Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A16 F258 I0 R1	432	103.0	5191697	92.7%	4812703	99.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,104,114	T→A	21.2%	I288F (ATC→TTC)	cpxA ←	sensory histidine kinase in two‑component regulatory system with CpxR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,104,114	0	T	A	21.2%	254.4 / 61.0	118	I288F (ATC→TTC)	cpxA	sensory histidine kinase in two‑component regulatory system with CpxR
Reads supporting (aligned to +/- strand): ref base T (48/45); new base A (15/10); total (63/55)
Fisher's exact test for biased strand distribution p-value = 5.04e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01

Only 100 of 118 total aligned reads displayed.

GTTATCCAGCACTTCACTCCACAACTGGTTGGCTTTGATGGTTTCGCTAACCAGCGCGTTTTTTTGCTGATTACGTGACATCACCAACAGATCGTTGATCATGCTGTCCAGACGTTGCGCTTCGGTTTCAATACGCTCCAGTTCCTTGCTTTCACCGCTACGACGGCGCAGTAACGCCGTACCCAGTTGCAGA  >  NC_000913/4104016‑4104208

gTTATCCAGCACTTCACTCCACAACTGGTTGGCTTTGATGGTTTCGCTAACCAGCGCGTTTTTTTGCTGATTACGTGACATCACCAACAGATCGTTGATc                                                                                               <  1:2586134/100‑1 (MQ=255)

gTTATCCAGCACTTCACTCCACAACTGGTTGGCTTTGATGGTTTCGCTAACCAGCGCGTTTTTTTGCTGATTACGTGACATCACCAACAGAACGTTGATc                                                                                               >  2:1563006/1‑100 (MQ=255)

 ttATCCAGCACTTCACTCCACAACTGGTTGGCTTTGATGGTTTCGCTAACCAGCGCGTTTTTTTGCTGATTACGTGATATCACCAACAGATCGTTGAACa                                                                                              <  1:1500834/100‑1 (MQ=255)

 ttATCCAGCACTTCACTCCACAACTGGTTGGCTTTGATGGTTTCGCTAACCAGCGCGTTTTTTTGCTGATTACGTGACATCACCAACAGAACGTTGATCa                                                                                              <  1:1306122/100‑1 (MQ=255)

  tATCCAGCACTTCACTCCACAACTGGTTGGCTTTGATGGTTTCGCTAACCAGCGCGTTTTTTTGCTGATTACGTGACATCACCAACAGATCGTTGAACAt                                                                                             >  2:800943/1‑100 (MQ=255)

    tCCAGCACTTCACTCCACAACTGGTTGGCTTTGATGGTTTCGCTAACCAGCGCGTTTTTTTGCTGATTACGTGACATCACCAACAGATCGTTGATCATGc                                                                                           <  1:1918929/100‑1 (MQ=255)

    tCCAGCACTTCACTCCACAACTGGTTGGCTTTGATGGTTTCGCTAACCAGCGCGTTTTTTTGCTGATTACGTGACATCACCAACAGAACGTTGATCATGc                                                                                           <  2:1247903/100‑1 (MQ=255)

     ccAGCACTTCACTCCACAACTGGTTGGCTTTGATGGTTTCGCTAACCAGCGCGTTTTTTTGCTGATTACGTGACATCACCAACAGATCGTTGATCATGCt                                                                                          >  2:800180/1‑100 (MQ=255)

     ccAGCACTTCACTCCACAACTGGTTGGCTTTGATGGTTTCGCTAACCAGCGCGTTTTTTTGCTGATTACGTGACATCACCAACAGATCGTTGATCATGCt                                                                                          <  2:1414392/100‑1 (MQ=255)

      cAGCACTTCACTCCACAACTGGTTGGCTTTGATGGTTTCGCTAACCAGCGCGTTTTTTTGCTGATTACGTGACATCACCAACAGATCGTTGATCATGCTg                                                                                         <  2:1427891/100‑1 (MQ=255)

          aCTTCACTCCACAACTGGTTGGCTTTGATGGTTTCGCTAACCAGCGCGTTTTTTTGCTGATTACGTGACATCACCAACAGATCGTTGATCATGCTGTCCa                                                                                     <  2:1835405/100‑1 (MQ=255)

           cTTCACTCCACAACTGGTTGGCTTTGATGGTTTCGCTAACCAGCGCGTTTTTTTGCTGATTACGTGACATCACCAACAGATCGTTGAACATGCTGTCCAg                                                                                    >  2:1220721/1‑100 (MQ=255)

             tCACTCCACAACTGGTTGGCTTTGATGGTTTCGCTAACCAGCGCGTTTTTTTGCTGATTACGTGACATCACCAACAGAACGTTGATCATGCTGTCCAGAc                                                                                  >  1:2562226/1‑100 (MQ=255)

               aCTCCACAACTGGTTGGCTTTGATGGTTTCGCTAACCAGCGCGTTTTTTTTTTTTTTTTTTTACATAACAAACAGATCGTTTATCATTCTGTACAGACg                                                                                 >  1:2026552/1‑99 (MQ=255)

                cTCCACAACTGGTTGGCTTTGATGGTTTCGCTAACCAGCGCGTTTTTTTGCTGATTACTTGACATCACCAACAGATCGTTGATCATGCTGTCCAGACGtt                                                                               >  1:1678640/1‑100 (MQ=255)

                cTCCACAACTGGTTGGCTTTGATGGTTTCGCTAACCAGCGCGTTTTTTTGCTGATTACGTGACATCACCAACAGAACGTTGATCATGCTGTCCAGACGtt                                                                               >  2:811807/1‑100 (MQ=255)

                  ccACAACTGGTTGGCTTTGATGGTTTCGCTAACCAGCGCGTTTTTTTGCTGATTACGTGACATCACCAACAGAACGTTGATCATGCTGTCCAGACGTTgc                                                                             <  1:902869/100‑1 (MQ=255)

                   cacaACTGGTTGGCTTTGATGGTTTCGCTAACCAGCGCGTTTTTTTGCTGATTACGTGACATCACCAACAGATCGTTGATCATGCTGTCCAGACGTTgcg                                                                            <  2:821883/100‑1 (MQ=255)

                    acaACTGGTTGGCTTTGATGGTTTCGCTAACCAGCGCGTTTTTTTGCTGATTACGTGACATCACCAACAGATCGTTGATCATGCTGTCCAGACGTTgcgc                                                                           >  2:194003/1‑100 (MQ=255)

                          ggTTGGCTTTGATGGTTTCGCTAACCAGCGCGTTTTTTTGCTGATTACGTGACATCACCAACAGATCGTTGATCATGCTGTCCAGACGTTGCGCTTCGGt                                                                     >  1:2014320/1‑100 (MQ=255)

                          ggTTGGCTTTGATGGTTTCGCTAACCAGCGCGTTTTTTTGCTGATTACGTGACATCACCAACAGAACGTTGATCATGCTGTCCAGACGTTGCGCTTCGGt                                                                     >  1:824810/1‑100 (MQ=255)

                             tGGCTTTGATGGTTTCGCTAACCAGCGCGTTTTTTTGCTGATTACGTGACATCACCAACAGATCGTTGAACATGCTGTCCAGACGTTGCGCTTCGGTTTc                                                                  <  1:1193951/100‑1 (MQ=255)

                              ggCTTTGATGGTTTCGCTAACCAGCGCGTTTTTTTGCTGATTACGTGACATCACCAACAGATCGTTGATCATGCTGTCCAGACGTTGCGCTTCGGTTTCa                                                                 <  2:849326/100‑1 (MQ=255)

                              ggCTTTGATGGTTTCGCTAACCAGCGCGTTTTTTTGCTGATTACGTGACATCACCAACAGATCGTTGATCATGCTGTCCAGACGTTGCGCTTCGGTTTCa                                                                 <  1:2342538/100‑1 (MQ=255)

                              ggCTTTGATGGTTTCGCTAACCAGCGCGTTTTTTTGCTGATTACGTGACATCACCAACAGATCGTTGATCATGCTGTCCAGACGTTGCGCTTCGGTTTCa                                                                 <  2:1570471/100‑1 (MQ=255)

                              ggCTTTGATGGTTTCGCTAACCAGCGCGTTTTTTTGCTGATTACGTGACATCACCAACAGATCGTTGATCATGCTGTCCAGACGTTGCGCTTCGGTTTCa                                                                 <  2:1218746/100‑1 (MQ=255)

                               gCTTTGATGGTTTCGCTAACCAGCGCGTTTTTTTGCTGATTACGTGACATCACCAACAGATCGTTGATCATGCTGTCCAGACGTTGCGCTTCGGTTTCaa                                                                <  1:33118/100‑1 (MQ=255)

                                cTTTGATGGTTTCGCTAACCAGCGCGTTTTTTTGCTGATTACGTGACATCACCAACAGATCGTTGAACATGCTGTCCAGACGTTGCGCTTCGGTTTCAAt                                                               <  1:1859989/100‑1 (MQ=255)

                                cTTTGATGGTTTCGCTAACCAGCGCGTTTTTTTGCTGATTACGTGACATCACCAACAGATCGTTGAACATGCTGTCCAGACGTTGCGCTTCGGTTTCAAt                                                               <  2:1900830/100‑1 (MQ=255)

                                   tGATGGTTTCGCTAACCAGCGCGTTTTTTTGCTGATTACGTGACATCACCAACAGATCGTTGATCATGCTGTCCAGACGTTGCGCTTCGGTTTCAATACg                                                            >  2:1688073/1‑100 (MQ=255)

                                   tGATGGTTTCGCTAACCAGCGCGTTTTTTTGCTGATTACGTGACATCACCAACAGATCGTTGATCATGCTGTCCAGACGTTGCGCTTCGGTTTCAATACg                                                            >  2:836587/1‑100 (MQ=255)

                                    gATGGTTTCGCTAACCAGCGCGTTTTTTTGCTGATTATGTGACATCACCAACAGATCGTTGATAATGCTGTTCAGACGTTGGGCTTCTGTTTCAATAc                                                             >  1:478837/1‑98 (MQ=255)

                                    gATGGTTTCGCTAACCAGCGCGTTTTTTTGCTGATTACGTGACATCACCAACAGAACGTTGATCATGCTGTCCAGACGTTGCGCTTCGGTTTCAATACGc                                                           <  2:581661/100‑1 (MQ=255)

                                     aTGGTTTCGCTAACCAGCGCGTTTTTTTGCTGATTACGTGACATCACCAACAGATCGTTGATCATGCTGTCCAGACGTTGCGCTTCGGTTTCAATACGCt                                                          <  1:1671628/100‑1 (MQ=255)

                                      tGGTTTCGCTAACCAGCGCGTTTTTTTGCTGATTACGTGACATCACCAACAGATCGTTGATCATGCTGTCCAGACGTTGCGCTTCGGTTTCAATACGCTc                                                         >  2:1805086/1‑100 (MQ=255)

                                       ggTTTCGCTAACCAGCGCGTTTTTTTGCTGATTACGTGACATCACCAACAGAACGTTGATCATGCTGTCCAGACGTTGCGCTTCGGTTTCAATACGCTcc                                                        >  1:1438412/1‑100 (MQ=255)

                                        gTTTCGCTAACCAGCGCGTTTTTTTGCTGATTACGTGACATCACCAACAGATCGTTGATCATGCTGTCCAGACGTTGCGCTTCGGTTTCAATACGCTCCa                                                       >  2:1791780/1‑100 (MQ=255)

                                         tttCGCTAACCAGCGCGTTTTTTTGCTGATTACGTGACATCACCAACAGATCGTTGAACATGCTGTCCAGACGTTGCGCTTCGGTTTCAATACGCTCCAg                                                      <  2:2305677/100‑1 (MQ=255)

                                          ttCGCTAACCAGCGCGTTTTTTTGCTGATTACGTGACATCACCAACAGATCGTTGATCATGCTGTCCAGACGTTGCGCTTCGGTTTCAATACGCTCCAGt                                                     <  2:2438058/100‑1 (MQ=255)

                                           tCGCTAACCAGCGCGTTTTTTTTTTTTTTTTTTTACATCACCAACAGATCGTTGAACATCCTGTCCAGACGTTGCGCTTCGGTTTCCATCCGc                                                           >  1:958577/1‑93 (MQ=255)

                                            cGCTAACCAGCGCGTTTTTTTGCTGATTACGTGACATCACCAACAGATCGTTGATCATGCTGTCCAGACGTTGCGCTTCGGTTTCAATACGCTCCAGTTc                                                   >  1:339560/1‑100 (MQ=255)

                                            cGCTAACCAGCGCGTTTTTTTGCTGATTACGTGACATCACCAACAGATCGTTGAACATGCTGTCCAGACGTTGCGCTTCGGTTTCAATACGCTCCAGTTc                                                   >  1:829546/1‑100 (MQ=255)

                                             gCTAACCAGCGCGTTTTTTTGCTGATTACGTGACATCACCAACAGATCGTTGATCATGCTGTCCAGACGTTGCGCTTCGGTTTCAATACGCTCCAGTTcc                                                  >  1:231737/1‑100 (MQ=255)

                                             gCTAACCAGCGCGTTTTTTTGCTGATTACGTGACATCACCAACAGATCGTTGATCATGCTGTCCAGACGTTGCGCTTCGGTTTCAATACGCTCCAGTTcc                                                  >  1:696239/1‑100 (MQ=255)

                                             gCTAACCAGCGCGTTTTTTTGCTGATTACGTGACATCACCAACAGATCGTTGAACATGCTGTCCAGACGTTGCGCTTCGGTTTCAATACGCTCCAGCTcc                                                  >  1:2488614/1‑100 (MQ=255)

                                             gCTAACCAGCGCGTTTTTTTGCTGATTACGTGACATCACCAACAGATCGTTGAACATGCTGTCCAGACGTTGCGCTTCGGTTTCAATACGATCCAGTTcc                                                  >  2:583029/1‑100 (MQ=255)

                                                  ccAGCGCGTTTTTTTGCTGATTACGTGACATCACCAACAGATCGTTGATCATGCTGTCCAGACGTTGCGCTTCGGTTTCAATACGCTCCAGTTCCTTGCt                                             >  2:1429611/1‑100 (MQ=255)

                                                   cAGCGCGTTTTTTTGCTGATTACGTGACATCACCAACAGATCGTTGATCATGCTGTCCAGACGTTGCGCTTCGGTTTCAATACGCTCCAGTTCCTTGCtt                                            <  1:781113/100‑1 (MQ=255)

                                                   cAGCGCGTTTTTTTGCTGATTACGTGACATCACCAACAGATCGTTGATCATGCTGTCCAGACGTTGCGCTTCGGTTTCAATACGCTCCAGTTCCTTGCtt                                            >  2:410889/1‑100 (MQ=255)

                                                   cAGCGCGTTTTTTTGCTGATTACGTGACATCACCAACAGATCGTTGATCATGCTGTCCAGACGTTGCGCTTCGGTTTCAATACGCTCCAGTTCCTTGCtn                                            <  2:2155555/100‑2 (MQ=255)

                                                     gcgcGTTTTTTTGCTGATTACGTGACATCACCAACAGATCGTTGATCATGCTGTCCAGACGTTGCGCTTCGGTTTCAATACGCTCCAGTTCCTTGCTTTc                                          <  2:2506836/100‑1 (MQ=255)

                                                     gcgcGTTTTTTTGCTGATTACGTGACATCACCAACAGATCGTTGATCATGCTGTCCAGACGTTGCGCTTCGGTTTCAATACGCTCCAGTTCCTTGCTTTc                                          >  2:2543998/1‑100 (MQ=255)

                                                      cgcgTTTTTTTGCTGATTACGTGACATCACCAACAGATCGTTGATCATGCTGTCCAGACGTTGCGCTTCGGTTTCAATACGCTCCAGTTCCTTGCTTTCa                                         >  1:384059/1‑100 (MQ=255)

                                                       gcgTTTTTTTGCTGATTACGTGACATCACCAACAGATCGTTGATCATGCTGTCCAGACGTTGCGCTTCGGTTTCAATACGCTCCAGTTCCTTGCTTTCAc                                        <  2:374749/100‑1 (MQ=255)

                                                        cgTTTTTTTGCTTATTACGTGACATCACCAACAGATCGTTGAACATGCTGTCCAGACGTTGCGCTTCGGTTTCAATACGCTCCAGTTCCTTGCTTTCAc                                        >  1:30975/1‑99 (MQ=255)

                                                        cgTTTTTTTGCTGATTACGTGACATCACCAACAGATCGTTGATCATGCTGTCCAGACGTTGCGCTTCGGTTTCAATACGCTCCAGTTCCTTGCTTTCAcc                                       >  1:753707/1‑100 (MQ=255)

                                                                tGCTGATTACGTGACATCACCAACAGATCGTTGAACATGCTGTCCAGACGTTGCGCTTCGGTTTCAATACGCTCCAGTTCCTTGCTTTCACCGCTacgac                               >  2:2319915/1‑100 (MQ=255)

                                                                 gCTGATTACGTGACATCACCAACAGATCGTTGATCATGCTGTCCAGACGTTGCGCTTCGGTTTCAATACGCTCCAGTTCCTTGCTTTCACCGCTacgac                               <  1:2009711/99‑1 (MQ=255)

                                                                  cTGATTACGTGACATCACCAACAGATCGTTGATCATGCTGCCCAGACGTTGCGCTTCGGTTTCAATACGCTCCAGTTCCTTGCTTTCACCGCTACGACgg                             <  1:2001324/100‑1 (MQ=255)

                                                                   tGATTACGTGACATCACCAACAGATCGTTGATCATGCTGTCCAGACGTTGCGCTTCGGTTTCAATACGCTCCAGTTCCTTGCTTTCACCGCTACGACGgc                            <  2:2341326/100‑1 (MQ=255)

                                                                   tGATTACGTGACATCACCAACAGATCGTTGATCATGCTGTCCAGACGTTGCGCTTCGGTTTCAATACGCTCCAGTTCCTTGCTTTCACCGCTACGACGgc                            >  2:2152557/1‑100 (MQ=255)

                                                                   tGATTACGTGACATCAACAACAGATCGGTGAACATGCTGTCGAGACTGTGCGCTTCGGTTTGAATACGCTCCACGTCCTTGCTTTCCCCGCTACGACgg                             >  1:1498004/1‑99 (MQ=255)

                                                                    gATTACGTGACATCACCAACAGATCGTTGATCATGCTGTCCAGACGTTGCGCTTCGGTTTCAATACGCTCCAGTTCCTTGCTTTCACCGCTACGACGgcg                           >  1:401245/1‑100 (MQ=255)

                                                                     aTTACGTGACATCACCAACAGATCGTTGATCATGCTGTCCAGACGTTGCGCTTCGGTTTCAATACGCTCCAGTTCCTTGCTTTCACCGCTACGACGgcgc                          <  2:1958289/100‑1 (MQ=255)

                                                                     aTTACGTGACATCACCAACAGATCGTTGATCATGCTGTCCAGACGTTGCGCTTCGGTTTCAATACGCTCCAGTTCCTTGCTTTCACCGCTACGACGgcgc                          <  1:34561/100‑1 (MQ=255)

                                                                      ttACGTGACATCACCAACAGATCGTTGATCATGCTGTCCAGACGTTGCGCTTCGGTTTCAATACGCTCCAGTTCCTTGCTTTCACCGCTACGACGGCGCa                         <  2:1338373/100‑1 (MQ=255)

                                                                      ttACGTGACATCACCAACAGATCGTTGAACATGCTGTCCAGACGTTGCGCTTCGGTTTCAATACGCTCCAGTTCCTTGCTTTCACCGCTACGACGGCGCa                         <  2:1552184/100‑1 (MQ=255)

                                                                        aCGTGACATCACCAACAGATCGTTGATCATGCTGTCCAGACGTTGCGCTTCGGTTTCAATACGCTCCAGTTCCTTGCTTTCACCGCTACGACGGCGCAGt                       <  2:770667/100‑1 (MQ=255)

                                                                        aCGTGACATCACCAACAGAACGTTGATCATGCTGTCCAGACGTTGCGCTTCGGTTTCAATACGCTCCAGTTCCTTGCTTTCACCGCTACGACGGCGCAGt                       >  1:716078/1‑100 (MQ=255)

                                                                          gTGACATCACCAACAGATCGTTGATCATGCTGTCCAGACGTTGCGCTTCGGTTTCAATACGCTGCAGTTCCTTGCTTTCACCGCTACGACGGCGCAGTaa                     >  2:1819702/1‑100 (MQ=255)

                                                                           tGACATCACCAACAGATCGTTGAACATGCTGTCCAGACGTTGCGCTTCGGTTTCAATACGCTCCAGTTCCTTGCTTTCACCGCTACGACGGCGCAGTAAc                    >  1:1830346/1‑100 (MQ=255)

                                                                            gACATCACCAACAGATCGTTGATCATGCTGTCCAGACGTTGCGCTTCGGTTTCAATACGCTCCAGTTCCTTGCTTTCACCGCTACGACGGCGCAGTAACg                   >  1:1426783/1‑100 (MQ=255)

                                                                            gACATCACCAACAGATCGTTGATCATGCTGTCCAGACGTTGCGCTTCGGTTTCAATACGCTCCAGTTCCTTGCTTTCACCGCTACGACGGCGCAGTAACg                   >  2:2595557/1‑100 (MQ=255)

                                                                            gACATCACCAACAGATCGTTGATCATGCTGTCCAGACGTTGCGCTTCGGTTTCAATACGCTCCAGTTCCTTGCTTTCACCGCTACGACGGCGCAGTAACg                   >  2:1207729/1‑100 (MQ=255)

                                                                            gACATCACCAACAGATCGTTGATCATGCTGTCCAGACGTTGCGCTTCGGTTTCAATACGCTCCAGTTCCTTGCTTTCACCGCTACGACGGCGCAGTAACg                   >  2:2595535/1‑100 (MQ=255)

                                                                            gACATCACCAACAGATCGTTGAACAGGCTGTCAATACGTTCCGCTTCGGTTTCAATACGCTCGAGTTCCGTGCTTTCACCGCTACGACGGCGCAGTaa                     <  1:368367/98‑1 (MQ=255)

                                                                             aCATCACCAACAGATCGTTGATCATGCTGTCCAGACGTTGCGCTTCGGTTTCAATACGCTCCAGTTCCTTGCTTTCACCGCTACGACGGCGCAGTAACGc                  <  1:1521902/100‑1 (MQ=255)

                                                                              cATCACCAACAGATCGTTGATCATGCTGTCCAGACGTTGCGCTTCGGTTTCAATACGCTCCAGTTCCTTGCTTTCACCGCTACGACGGCGCAGTAACGcc                 <  2:844625/100‑1 (MQ=255)

                                                                              cATCACCAACAGATCGTTGATCATGCTGTCCAGACGTTGCGCTTCGGTTTCAATACGCTCCAGTTCCTTGCTTTCACCGCTACGACGGCGCAGTAACGcc                 >  2:2446374/1‑100 (MQ=255)

                                                                              cATCACCAACAGATCGTTGAACATGCTGTCCAGACGTTGCGCTTCGGTTTCAATACGCTCCAGTTCCTTGCTTTCACCGCTACGACGGCGCAGTAACGcc                 >  1:278683/1‑100 (MQ=255)

                                                                              cATCACCAACAGAACGTTGATCATGCTGTCCAGCCGTTGCGCTTCGGTTTCAATACGCTCCAGTTCCTTGCTTTCACCGCTACGACGGCGCAGTAACg                   >  1:1098046/1‑98 (MQ=255)

                                                                               aTCACCAACAGATCGTTGATCATGCTGTCCAGACGTTGCGCTTCGGTTTCAATACGCTCCAGTTCCTTGCTTTCACCGCTACGACGGCGCAGTAACGCCg                <  2:1249925/100‑1 (MQ=255)

                                                                               aTCACCAACAGATCGTTGATCATGCTGTCCAGACGTTGCGCTTCGGTTTCAATACGCTCCAGTTCCTTGCTTTCACCGCTACGACGGCGCAGTAACGCCg                >  1:2067029/1‑100 (MQ=255)

                                                                                 cACCAACAGATCGTTGATCATGCTGTCCAGACGTTGCGCTTCGGTTTCAATACGCTCCAGTTCCTTGCTTTCACCGCTACGACGGCGCAGTAACGCCGTa              >  1:1827137/1‑100 (MQ=255)

                                                                                 cACCAACAGATCGTTGATCATGCTGTCCAGACGTTGCGCTTCGGTTTCAATACGCTCCAGTTCCTTGCTTTCACCGCTACGACGGCGCAGTAACGCCGTa              <  2:753710/100‑1 (MQ=255)

                                                                                  aCCAACAGATCGTTGATCATGCTGTCCAGACGTTGCGCTTCGGTTTCAATACGCTCCAGTTCCTTGCTTTCACCGCTACGACGGCGCAGTAACGCCGTAc             >  2:90696/1‑100 (MQ=255)

                                                                                  aCCAACAGATCGTTGAACATGCTGTCCAGACGTTGCGCTTCGGTTTCAATACGCTCCAGTTCCTTGCTTTCACCGCTACGACGGCGCAGTAACGCCGTAc             <  1:1220717/100‑1 (MQ=255)

                                                                                   ccAACCGAACGTTGATCATGCTGTCCAGACGTTGCGCTTCGGTTTCAATACGCTCCAGTTCCTTGCTTTCACCGCTACGACGGCGCAGTAACGCCGTAcc            >  1:2280682/1‑100 (MQ=255)

                                                                                   ccAACAGATCGTTGATCATGCTGTCCAGACGTTGCGCTTCGGTTTCAATACGCTCCAGTTCCTTGCTTTCACCGCTACGACGGCGCAGTAACGCCGTAcc            <  1:95152/100‑1 (MQ=255)

                                                                                   ccAACAGATCGTTGAACATGCTGTCCAGACGTTGCGCTTCGGTTTCAATACGCTCCAGTTCCTTGCTTTCACCGCTACGACGGCGCAGTAACGCCGTAcc            >  2:1724211/1‑100 (MQ=255)

                                                                                       cAGATCGTTGATCATGCTGTCCAGACGTTGCGCTTCGGTTTCAATACGCTCCAGTTCCTTGCTTTCACCGCTACGACGGCGCAGTAACGCCGTACCCAGt        <  2:921735/100‑1 (MQ=255)

                                                                                       cAGATCGTTGAACATGCTGTCCAGACGTTGCGCTTCGGTTTCAATACGCTCCAGTTCCTTGCTTTCACCGCTACGACGGCGCAGTAACGCCGTACCCAGt        >  1:2576817/1‑100 (MQ=255)

                                                                                        aGATCGTTGAACATGCTGTCCAGACGTTGCGCTTCGGTTTCAATACGCTCCAGTTCCTTGCTTTCACCACTACGACGGCGCAGTAACGCCGTACCCAGtt       >  2:1173489/1‑100 (MQ=255)

                                                                                         gATCGTTGATCATGCTGTCCAGACGTTGCGCTTCGGTTTCAATACGCTCCAGTTCCTTGCTTTCACCGCTACGACGGCGCAGTAACGCCGTACCCAGTTg      <  2:384061/100‑1 (MQ=255)

                                                                                          aacGTTGATCATGCTGTCCAGACGTTGCGCTTCGGTTTCAATACGCTCCAGTTCCTTGCTTTCACCGCTACGACGGCGCAGTAACGCCGTACCCAGTTGc     >  1:1294080/3‑100 (MQ=255)

                                                                                          aacGTTGATCATGCTGTCCAGACGTTGCGCTTCGGTCTCAATACGCTCCAGTTCCTTGCTTTCACCGCTACGACGGCGCAGTAACGCCGTACCCAGTTGc     <  1:992528/98‑1 (MQ=255)

                                                                                            cGTTGATCATGCTGTCCAGACGTTGCGCTTCGGTTTCAATACGCTCCAGTTCCTTGCTTTCACCGCTACGACGGCGCAGTAACGCCGTACCCAGTTGCAg   >  2:1645627/1‑100 (MQ=255)

                                                                                            cGTTGATCATGCTGTCCAGACGTTGCGCTTCGGTTTCAATACGCTCCAGTTCCTTGCTTTCACCGCTACGACGGCGCAGTAACGCCGTACCCAGTTGCAg   >  1:2074307/1‑100 (MQ=255)

                                                                                             gTTGATCATGCTGTCCAGACGTTGCGCTTCGGTTTCAATACGCTCCAGTTCCTTGCTTTCACCGCTACGACGGCGCAGTAACGCCGTACCCAGTTGCAGa  >  2:1011206/1‑100 (MQ=255)

                                                                                             gTTGAACATGCTGTCCAGACGTTGCGCTTCGGTTTCAATACGCTCCAGTTCCTTGCTTTCACCGCTACGACGGCGCAGTAACGCCGTACCCAGTTGCAGa  >  1:1262138/1‑100 (MQ=255)

GTTATCCAGCACTTCACTCCACAACTGGTTGGCTTTGATGGTTTCGCTAACCAGCGCGTTTTTTTGCTGATTACGTGACATCACCAACAGATCGTTGATCATGCTGTCCAGACGTTGCGCTTCGGTTTCAATACGCTCCAGTTCCTTGCTTTCACCGCTACGACGGCGCAGTAACGCCGTACCCAGTTGCAGA  >  NC_000913/4104016‑4104208

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A