAGGCGGCAAGCGTCGTCAGGTCGGTaaa                                                                  >  2:1226920/1‑100 (MQ=255)
                             cAGCGAACGGGCACCGAGCAACGTCGGTAAAACGGCGAAAGAAAAAGAAAAAAAAGGCGATGCAGGT‑AAAAAGGCGGCAAGCGTCGTCAGGTCGGTaaaa                                                                 <  1:523823/100‑1 (MQ=255)
                             cAGCGAACGCGCACCGCCAAACGTCGGTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGT‑AAAAAGGCGGCAAGCGTCGTCAGGTc                                                                        <  1:596369/93‑1 (MQ=255)
                              aGCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGTAAAAAAGGCGGCAAGCGTCGTCAGGTCGGTaaaa                                                                 <  2:362633/100‑1 (MQ=255)
                              aGCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGT‑AAAAAGGCGGCAAGCGTCGTCAGGTCGGTaaaaa                                                                <  2:1188002/100‑1 (MQ=255)
                              aGCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGT‑AAAAAGGCGGCAAGCGTCGTCAGGTCGGTaaaaa                                                                <  1:49840/100‑1 (MQ=255)
                               gCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGT‑AAAAAGGCGGCAAGCGTCGTCAGGTCGGTAAAAAg                                                               <  1:229424/100‑1 (MQ=255)
                                cGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGT‑AAAAAGGCGGCAAGCGTCGTCAGGTCGGTAAAAAgg                                                              <  2:299842/100‑1 (MQ=255)
                                cGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGT‑AAAAAGGCGGCAAGCGTCGTCAGGTCGGTAAAAAgg                                                              >  2:8994/1‑100 (MQ=255)
                                 gAACGCGCACCGCGCAACGGCGGTAAACCGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGT‑AAAAAGGCGGCAAGCGTCGTCAGGTCGGTAAAAAGGt                                                             <  1:513803/100‑1 (MQ=255)
                                    cgcgCACCGCGCAACGTCGGTAAACCGGCGCGCGAGAAAGCGCAAACAGGCGATGCAGGT‑AAAAAGGCGGCAAGCGTCGTCAGGTCGGTAAAAAgg                                                              >  1:2545833/1‑96 (MQ=255)
                                    cgcgCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGTAAAAAAGGCGGCAAGCGTCGTCAGGTCGGTAAAAAGGTaa                                                           >  2:1196478/1‑100 (MQ=255)
                                    cgcgCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGT‑AAAAAGGCGGTAAGCGTCGTCAGGTCGGTAAAAAGGTaaa                                                          >  1:1559638/1‑100 (MQ=255)
                                    cgcgCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGT‑AAAAAGGCGGCAAGCGTCGTCAGGTCGGTAAAAAGGTaaa                                                          >  2:1137338/1‑100 (MQ=255)
                                     gcgcACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGT‑AAAAAGGCGGCAAGCGTCGTCAGGTCGGTAAAAAGGTAAAc                                                         <  2:1719583/100‑1 (MQ=255)
                                     gcgcACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGT‑AAAAAGGCGGCAAGCGTCGTCAGGTCGGTAAAAAGGTAAAc                                                         >  1:43773/1‑100 (MQ=255)
                                        cACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGT‑AAAAAGGCGGCAAGCGTCGTCAGGTCGGTAAAAAGGTAAACttt                                                      >  1:2525281/1‑100 (MQ=255)
                                            gcgcAACGTCGGTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGTAAAAAAGGAGGCAAGCGAAGTCAGGCCGGTAAAAAAGAAAACTTAGa                                                    >  1:2142432/1‑99 (MQ=255)
                                             cgcAACGTCGGTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGT‑AAAAAGGCGGCAAGCGTCGTCAGGTCGGTAAAAAGGTAAACTTTGAGcc                                                 <  1:896803/100‑1 (MQ=255)
                                              gcAACGTCGGTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGT‑AAAAAGGCGGCAAGCGTCGAAAGGTAAGTAAAAAGGTAAACTTTGAGcc                                                 <  1:1931386/99‑1 (MQ=255)
                                               cAACGTCGGTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGT‑AAAAAGGCGGCAAGCGTCGTCAGGTCGGTAAAAAGGTAAACTTTGAGCCAg                                               <  1:461751/100‑1 (MQ=255)
                                                aaCGTCGGTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGAAAAAAAGGCGGCAAGCGTCGTCAGGTCGGTAAAAAGGTAAACTTTGAGCCAg                                               <  1:1420193/100‑1 (MQ=255)
                                                 aCGTCGGTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGT‑AAAAAGGCGGCAAGCGTCGTCAGGTCGGTAAAAAGGTGAACTTTGAGCCAGAc                                             <  2:1217565/100‑1 (MQ=255)
                                                  cGTCGGTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGT‑AAAAAGGCGGCAAGCGTCGTCAGGTCGGTAAAAAGGTAAACTTTGAGCCAGACa                                            <  1:7661/100‑1 (MQ=255)
                                                   gTCGGTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGT‑AAAAAGGCGGCAAGCGTCGTCAGGTCGGTAAAAAGGTAAACTTTGAGCCAGACAg                                           <  1:146253/100‑1 (MQ=255)
                                                   gTCGGTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGT‑AAAAAGGCGGCAAGCGTCGTCAGGACGGTAAAAAGGTAAACTTTGAGCCAGACAg                                           >  1:1180924/1‑100 (MQ=255)
                                                    tCGGTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGT‑AAAAAGGCGGCAAGCGTCGTCAGGTCGGTAAAAAGGTAAACTTTGAGCCAGACAgc                                          <  2:1396176/100‑1 (MQ=255)
                                                      ggTAAAAGGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGT‑AAAAAGGCGGCAAGCGTCGTCAGGTCGGTAAAAAGGTAAACTTTGAGCCAGACAgcgc                                        <  1:768419/100‑1 (MQ=255)
                                                      ggTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGT‑AAAAAGGCGGCAAGCGTCGTCAGGTCGGTAAAAAGGTAAACTTTGAGCCAGACAgcgc                                        <  1:1866468/100‑1 (MQ=255)
                                                      ggTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGT‑AAAAAGGCGGCAAGCGTCGTCAGGTCGGTAAAAAGGTAAACTTTGAGCCAGACAgcgc                                        >  2:2315433/1‑100 (MQ=255)
                                                       gTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGT‑AAAAAGGCGGCAAGCGTCGTCAGGTCGGTAAAAAGGTAAACTTTGAGCCAGACAGCGcc                                       <  1:1228637/100‑1 (MQ=255)
                                                       gTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGT‑AAAAAGGCGGCAAGCGTCGTCAGGTCGGTAAAAAGGTAAACTTTGAGCCAGACAGCGcc                                       >  2:2242728/1‑100 (MQ=255)
                                                           aaCGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGT‑AAAAAGGCGGCAAGCGTCGTCAGGTCGGTAAAAAGGTAAACTTTGAGCCAGACAGCGCCTTcc                                   <  2:2345245/100‑1 (MQ=255)
                                                               gcgcgcGAGAAAGCGAAAAAAGGCGATGCAGGT‑AAAAGGGCGGCAAGCGTCGTCAGGTCGGTAAAAAGGAAAACTTTGAGCCAGACAGCGCCTACCGAgg                               >  1:459273/1‑100 (MQ=255)
                                                                cgcgcgAGAAAGCGAAAAAAGGCGATGCAGGT‑AAAAAGGCGGCAAGCGTCGTCAGGTCGGTAAAAAGGTAAACTTTGAGCCAGACAGCGCCTTCCGCGGt                              >  1:955045/1‑100 (MQ=255)
                                                                 gcgcgAGAAAGCGAAAAAAGGCGATGCAGGT‑AAAAAGGCGGCAAGCGTCGTCAGGTCGGTAAAAAGGTAAACTTTGAGCCAGACAGCGCCTTCCGCGGTg                             <  2:1264511/100‑1 (MQ=255)
                                                                 gcgcgAGAAAGCGAAAAAAGGCCATGCAGGT‑CAAAAGTCGGCAAGCGTCGTCAGGTCGGTAAAAAGGTAAACTTTGAGCCAGACAGCGCCTTCCGCGGTg                             <  1:1751130/100‑1 (MQ=255)
                                                                  cgcgAGAAAGCGAAAAAAGGCGATGCAGGT‑AAAAAGGCGGCAAGCGTCGTCAGGTCGGTAAAAAGGTAAACTTTGAGCCAGACAGCGCCCTCCGCGGTga                            >  1:36145/1‑100 (MQ=255)
                                                                    cgAGAAAGCGAAAAAAGGCGATGCAGGT‑AAAAAGGCGGCAAGCGTCGTCAGGTCGGTAAAAAGGTAAACTTTGAGCCAGACAGCGCCTTCCGCGGTgaga                          >  2:538263/1‑100 (MQ=255)
                                                                    cgAGAAAGCGAAAAAAGGCGATGCAGGT‑AAAAAGGCGGCAAGCGTCGTCAGGTCGGTAAAAAGGTAAACTTTGAGCCAGACAGCGCCTTCCGCGGTgaga                          >  1:2481489/1‑100 (MQ=255)
                                                                      agaAAGCGAAAAAAGGCGATGCAGGT‑AAAAAGGCGGCAAGCGTCGTCAGGTCGGTAAAAAGGTAAACTTTGAGCCAGACAGCGCCTTCCGCGGTGAGaaa                        <  1:1137334/100‑1 (MQ=255)
                                                                         aaGCGAAAAAAGGCGATGCAGGT‑AAAAAGGCGGCAAGCGTCGTCAGGTCGGTAAAAAGGTAAACTTTGAGCCAGACAGCGCCTTCCGCGGTGAGaaaaaa                     <  2:1288095/100‑1 (MQ=255)
                                                                           gCGAAAAAAGGCGATGCAGGT‑AAAAAGGCGGCAAGCGTCGTCAGGTCGGTAAAAAGGTAAACTTTGAGCCAGACAGCGCCTTCCGCGGTGAGAAAAAAAc                   <  2:1974008/100‑1 (MQ=255)
                                                                            cGAAAAAAGGCGATGCAGGTAAAAAAGGCGGCAAGCGTCGTCAGGTCGGTAAAAAGGTAAACTTTGAGCCAGACAGCGCCTTCCGCGGTGAGAAAAAAAc                   >  2:860976/1‑100 (MQ=255)
                                                                            cGAAAAAAGGCGATGCAGGT‑AAAAAGGCGGCAAGCGTCGTCAGGTCGGTAAAAAGGTAAACTTTGAGCCAGACAGCGCCTTCCGCGGTGAGAAAAAAACg                  >  1:193319/1‑100 (MQ=255)
                                                                              aaaaaaGGCGATGCAGGT‑AAAAAGGCGGCAAGCGTCGTCAGGTCGGTAAAAAGGTAAACTTTGAGCCAGACAGCGCCTTCCGCGGTGAGAAAAAAACGaa                >  1:1866707/1‑100 (MQ=255)
                                                                              aaaaaaGGCCATGCAGGT‑AAAAAGGCGGCCAGCGTCGTCAGGTCGGTAAAAAGGGAAACTTTGAGCCAGACAGCGCCTTCCGCGGTGAGaaaa                       <  1:1944731/93‑1 (MQ=255)
                                                                               aatgaGGCGATACAGGT‑AAAAAGGCGGCTATCGTCGTAATGGTGTTAAAAAAGTAAACTTTGCGCCAAACAGCCCAGTACGGAGTGAGAAAAAAACGAAg               <  1:387888/96‑1 (MQ=255)
                                                                                aaaaGGCGATGCAGGT‑AAAAAGGCGGCAAGCGTCGTCAGGTCGGTAAAAAGGTAAACTTTGAGCCAGACAGCGCCTTCCGCGGTGAGAAAAAAACGAAGc              >  1:2025931/1‑100 (MQ=255)
                                                                                aaaaGGCGATGCAGGT‑AAAAAGGCGGCAAGCGTCGTCAGGTCGGTAAAAAGGTAAACTTTGAGCCAGACAGCGCCTTCCGCGGTGAGAAAAAAACGAAGc              >  2:2052458/1‑100 (MQ=255)
                                                                                 aaaGGCGATGCAGGT‑AAAAAGGCGGCAAGCGTCGTCAGGTCGGTAAAAAGGTAAACTTTGAGCCAGACAGCGCCTTCCGCGGTGAGAAAAAAACGAAGcc             <  2:943742/100‑1 (MQ=255)
                                                                                   aGGCGATGCAGGT‑AAAAAGGCGGCAAGCGTCGTCAGGTCGGTAAAAAGGTAAACTTTGAGCCAGACAGCGCCTTCCGCGGTGAGAAAAAAACGAAGCCGa           <  1:1339698/100‑1 (MQ=255)
                                                                                   aGGCGATGCAGGT‑AAAAAGGCGGCAAGCGTCGTCAGGTCGGTAAAAAGGTAAACTTTGAGCCAGACAGCGCCTTCCGAGGTAAGAAAAAAACGAAGCCGa           <  1:1827106/100‑1 (MQ=255)
                                                                                      cGATGCAGGT‑AAAAAGGCGGCAAGCGTCGTCAGGTCGGTAAAAAGGTAAACTTTGAGCCAGACAGCGCCTTCCGCGGTGAGAAAAAAACGAAGCCGAAAg        <  2:782809/100‑1 (MQ=255)
                                                                                      cGATGCAGGT‑AAAAAGGCGGCAAGCGTCGTCAGGTCGGTAAAAAGGTAAACTTTGAGCCAGACAGCGCCTTCCGCGGTGAGAAAAAAACGAAGCCGAAAg        <  2:1368728/100‑1 (MQ=255)
                                                                                       gATGCAGGT‑AAAAAGGCGGCAAGCGTCGTCAGGTCGGTGAAAAGGTAAACTTTGAGCCTGACAGCGCCTTCCGCGGTGAGAAAAAAACGAAGCCGAAAgc       <  1:1412995/100‑1 (MQ=255)
                                                                                        aTGCAGGT‑AAAAAGGCGGCAAGCGTCGTCAGGTCGGTAAAAAGGTAAACTTTGAGCCAGACAGCGCCTTCCGCGGTGAGAAAAAAACGAAGCCGAAAgcg      >  2:2479215/1‑100 (MQ=255)
                                                                                          gCAGGT‑AAAAAGGCGGCAAGCGTCGTCAGGTCGGTAAAAAGGTAAACTTTGAGCCAGACAGCGCCTTCCGCGGTGAGAAAAAAACGAAGCCGAAAgcggn    <  1:2412563/100‑2 (MQ=255)
                                                                                          gCAGGT‑AAAAAGGCGGCAAGCGTCGTCAGGTCGGTAAAAAGGTAAACTTTGAGCCAGACAGCGCCTTCCGCGGTGAGAAAAAAACGAAGCCGAAAgcggc    >  1:720779/1‑100 (MQ=255)
                                                                                           cAGGT‑AAAAAGGCGGCAAGCGTCGTCAGGTCGGTAAAAAGGTAAACTTTGAGCCAGACAGCGCCTTCCGCGGTGAGAAAAAAACGAAGCCGAAAgcggcg   >  2:1930804/1‑100 (MQ=255)
                                                                                           cAGGT‑AAAAAGGCGGCAAGCGTCGTCAGGTCGGTAAAAAGGTAAACTTTGAGCCAGACAGCGCCTTCCGCGGTGAGAAAAAAACGAAGCCGAAAgcggcg   >  2:1041745/1‑100 (MQ=255)
                                                                                                |                                                                                                
GAGCGCAAAATCGACTTTAGCCTGATCTCCAGCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGTAAAAAAGGCGGCAAGCGTCGTCAGGTCGGTAAAAAGGTAAACTTTGAGCCAGACAGCGCCTTCCGCGGTGAGAAAAAAACGAAGCCGAAAGCGGCGA  >  NC_000913/4408802‑4408994

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A